9116018

N-[2-(4-acetylpiperazin-1-yl)-3-chlorophenyl]-3,4-dimethylbenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3074
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17027303
SMILES CC1=C(C=C(C=C1)C(=O)NC2=C(C(=CC=C2)Cl)N3CCN(CC3)C(=O)C)C
Standardized SMILES CC(=O)N1CCN(CC1)c2c(Cl)cccc2NC(=O)c3ccc(C)c(C)c3
Molecular weight 385.8872
ALogP 3.63
H-bond donor count 1
H-bond acceptor count 3
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.99
% growth inhibition (Hom. pool) 8.08


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17027303
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:4.56|P-value:2.57E-6|Clearance:0.12||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:BET5(YML077W)|FD-Score:-3.87|P-value:5.46E-5|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CDC39(YCR093W)|FD-Score:-3.34|P-value:4.26E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DML1(YMR211W)|FD-Score:5.73|P-value:5.15E-9|Clearance:1.17||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GLC7(YER133W)|FD-Score:4.44|P-value:4.42E-6|Clearance:0.04||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GLN4(YOR168W)|FD-Score:4.4|P-value:5.30E-6|Clearance:0.07||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:LSM2(YBL026W)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MDN1(YLR106C)|FD-Score:-3.31|P-value:4.66E-4|Clearance:0||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:PDS5(YMR076C)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.14||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:POL31(YJR006W)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:RNA14(YMR061W)|FD-Score:3.22|P-value:6.39E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC82(YPR190C)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.1||SGD DESC:RNA polymerase III subunit C82 Gene:RPP1(YHR062C)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:-3.23|P-value:6.24E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SEC11(YIR022W)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.02||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SEC17(YBL050W)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SSS1(YDR086C)|FD-Score:-4.32|P-value:7.96E-6|Clearance:0||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TAF9(YMR236W)|FD-Score:4.34|P-value:7.26E-6|Clearance:1.03||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:ACS2(YLR153C)|FD-Score:4.56|P-value:2.57E-6|Clearance:0.12||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:BET5(YML077W)|FD-Score:-3.87|P-value:5.46E-5|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CDC39(YCR093W)|FD-Score:-3.34|P-value:4.26E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DML1(YMR211W)|FD-Score:5.73|P-value:5.15E-9|Clearance:1.17||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GLC7(YER133W)|FD-Score:4.44|P-value:4.42E-6|Clearance:0.04||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GLN4(YOR168W)|FD-Score:4.4|P-value:5.30E-6|Clearance:0.07||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:LSM2(YBL026W)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MDN1(YLR106C)|FD-Score:-3.31|P-value:4.66E-4|Clearance:0||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:PDS5(YMR076C)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.14||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:POL31(YJR006W)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:RNA14(YMR061W)|FD-Score:3.22|P-value:6.39E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC82(YPR190C)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.1||SGD DESC:RNA polymerase III subunit C82 Gene:RPP1(YHR062C)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS3(YNL178W)|FD-Score:-3.23|P-value:6.24E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SEC11(YIR022W)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.02||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SEC17(YBL050W)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SSS1(YDR086C)|FD-Score:-4.32|P-value:7.96E-6|Clearance:0||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TAF9(YMR236W)|FD-Score:4.34|P-value:7.26E-6|Clearance:1.03||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17027303
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ1(YNR033W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:ALD6(YPL061W)|FD-Score:-5.64|P-value:8.60E-9||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATO3(YDR384C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BRE1(YDL074C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BST1(YFL025C)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CHS7(YHR142W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLB2(YPR119W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLG1(YGL215W)|FD-Score:5.23|P-value:8.43E-8||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:DSK2(YMR276W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:FMP27(YLR454W_p)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUR4(YBR021W)|FD-Score:7.55|P-value:2.13E-14||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV12(YOR183W_p)|FD-Score:-3.87|P-value:5.51E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GTO1(YGR154C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HCS1(YKL017C)|FD-Score:-4.35|P-value:6.86E-6||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HIS6(YIL020C)|FD-Score:-3.76|P-value:8.45E-5||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HUA1(YGR268C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IDP1(YDL066W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:JEM1(YJL073W)|FD-Score:4.8|P-value:8.00E-7||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KAP114(YGL241W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KEL1(YHR158C)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KIP2(YPL155C)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:KTR7(YIL085C)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LAA1(YJL207C)|FD-Score:-3.83|P-value:6.30E-5||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LEM3(YNL323W)|FD-Score:5.72|P-value:5.29E-9||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM6(YDR378C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LYS12(YIL094C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MCK1(YNL307C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MCM21(YDR318W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MET10(YFR030W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MRP51(YPL118W