9117431

4-methoxy-N-(2-propan-2-yloxyphenyl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3067
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17150885
SMILES CC(C)OC1=CC=CC=C1NC(=O)C2=CC=C(C=C2)OC
Standardized SMILES COc1ccc(cc1)C(=O)Nc2ccccc2OC(C)C
Molecular weight 285.3377
ALogP 3.31
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.79
% growth inhibition (Hom. pool) 5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17150885
Download HIP data (tab-delimited text)  (excel)
Gene:CAB3(YKL088W)|FD-Score:3.84|P-value:6.03E-5|Clearance:0.19||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CAK1(YFL029C)|FD-Score:4.42|P-value:4.95E-6|Clearance:0.13||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCL1(YPR025C)|FD-Score:3.88|P-value:5.15E-5|Clearance:0.04||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CFT1(YDR301W)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.01||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:ERD2(YBL040C)|FD-Score:3.96|P-value:3.74E-5|Clearance:0.08||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG13(YML126C)|FD-Score:-3.13|P-value:8.65E-4|Clearance:0||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:FOL3(YMR113W)|FD-Score:-3.78|P-value:7.97E-5|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:IRA1(YBR140C)|FD-Score:-4.64|P-value:1.78E-6|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MED4(YOR174W)|FD-Score:3.16|P-value:7.88E-4|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MOT1(YPL082C)|FD-Score:4.29|P-value:8.86E-6|Clearance:0.13||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NAT2(YGR147C)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.32||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NUG1(YER006W)|FD-Score:3.19|P-value:7.17E-4|Clearance:0.03||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PFS2(YNL317W)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.11||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PSF1(YDR013W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.13||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RRN3(YKL125W)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.08||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SEN34(YAR008W)|FD-Score:3.28|P-value:5.12E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SSC1(YJR045C)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.06||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TAF1(YGR274C)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.08||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TAF9(YMR236W)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.09||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TIM23(YNR017W)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.06||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TSR4(YOL022C)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.03||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:WRS1(YOL097C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.05||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGR073C(YGR073C_d)|FD-Score:4.74|P-value:1.09E-6|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:CAB3(YKL088W)|FD-Score:3.84|P-value:6.03E-5|Clearance:0.19||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CAK1(YFL029C)|FD-Score:4.42|P-value:4.95E-6|Clearance:0.13||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCL1(YPR025C)|FD-Score:3.88|P-value:5.15E-5|Clearance:0.04||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CFT1(YDR301W)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.01||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:ERD2(YBL040C)|FD-Score:3.96|P-value:3.74E-5|Clearance:0.08||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG13(YML126C)|FD-Score:-3.13|P-value:8.65E-4|Clearance:0||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:FOL3(YMR113W)|FD-Score:-3.78|P-value:7.97E-5|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:IRA1(YBR140C)|FD-Score:-4.64|P-value:1.78E-6|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MED4(YOR174W)|FD-Score:3.16|P-value:7.88E-4|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MOT1(YPL082C)|FD-Score:4.29|P-value:8.86E-6|Clearance:0.13||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NAT2(YGR147C)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.32||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NUG1(YER006W)|FD-Score:3.19|P-value:7.17E-4|Clearance:0.03||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PFS2(YNL317W)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.11||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PSF1(YDR013W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.13||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RRN3(YKL125W)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.08||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SEN34(YAR008W)|FD-Score:3.28|P-value:5.12E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SSC1(YJR045C)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.06||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TAF1(YGR274C)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.08||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:TAF9(YMR236W)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.09||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TIM23(YNR017W)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.06||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TSR4(YOL022C)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.03||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:WRS1(YOL097C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.05||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGR073C(YGR073C_d)|FD-Score:4.74|P-value:1.09E-6|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17150885
Download HOP data (tab-delimited text)  (excel)
