1327-0027

N-benzyl-4-[bromo(dichloro)methyl]sulfonyl-2-nitroaniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3064
Screen concentration 10.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 16000455
SMILES C1=CC=C(C=C1)CNC2=C(C=C(C=C2)S(=O)(=O)C(Cl)(Cl)Br)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1cc(ccc1NCc2ccccc2)S(=O)(=O)C(Cl)(Cl)Br
Molecular weight 454.1231
ALogP 5.75
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.47
% growth inhibition (Hom. pool) 13.03


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16000455
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.21||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:GPI14(YJR013W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.15||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:GPI17(YDR434W)|FD-Score:3.84|P-value:6.11E-5|Clearance:0.44||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:MIF2(YKL089W)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.16||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:MTW1(YAL034W-A)|FD-Score:4.03|P-value:2.81E-5|Clearance:0.19||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NMD3(YHR170W)|FD-Score:-3.78|P-value:7.79E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RPB3(YIL021W)|FD-Score:5.16|P-value:1.24E-7|Clearance:0.92||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL10(YLR075W)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RSC58(YLR033W)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SPC105(YGL093W)|FD-Score:12.4|P-value:7.36E-36|Clearance:7.29||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TAF3(YPL011C)|FD-Score:3.26|P-value:5.65E-4|Clearance:0.11||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:YTH1(YPR107C)|FD-Score:27.3|P-value:2.26E-164|Clearance:14.85||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.21||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:GPI14(YJR013W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.15||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:GPI17(YDR434W)|FD-Score:3.84|P-value:6.11E-5|Clearance:0.44||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:MIF2(YKL089W)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.16||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:MTW1(YAL034W-A)|FD-Score:4.03|P-value:2.81E-5|Clearance:0.19||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NMD3(YHR170W)|FD-Score:-3.78|P-value:7.79E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RPB3(YIL021W)|FD-Score:5.16|P-value:1.24E-7|Clearance:0.92||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL10(YLR075W)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RSC58(YLR033W)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SPC105(YGL093W)|FD-Score:12.4|P-value:7.36E-36|Clearance:7.29||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TAF3(YPL011C)|FD-Score:3.26|P-value:5.65E-4|Clearance:0.11||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:YTH1(YPR107C)|FD-Score:27.3|P-value:2.26E-164|Clearance:14.85||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16000455
Download HOP data (tab-delimited text)  (excel)
Gene:ARO1(YDR127W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG11(YPR049C)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATH1(YPR026W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:BCK1(YJL095W)|FD-Score:7.21|P-value:2.70E-13||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CNB1(YKL190W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COA2(YPL189C-A)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:FYV10(YIL097W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:IMP2'(YIL154C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRE1(YHR079C)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:MRPL35(YDR322W)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NHX1(YDR456W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:PDR1(YGL013C)|FD-Score:11.2|P-value:1.96E-29||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:SLT2(YHR030C)|FD-Score:7.85|P-value:2.16E-15||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOD2(YHR008C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPF1(YEL031W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT3(YDR392W)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:THR1(YHR025W)|FD-Score:5.48|P-value:2.17E-8||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRP4(YDR354W)|FD-Score:9.13|P-value:3.55E-20||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.08|P-value:5.19E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YEN1(YER041W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFH7(YFR007W)|FD-Score:6.42|P-value:6.73E-11||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGR069W(YGR069W_d)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function Gene:YPL080C(YPL080C_d)|FD-Score:-4.84|P-value:6.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:ARO1(YDR127W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG11(YPR049C)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATH1(YPR026W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:BCK1(YJL095W)|FD-Score:7.21|P-value:2.70E-13||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CNB1(YKL190W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COA2(YPL189C-A)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:FYV10(YIL097W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:IMP2'(YIL154C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRE1(YHR079C)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:MRPL35(YDR322W)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NHX1(YDR456W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:PDR1(YGL013C)|FD-Score:11.2|P-value:1.96E-29||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:SLT2(YHR030C)|FD-Score:7.85|P-value:2.16E-15||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOD2(YHR008C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPF1(YEL031W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT3(YDR392W)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:THR1(YHR025W)|FD-Score:5.48|P-value:2.17E-8||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRP4(YDR354W)|FD-Score:9.13|P-value:3.55E-20||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.08|P-value:5.19E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YEN1(YER041W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YFH7(YFR007W)|FD-Score:6.42|P-value:6.73E-11||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGR069W(YGR069W_d)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function Gene:YPL080C(YPL080C_d)|FD-Score:-4.84|P-value:6.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C27.302.26E-16414.90YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YGL093W12.407.36E-367.29SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YIL021W5.161.24E-70.92RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YNR016C4.241.14E-50.21ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YAL034W-A4.032.81E-50.19MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YDR434W3.846.11E-50.44GPI17Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
YJR013W3.403.33E-40.15GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YPL011C3.265.65E-40.11TAF3TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
YLR033W3.158.29E-40.01RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YKL089W3.138.70E-40.16MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
YDR353W2.970.001500.09TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
YDL060W2.880.002000.03TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YPR186C2.850.002190.11PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YGL247W2.740.003090.02BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YDR118W2.720.003260.08APC4Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C11.201.96E-29PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YDR354W9.133.55E-20TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YBR058C-A9.085.19E-20TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YHR030C7.852.16E-15SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YJL095W7.212.70E-13BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YFR007W6.426.73E-11YFH7Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases
YHR025W5.482.17E-8THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YHR079C5.081.93E-7IRE1Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
YDR392W3.973.58E-5SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YPL189C-A3.836.48E-5COA2Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p
YCR053W3.749.38E-5THR4Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
YGR069W_d3.729.83E-5YGR069W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL190W3.691.10E-4CNB1Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress
YIL154C3.671.21E-4IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YEL031W3.522.19E-4SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1

