9095946

(3-chloro-6-fluoro-1-benzothiophen-2-yl)-(4-methylpiperidin-1-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3057
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 24540043
SMILES CC1CCN(CC1)C(=O)C2=C(C3=C(S2)C=C(C=C3)F)Cl
Standardized SMILES CC1CCN(CC1)C(=O)c2sc3cc(F)ccc3c2Cl
Molecular weight 311.8021
ALogP 4.44
H-bond donor count 0
H-bond acceptor count 3
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.18
% growth inhibition (Hom. pool) 7.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24540043
Download HIP data (tab-delimited text)  (excel)
Gene:BOS1(YLR078C)|FD-Score:3.86|P-value:5.71E-5|Clearance:0.33||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:DIB1(YPR082C)|FD-Score:3.25|P-value:5.71E-4|Clearance:0.08||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:GAB1(YLR459W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.19||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:ORC6(YHR118C)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RAT1(YOR048C)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.17||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:SEC26(YDR238C)|FD-Score:4.67|P-value:1.53E-6|Clearance:0.43||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SMC1(YFL008W)|FD-Score:3.34|P-value:4.23E-4|Clearance:0.08||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TAF12(YDR145W)|FD-Score:4.93|P-value:4.18E-7|Clearance:0.43||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TFB4(YPR056W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.21||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:YJL195C(YJL195C_d)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:BOS1(YLR078C)|FD-Score:3.86|P-value:5.71E-5|Clearance:0.33||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:DIB1(YPR082C)|FD-Score:3.25|P-value:5.71E-4|Clearance:0.08||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:GAB1(YLR459W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.19||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:ORC6(YHR118C)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RAT1(YOR048C)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.17||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:SEC26(YDR238C)|FD-Score:4.67|P-value:1.53E-6|Clearance:0.43||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SMC1(YFL008W)|FD-Score:3.34|P-value:4.23E-4|Clearance:0.08||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TAF12(YDR145W)|FD-Score:4.93|P-value:4.18E-7|Clearance:0.43||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TFB4(YPR056W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.21||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:YJL195C(YJL195C_d)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24540043
Download HOP data (tab-delimited text)  (excel)
Gene:AAD4(YDL243C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:AQY2(YLL052C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ATG11(YPR049C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:CHS3(YBR023C)|FD-Score:-5.54|P-value:1.50E-8||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COG6(YNL041C)|FD-Score:5.98|P-value:1.14E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:9.02|P-value:9.68E-20||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EAF5(YEL018W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:FMO1(YHR176W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FRD1(YEL047C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GAD1(YMR250W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:HIS1(YER055C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HOP1(YIL072W)|FD-Score:-3.86|P-value:5.64E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HST2(YPL015C)|FD-Score:-4.54|P-value:2.77E-6||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HUA2(YOR284W)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IRC14(YOR135C_d)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:MKS1(YNL076W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:NRG1(YDR043C)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication Gene:OLA1(YBR025C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:OSW2(YLR054C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PCL8(YPL219W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PKH3(YDR466W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PST2(YDR032C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PYC1(YGL062W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RMD8(YFR048W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Cytosolic protein required for sporulation Gene:SNO2(YNL334C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:STP3(YLR375W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SUR4(YLR372W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TRM3(YDL112W)|FD-Score:4.3|P-value:8.40E-6||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:UBP12(YJL197W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VMA1(YDL185W)|FD-Score:3.75|P-value:8.83E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA7(YGR020C)|FD-Score:4.03|P-value:2.85E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YCL075W(YCL075W)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YER038W-A(YER038W-A_d)|FD-Score:6.18|P-value:3.15E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YGL101W(YGL101W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YHI9(YHR029C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YLL007C(YLL007C_p)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Putative protein of unknown function Gene:YOL014W(YOL014W_p)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Putative protein of unknown function Gene:YPR059C(YPR059C_d)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:AAD4(YDL243C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:AQY2(YLL052C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ATG11(YPR049C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:CHS3(YBR023C)|FD-Score:-5.54|P-value:1.50E-8||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COG6(YNL041C)|FD-Score:5.98|P-value:1.14E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:9.02|P-value:9.68E-20||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EAF5(YEL018W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:FMO1(YHR176W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FRD1(YEL047C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GAD1(YMR250W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:HIS1(YER055C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HOP1(YIL072W)|FD-Score:-3.86|P-value:5.64E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HST2(YPL015C)|FD-Score:-4.54|P-value:2.77E-6||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HUA2(YOR284W)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IRC14(YOR135C_d)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:MKS1(YNL076W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:NRG1(YDR043C)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication