glucosamine

(2R,3R,4S,5R)-2-amino-3,4,5,6-tetrahydroxyhexanal hydrochloride

and experimental redox chemotherapeutic

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3061
Screen concentration 200.0 μM
Source Miscellaneous
PubChem CID 91431
SMILES C(C(C(C(C(C=O)N)O)O)O)O.Cl
Standardized SMILES NC(C=O)C(O)C(O)C(O)CO
Molecular weight 215.6321
ALogP -2.7
H-bond donor count 6
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) -0.2
% growth inhibition (Hom. pool) -4.94


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 91431
Download HIP data (tab-delimited text)  (excel)
Gene:ALG13(YGL047W)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.01||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ILV5(YLR355C)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRA1(YBR140C)|FD-Score:3.82|P-value:6.57E-5|Clearance:0.38||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MGE1(YOR232W)|FD-Score:-4.15|P-value:1.69E-5|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NUG1(YER006W)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.38||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUS1(YDL193W)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.05||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRP28(YDR243C)|FD-Score:3.1|P-value:9.56E-4|Clearance:0.11||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:RIO2(YNL207W)|FD-Score:-3.19|P-value:7.07E-4|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPS15(YOL040C)|FD-Score:3.72|P-value:9.98E-5|Clearance:0.38||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SEC26(YDR238C)|FD-Score:-3.36|P-value:3.85E-4|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TAD3(YLR316C)|FD-Score:-3.3|P-value:4.79E-4|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:YLR339C(YLR339C_d)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.38||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:ALG13(YGL047W)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.01||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ILV5(YLR355C)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRA1(YBR140C)|FD-Score:3.82|P-value:6.57E-5|Clearance:0.38||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MGE1(YOR232W)|FD-Score:-4.15|P-value:1.69E-5|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NUG1(YER006W)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.38||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUS1(YDL193W)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.05||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRP28(YDR243C)|FD-Score:3.1|P-value:9.56E-4|Clearance:0.11||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:RIO2(YNL207W)|FD-Score:-3.19|P-value:7.07E-4|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPS15(YOL040C)|FD-Score:3.72|P-value:9.98E-5|Clearance:0.38||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SEC26(YDR238C)|FD-Score:-3.36|P-value:3.85E-4|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TAD3(YLR316C)|FD-Score:-3.3|P-value:4.79E-4|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:YLR339C(YLR339C_d)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.38||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 91431
Download HOP data (tab-delimited text)  (excel)
Gene:AIM17(YHL021C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ASN2(YGR124W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG11(YPR049C)|FD-Score:-4.85|P-value:6.29E-7||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BRE1(YDL074C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CCZ1(YBR131W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CPR1(YDR155C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress Gene:CTP1(YBR291C)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:ECM23(YPL021W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EMP65(YER140W_p)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE8(YLR047C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:FYV1(YDR024W_d)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GAS1(YMR307W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN2(YDR283C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN4(YEL009C)|FD-Score:4.51|P-value:3.30E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HAP5(YOR358W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:IMP2'(YIL154C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IST2(YBR086C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:MAD2(YJL030W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MET17(YLR303W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MPD1(YOR288C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:NOT3(YIL038C)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PUB1(YNL016W)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RAD33(YML011C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RPL43B(YJR094W-A)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS4B(YHR203C)|FD-Score:-4.28|P-value:9.45E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:SAC6(YDR129C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEA4(YBL104C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SSM4(YIL030C)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SUL2(YLR092W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWF1(YDR126W)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TDA5(YLR426W_p)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:UBP12(YJL197W)|FD-Score:6.11|P-value:4.89E-10||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VHS1(YDR247W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:YAL037W(YAL037W_p)|FD-Score:5.23|P-value:8.33E-8||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YAP5(YIR018W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBR012C(YBR012C_d)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YDR379C-A(YDR379C-A)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YFR020W(YFR020W_p)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL177W(YGL177W_d)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR115W(YJR115W_p)|FD-Score:-3.13|P-value:8.76E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YLR224W(YLR224W_p)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YLR312C(YLR312C_p)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Putative protein of unknown function Gene:YMR130W(YMR130W_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YMR317W(YMR317W_p)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YOR139C(YOR139C_d)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL025C(YPL025C_d)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL080C(YPL080C_d)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL113C(YPL113C_p)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:ZRG8(YER033C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ZWF1(YNL241C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AIM17(YHL021C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ASN2(YGR124W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG11(YPR049C)|FD-Score:-4.85|P-value:6.29E-7||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BRE1(YDL074C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CCZ1(YBR131W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CPR1(YDR155C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress Gene:CTP1(YBR291C)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:ECM23(YPL021W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EMP65(YER140W_p)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE8(YLR047C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:FYV1(YDR024W_d)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GAS1(YMR307W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN2(YDR283C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN4(YEL009C)|FD-Score:4.51|P-value:3.30E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HAP5(YOR358W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:IMP2'(YIL154C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IST2(YBR086C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:MAD2(YJL030W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MET17(YLR303W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MPD1(YOR288C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:NOT3(YIL038C)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PUB1(YNL016W)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RAD33(YML011C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RPL43B(YJR094W-A)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS4B(YHR203C)|FD-Score:-4.28|P-value:9.45E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:SAC6(YDR129C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEA4(YBL104C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SSM4(YIL030C)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SUL2(YLR092W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SWF1(YDR126W)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TDA5(YLR426W_p)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:UBP12(YJL197W)|FD-Score:6.11|P-value:4.89E-10||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VHS1(YDR247W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:YAL037W(YAL037W_p)|FD-Score:5.23|P-value:8.33E-8||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YAP5(YIR018W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBR012C(YBR012C_d)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YDR379C-A(YDR379C-A)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YFR020W(YFR020W_p)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL177W(YGL177W_d)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR115W(YJR115W_p)|FD-Score:-3.13|P-value:8.76E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YLR224W(YLR224W_p)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YLR312C(YLR312C_p)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Putative protein of unknown function Gene:YMR130W(YMR130W_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YMR317W(YMR317W_p)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YOR139C(YOR139C_d)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL025C(YPL025C_d)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL080C(YPL080C_d)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL113C(YPL113C_p)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:ZRG8(YER033C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ZWF1(YNL241C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER006W4.072.33E-50.38NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YBR140C3.826.57E-50.38IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YOL040C3.729.98E-50.38RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YLR339C_d3.551.92E-40.38YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
YGL047W3.177.69E-40.01ALG13Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
YDL193W3.167.95E-40.05NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YDR243C3.109.56E-40.12PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YAL034C-B_d2.990.001400.05YAL034C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR208W2.940.001660.00SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YOR157C2.930.001680.00PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YBL092W2.930.001700.03RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YJL143W2.900.001850.03TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YLL035W2.870.002040.18GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
YOR020C2.690.003610.07HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YFR004W2.620.004430.10RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL197W6.114.89E-10UBP12Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm
YAL037W_p5.238.33E-8YAL037W_pPutative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication
YEL009C4.513.30E-6GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YNL016W4.405.31E-6PUB1Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress
YDR126W4.318.16E-6SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YLR224W_p4.171.54E-5YLR224W_pF-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene
YIR018W4.102.03E-5YAP5Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication
YLR092W4.082.29E-5SUL2High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
YNL241C4.072.34E-5ZWF1Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress
YBL104C3.973.55E-5SEA4Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites
YIL038C3.895.11E-5NOT3Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
YJR094W-A3.846.08E-5RPL43BRibosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YBR291C3.787.91E-5CTP1Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family
YPL021W3.711.04E-4ECM23Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
YJL030W3.711.05E-4MAD2Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I

