9117319

4-methoxy-N-(3-propan-2-yloxyphenyl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3077
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17154678
SMILES CC(C)OC1=CC=CC(=C1)NC(=O)C2=CC=C(C=C2)OC
Standardized SMILES COc1ccc(cc1)C(=O)Nc2cccc(OC(C)C)c2
Molecular weight 285.3377
ALogP 3.31
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.71
% growth inhibition (Hom. pool) 5.9


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17154678
Download HIP data (tab-delimited text)  (excel)
Gene:GCD1(YOR260W)|FD-Score:3.94|P-value:4.10E-5|Clearance:0.21||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD7(YLR291C)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.36||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:ILV5(YLR355C)|FD-Score:5.28|P-value:6.50E-8|Clearance:0.56||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MCM2(YBL023C)|FD-Score:3.73|P-value:9.67E-5|Clearance:0.2||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MTR2(YKL186C)|FD-Score:5.71|P-value:5.56E-9|Clearance:0.56||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:POP6(YGR030C)|FD-Score:-4.31|P-value:8.09E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP11(YDL043C)|FD-Score:3.96|P-value:3.78E-5|Clearance:0.02||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:PRP6(YBR055C)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.13||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:SSS1(YDR086C)|FD-Score:-3.85|P-value:5.89E-5|Clearance:0||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:SWD2(YKL018W)|FD-Score:-3.31|P-value:4.60E-4|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TAF10(YDR167W)|FD-Score:-4.53|P-value:2.99E-6|Clearance:0||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TIF35(YDR429C)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.03||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRS20(YBR254C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.42||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:TSC10(YBR265W)|FD-Score:5.37|P-value:4.03E-8|Clearance:0.56||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:TSR1(YDL060W)|FD-Score:4.34|P-value:7.14E-6|Clearance:0.38||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YJU2(YKL095W)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:GCD1(YOR260W)|FD-Score:3.94|P-value:4.10E-5|Clearance:0.21||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD7(YLR291C)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.36||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:ILV5(YLR355C)|FD-Score:5.28|P-value:6.50E-8|Clearance:0.56||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MCM2(YBL023C)|FD-Score:3.73|P-value:9.67E-5|Clearance:0.2||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MTR2(YKL186C)|FD-Score:5.71|P-value:5.56E-9|Clearance:0.56||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:POP6(YGR030C)|FD-Score:-4.31|P-value:8.09E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP11(YDL043C)|FD-Score:3.96|P-value:3.78E-5|Clearance:0.02||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:PRP6(YBR055C)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.13||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:SSS1(YDR086C)|FD-Score:-3.85|P-value:5.89E-5|Clearance:0||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:SWD2(YKL018W)|FD-Score:-3.31|P-value:4.60E-4|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TAF10(YDR167W)|FD-Score:-4.53|P-value:2.99E-6|Clearance:0||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TIF35(YDR429C)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.03||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRS20(YBR254C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.42||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:TSC10(YBR265W)|FD-Score:5.37|P-value:4.03E-8|Clearance:0.56||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:TSR1(YDL060W)|FD-Score:4.34|P-value:7.14E-6|Clearance:0.38||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YJU2(YKL095W)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17154678
Download HOP data (tab-delimited text)  (excel)
Gene:ARO2(YGL148W)|FD-Score:4.31|P-value:8.04E-6||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BOI1(YBL085W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BTN2(YGR142W)|FD-Score:-5.22|P-value:9.06E-8||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CDC26(YFR036W)|FD-Score:4.3|P-value:8.35E-6||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COA2(YPL189C-A)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG8(YML071C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX19(YLL018C-A)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DCI1(YOR180C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DCV1(YFR012W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DFG16(YOR030W)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:ETR1(YBR026C)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FAP1(YNL023C)|FD-Score:-5|P-value:2.83E-7||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FRE4(YNR060W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GAL7(YBR018C)|FD-Score:4.38|P-value:5.96E-6||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GPT2(YKR067W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GSC2(YGR032W)|FD-Score:-4.87|P-value:5.72E-7||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GYP1(YOR070C)|FD-Score:6.6|P-value:2.04E-11||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HLJ1(YMR161W)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:KTR7(YIL085C)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MGR2(YPL098C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MOT3(YMR070W)|FD-Score:-7.4|P-value:6.88E-14||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRPL16(YBL038W)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA2(YKR077W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSS51(YLR203C)|FD-Score:-5.89|P-value:1.98E-9||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MUP1(YGR055W)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NAT3(YPR131C)|FD-Score:-3.79|P-value:7.63E-5||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NTG1(YAL015C)|FD-Score:-4.36|P-value:6.36E-6||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:OPI8(YKR035C_d)|FD-Score:6.52|P-value:3.57E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OYE2(YHR179W)|FD-Score:5.36|P-value:4.22E-8||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PES4(YFR023W)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PRO1(YDR300C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PTP1(YDL230W)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:REG1(YDR028C)|FD-Score:7.24|P-value:2.17E-13||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFM1(YOR279C)|FD-Score:5.27|P-value:6.88E-8||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGP1(YDR137W)|FD-Score:4.92|P-value:4.39E-7||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RML2(YEL050C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPS26B(YER131W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RSR1(YGR152C)|FD-Score:-5.39|P-value:3.49E-8||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:RTN1(YDR233C)|FD-Score:-5.05|P-value:2.18E-7||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SER1(YOR184W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SGO1(YOR073W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SIL1(YOL031C)|FD-Score:-4.84|P-value:6.42E-7||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SNF7(YLR025W)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SRL1(YOR247W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP4(YDR354W)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:VPS61(YDR136C_d)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:WHI4(YDL224C)|FD-Score:-3.84|P-value:6.27E-5||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YBR116C(YBR116C_d)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR300C(YBR300C_d)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YCR095W-A(YCR095W-A_p)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Putative protein of unknown function Gene:YDR008C(YDR008C_d)|FD-Score:-5.66|P-value:7.37E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR124W(YDR124W_p)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR133C(YDR133C_d)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR514C(YDR514C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YGL260W(YGL260W_p)|FD-Score:-4.74|P-value:1.04E-6||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR117C(YGR117C_p)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIL059C(YIL059C_d)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Gene:YJL070C(YJL070C_p)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YJL169W(YJL169W_d)|FD-Score:-4.57|P-value:2.45E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-3.73|P-value:9.58E-5||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR056C(YJR056C_p)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YKR045C(YKR045C_p)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YMR230W-A(YMR230W-A_p)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function Gene:YMR304C-A(YMR304C-A_d)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YOR050C(YOR050C_d)|FD-Score:-3.86|P-value:5.