Gene:OLA1(YBR025C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:OSW2(YLR054C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PCL8(YPL219W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PKH3(YDR466W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PST2(YDR032C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PYC1(YGL062W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RMD8(YFR048W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Cytosolic protein required for sporulation Gene:SNO2(YNL334C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:STP3(YLR375W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SUR4(YLR372W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TRM3(YDL112W)|FD-Score:4.3|P-value:8.40E-6||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:UBP12(YJL197W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VMA1(YDL185W)|FD-Score:3.75|P-value:8.83E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA7(YGR020C)|FD-Score:4.03|P-value:2.85E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YCL075W(YCL075W)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YER038W-A(YER038W-A_d)|FD-Score:6.18|P-value:3.15E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YGL101W(YGL101W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YHI9(YHR029C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YLL007C(YLL007C_p)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Putative protein of unknown function Gene:YOL014W(YOL014W_p)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Putative protein of unknown function Gene:YPR059C(YPR059C_d)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR145W4.934.18E-70.43TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YDR238C4.671.53E-60.43SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YOR048C4.241.12E-50.17RAT1Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
YPR056W4.072.34E-50.21TFB4Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
YLR078C3.865.71E-50.33BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
YLR459W3.532.07E-40.19GAB1GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
YFL008W3.344.23E-40.08SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YPR082C3.255.71E-40.08DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YJL195C_d3.187.46E-40.10YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YBL041W3.070.001060.05PRE7Beta 6 subunit of the 20S proteasome
YDL166C3.030.001240.01FAP7Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D
YGR145W3.020.001270.04ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YBR152W2.970.001470.03SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YOR232W2.940.001650.01MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YMR239C2.930.001680.00RNT1Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML071C9.029.68E-20COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YER038W-A_d6.183.15E-10YER038W-A_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria
YNL041C5.981.14E-9COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YPR059C_d5.561.33E-8YPR059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
YDL006W5.161.23E-7PTC1Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation
YEL018W4.671.50E-6EAF5Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex
YOR284W4.612.01E-6HUA2Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
YDL112W4.308.40E-6TRM32'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
YOR135C_d4.191.41E-5IRC14_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
YDR466W4.112.00E-5PKH3Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
YNL334C4.042.64E-5SNO2Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
YGR020C4.032.85E-5VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YDR032C3.993.33E-5PST2Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
YMR250W3.953.85E-5GAD1Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
YPR049C3.924.48E-5ATG11Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS)

GO enrichment analysis for SGTC_3057
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1701.72E-39SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.111111Golgi
0.1673.36E-38SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.0909091Golgi
0.1645.73E-37SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0895522Golgi
0.1578.17E-34SGTC_3660181-0206 4.3 μMChemDiv (Drug-like library)2748740.0909091Golgi
0.1526.64E-32SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0447761heme requiring
0.1504.13E-31SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.107692Golgi
0.1478.13E-30SGTC_1078idebenone 36.1 μMMiscellaneous36860.0441176Golgi
0.1461.66E-29SGTC_2740felodipine 17.1 μMMiscellaneous33330.148649
0.1391.22E-26SGTC_40960-0234 25.7 μMChemDiv (Drug-like library)68211120.0823529Golgi
0.1395.98E-27SGTC_1669st015249 58.4 μMTimTec (Natural product derivative library)7227930.15493Golgi
0.1391.11E-26SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.1Golgi
0.1374.82E-26SGTC_33259138760 55.2 μMChembridge (Drug-like library)438449590.121951Golgi
0.1369.12E-26SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.0555556Golgi
0.1369.03E-26SGTC_30539095903 49.5 μMChembridge (Drug-like library)171740180.135135Golgi
0.1339.29E-25SGTC_980074-0023 107.7 μMChemDiv (Drug-like library)35638210.104478Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3024909063149.47 μM0.61538525236383Chembridge (Drug-like library)311.8021034.43603
SGTC_297190921869.93 μM0.44067825236400Chembridge (Drug-like library)343.271343.6313ergosterol biosynthesis
SGTC_3152909925349.47 μM0.43333325236131Chembridge (Drug-like library)322.852863.45213NEO1-PIK1
SGTC_297490944409.66 μM0.4262325236134Chembridge (Drug-like library)387.722343.71413ergosterol biosynthesis
SGTC_2973909466517.96 μM0.41269825237061Chembridge (Drug-like library)336.879443.90813NEO1
SGTC_3147909702749.47 μM0.39655225236841Chembridge (Drug-like library)299.430444.66202
SGTC_3154910011349.47 μM0.37525237525Chembridge (Drug-like library)336.879444.39713
SGTC_3021908765849.47 μM0.33898325237042Chembridge (Drug-like library)275.38593.58802
SGTC_2910794840525.97 μM0.3333332971328Chembridge (Drug-like library)417.748323.49214plasma membrane duress
SGTC_3155909926549.47 μM0.32307725236499Chembridge (Drug-like library)330.83184.15213