9116768

3-butyl-6-(2-phenylethyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3068
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 41904888
SMILES CCCCC1=NN=C2N1N=C(S2)CCC3=CC=CC=C3
Standardized SMILES CCCCc1nnc2sc(CCc3ccccc3)nn12
Molecular weight 286.3952
ALogP 3.78
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.63
% growth inhibition (Hom. pool) 5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 41904888
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.18|P-value:7.37E-4|Clearance:0.26||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT8(YJL008C)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC39(YCR093W)|FD-Score:4.9|P-value:4.81E-7|Clearance:0.3||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DPB11(YJL090C)|FD-Score:-4.02|P-value:2.87E-5|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DSL1(YNL258C)|FD-Score:-3.32|P-value:4.47E-4|Clearance:0||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:ERG12(YMR208W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.12||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI12(YMR281W)|FD-Score:3.19|P-value:7.03E-4|Clearance:0.01||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:NMD3(YHR170W)|FD-Score:-3.88|P-value:5.17E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP15(YNL110C)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PRP43(YGL120C)|FD-Score:4.6|P-value:2.10E-6|Clearance:0.53||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RPB10(YOR210W)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.15||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPS15(YOL040C)|FD-Score:5.16|P-value:1.24E-7|Clearance:0.26||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS31(YLR167W)|FD-Score:4.07|P-value:2.31E-5|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RSC58(YLR033W)|FD-Score:-6.22|P-value:2.42E-10|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RVB2(YPL235W)|FD-Score:-6.45|P-value:5.68E-11|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SAR1(YPL218W)|FD-Score:4.07|P-value:2.35E-5|Clearance:0.18||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SEC31(YDL195W)|FD-Score:6.12|P-value:4.55E-10|Clearance:0.97||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMC4(YLR086W)|FD-Score:-3.25|P-value:5.74E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SPB4(YFL002C)|FD-Score:-4.57|P-value:2.39E-6|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFC6(YDR362C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.13||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:VRG4(YGL225W)|FD-Score:3.89|P-value:5.00E-5|Clearance:0.04||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YGR114C(YGR114C_d)|FD-Score:3.85|P-value:5.87E-5|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL009W(YJL009W_d)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YPR142C(YPR142C_d)|FD-Score:-4.22|P-value:1.20E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:ACT1(YFL039C)|FD-Score:3.18|P-value:7.37E-4|Clearance:0.26||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT8(YJL008C)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC39(YCR093W)|FD-Score:4.9|P-value:4.81E-7|Clearance:0.3||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DPB11(YJL090C)|FD-Score:-4.02|P-value:2.87E-5|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DSL1(YNL258C)|FD-Score:-3.32|P-value:4.47E-4|Clearance:0||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:ERG12(YMR208W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.12||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI12(YMR281W)|FD-Score:3.19|P-value:7.03E-4|Clearance:0.01||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:NMD3(YHR170W)|FD-Score:-3.88|P-value:5.17E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP15(YNL110C)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PRP43(YGL120C)|FD-Score:4.6|P-value:2.10E-6|Clearance:0.53||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RPB10(YOR210W)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.15||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPS15(YOL040C)|FD-Score:5.16|P-value:1.24E-7|Clearance:0.26||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS31(YLR167W)|FD-Score:4.07|P-value:2.31E-5|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RSC58(YLR033W)|FD-Score:-6.22|P-value:2.42E-10|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RVB2(YPL235W)|FD-Score:-6.45|P-value:5.68E-11|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SAR1(YPL218W)|FD-Score:4.07|P-value:2.35E-5|Clearance:0.18||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SEC31(YDL195W)|FD-Score:6.12|P-value:4.55E-10|Clearance:0.97||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMC4(YLR086W)|FD-Score:-3.25|P-value:5.74E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SPB4(YFL002C)|FD-Score:-4.57|P-value:2.39E-6|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFC6(YDR362C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.13||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:VRG4(YGL225W)|FD-Score:3.89|P-value:5.00E-5|Clearance:0.04||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YGR114C(YGR114C_d)|FD-Score:3.85|P-value:5.87E-5|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL009W(YJL009W_d)|FD-Score:-3.57|P-value:1.81E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YPR142C(YPR142C_d)|FD-Score:-4.22|P-value:1.20E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 41904888
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:6.48|P-value:4.44E-11||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AGE2(YIL044C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIM9(YER080W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALT1(YLR089C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:APL6(YGR261C)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM1(YPL259C)|FD-Score:5.42|P-value:2.99E-8||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ATG23(YLR431C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:AVT1(YJR001W)|FD-Score:-3.1|P-value:9.63E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BDF1(YLR399C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:COQ5(YML110C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTF19(YPL018W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CTF8(YHR191C)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:ECM11(YDR446W)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure Gene:FIG4(YNL325C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FMO1(YHR176W)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GGC1(YDL198C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GIT1(YCR098C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GYP1(YOR070C)|FD-Score:6.2|P-value:2.90E-10||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HPA2(YPR193C)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HSL7(YBR133C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IRC9(YJL142C_d)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LAT1(YNL071W)|FD-Score:-3.8|P-value:7.26E-5||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LSC2(YGR244C)|FD-Score:4.95|P-value:3.65E-7||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAL13(YGR288W)|FD-Score:-4.61|P-value:2.06E-6||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAL31(YBR298C)|FD-Score:8.09|P-value:3.00E-16||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MET10(YFR030W)|FD-Score:5.76|P-value:4.08E-9||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MF(ALPHA)2(YGL089C)|FD-Score:-3.13|P-value:8.61E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 