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YOR283W(YOR283W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YOR302W(YOR302W)|FD-Score:-4.47|P-value:3.85E-6||SGD DESC:CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA Gene:YPL014W(YPL014W_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:ZPS1(YOL154W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH Gene:ARO2(YGL148W)|FD-Score:4.31|P-value:8.04E-6||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BOI1(YBL085W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BTN2(YGR142W)|FD-Score:-5.22|P-value:9.06E-8||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CDC26(YFR036W)|FD-Score:4.3|P-value:8.35E-6||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COA2(YPL189C-A)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG8(YML071C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX19(YLL018C-A)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DCI1(YOR180C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DCV1(YFR012W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DFG16(YOR030W)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:ETR1(YBR026C)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FAP1(YNL023C)|FD-Score:-5|P-value:2.83E-7||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FRE4(YNR060W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GAL7(YBR018C)|FD-Score:4.38|P-value:5.96E-6||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GPT2(YKR067W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GSC2(YGR032W)|FD-Score:-4.87|P-value:5.72E-7||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GYP1(YOR070C)|FD-Score:6.6|P-value:2.04E-11||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HLJ1(YMR161W)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:KTR7(YIL085C)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MGR2(YPL098C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MOT3(YMR070W)|FD-Score:-7.4|P-value:6.88E-14||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRPL16(YBL038W)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA2(YKR077W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSS51(YLR203C)|FD-Score:-5.89|P-value:1.98E-9||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MUP1(YGR055W)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NAT3(YPR131C)|FD-Score:-3.79|P-value:7.63E-5||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NTG1(YAL015C)|FD-Score:-4.36|P-value:6.36E-6||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:OPI8(YKR035C_d)|FD-Score:6.52|P-value:3.57E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OYE2(YHR179W)|FD-Score:5.36|P-value:4.22E-8||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PES4(YFR023W)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PRO1(YDR300C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PTP1(YDL230W)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:REG1(YDR028C)|FD-Score:7.24|P-value:2.17E-13||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFM1(YOR279C)|FD-Score:5.27|P-value:6.88E-8||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGP1(YDR137W)|FD-Score:4.92|P-value:4.39E-7||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RML2(YEL050C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPS26B(YER131W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RRP8(YDR083W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RSR1(YGR152C)|FD-Score:-5.39|P-value:3.49E-8||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:RTN1(YDR233C)|FD-Score:-5.05|P-value:2.18E-7||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SER1(YOR184W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SGO1(YOR073W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SIL1(YOL031C)|FD-Score:-4.84|P-value:6.42E-7||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SNF7(YLR025W)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SRL1(YOR247W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP4(YDR354W)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:VPS61(YDR136C_d)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:WHI4(YDL224C)|FD-Score:-3.84|P-value:6.27E-5||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YBR116C(YBR116C_d)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR300C(YBR300C_d)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YCR095W-A(YCR095W-A_p)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Putative protein of unknown function Gene:YDR008C(YDR008C_d)|FD-Score:-5.66|P-value:7.37E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR124W(YDR124W_p)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR133C(YDR133C_d)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR514C(YDR514C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YGL260W(YGL260W_p)|FD-Score:-4.74|P-value:1.04E-6||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR117C(YGR117C_p)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIL059C(YIL059C_d)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Gene:YJL070C(YJL070C_p)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YJL169W(YJL169W_d)|FD-Score:-4.57|P-value:2.45E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-3.73|P-value:9.58E-5||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR056C(YJR056C_p)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YKR045C(YKR045C_p)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YMR230W-A(YMR230W-A_p)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function Gene:YMR304C-A(YMR304C-A_d)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YOR050C(YOR050C_d)|FD-Score:-3.86|P-value:5.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YOR283W(YOR283W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YOR302W(YOR302W)|FD-Score:-4.47|P-value:3.85E-6||SGD DESC:CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA Gene:YPL014W(YPL014W_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:ZPS1(YOL154W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YKL186C5.715.56E-90.56MTR2mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
YBR265W5.374.03E-80.56TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YLR355C5.286.50E-80.56ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YDR429C4.721.16E-60.03TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YLR291C4.701.32E-60.36GCD7Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YDL060W4.347.14E-60.38TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDL043C3.963.78E-50.02PRP11Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YOR260W3.944.10E-50.21GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YBL023C3.739.67E-50.20MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YBR254C3.532.11E-40.42TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
YBR055C3.119.43E-40.13PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YCL054W2.970.001470.05SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YLR316C2.920.001740.03TAD3Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs
YOR272W2.900.001880.04YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YBL041W2.860.002120.16PRE7Beta 6 subunit of the 20S proteasome

