9117009

3-butyl-6-[(E)-2-phenylethenyl]-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3069
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 41904981
SMILES CCCCC1=NN=C2N1N=C(S2)C=CC3=CC=CC=C3
Standardized SMILES CCCCc1nnc2sc(C=Cc3ccccc3)nn12
Molecular weight 284.3793
ALogP 3.76
H-bond donor count 0
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.53
% growth inhibition (Hom. pool) 7.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 41904981
Download HIP data (tab-delimited text)  (excel)
Gene:DSL1(YNL258C)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.19||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:EMG1(YLR186W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:LSG1(YGL099W)|FD-Score:4.82|P-value:7.22E-7|Clearance:1.08||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MTR2(YKL186C)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NMD3(YHR170W)|FD-Score:6.88|P-value:3.05E-12|Clearance:2.06||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PFS2(YNL317W)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.1||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:RFT1(YBL020W)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPS31(YLR167W)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SEC62(YPL094C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.15||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFH1(YLR321C)|FD-Score:-3.57|P-value:1.79E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SSC1(YJR045C)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.24||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TSC10(YBR265W)|FD-Score:-5.08|P-value:1.93E-7|Clearance:0||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:DSL1(YNL258C)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.19||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:EMG1(YLR186W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:LSG1(YGL099W)|FD-Score:4.82|P-value:7.22E-7|Clearance:1.08||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MTR2(YKL186C)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NMD3(YHR170W)|FD-Score:6.88|P-value:3.05E-12|Clearance:2.06||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PFS2(YNL317W)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.1||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:RFT1(YBL020W)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPS31(YLR167W)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SEC62(YPL094C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.15||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFH1(YLR321C)|FD-Score:-3.57|P-value:1.79E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SSC1(YJR045C)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.24||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TSC10(YBR265W)|FD-Score:-5.08|P-value:1.93E-7|Clearance:0||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 41904981
Download HOP data (tab-delimited text)  (excel)
Gene:ALD6(YPL061W)|FD-Score:5.91|P-value:1.71E-9||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:AST2(YER101C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:BAT2(YJR148W)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BUD23(YCR047C)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CIN1(YOR349W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COQ5(YML110C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:DOG2(YHR043C)|FD-Score:-3.75|P-value:8.97E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:EMC2(YJR088C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:FAR10(YLR238W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:GPT2(YKR067W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:MAL33(YBR297W)|FD-Score:5.48|P-value:2.17E-8||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MRP17(YKL003C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MRPL25(YGR076C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MSC2(YDR205W)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MTD1(YKR080W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:MUP1(YGR055W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NAT3(YPR131C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:OGG1(YML060W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PEX12(YMR026C)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PGD1(YGL025C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:POC4(YPL144W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:RPS19A(YOL121C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SKN7(YHR206W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SKP2(YNL311C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:STV1(YMR054W)|FD-Score:4.5|P-value:3.32E-6||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TGL3(YMR313C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:THI3(YDL080C)|FD-Score:-3.97|P-value:3.66E-5||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TSR3(YOR006C)|FD-Score:3.9|P-value:4.91E-5||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:VIK1(YPL253C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS52(YDR484W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YBL059W(YBL059W_p)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YCR006C(YCR006C_d)|FD-Score:-9.22|P-value:1.51E-20||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR039W(YJR039W_p)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR269C(YLR269C_d)|FD-Score:-4.37|P-value:6.35E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR345W(YLR345W_p)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YML116W-A(YML116W-A_d)|FD-Score:3.8|P-value:7.23E-5||SGD DESC:Putative protein of unknown function Gene:YMR31(YFR049W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL146C-A(YNL146C-A_p)|FD-Score:6.32|P-value:1.29E-10||SGD DESC:Putative protein of unknown function