1327-0027

N-benzyl-4-[bromo(dichloro)methyl]sulfonyl-2-nitroaniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3060
Screen concentration 12.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 16000455
SMILES C1=CC=C(C=C1)CNC2=C(C=C(C=C2)S(=O)(=O)C(Cl)(Cl)Br)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1cc(ccc1NCc2ccccc2)S(=O)(=O)C(Cl)(Cl)Br
Molecular weight 454.1231
ALogP 5.75
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 24.83
% growth inhibition (Hom. pool) 19.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16000455
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:6.47|P-value:4.86E-11|Clearance:0.15||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP9(YMR033W)|FD-Score:4.09|P-value:2.12E-5|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.17||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCT7(YJL111W)|FD-Score:-3.3|P-value:4.87E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:GPI14(YJR013W)|FD-Score:6.32|P-value:1.29E-10|Clearance:2.23||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:NMD3(YHR170W)|FD-Score:-4.5|P-value:3.47E-6|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRP2(YNR011C)|FD-Score:3.26|P-value:5.48E-4|Clearance:0.02||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RIM2(YBR192W)|FD-Score:3.9|P-value:4.76E-5|Clearance:0.64||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB7(YDR404C)|FD-Score:3.24|P-value:5.89E-4|Clearance:0.07||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL10(YLR075W)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPN7(YPR108W)|FD-Score:-3.19|P-value:7.06E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:SPC105(YGL093W)|FD-Score:19.8|P-value:9.37E-88|Clearance:13.35||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SUP35(YDR172W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.13||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YTH1(YPR107C)|FD-Score:34|P-value:3.22E-253|Clearance:14.15||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:6.47|P-value:4.86E-11|Clearance:0.15||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP9(YMR033W)|FD-Score:4.09|P-value:2.12E-5|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.17||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCT7(YJL111W)|FD-Score:-3.3|P-value:4.87E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:GPI14(YJR013W)|FD-Score:6.32|P-value:1.29E-10|Clearance:2.23||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:NMD3(YHR170W)|FD-Score:-4.5|P-value:3.47E-6|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRP2(YNR011C)|FD-Score:3.26|P-value:5.48E-4|Clearance:0.02||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RIM2(YBR192W)|FD-Score:3.9|P-value:4.76E-5|Clearance:0.64||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB7(YDR404C)|FD-Score:3.24|P-value:5.89E-4|Clearance:0.07||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL10(YLR075W)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPN7(YPR108W)|FD-Score:-3.19|P-value:7.06E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:SPC105(YGL093W)|FD-Score:19.8|P-value:9.37E-88|Clearance:13.35||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SUP35(YDR172W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.13||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YTH1(YPR107C)|FD-Score:34|P-value:3.22E-253|Clearance:14.15||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16000455
Download HOP data (tab-delimited text)  (excel)
Gene:ARO1(YDR127W)|FD-Score:7.91|P-value:1.30E-15||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARR2(YPR200C)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:BAP2(YBR068C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:16.9|P-value:1.21E-64||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BDF2(YDL070W)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BUD3(YCL014W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CAN1(YEL063C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:4.45|P-value:4.23E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DBP7(YKR024C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DUG2(YBR281C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF7(YNL136W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EFM1(YHL039W)|FD-Score:-3.83|P-value:6.35E-5||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERD1(YDR414C)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:EST3(YIL009C-A)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FBP26(YJL155C)|FD-Score:4.38|P-value:5.89E-6||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FSH3(YOR280C)|FD-Score:4.91|P-value:4.51E-7||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FYV10(YIL097W)|FD-Score:5.22|P-value:8.74E-8||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GYP1(YOR070C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAC1(YFL031W)|FD-Score:9.03|P-value:8.49E-20||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HSM3(YBR272C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:ICL2(YPR006C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:ILV1(YER086W)|FD-Score:6.27|P-value:1.83E-10||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IMP2'(YIL154C)|FD-Score:8.23|P-value:9.61E-17||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC4(YDR540C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRE1(YHR079C)|FD-Score:10.1|P-value:3.25E-24||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LSM12(YHR121W)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MPC2(YHR162W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRPL22(YNL177C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSE1(YOL033W)|FD-Score:-4.58|P-value:2.35E-6||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:NHX1(YDR456W)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:OXA1(YER154W)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:OYE2(YHR179W)|FD-Score:4.28|P-value:9.19E-6||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PCL6(YER059W)|FD-Score:4.31|P-value:8.23E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PDR1(YGL013C)|FD-Score:12.8|P-value:5.70E-38||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PET20(YPL159C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PPT2(YPL148C)|FD-Score:-3.75|P-value:8.73E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRS3(YHL011C)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RIM1(YCR028C-A)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPA12(YJR063W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPS7A(YOR096W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM22(YKL155C)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTG2(YGL252C)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SEC66(YBR171W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLM5(YCR024C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLT2(YHR030C)|FD-Score:14.3|P-value:7.06E-47||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOD2(YHR008C)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPP1(YPL138C)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:SPT23(YKL020C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SPT3(YDR392W)|FD-Score:8.25|P-value:8.02E-17||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SSN3(YPL042C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STO1(YMR125W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:THR1(YHR025W)|FD-Score:11.9|P-value:5.07E-33||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:8.44|P-value:1.57E-17||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRP3(YKL211C)|FD-Score:8.4|P-value:2.23E-17||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.37|P-value:8.85E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:17.1|P-value:3.90E-66||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX4(YMR067C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:URE2(YNL229C)|FD-Score:11|P-value:1.87E-28||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YAP1(YML007W)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL183C(YDL183C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR417C(YDR417C_d)|FD-Score:-4.54|P-value:2.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YDR537C(YDR537C_d)|FD-Score:7.19|P-value:3.33E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEN1(YER041W)|FD-Score:5.89|P-value:1.96E-9||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFH7(YFR007W)|FD-Score:7.59|P-value:1.65E-14||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YFL032W(YFL032W_d)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFR016C(YFR016C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene Gene:YHL045W(YHL045W_d)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YJR129C(YJR129C_p)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YMR315W(YMR315W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNL235C(YNL235C_d)|FD-Score:5.46|P-value:2.36E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL050C(YOL050C_d)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPR146C(YPR146C_d)|FD-Score:9.01|P-value:1.07E-19||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR197C(YPR197C_d)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ARO1(YDR127W)|FD-Score:7.91|P-value:1.30E-15||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARR2(YPR200C)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:BAP2(YBR068C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:16.9|P-value:1.21E-64||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BDF2(YDL070W)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BUD3(YCL014W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CAN1(YEL063C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:4.45|P-value:4.23E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DBP7(YKR024C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DUG2(YBR281C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF7(YNL136W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EFM1(YHL039W)|FD-Score:-3.83|P-value:6.35E-5||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERD1(YDR414C)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:EST3(YIL009C-A)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FBP26(YJL155C)|FD-Score:4.38|P-value:5.89E-6||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FSH3(YOR280C)|FD-Score:4.91|P-value:4.51E-7||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FYV10(YIL097W)|FD-Score:5.22|P-value:8.74E-8||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GYP1(YOR070C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAC1(YFL031W)|FD-Score:9.03|P-value:8.49E-20||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HSM3(YBR272C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:ICL2(YPR006C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:ILV1(YER086W)|FD-Score:6.27|P-value:1.83E-10||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IMP2'(YIL154C)|FD-Score:8.23|P-value:9.61E-17||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC4(YDR540C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRE1(YHR079C)|FD-Score:10.1|P-value:3.25E-24||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LSM12(YHR121W)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MPC2(YHR162W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRPL22(YNL177C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSE1(YOL033W)|FD-Score:-4.58|P-value:2.35E-6||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:NHX1(YDR456W)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:OXA1(YER154W)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:OYE2(YHR179W)|FD-Score:4.28|P-value:9.19E-6||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PCL6(YER059W)|FD-Score:4.31|P-value:8.23E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PDR1(YGL013C)|FD-Score:12.8|P-value:5.70E-38||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PET20(YPL159C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PPT2(YPL148C)|FD-Score:-3.75|P-value:8.73E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRS3(YHL011C)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RIM1(YCR028C-A)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPA12(YJR063W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPS7A(YOR096W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM22(YKL155C)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTG2(YGL252C)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SEC66(YBR171W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLM5(YCR024C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLT2(YHR030C)|FD-Score:14.3|P-value:7.06E-47||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOD2(YHR008C)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPP1(YPL138C)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:SPT23(YKL020C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SPT3(YDR392W)|FD-Score:8.25|P-value:8.02E-17||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SSN3(YPL042C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STO1(YMR125W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:THR1(YHR025W)|FD-Score:11.9|P-value:5.07E-33||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:8.44|P-value:1.57E-17||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRP3(YKL211C)|FD-Score:8.4|P-value:2.23E-17||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.37|P-value:8.85E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:17.1|P-value:3.90E-66||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX4(YMR067C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:URE2(YNL229C)|FD-Score:11|P-value:1.87E-28||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YAP1(YML007W)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL183C(YDL183C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR417C(YDR417C_d)|FD-Score:-4.54|P-value:2.