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR028C7.242.17E-13REG1Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
YOR070C6.602.04E-11GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YKR035C_d6.523.57E-11OPI8_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
YHR179W5.364.22E-8OYE2Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress
YOR279C5.276.88E-8RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YLR025W5.091.80E-7SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YDR137W4.924.39E-7RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YOR030W4.513.31E-6DFG16Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
YBL038W4.434.69E-6MRPL16Mitochondrial ribosomal protein of the large subunit
YMR161W4.424.91E-6HLJ1Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
YBR018C4.385.96E-6GAL7Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism
YBR026C4.376.19E-6ETR12-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis
YGL148W4.318.04E-6ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YFR036W4.308.35E-6CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YDR159W4.221.24E-5SAC3Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)

GO enrichment analysis for SGTC_3077
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1478.39E-30SGTC_30709117005 49.5 μMChembridge (Drug-like library)87790160.574468
0.0814.04E-10SGTC_33209137005 35.1 μMChembridge (Drug-like library)49087130.0657895endomembrane recycling
0.0806.07E-10SGTC_30789116413 49.5 μMChembridge (Drug-like library)171773220.197368unfolded protein response
0.0773.11E-9SGTC_18845633444 25.0 μMMiscellaneous57242390.132353redox potentiating
0.0741.15E-8SGTC_29005328528 85.3 μMChembridge (Drug-like library)7953590.565217Golgi
0.0706.20E-8SGTC_1240136-0217 7.1 μMChemDiv (Drug-like library)39769050.183099mitochondrial stress
0.0691.18E-7SGTC_1190121-0043 24.8 μMChemDiv (Drug-like library)10923780.275862mitochondrial stress
0.0681.50E-7SGTC_7814335-2806 94.4 μMChemDiv (Drug-like library)8452540.084507
0.0681.62E-7SGTC_392N-ethylmaleimide 8.3 μMMiscellaneous43620.0816327RNA pol III & RNase P/MRP
0.0663.32E-7SGTC_1664st014217 9.0 μMTimTec (Natural product derivative library)39827820.073170760S ribosome export
0.0664.28E-7SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.19697Golgi
0.0655.17E-7SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0833333
0.0656.87E-7SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.425926
0.0648.67E-7SGTC_5571315-0110 16.2 μMChemDiv (Drug-like library)68338010.169231Golgi
0.0631.30E-6SGTC_24365633522 8.1 μMMiscellaneous57242420.0735294

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2999906976571.43 μM0.8974362097066Chembridge (Drug-like library)285.337663.30813amide catabolism
SGTC_2986902244571.43 μM0.8048786459286Chembridge (Drug-like library)285.337663.30813
SGTC_2877904072471.43 μM0.7619056463895Chembridge (Drug-like library)285.337663.30813
SGTC_2871903851012.99 μM0.5918374945164Chembridge (Drug-like library)283.321783.19813
SGTC_2881905895351.95 μM0.59183717122249Chembridge (Drug-like library)281.30593.77413
SGTC_3098911903949.47 μM0.59183717161528Chembridge (Drug-like library)287.3287234.05313Golgi
SGTC_3067911743149.47 μM0.58695717150885Chembridge (Drug-like library)285.337663.30813
SGTC_3070911700549.47 μM0.5744688779016Chembridge (Drug-like library)273.3021433.5313
SGTC_3205911348349.47 μM0.57446817018033Chembridge (Drug-like library)271.311083.06813
SGTC_2851901592271.43 μM0.5714292996893Chembridge (Drug-like library)299.364243.31513