GO enrichment analysis for SGTC_3064
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.7700SGTC_30601327-0027 12.5 μMChemDiv (Drug-like library)160004551
0.6880SGTC_30631327-0034 4.0 μMChemDiv (Drug-like library)159985800.563636
0.6780SGTC_30591327-0034 3.0 μMChemDiv (Drug-like library)159985800.563636
0.4569.47E-299SGTC_24295902844 64.4 μMMiscellaneous19277900.0694444
0.3561.78E-174SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.0833333SWF1 & branched chain AA biosynthesis
0.3544.77E-173SGTC_216k038-0035 28.4 μMChemDiv (Drug-like library)44442310.0405405ubiquinone biosynthesis & proteasome
0.3523.33E-170SGTC_5860957-0265 8.2 μMChemDiv (Drug-like library)67523090.0752688cell wall
0.3377.69E-156SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0724638
0.3282.45E-147SGTC_30229087993 49.5 μMChembridge (Drug-like library)171736440.0722892
0.3142.16E-134SGTC_1420335-0849 62.7 μMChemDiv (Drug-like library)38890430.146667ubiquinone biosynthesis & proteasome
0.3062.63E-127SGTC_200k038-0079 37.5 μMChemDiv (Drug-like library)34366830.0519481ubiquinone biosynthesis & proteasome
0.3038.55E-125SGTC_1968st069590 43.6 μMTimTec (Natural product derivative library)63840660.0945946
0.2981.09E-120SGTC_294k038-0071 157.0 μMChemDiv (Drug-like library)241528260.0384615ubiquinone biosynthesis & proteasome
0.2942.04E-117SGTC_1370438-0332 85.4 μMChemDiv (Drug-like library)20633930.047619ubiquinone biosynthesis & proteasome
0.2855.02E-110SGTC_3324112-0506 40.9 μMChemDiv (Drug-like library)27903550.025641

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_30601327-002712.5 μM116000455ChemDiv (Drug-like library)454.123145.74815
SGTC_1631327-00367.55 μM0.57407415992207ChemDiv (Drug-like library)432.117625.56615
SGTC_26511327-00365 μM0.57407415992207ChemDiv (Drug-like library)432.117625.56615
SGTC_30591327-00343 μM0.56363615998580ChemDiv (Drug-like library)446.14426.02315
SGTC_30631327-00344 μM0.56363615998580ChemDiv (Drug-like library)446.14426.02315
SGTC_797k781-0471270 μM0.43103423738129ChemDiv (Drug-like library)306.701143.4125RPP1 & pyrimidine depletion
SGTC_225nsc-1226571.02 μM0.350877275685Miscellaneous322.29332.72806
SGTC_6520437-0092114 μM0.3289473091274ChemDiv (Drug-like library)507.775385.60636
SGTC_2816798270658.44 μM0.3235292981853Chembridge (Drug-like library)326.392883.50115azole & statin
SGTC_260302-0163425.16 μM0.3103453100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_458nppb133 μM0.3076924549ICCB bioactive library300.309243.52325
SGTC_1764st04450293.5 μM0.3076928407TimTec (Natural product derivative library)214.223.27413