9117008

3-(4-methylphenyl)-N-pyridin-3-ylpropanamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3082
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 41448529
SMILES CC1=CC=C(C=C1)CCC(=O)NC2=CN=CC=C2
Standardized SMILES Cc1ccc(CCC(=O)Nc2cccnc2)cc1
Molecular weight 240.3003
ALogP 2.44
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.47
% growth inhibition (Hom. pool) 8.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 41448529
Download HIP data (tab-delimited text)  (excel)
Gene:GLC7(YER133W)|FD-Score:-3.14|P-value:8.52E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LAS1(YKR063C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.07||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:NMD3(YHR170W)|FD-Score:-3.85|P-value:5.95E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC4(YPR144C)|FD-Score:5.06|P-value:2.14E-7|Clearance:0.92||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:NSE4(YDL105W)|FD-Score:4.14|P-value:1.77E-5|Clearance:0.15||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PFY1(YOR122C)|FD-Score:-3.8|P-value:7.25E-5|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:-3.58|P-value:1.71E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC4(YFL005W)|FD-Score:-3.1|P-value:9.82E-4|Clearance:0||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:STT4(YLR305C)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:TRM5(YHR070W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:YKL153W(YKL153W_d)|FD-Score:3.99|P-value:3.34E-5|Clearance:0.87||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:GLC7(YER133W)|FD-Score:-3.14|P-value:8.52E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LAS1(YKR063C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.07||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:NMD3(YHR170W)|FD-Score:-3.85|P-value:5.95E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC4(YPR144C)|FD-Score:5.06|P-value:2.14E-7|Clearance:0.92||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:NSE4(YDL105W)|FD-Score:4.14|P-value:1.77E-5|Clearance:0.15||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PFY1(YOR122C)|FD-Score:-3.8|P-value:7.25E-5|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:-3.58|P-value:1.71E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC4(YFL005W)|FD-Score:-3.1|P-value:9.82E-4|Clearance:0||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:STT4(YLR305C)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:TRM5(YHR070W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:YKL153W(YKL153W_d)|FD-Score:3.99|P-value:3.34E-5|Clearance:0.87||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 41448529
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:4.37|P-value:6.35E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AKL1(YBR059C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:APJ1(YNL077W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ATP7(YKL016C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BGL2(YGR282C)|FD-Score:-4.36|P-value:6.58E-6||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BUD9(YGR041W)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole Gene:CCR4(YAL021C)|FD-Score:5.23|P-value:8.26E-8||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG7(YGL005C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ERG5(YMR015C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:GCN3(YKR026C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GMC1(YDR506C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:IMA1(YGR287C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IME2(YJL106W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p Gene:IRC25(YLR021W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRS4(YKR019C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LDB16(YCL005W)|FD-Score:6.46|P-value:5.23E-11||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LEU4(YNL104C)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MMM1(YLL006W)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MSC7(YHR039C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSN2(YMR037C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MUB1(YMR100W)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NRM1(YNR009W)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:OAZ1(YPL052W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PCK1(YKR097W)|FD-Score:4.28|P-value:9.25E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PEF1(YGR058W)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PEP8(YJL053W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PGD1(YGL025C)|FD-Score:-4.3|P-value:8.43E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PRO2(YOR323C)|FD-Score:6.2|P-value:2.90E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RGI1(YER067W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RIM21(YNL294C)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL8A(YHL033C)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.33|P-value:7.30E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SCW4(YGR279C)|FD-Score:6.15|P-value:3.92E-10||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SKG1(YKR100C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SLH1(YGR271W)|FD-Score:6.31|P-value:1.43E-10||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SNF7(YLR025W)|FD-Score:5.47|P-value:2.24E-8||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SWE1(YJL187C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:TAT2(YOL020W)|FD-Score:4.27|P-value:9.96E-6||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:UBA3(YPR066W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:URM1(YIL008W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS61(YDR136C_d)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:XYL2(YLR070C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YAK1(YJL141C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YAP6(YDR259C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication Gene:YBR090C(YBR090C_p)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YBR242W(YBR242W_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL023C(YDL023C_d)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL071C(YDL071C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDL162C(YDL162C_d)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YGR228W(YGR228W_d)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHL037C(YHL037C_d)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL181W(YJL181W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YJR154W(YJR154W_p)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR428C(YLR428C_d)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YPK1(YKL126W)|FD-Score:4.89|P-value:5.16E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL257W(YPL257W_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:AFT1(YGL071W)|FD-Score:4.37|P-value:6.35E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AKL1(YBR059C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:APJ1(YNL077W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ATP7(YKL016C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BGL2(YGR282C)|FD-Score:-4.36|P-value:6.58E-6||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BUD9(YGR041W)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole Gene:CCR4(YAL021C)|FD-Score:5.23|P-value:8.26E-8||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG7(YGL005C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ERG5(YMR015C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:GCN3(YKR026C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GMC1(YDR506C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Gene:IMA1(YGR287C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IME2(YJL106W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p