1501-1223

N-[1-(4-bromoanilino)-2,2,2-trichloroethyl]furan-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_307
Screen concentration 22.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 2830329
SMILES C1=COC(=C1)C(=O)NC(C(Cl)(Cl)Cl)NC2=CC=C(C=C2)Br
Standardized SMILES ClC(Cl)(Cl)C(NC(=O)c1occc1)Nc2ccc(Br)cc2
Molecular weight 412.4937
ALogP 4.44
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.7
% growth inhibition (Hom. pool) 4.49


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2830329
Download HIP data (tab-delimited text)  (excel)
Gene:MRD1(YPR112C)|FD-Score:3.83|P-value:6.32E-5|Clearance:0.05||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:PRE6(YOL038W)|FD-Score:4.52|P-value:3.10E-6|Clearance:0.03||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP5(YBR237W)|FD-Score:4.01|P-value:3.09E-5|Clearance:0.17||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:SEC1(YDR164C)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC13(YLR208W)|FD-Score:4.49|P-value:3.55E-6|Clearance:0.49||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC39(YLR440C)|FD-Score:-3.16|P-value:7.82E-4|Clearance:0||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SKP1(YDR328C)|FD-Score:4.6|P-value:2.16E-6|Clearance:0.08||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SMC3(YJL074C)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:TFC8(YPL007C)|FD-Score:5.71|P-value:5.78E-9|Clearance:1.11||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:YGL069C(YGL069C_d)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.78|P-value:7.83E-5|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:MRD1(YPR112C)|FD-Score:3.83|P-value:6.32E-5|Clearance:0.05||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:PRE6(YOL038W)|FD-Score:4.52|P-value:3.10E-6|Clearance:0.03||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP5(YBR237W)|FD-Score:4.01|P-value:3.09E-5|Clearance:0.17||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:SEC1(YDR164C)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC13(YLR208W)|FD-Score:4.49|P-value:3.55E-6|Clearance:0.49||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC39(YLR440C)|FD-Score:-3.16|P-value:7.82E-4|Clearance:0||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SKP1(YDR328C)|FD-Score:4.6|P-value:2.16E-6|Clearance:0.08||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SMC3(YJL074C)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:TFC8(YPL007C)|FD-Score:5.71|P-value:5.78E-9|Clearance:1.11||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:YGL069C(YGL069C_d)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.78|P-value:7.83E-5|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2830329
Download HOP data (tab-delimited text)  (excel)
Gene:APP1(YNL094W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARG81(YML099C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ATG29(YPL166W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATG3(YNR007C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:BTS1(YPL069C)|FD-Score:-4.86|P-value:5.93E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CRP1(YHR146W)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Protein that binds to cruciform DNA structures Gene:CUS2(YNL286W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DMA2(YNL116W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DPH1(YIL103W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ERV46(YAL042W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:IRA2(YOL081W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:-4.5|P-value:3.32E-6||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LYS9(YNR050C)|FD-Score:4.27|P-value:9.64E-6||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MDM20(YOL076W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MEK1(YOR351C)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MID2(YLR332W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MMS1(YPR164W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MPC2(YHR162W)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRPL16(YBL038W)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MST27(YGL051W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Gene:ORM1(YGR038W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSW1(YOR255W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PEF1(YGR058W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PMP3(YDR276C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRM6(YML047C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:PUT3(YKL015W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:RKM5(YLR137W)|FD-Score:4.34|P-value:7.06E-6||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:SFB3(YHR098C)|FD-Score:12.6|P-value:6.89E-37||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SMA2(YML066C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SPO1(YNL012W)|FD-Score:-3.73|P-value:9.41E-5||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SWE1(YJL187C)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:YAL004W(YAL004W_d)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBL053W(YBL053W_d)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR284W(YBR284W_p)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YER097W(YER097W_d)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR057W(YFR057W_p)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Putative protein of unknown function Gene:YGL034C(YGL034C_d)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR180W(YHR180W_d)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR005C(YKR005C_p)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Putative protein of unknown function Gene:YLL053C(YLL053C_p)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR173W(YLR173W_p)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Putative protein of unknown function Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Putative protein of unknown function Gene:YNL120C(YNL120C_d)|FD-Score:4.88|P-value:5.40E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNL146W(YNL146W_p)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL197C(YPL197C_d)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:APP1(YNL094W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARG81(YML099C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ATG29(YPL166W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATG3(YNR007C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:BTS1(YPL069C)|FD-Score:-4.86|P-value:5.93E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CRP1(YHR146W)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Protein that binds to cruciform DNA structures Gene:CUS2(YNL286W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DMA2(YNL116W)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DPH1(YIL103W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ERV46(YAL042W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:IRA2(YOL081W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:-4.5|P-value:3.32E-6||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LYS9(YNR050C)|FD-Score:4.27|P-value:9.64E-6||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MDM20(YOL076W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MEK1(YOR351C)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MID2(YLR332W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MMS1(YPR164W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MPC2(YHR162W)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRPL16(YBL038W)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MST27(YGL051W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Gene:ORM1(YGR038W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OSW1(YOR255W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PEF1(YGR058W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PMP3(YDR276C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRM6(YML047C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:PUT3(YKL015W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:RKM5(YLR137W)|FD-Score:4.34|P-value:7.06E-6||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:SFB3(YHR098C)|FD-Score:12.6|P-value:6.89E-37||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SMA2(YML066C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SPO1(YNL012W)|FD-Score:-3.73|P-value:9.41E-5||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SWE1(YJL187C)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:YAL004W(YAL004W_d)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBL053W(YBL053W_d)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR284W(YBR284W_p)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YER097W(YER097W_d)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR057W(YFR057W_p)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Putative protein of unknown function Gene:YGL034C(YGL034C_d)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR180W(YHR180W_d)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR005C(YKR005C_p)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Putative protein of unknown function Gene:YLL053C(YLL053C_p)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR173W(YLR173W_p)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Putative protein of unknown function Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Putative protein of unknown function Gene:YNL120C(YNL120C_d)|FD-Score:4.88|P-value:5.40E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNL146W(YNL146W_p)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL197C(YPL197C_d)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL007C5.715.78E-91.11TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YDR328C4.602.16E-60.08SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YOL038W4.523.10E-60.03PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YLR208W4.493.55E-60.48SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YBR237W4.013.09E-50.17PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YPR112C3.836.32E-50.05MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YMR290W-A_d3.787.83E-50.20YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YGL069C_d3.581.69E-40.50YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YKL196C3.080.001020.08YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YML092C3.010.001320.09PRE8Alpha 2 subunit of the 20S proteasome
YNL026W2.920.001760.10SAM50Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
YDL147W2.810.002460.02RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YDL195W2.790.002600.02SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YIL046W2.780.002730.03MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YDL097C2.750.002950.01RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR098C12.606.89E-37SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YKL126W5.491.98E-8YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YNL120C_d4.885.40E-7YNL120C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene
YOL076W4.671.52E-6MDM20Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly
YNL116W4.572.48E-6DMA2Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains
YJL187C4.532.97E-6SWE1Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate
YLR137W4.347.06E-6RKM5Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species
YNR050C4.279.64E-6LYS9Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
YNL094W4.201.35E-5APP1Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway
YHR146W4.181.49E-5CRP1Protein that binds to cruciform DNA structures
YKL015W4.092.13E-5PUT3Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
YOR351C4.082.23E-5MEK1Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids
YKR005C_p4.032.76E-5YKR005C_pPutative protein of unknown function
YBL038W3.787.78E-5MRPL16Mitochondrial ribosomal protein of the large subunit
YPL166W3.787.95E-5ATG29Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