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YDR537C(YDR537C_d)|FD-Score:7.19|P-value:3.33E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEN1(YER041W)|FD-Score:5.89|P-value:1.96E-9||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFH7(YFR007W)|FD-Score:7.59|P-value:1.65E-14||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YFL032W(YFL032W_d)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFR016C(YFR016C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene Gene:YHL045W(YHL045W_d)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YJR129C(YJR129C_p)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YMR315W(YMR315W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNL235C(YNL235C_d)|FD-Score:5.46|P-value:2.36E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL050C(YOL050C_d)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPR146C(YPR146C_d)|FD-Score:9.01|P-value:1.07E-19||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR197C(YPR197C_d)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C34.003.22E-25314.10YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YGL093W19.809.37E-8813.40SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YNR016C6.474.86E-110.15ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YJR013W6.321.29E-102.23GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YMR033W4.092.12E-50.02ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YHR036W4.072.33E-50.17BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YBR192W3.904.76E-50.64RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YNR011C3.265.48E-40.02PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YDR404C3.245.89E-40.07RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YDR172W3.187.46E-40.13SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YFL005W3.040.001170.12SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YPL124W2.920.001770.07SPC29Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
YOR077W2.850.002180.06RTS2Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
YGL116W2.800.002590.01CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YDL092W2.790.002660.12SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A17.103.90E-66TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YJL095W16.901.21E-64BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YHR030C14.307.06E-47SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YGL013C12.805.70E-38PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YHR025W11.905.07E-33THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YNL229C11.001.87E-28URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YHR079C10.103.25E-24IRE1Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
YFL031W9.038.49E-20HAC1Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
YPR146C_d9.011.07E-19YPR146C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR053W8.441.57E-17THR4Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
YKL211C8.402.23E-17TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR392W8.258.02E-17SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YIL154C8.239.61E-17IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YDR127W7.911.30E-15ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YFR007W7.591.65E-14YFH7Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases

GO enrichment analysis for SGTC_3060
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.7700SGTC_30641327-0027 10.0 μMChemDiv (Drug-like library)160004551
0.7670SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.563636
0.7420SGTC_30591327-0034 3.0 μMChemDiv (Drug-like library)159985800.563636
0.4552.26E-297SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.0833333SWF1 & branched chain AA biosynthesis
0.4002.26E-224SGTC_24295902844 64.4 μMMiscellaneous19277900.0694444
0.3646.53E-183SGTC_1420335-0849 62.7 μMChemDiv (Drug-like library)38890430.146667ubiquinone biosynthesis & proteasome
0.3621.59E-181SGTC_1370438-0332 85.4 μMChemDiv (Drug-like library)20633930.047619ubiquinone biosynthesis & proteasome
0.3623.78E-181SGTC_8520418-0143 439.0 μMChemDiv (Drug-like library)53692290.0142857calcium & mitochondrial duress
0.3407.97E-159SGTC_216k038-0035 28.4 μMChemDiv (Drug-like library)44442310.0405405ubiquinone biosynthesis & proteasome
0.3297.65E-148SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0724638
0.3268.69E-145SGTC_30229087993 49.5 μMChembridge (Drug-like library)171736440.0722892
0.3238.63E-143SGTC_12941000-1494 117.0 μMChemDiv (Drug-like library)7127970.191176
0.3212.13E-140SGTC_2651TUB2.036 5.0 μMChemDiv (Drug-like library)159922070.574074
0.3076.25E-128SGTC_294k038-0071 157.0 μMChemDiv (Drug-like library)241528260.0384615ubiquinone biosynthesis & proteasome
0.3002.95E-122SGTC_12390337-0584 35.4 μMChemDiv (Drug-like library)9143010.196721unfolded protein response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_30641327-002710 μM116000455ChemDiv (Drug-like library)454.123145.74815
SGTC_1631327-00367.55 μM0.57407415992207ChemDiv (Drug-like library)432.117625.56615
SGTC_26511327-00365 μM0.57407415992207ChemDiv (Drug-like library)432.117625.56615
SGTC_30591327-00343 μM0.56363615998580ChemDiv (Drug-like library)446.14426.02315
SGTC_30631327-00344 μM0.56363615998580ChemDiv (Drug-like library)446.14426.02315
SGTC_797k781-0471270 μM0.43103423738129ChemDiv (Drug-like library)306.701143.4125RPP1 & pyrimidine depletion
SGTC_225nsc-1226571.02 μM0.350877275685Miscellaneous322.29332.72806
SGTC_6520437-0092114 μM0.3289473091274ChemDiv (Drug-like library)507.775385.60636
SGTC_2816798270658.44 μM0.3235292981853Chembridge (Drug-like library)326.392883.50115azole & statin
SGTC_260302-0163425.16 μM0.3103453100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_458nppb133 μM0.3076924549ICCB bioactive library300.309243.52325
SGTC_1764st04450293.5 μM0.3076928407TimTec (Natural product derivative library)214.223.27413