GO enrichment analysis for SGTC_3061
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0921.51E-12SGTC_340469-0683 81.8 μMChemDiv (Drug-like library)36909230.0178571SWF1 & branched chain AA biosynthesis
0.0864.89E-11SGTC_20345197015 53.1 μMChembridge (Fragment library)7806760.0363636SWF1 & branched chain AA biosynthesis
0.0773.53E-9SGTC_1524469-0003 551.2 μMChemDiv (Drug-like library)6972800.0192308
0.0774.40E-9SGTC_6164092-0450 108.0 μMChemDiv (Drug-like library)7871650.037037
0.0716.32E-8SGTC_10410206-0046 107.0 μMChemDiv (Drug-like library)504940.0363636
0.0691.42E-7SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0212766heme biosynthesis & mitochondrial translocase
0.0663.60E-7SGTC_2851486-1325 46.0 μMChemDiv (Drug-like library)223102300.0163934
0.0648.33E-7SGTC_11604092-0570 10.5 μMChemDiv (Drug-like library)49949750.031746plasma membrane duress
0.0631.30E-6SGTC_6360kpi-0074 126.0 μMChemDiv (Drug-like library)6164340.0212766
0.0631.37E-6SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.0892857SWF1 & branched chain AA biosynthesis
0.0631.58E-6SGTC_18825615643 20.0 μMMiscellaneous22530750.0441176TRP & mitochondrial translation
0.0622.32E-6SGTC_13873342-1166 1.8 μMChemDiv (Drug-like library)34182280.0188679mitochondrial processes
0.0612.79E-6SGTC_2717st081029 62.3 μMTimTec (Natural product derivative library)52928460.0634921
0.0612.79E-6SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.0172414
0.0613.45E-6SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0652174

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_474dl-dihydrosphingosine5 μM0.2571433058739ICCB bioactive library301.507765.13433
SGTC_438sphingosine6.67 μM0.2255280335ICCB bioactive library299.491884.82433
SGTC_2524avocatin b10.57 μM0.1794876710748Microsource (Natural product library)570.884248.94166
SGTC_2585alliin100 μM0.17587310Microsource (Natural product library)177.22144-3.39125
SGTC_1876myriocin605 nM0.163636301119Miscellaneous401.537460.28457
SGTC_436n,n-dimethylsphingosine3.3 μM0.1590915282309ICCB bioactive library327.545045.79223
SGTC_1796st049173110.4 μM0.15384694134TimTec (Natural product derivative library)181.18854-2.43734
SGTC_2618avocadyne43.43 μM0.153015189Microsource (Natural product library)284.434184.75833
SGTC_4732-arachidonoylglycerol10 μM0.1458335282280ICCB bioactive library378.545425.61424
SGTC_1564D-Arginine115 μM0.14285771070TimTec (Pure natural product library)174.20096-3.83144