GO enrichment analysis for SGTC_307
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2106.15E-60SGTC_9831491-0821 12.2 μMChemDiv (Drug-like library)464951130.583333
0.1353.04E-25SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.444444ERG2
0.1331.21E-24SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.351852
0.1286.44E-23SGTC_890302-0250 142.1 μMChemDiv (Drug-like library)42035180.0461538
0.1133.36E-18SGTC_2745rifampicin 24.3 μMMiscellaneous251414280.046875
0.1119.74E-18SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0574713
0.1102.06E-17SGTC_592k241-0073 46.5 μMChemDiv (Drug-like library)42301580.0434783
0.1103.01E-17SGTC_8550428-0020 25.0 μMChemDiv (Drug-like library)68124140.0806452Golgi
0.1095.42E-17SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.0821918
0.1049.08E-16SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.0909091
0.1041.02E-15SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0675676
0.1024.35E-15SGTC_7874152-0075 138.0 μMChemDiv (Drug-like library)95665390.0704225
0.0912.85E-12SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.0625calcium & mitochondrial duress
0.0904.61E-12SGTC_7864544-0069 423.0 μMChemDiv (Drug-like library)7488210.0757576
0.0864.05E-11SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.0985916

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_9831491-082112.2 μM0.58333346495113ChemDiv (Drug-like library)440.5220435.24623
SGTC_6341611-431711.2 μM0.4444442843522ChemDiv (Drug-like library)475.559774.82423ERG2
SGTC_7891309-113570.6 μM0.4363643092424ChemDiv (Drug-like library)379.068643.80623ERG2
SGTC_9681309-0633340 μM0.4333333761976ChemDiv (Drug-like library)391.633722.98324
SGTC_20635246145200 μM0.38711240Chembridge (Fragment library)221.639722.67412
SGTC_9322857-0803154 μM0.365385674898ChemDiv (Drug-like library)280.11732.55112
SGTC_20255147754141 μM0.351852315777Chembridge (Fragment library)229.231341.74913
SGTC_23246149169200 μM0.3275862888231Chembridge (Fragment library)247.273062.0124
SGTC_21255246583200 μM0.327273835267Chembridge (Fragment library)202.20931.88413tubulin folding & SWR complex
SGTC_3211912878149.47 μM0.32203417194361Chembridge (Drug-like library)294.756643.55423