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MSH2(YOL090W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NGG1(YDR176W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NIT3(YLR351C)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NOT3(YIL038C)|FD-Score:-4.27|P-value:9.66E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PAF1(YBR279W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PDR1(YGL013C)|FD-Score:4.48|P-value:3.80E-6||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDX3(YBR035C)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PFK1(YGR240C)|FD-Score:6.14|P-value:4.14E-10||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:RIT1(YMR283C)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RSC1(YGR056W)|FD-Score:9.52|P-value:8.91E-22||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SGE1(YPR198W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIL1(YOL031C)|FD-Score:-4.34|P-value:7.03E-6||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKT5(YBL061C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLG1(YOR008C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SRF1(YDL133W)|FD-Score:4.29|P-value:9.11E-6||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSD1(YDR293C)|FD-Score:12|P-value:2.91E-33||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SVL3(YPL032C)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TOS1(YBR162C)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TSC3(YBR058C-A)|FD-Score:5.31|P-value:5.62E-8||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP18(YMR071C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMA22(YHR060W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VMA4(YOR332W)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS21(YOR089C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS71(YML041C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS73(YGL104C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBP2(YGL060W)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YDR149C(YDR149C_d)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YER119C-A(YER119C-A_d)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL260W(YGL260W_p)|FD-Score:-4.87|P-value:5.53E-7||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR291C(YGR291C_d)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHL044W(YHL044W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YLL020C(YLL020C_d)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR090W(YMR090W_p)|FD-Score:4.91|P-value:4.67E-7||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR173W-A(YMR173W-A_d)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR230W-A(YMR230W-A_p)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function Gene:YOR082C(YOR082C_d)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPR015C(YPR015C_p)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR099C(YPR099C_d)|FD-Score:-3.66|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:ABZ1(YNR033W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:ALD6(YPL061W)|FD-Score:-5.64|P-value:8.60E-9||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATO3(YDR384C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BRE1(YDL074C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BST1(YFL025C)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CHS7(YHR142W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLB2(YPR119W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLG1(YGL215W)|FD-Score:5.23|P-value:8.43E-8||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:DSK2(YMR276W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:FMP27(YLR454W_p)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUR4(YBR021W)|FD-Score:7.55|P-value:2.13E-14||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV12(YOR183W_p)|FD-Score:-3.87|P-value:5.51E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GTO1(YGR154C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HCS1(YKL017C)|FD-Score:-4.35|P-value:6.86E-6||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HIS6(YIL020C)|FD-Score:-3.76|P-value:8.45E-5||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HUA1(YGR268C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IDP1(YDL066W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:JEM1(YJL073W)|FD-Score:4.8|P-value:8.00E-7||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KAP114(YGL241W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:KEL1(YHR158C)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KIP2(YPL155C)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:KTR7(YIL085C)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LAA1(YJL207C)|FD-Score:-3.83|P-value:6.30E-5||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LEM3(YNL323W)|FD-Score:5.72|P-value:5.29E-9||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM6(YDR378C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LYS12(YIL094C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MCK1(YNL307C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MCM21(YDR318W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MET10(YFR030W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MRP51(YPL118W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MSH2(YOL090W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NGG1(YDR176W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NIT3(YLR351C)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NOT3(YIL038C)|FD-Score:-4.27|P-value:9.66E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PAF1(YBR279W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PDR1(YGL013C)|FD-Score:4.48|P-value:3.80E-6||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDX3(YBR035C)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PFK1(YGR240C)|FD-Score:6.14|P-value:4.14E-10||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:RIT1(YMR283C)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RSC1(YGR056W)|FD-Score:9.52|P-value:8.91E-22||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SGE1(YPR198W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIL1(YOL031C)|FD-Score:-4.34|P-value:7.03E-6||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKT5(YBL061C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLG1(YOR008C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SRF1(YDL133W)|FD-Score:4.29|P-value:9.11E-6||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSD1(YDR293C)|FD-Score:12|P-value:2.91E-33||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