Gene:IRC25(YLR021W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRS4(YKR019C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LDB16(YCL005W)|FD-Score:6.46|P-value:5.23E-11||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LEU4(YNL104C)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MMM1(YLL006W)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MSC7(YHR039C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSN2(YMR037C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MUB1(YMR100W)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NRM1(YNR009W)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:OAZ1(YPL052W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PCK1(YKR097W)|FD-Score:4.28|P-value:9.25E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PEF1(YGR058W)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PEP8(YJL053W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PGD1(YGL025C)|FD-Score:-4.3|P-value:8.43E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PRO2(YOR323C)|FD-Score:6.2|P-value:2.90E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RGI1(YER067W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RIM21(YNL294C)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL8A(YHL033C)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.33|P-value:7.30E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SCW4(YGR279C)|FD-Score:6.15|P-value:3.92E-10||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SKG1(YKR100C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SLH1(YGR271W)|FD-Score:6.31|P-value:1.43E-10||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SNF7(YLR025W)|FD-Score:5.47|P-value:2.24E-8||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SWE1(YJL187C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:TAT2(YOL020W)|FD-Score:4.27|P-value:9.96E-6||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:UBA3(YPR066W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:URM1(YIL008W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS61(YDR136C_d)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:XYL2(YLR070C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YAK1(YJL141C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YAP6(YDR259C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication Gene:YBR090C(YBR090C_p)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YBR242W(YBR242W_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL023C(YDL023C_d)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL071C(YDL071C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDL162C(YDL162C_d)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YGR228W(YGR228W_d)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHL037C(YHL037C_d)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL181W(YJL181W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YJR154W(YJR154W_p)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR428C(YLR428C_d)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YPK1(YKL126W)|FD-Score:4.89|P-value:5.16E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL257W(YPL257W_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR144C5.062.14E-70.92NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YDL105W4.141.77E-50.15NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YKL153W_d3.993.34E-50.86YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YKR063C3.128.97E-40.07LAS1Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
YKR002W3.050.001140.00PAP1Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
YPR181C3.050.001150.16SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YLR355C2.890.001930.06ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YPR133C2.830.002340.01SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YKR071C2.820.002430.00DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YLR100W2.810.002450.03ERG273-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
YMR288W2.790.002660.04HSH155U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
YER125W2.750.003000.04RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
YGL238W2.710.003360.00CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YBR089W_d2.710.003380.05YBR089W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
YNL150W_d2.660.003940.06YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCL005W6.465.23E-11LDB16Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
YGR271W6.311.43E-10SLH1Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses
YOR323C6.202.90E-10PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YGR279C6.153.92E-10SCW4Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating
YLR025W5.472.24E-8SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YAL021C5.238.26E-8CCR4Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
YNL294C4.904.75E-7RIM21pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
YKL126W4.895.16E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR428C_d4.473.88E-6YLR428C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1
YGL071W4.376.35E-6AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YKR097W4.289.25E-6PCK1Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
YOL020W4.279.96E-6TAT2High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance
YPR066W4.211.30E-5UBA3Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YJR154W_p4.062.47E-5YJR154W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YDR259C3.944.14E-5YAP6Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3082
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1217.54E-21SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.0869565
0.1171.68E-19SGTC_18845633444 25.0 μMMiscellaneous57242390.245902redox potentiating
0.1172.32E-19SGTC_31059121040 49.5 μMChembridge (Drug-like library)414489070.744186
0.1155.95E-19SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0987654
0.1081.09E-16SGTC_23749071832 200.0 μMChembridge (Fragment library)173816980.125endomembrane recycling
0.1022.92E-15SGTC_10441222-0021 1.8 μMChemDiv (Drug-like library)43067030.153846heme biosynthesis & mitochondrial translocase
0.0976.91E-14SGTC_1573oleylamine 1.1 μMTimTec (Pure natural product library)53567890.0377358
0.0938.66E-13SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.233333iron homeostasis
0.0931.02E-12SGTC_31759106517 49.5 μMChembridge (Drug-like library)414680730.744186iron homeostasis
0.0913.16E-12SGTC_2501sodium tetradecyl sulfate 13.4 μMMicrosource (Natural product library)236657720.0454545
0.0905.39E-12SGTC_7710250-0039 27.3 μMChemDiv (Drug-like library)30051150.16129
0.0881.11E-11SGTC_29259010536 32.5 μMChembridge (Drug-like library)29941770.163934azole & statin
0.0881.20E-11SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.114286ERG2
0.0848.35E-11SGTC_1847st056231 15.0 μMTimTec (Natural product derivative library)53931800.0909091
0.0831.35E-10SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.108108calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3105912104049.47 μM0.74418641448907Chembridge (Drug-like library)268.35353.14912
SGTC_3175910651749.47 μM0.74418641468073Chembridge (Drug-like library)282.380083.35512iron homeostasis
SGTC_2984902210938.96 μM0.5744686459207Chembridge (Drug-like library)269.338263.57512
SGTC_2969909140828.7 μM0.5416674286311Chembridge (Drug-like library)254.326923.31812
SGTC_20975376186200 μM0.444444742755Chembridge (Fragment library)198.263662.55712
SGTC_22757947463200 μM0.441225414Chembridge (Fragment library)216.235880.85813iron homeostasis
SGTC_3207911416349.47 μM0.423077849233Chembridge (Drug-like library)269.338263.43812
SGTC_20485246248200 μM0.416667677046Chembridge (Fragment library)216.2110631.66913
SGTC_2839900395125.97 μM0.4150942990634Chembridge (Drug-like library)283.364843.81512
SGTC_2968909066333.37 μM0.41071425236811Chembridge (Drug-like library)325.224123.43413