Gene:YPR015C(YPR015C_p)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:ALD6(YPL061W)|FD-Score:5.91|P-value:1.71E-9||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:AST2(YER101C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:BAT2(YJR148W)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BUD23(YCR047C)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CIN1(YOR349W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COQ5(YML110C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:DOG2(YHR043C)|FD-Score:-3.75|P-value:8.97E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:EMC2(YJR088C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:FAR10(YLR238W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:GPT2(YKR067W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:MAL33(YBR297W)|FD-Score:5.48|P-value:2.17E-8||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MRP17(YKL003C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MRPL25(YGR076C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MSC2(YDR205W)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MTD1(YKR080W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:MUP1(YGR055W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NAT3(YPR131C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:OGG1(YML060W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PEX12(YMR026C)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PGD1(YGL025C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:POC4(YPL144W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:RPS19A(YOL121C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SKN7(YHR206W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SKP2(YNL311C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:STV1(YMR054W)|FD-Score:4.5|P-value:3.32E-6||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TGL3(YMR313C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:THI3(YDL080C)|FD-Score:-3.97|P-value:3.66E-5||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TSR3(YOR006C)|FD-Score:3.9|P-value:4.91E-5||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:VIK1(YPL253C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS52(YDR484W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YBL059W(YBL059W_p)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YCR006C(YCR006C_d)|FD-Score:-9.22|P-value:1.51E-20||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR039W(YJR039W_p)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR269C(YLR269C_d)|FD-Score:-4.37|P-value:6.35E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR345W(YLR345W_p)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YML116W-A(YML116W-A_d)|FD-Score:3.8|P-value:7.23E-5||SGD DESC:Putative protein of unknown function Gene:YMR31(YFR049W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL146C-A(YNL146C-A_p)|FD-Score:6.32|P-value:1.29E-10||SGD DESC:Putative protein of unknown function Gene:YPR015C(YPR015C_p)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W6.883.05E-122.06NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGL099W4.827.22E-71.08LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YJR045C3.749.17E-50.24SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YNL258C3.502.35E-40.18DSL1Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
YNL317W3.314.63E-40.10PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YPL094C3.216.53E-40.15SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YDR381W3.070.001080.14YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YPL011C2.920.001730.10TAF3TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
YKL018W2.820.002390.02SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
YJL069C2.800.002520.16UTP18Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
YER093C2.640.004120.02TSC11Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
YDR041W2.620.004370.03RSM10Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
YLR196W2.590.004770.01PWP1Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
YPL237W2.580.004920.03SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YBR136W2.550.005310.07MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL146C-A_p6.321.29E-10YNL146C-A_pPutative protein of unknown function
YPL061W5.911.71E-9ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YBR297W5.482.17E-8MAL33MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C
YMR313C5.181.09E-7TGL3Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation
YJR039W_p5.151.30E-7YJR039W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR054W4.503.32E-6STV1Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole
YLR238W3.914.69E-5FAR10Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate
YOR006C3.904.91E-5TSR3Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YCR047C3.846.04E-5BUD23Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
YML116W-A_d3.807.23E-5YML116W-A_dPutative protein of unknown function
YKL003C3.641.39E-4MRP17Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator
YER101C3.591.64E-4AST2Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
YPL253C3.561.88E-4VIK1Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p
YPR131C3.492.45E-4NAT3Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
YHR206W3.482.47E-4SKN7Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3069
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1943.20E-51SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.085714360S ribosome export
0.1902.76E-49SGTC_30739114972 49.5 μMChembridge (Drug-like library)419050750.31147560S ribosome export
0.1476.72E-30SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.12560S ribosome export
0.1461.58E-29SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.14705960S ribosome export
0.1352.47E-25SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.076923160S ribosome export
0.1241.17E-21SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.13888960S ribosome export
0.1202.03E-20SGTC_850868-0259 160.1 μMChemDiv (Drug-like library)831730.10769260S ribosome export
0.1181.03E-19SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.10465160S ribosome export
0.1164.27E-19SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.089552260S ribosome export
0.1156.30E-19SGTC_5794491-1009 110.0 μMChemDiv (Drug-like library)12710410.12121260S ribosome export
0.1156.59E-19SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.102941fatty acid desaturase (OLE1)
0.1151.10E-18SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.058823560S ribosome export
0.1132.44E-18SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.088607660S ribosome export
0.1093.72E-17SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.18181860S ribosome export
0.1054.36E-16SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.093333360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3302912539516.35 μM0.68085117599668Chembridge (Drug-like library)284.379343.55704
SGTC_3068911676849.47 μM0.59183741904888Chembridge (Drug-like library)286.395223.78404
SGTC_3307912688569.28 μM0.5490224824909Chembridge (Drug-like library)272.368643.77904
SGTC_3101912053149.47 μM0.47368424824904Chembridge (Drug-like library)288.368043.27705
SGTC_3210912815749.47 μM0.3793117590849Chembridge (Drug-like library)296.346983.26405
SGTC_3117912494549.47 μM0.37704917592065Chembridge (Drug-like library)311.361622.89706
SGTC_3116912494349.47 μM0.35593241905195Chembridge (Drug-like library)288.368042.92405
SGTC_3102912166949.47 μM0.34482841905072Chembridge (Drug-like library)272.368643.57504
SGTC_3292912082559.78 μM0.33898324824900Chembridge (Drug-like library)276.3325233.29405
SGTC_2996906619271.43 μM0.3278696472854Chembridge (Drug-like library)326.3481433.41906