Gene:ARD1(YHR013C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ATG5(YPL149W)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BAS1(YKR099W)|FD-Score:-3.79|P-value:7.66E-5||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BSP1(YPR171W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CHL1(YPL008W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:DDI3(YNL335W_p)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DSE2(YHR143W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ERG5(YMR015C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:HHF1(YBR009C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIS6(YIL020C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HXT4(YHR092C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:IRA2(YOL081W)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRC10(YOL015W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC21(YMR073C)|FD-Score:-4.28|P-value:9.37E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC25(YLR021W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:KAP114(YGL241W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KSS1(YGR040W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:MDE1(YJR024C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant Gene:MGR3(YMR115W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MNT4(YNR059W)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRI1(YPR118W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRM1(YOR201C)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MSC2(YDR205W)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MTM1(YGR257C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NIT2(YJL126W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NPR2(YEL062W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NTG1(YAL015C)|FD-Score:-3.54|P-value:1.96E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:PES4(YFR023W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PRS1(YKL181W)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RHR2(YIL053W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RSM27(YGR215W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SGN1(YIR001C)|FD-Score:4.42|P-value:4.99E-6||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SMA1(YPL027W)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SOD2(YHR008C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SVL3(YPL032C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TOS6(YNL300W_p)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPP1(YMR156C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP1(YDR007W)|FD-Score:4.51|P-value:3.20E-6||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS33(YOR115C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:VMA16(YHR026W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA9(YCL005W-A)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS73(YGL104C)|FD-Score:4.39|P-value:5.61E-6||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YAT2(YER024W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane Gene:YDR336W(YDR336W_p)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YER039C-A(YER039C-A_p)|FD-Score:-4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YIL166C(YIL166C_p)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJL175W(YJL175W_d)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YJR003C(YJR003C_p)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL031W(YKL031W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YNL211C(YNL211C_p)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YNL276C(YNL276C_d)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YPR089W(YPR089W_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR126C(YPR126C_d)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR195C(YPR195C_d)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YSC83(YHR017W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:ARD1(YHR013C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ATG5(YPL149W)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BAS1(YKR099W)|FD-Score:-3.79|P-value:7.66E-5||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BSP1(YPR171W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CHL1(YPL008W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:DDI3(YNL335W_p)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DSE2(YHR143W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ERG5(YMR015C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:HHF1(YBR009C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIS6(YIL020C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HXT4(YHR092C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:IRA2(YOL081W)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRC10(YOL015W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC21(YMR073C)|FD-Score:-4.28|P-value:9.37E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC25(YLR021W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:KAP114(YGL241W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KSS1(YGR040W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:MDE1(YJR024C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant Gene:MGR3(YMR115W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MNT4(YNR059W)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRI1(YPR118W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRM1(YOR201C)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MSC2(YDR205W)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MTM1(YGR257C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NIT2(YJL126W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NPR2(YEL062W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NTG1(YAL015C)|FD-Score:-3.54|P-value:1.96E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:PES4(YFR023W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PRS1(YKL181W)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RHR2(YIL053W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RSM27(YGR215W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SGN1(YIR001C)|FD-Score:4.42|P-value:4.99E-6||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SMA1(YPL027W)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SOD2(YHR008C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SVL3(YPL032C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TOS6(YNL300W_p)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPP1(YMR156C)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP1(YDR007W)|FD-Score:4.51|P-value:3.20E-6||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS33(YOR115C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:VMA16(YHR026W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA9(YCL005W-A)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS73(YGL104C)|FD-Score:4.39|P-value:5.61E-6||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YAT2(YER024W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane Gene:YDR336W(YDR336W_p)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YER039C-A(YER039C-A_p)|FD-Score:-4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YIL166C(YIL166C_p)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJL175W(YJL175W_d)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YJR003C(YJR003C_p)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL031W(YKL031W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YNL211C(YNL211C_p)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YNL276C(YNL276C_d)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YPR089W(YPR089W_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR126C(YPR126C_d)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR195C(YPR195C_d)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YSC83(YHR017W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR147C4.751.03E-60.32NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YGR073C_d4.741.09E-60.32YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YFL029C4.424.95E-60.13CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YPL082C4.298.86E-60.13MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YOL022C4.161.60E-50.03TSR4Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
YMR236W4.131.85E-50.09TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YGR274C4.042.71E-50.08TAF1TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression
YBL040C3.963.74E-50.08ERD2HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
YPR025C3.885.15E-50.04CCL1Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
YKL088W3.846.03E-50.20CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YDR013W3.651.31E-40.13PSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YJR045C3.522.13E-40.06SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YNL317W3.462.72E-40.12PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YNR017W3.344.14E-40.06TIM23Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
YAR008W3.285.12E-40.00SEN34Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL181W4.582.30E-6PRS15-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
YDR007W4.513.20E-6TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YIR001C4.424.99E-6SGN1Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation
YGL104C4.395.61E-6VPS73Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family
YHR026W4.131.81E-5VMA16Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
YER024W4.131.81E-5YAT2Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
YGL241W4.092.17E-5KAP114Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress
YMR156C3.944.12E-5TPP1DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase
YPL149W3.914.55E-5ATG5Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation
YFR031C-A3.904.76E-5RPL2ARibosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
YHR143W3.904.82E-5DSE2Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
YDR205W3.885.15E-5MSC2Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
YPL008W3.846.27E-5CHL1Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome
YOR201C3.836.30E-5MRM1Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
YNL211C_p3.787.82E-5YNL211C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene

GO enrichment analysis for SGTC_3067
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0613.27E-6SGTC_31479097027 49.5 μMChembridge (Drug-like library)252368410.136364
0.0603.53E-6SGTC_2702st077768 96.8 μMTimTec (Natural product derivative library)64841190.0625
0.0604.63E-6SGTC_1505k297-0037 20.6 μMChemDiv (Drug-like library)40893960.0895522
0.0595.34E-6SGTC_12140358-0031 63.7 μMChemDiv (Drug-like library)43013660.0895522
0.0596.13E-6SGTC_9791426-2828 121.0 μMChemDiv (Drug-like library)7478720.2
0.0586.98E-6SGTC_10064099-6547 255.0 μMChemDiv (Drug-like library)31468330.0735294
0.0588.30E-6SGTC_30709117005 49.5 μMChembridge (Drug-like library)87790160.377358
0.0589.01E-6SGTC_29989066762 71.4 μMChembridge (Drug-like library)49451960.480769RPP1 & pyrimidine depletion
0.0552.22E-5SGTC_1605st000082 49.1 μMTimTec (Natural product derivative library)11140220.12162260S ribosome export
0.0552.32E-5SGTC_14364092-1007 8.3 μMChemDiv (Drug-like library)28811210.0724638
0.0543.23E-5SGTC_20795212404 121.0 μMChembridge (Fragment library)7584620.0338983
0.0533.99E-5SGTC_30749116018 49.5 μMChembridge (Drug-like library)170273030.261538RNA processing & uracil transport
0.0526.84E-5SGTC_217ketoconazole 989.0 nMMiscellaneous4562010.104167ergosterol depletion effects on membrane
0.0519.65E-5SGTC_30519093778 49.5 μMChembridge (Drug-like library)172173780.212121
0.0501.15E-4SGTC_19225578187 25.0 μMMiscellaneous13504120.126761TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3077911731949.47 μM0.58695717154678Chembridge (Drug-like library)285.337663.30813
SGTC_9383126-1755342 μM0.5652171777771ChemDiv (Drug-like library)390.388723.01235
SGTC_2999906976571.43 μM0.5531912097066Chembridge (Drug-like library)285.337663.30813amide catabolism
SGTC_2877904072471.43 μM0.5208336463895Chembridge (Drug-like library)285.337663.30813
SGTC_3250913558349.47 μM0.57699353Chembridge (Drug-like library)299.321182.80214
SGTC_2998906676271.43 μM0.4807694945196Chembridge (Drug-like library)297.348363.54713RPP1 & pyrimidine depletion
SGTC_8261683-5028118 μM0.479167722377ChemDiv (Drug-like library)261.703583.26212
SGTC_9363005-4620401 μM0.471698777207ChemDiv (Drug-like library)295.296021.48515
SGTC_2986902244571.43 μM0.466459286Chembridge (Drug-like library)285.337663.30813
SGTC_3195911308249.47 μM0.4489817018038Chembridge (Drug-like library)301.337063.05114