SVL3(YPL032C)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TOS1(YBR162C)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TSC3(YBR058C-A)|FD-Score:5.31|P-value:5.62E-8||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP18(YMR071C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMA22(YHR060W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VMA4(YOR332W)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS21(YOR089C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS71(YML041C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS73(YGL104C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBP2(YGL060W)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YDR149C(YDR149C_d)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YER119C-A(YER119C-A_d)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL260W(YGL260W_p)|FD-Score:-4.87|P-value:5.53E-7||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR291C(YGR291C_d)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHL044W(YHL044W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YLL020C(YLL020C_d)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR090W(YMR090W_p)|FD-Score:4.91|P-value:4.67E-7||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR173W-A(YMR173W-A_d)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR230W-A(YMR230W-A_p)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function Gene:YOR082C(YOR082C_d)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPR015C(YPR015C_p)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR099C(YPR099C_d)|FD-Score:-3.66|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR211W5.735.15E-91.17DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YLR153C4.562.57E-60.12ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YER133W4.444.42E-60.04GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YOR168W4.405.30E-60.07GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YMR236W4.347.26E-61.03TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YHR062C3.314.69E-40.09RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR061W3.226.39E-41.64E-4RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YIR022W3.226.39E-40.02SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YPR190C3.216.74E-40.10RPC82RNA polymerase III subunit C82
YMR076C3.119.47E-40.14PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YDR473C2.960.001530.11PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YPL083C2.850.002160.02SEN54Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YBR142W2.840.002280.02MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YDR188W2.820.002420.01CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YOR206W2.800.002530.02NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR293C12.002.91E-33SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YGR056W9.528.91E-22RSC1Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
YBR021W7.552.13E-14FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YGR240C6.144.14E-10PFK1Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
YNL323W5.725.29E-9LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
YBR058C-A5.315.62E-8TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGL215W5.238.43E-8CLG1Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2
YMR090W_p4.914.67E-7YMR090W_pPutative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
YJL073W4.808.00E-7JEM1DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
YPL118W4.691.39E-6MRP51Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
YDR378C4.503.37E-6LSM6Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YGL013C4.483.80E-6PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YER119C-A_d4.434.75E-6YER119C-A_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
YDL133W4.299.11E-6SRF1Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
YMR173W-A_d4.241.11E-5YMR173W-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W

GO enrichment analysis for SGTC_3074
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2243.19E-68SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.168831RNA processing & uracil transport
0.1956.84E-52SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.25PDR1
0.1762.30E-42SGTC_33119131146 2.9 μMChembridge (Drug-like library)49004060.0804598cell wall
0.1751.09E-41SGTC_33509150499 3.6 μMChembridge (Drug-like library)49109960.076087cell wall
0.1661.58E-37SGTC_33219137793 26.8 μMChembridge (Drug-like library)49041920.0909091cell wall
0.1611.88E-35SGTC_23679070478 135.4 μMChembridge (Fragment library)5728020.112676cell wall
0.1552.69E-33SGTC_33229139058 2.1 μMChembridge (Drug-like library)49089260.0786517cell wall
0.1532.76E-32SGTC_6660272-0154 136.0 μMChemDiv (Drug-like library)68703120.113924cell wall
0.1533.89E-32SGTC_9282764-0428 58.7 μMChemDiv (Drug-like library)6625780.0365854cell wall
0.1498.47E-31SGTC_33569151811 4.5 μMChembridge (Drug-like library)18592540.0888889cell wall
0.1411.43E-27SGTC_8630466-0191 129.0 μMChemDiv (Drug-like library)7651600.109589cell wall
0.1403.73E-27SGTC_22206782180 200.0 μMChembridge (Fragment library)8338030.0810811RNA processing & uracil transport
0.1395.13E-27SGTC_33519150312 21.6 μMChembridge (Drug-like library)49049050.0823529cell wall
0.1397.07E-27SGTC_33559151058 44.0 μMChembridge (Drug-like library)170157280.171053RNA processing & uracil transport
0.1343.82E-25SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.0833333RNA processing & uracil transport

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3066911724549.47 μM0.7817176980Chembridge (Drug-like library)371.860583.14413
SGTC_3114912276649.47 μM0.62517176985Chembridge (Drug-like library)375.8244632.86314
SGTC_2861902459519.48 μM0.51562516482810Chembridge (Drug-like library)401.886562.9914
SGTC_2809796413312.99 μM0.4615381253665Chembridge (Drug-like library)420.332224.4751360S ribosome export
SGTC_2822799471871.43 μM0.4090911018011Chembridge (Drug-like library)373.876463.58214
SGTC_22016605497169.86 μM0.407407670741Chembridge (Fragment library)232.32142.18102ergosterol biosynthesis
SGTC_3285911281820.87 μM0.37681217017947Chembridge (Drug-like library)408.899043.29615cell wall
SGTC_3236913270149.47 μM0.36231924282524Chembridge (Drug-like library)378.832.57614TSC3-RPN4
SGTC_3186911046449.47 μM0.35593217018021Chembridge (Drug-like library)269.338264.05612RPP1 & pyrimidine depletion
SGTC_3176910658249.47 μM0.35483917018027Chembridge (Drug-like library)275.730163.74812