Gene:MPC2(YHR162W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MPD2(YOL088C)|FD-Score:4.94|P-value:3.96E-7||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPS16(YPL013C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSA2(YKR077W)|FD-Score:-9.63|P-value:3.09E-22||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSH2(YOL090W)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NAT1(YDL040C)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:ORM1(YGR038W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAN6(YIL145C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC Gene:PCD1(YLR151C)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PES4(YFR023W)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET54(YGR222W)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PIB2(YGL023C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PTC3(YBL056W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:REC104(YHR157W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RIT1(YMR283C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPS18A(YDR450W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:SDS23(YGL056C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SET5(YHR207C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SHM2(YLR058C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SPR3(YGR059W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SPT2(YER161C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSA3(YBL075C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SUV3(YPL029W)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:THI74(YDR438W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP4(YDR354W)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VMA7(YGR020C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAP1801(YHR161C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR056W(YBR056W_p)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR226C(YBR226C_d)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCR064C(YCR064C_d)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL032W(YDL032W_d)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR124W(YDR124W_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR306C(YDR306C_p)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YER134C(YER134C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGL010W(YGL010W_p)|FD-Score:4.8|P-value:7.91E-7||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR151C(YGR151C_d)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-4.12|P-value:1.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR097C(YHR097C_p)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YLR334C(YLR334C_d)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR446W(YLR446W_p)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YMR084W(YMR084W_p)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR063W(YNR063W_p)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR015W(YOR015W_d)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR022C(YOR022C_p)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL039W(YPL039W_p)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YSP3(YOR003W)|FD-Score:-5|P-value:2.86E-7||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:YUR1(YJL139C)|FD-Score:4.86|P-value:5.82E-7||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:YVH1(YIR026C)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ACE2(YLR131C)|FD-Score:6.48|P-value:4.44E-11||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AGE2(YIL044C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIM9(YER080W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALT1(YLR089C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:APL6(YGR261C)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM1(YPL259C)|FD-Score:5.42|P-value:2.99E-8||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ATG23(YLR431C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:AVT1(YJR001W)|FD-Score:-3.1|P-value:9.63E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BDF1(YLR399C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:COQ5(YML110C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTF19(YPL018W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CTF8(YHR191C)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:ECM11(YDR446W)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure Gene:FIG4(YNL325C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FMO1(YHR176W)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GGC1(YDL198C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GIT1(YCR098C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GYP1(YOR070C)|FD-Score:6.2|P-value:2.90E-10||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HPA2(YPR193C)|FD-Score:-3.16|P-value:7.86E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HSL7(YBR133C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IRC9(YJL142C_d)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LAT1(YNL071W)|FD-Score:-3.8|P-value:7.26E-5||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LSC2(YGR244C)|FD-Score:4.95|P-value:3.65E-7||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAL13(YGR288W)|FD-Score:-4.61|P-value:2.06E-6||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAL31(YBR298C)|FD-Score:8.09|P-value:3.00E-16||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MET10(YFR030W)|FD-Score:5.76|P-value:4.08E-9||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MF(ALPHA)2(YGL089C)|FD-Score:-3.13|P-value:8.61E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 Gene:MPC2(YHR162W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MPD2(YOL088C)|FD-Score:4.94|P-value:3.96E-7||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPS16(YPL013C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSA2(YKR077W)|FD-Score:-9.63|P-value:3.09E-22||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSH2(YOL090W)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NAT1(YDL040C)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:ORM1(YGR038W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAN6(YIL145C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC Gene:PCD1(YLR151C)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PES4(YFR023W)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET54(YGR222W)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PIB2(YGL023C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PTC3(YBL056W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:REC104(YHR157W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RIT1(YMR283C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPS18A(YDR450W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:SDS23(YGL056C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SET5(YHR207C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SHM2(YLR058C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SPR3(YGR059W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SPT2(YER161C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSA3(YBL075C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SUV3(YPL029W)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:THI74(YDR438W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP4(YDR354W)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VMA7(YGR020C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAP1801(YHR161C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR056W(YBR056W_p)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR226C(YBR226C_d)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCR064C(YCR064C_d)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL032W(YDL032W_d)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR124W(YDR124W_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR306C(YDR306C_p)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YER134C(YER134C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGL010W(YGL010W_p)|FD-Score:4.8|P-value:7.91E-7||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR151C(YGR151C_d)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-4.12|P-value:1.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR097C(YHR097C_p)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YLR334C(YLR334C_d)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR446W(YLR446W_p)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YMR084W(YMR084W_p)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR063W(YNR063W_p)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR015W(YOR015W_d)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR022C(YOR022C_p)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL039W(YPL039W_p)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YSP3(YOR003W)|FD-Score:-5|P-value:2.86E-7||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:YUR1(YJL139C)|FD-Score:4.86|P-value:5.82E-7||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:YVH1(YIR026C)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL195W6.124.55E-100.96SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YOL040C5.161.24E-70.26RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YCR093W4.904.81E-70.30CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YGL120C4.602.10E-60.53PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YLR167W4.072.31E-50.00RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YPL218W4.072.35E-50.18SAR1GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport
YGL225W3.895.00E-50.04VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YGR114C_d3.855.87E-50.25YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YDR362C3.601.61E-40.13TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YMR208W3.462.66E-40.12ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YOR210W3.344.18E-40.15RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YMR281W3.197.03E-40.01GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YFL039C3.187.37E-40.26ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YDR473C2.920.001740.00PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YDL153C2.920.001770.25SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR298C8.093.00E-16MAL31Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C
YLR131C6.484.44E-11ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YOR070C6.202.90E-10GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YFR030W5.764.08E-9MET10Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide
YPL259C5.422.99E-8APM1Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting
YDR354W5.091.77E-7TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YGR244C4.953.65E-7LSC2Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
YOL088C4.943.96E-7MPD2Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
YJL139C4.865.82E-7YUR1Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
YGL010W_p4.807.91E-7YGL010W_pPutative protein of unknown function; YGL010W is not an essential gene
YGR151C_d4.731.13E-6YGR151C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress
YDL032W_d4.503.34E-6YDL032W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene
YIL044C4.261.01E-5AGE2ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif
YLR089C4.221.22E-5ALT1Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive
YGL056C4.171.54E-5SDS23One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3068
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1292.24E-23SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.0481928
0.1151.09E-18SGTC_1260417-1624 672.5 μMChemDiv (Drug-like library)419120.0588235DNA intercalators
0.0961.46E-13SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.0625
0.0953.26E-13SGTC_3174495-0002 170.0 μMChemDiv (Drug-like library)159916180.114286
0.0912.28E-12SGTC_2733amorolfine 100.0 μMMiscellaneous542600.1plasma membrane duress
0.0912.88E-12SGTC_1100850-0284 111.6 μMChemDiv (Drug-like library)39062650.0759494
0.0904.13E-12SGTC_1104tacrolimus 29.8 μMNIH Clinical Collection235817960.032
0.0881.18E-11SGTC_21485546263 113.6 μMChembridge (Fragment library)7920970.112903
0.0855.44E-11SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.107692
0.0822.63E-10SGTC_20645220758 200.0 μMChembridge (Fragment library)7885180.0869565
0.0805.87E-10SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0972222
0.0791.44E-9SGTC_1955wogonin 32.4 μMTimTec (Natural product derivative library)52817030.101449
0.0791.49E-9SGTC_1090132-0036 33.7 μMChemDiv (Drug-like library)672740.0882353
0.0781.76E-9SGTC_6690108-0021 26.2 μMChemDiv (Drug-like library)172010.0740741fatty acid desaturase (OLE1)
0.0772.48E-9SGTC_2725haloperidol 53.2 μMMiscellaneous35590.0649351plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3069911700949.47 μM0.59183741904981Chembridge (Drug-like library)284.379343.7610460S ribosome export
SGTC_3307912688569.28 μM0.57142924824909Chembridge (Drug-like library)272.368643.77904
SGTC_3210912815749.47 μM0.517590849Chembridge (Drug-like library)296.346983.26405
SGTC_3101912053149.47 μM0.49090924824904Chembridge (Drug-like library)288.368043.27705
SGTC_3117912494549.47 μM0.49090917592065Chembridge (Drug-like library)311.361622.89706
SGTC_3116912494349.47 μM0.44444441905195Chembridge (Drug-like library)288.368042.92405
SGTC_3302912539516.35 μM0.37517599668Chembridge (Drug-like library)284.379343.55704
SGTC_3102912166949.47 μM0.35714341905072Chembridge (Drug-like library)272.368643.57504
SGTC_3292912082559.78 μM0.35087724824900Chembridge (Drug-like library)276.3325233.29405
SGTC_2797785909881.82 μM0.338983972681Chembridge (Drug-like library)342.802743.87805