9116877

2-[2-(2,5-dichlorothiophen-3-yl)-2-oxoethyl]sulfanyl-3-methylquinazolin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3084
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 27241325
SMILES CN1C(=O)C2=CC=CC=C2N=C1SCC(=O)C3=C(SC(=C3)Cl)Cl
Standardized SMILES CN1C(=Nc2ccccc2C1=O)SCC(=O)c3cc(Cl)sc3Cl
Molecular weight 385.2881
ALogP 4.07
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.21
% growth inhibition (Hom. pool) 4.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 27241325
Download HIP data (tab-delimited text)  (excel)
Gene:MTR3(YGR158C)|FD-Score:5.7|P-value:6.15E-9|Clearance:2.11||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOC4(YPR144C)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.52||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PFY1(YOR122C)|FD-Score:-3.86|P-value:5.71E-5|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:-5.15|P-value:1.32E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:MTR3(YGR158C)|FD-Score:5.7|P-value:6.15E-9|Clearance:2.11||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOC4(YPR144C)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.52||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PFY1(YOR122C)|FD-Score:-3.86|P-value:5.71E-5|Clearance:0||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:-5.15|P-value:1.32E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 27241325
Download HOP data (tab-delimited text)  (excel)
Gene:ARG7(YMR062C)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:BEM4(YPL161C)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNA1(YJR025C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BUD16(YEL029C)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CBF1(YJR060W)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CNB1(YKL190W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:DEG1(YFL001W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DNF1(YER166W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EFM2(YBR271W)|FD-Score:-4.41|P-value:5.22E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ESC2(YDR363W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:GCS1(YDL226C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GPD2(YOL059W)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HHF1(YBR009C)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HLR1(YDR528W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A; <br>HLR1 has a paralog, LRE1, that arose from the whole genome duplication Gene:HMT1(YBR034C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HXT10(YFL011W)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IBA57(YJR122W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:INP1(YMR204C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IOC3(YFR013W)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:-3.88|P-value:5.21E-5||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LAS21(YJL062W)|FD-Score:8.7|P-value:1.64E-18||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LEU4(YNL104C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MRPL16(YBL038W)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NQM1(YGR043C)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:NSR1(YGR159C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:NUP170(YBL079W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OCA5(YHL029C_p)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI1(YHL020C)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPI3(YJR073C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:RHR2(YIL053W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIM9(YMR063W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RRT2(YBR246W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SAC1(YKL212W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SPF1(YEL031W)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO77(YLR341W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SUR1(YPL057C)|FD-Score:4.5|P-value:3.48E-6||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:TDA5(YLR426W_p)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TEX1(YNL253W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TOS6(YNL300W_p)|FD-Score:-3.9|P-value:4.82E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPS3(YMR261C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS70(YJR126C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YAL066W(YAL066W_d)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBP1(YBR216C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication Gene:YBR056W(YBR056W_p)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YGR015C(YGR015C_p)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YNL195C(YNL195C_p)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR063W(YNR063W_p)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YPL257W(YPL257W_p)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:ARG7(YMR062C)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:BEM4(YPL161C)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNA1(YJR025C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BUD16(YEL029C)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CBF1(YJR060W)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CNB1(YKL190W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:DEG1(YFL001W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DNF1(YER166W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EFM2(YBR271W)|FD-Score:-4.41|P-value:5.22E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ESC2(YDR363W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:GCS1(YDL226C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GPD2(YOL059W)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HHF1(YBR009C)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HLR1(YDR528W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A; <br>HLR1 has a paralog, LRE1, that arose from the whole genome duplication Gene:HMT1(YBR034C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HXT10(YFL011W)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IBA57(YJR122W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:INP1(YMR204C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IOC3(YFR013W)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:-3.88|P-value:5.21E-5||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LAS21(YJL062W)|FD-Score:8.7|P-value:1.64E-18||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LEU4(YNL104C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MRPL16(YBL038W)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NQM1(YGR043C)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:NSR1(YGR159C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:NUP170(YBL079W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OCA5(YHL029C_p)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI1(YHL020C)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPI3(YJR073C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:RHR2(YIL053W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIM9(YMR063W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RRT2(YBR246W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SAC1(YKL212W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SPF1(YEL031W)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO77(YLR341W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SUR1(YPL057C)|FD-Score:4.5|P-value:3.48E-6||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:TDA5(YLR426W_p)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TEX1(YNL253W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TOS6(YNL300W_p)|FD-Score:-3.9|P-value:4.82E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPS3(YMR261C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VPS70(YJR126C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YAL066W(YAL066W_d)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBP1(YBR216C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication Gene:YBR056W(YBR056W_p)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YGR015C(YGR015C_p)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YNL195C(YNL195C_p)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR063W(YNR063W_p)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YPL257W(YPL257W_p)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR158C5.706.15E-92.11MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YPR144C3.591.68E-40.52NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YNL263C3.060.001100.10YIF1Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
YDR365C2.960.001520.25ESF1Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
YKL095W2.720.003300.02YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
YBL076C2.690.003550.08ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YDR301W2.610.004470.04CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YJL005W2.580.004960.07CYR1Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
YNL261W2.510.006010.11ORC5Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YKL089W2.400.008190.01MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
YDR331W2.390.008400.04GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YHR058C2.350.009480.02MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
YDR167W2.330.009910.01TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YKL082C2.320.010300.00RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YNL306W2.310.010400.01MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL062W8.701.64E-18LAS21Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity
YNL229C6.351.09E-10URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YDR528W4.572.43E-6HLR1Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A;
HLR1 has a paralog, LRE1, that arose from the whole genome duplication
YPL057C4.503.48E-6SUR1Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
YNL253W4.405.41E-6TEX1Protein involved in mRNA export, component of the transcription export (TREX) complex
YNR063W_p4.337.34E-6YNR063W_pPutative zinc-cluster protein of unknown function
YBL079W3.885.29E-5NUP170Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
YEL031W3.807.21E-5SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
YJR025C3.701.07E-4BNA13-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YGR043C3.651.34E-4NQM1Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift
YJR126C3.631.44E-4VPS70Protein of unknown function involved in vacuolar protein sorting
YJR073C3.561.88E-4OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YBR034C3.522.12E-4HMT1Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region.
YJR122W3.433.03E-4IBA57Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
YER166W3.363.84E-4DNF1Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase

GO enrichment analysis for SGTC_3084
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1292.96E-23SGTC_683panaxydol 45.0 μMMiscellaneous52832800.0238095sphingolipid biosynthesis & PDR1
0.1111.27E-17SGTC_9551216-0110 33.0 μMChemDiv (Drug-like library)36197190.102564sphingolipid biosynthesis & PDR1
0.1032.09E-15SGTC_30909117696 49.5 μMChembridge (Drug-like library)79238210.166667
0.0961.68E-13SGTC_266formosanin C 406.0 nMMiscellaneous735970.0310078sphingolipid biosynthesis & PDR1
0.0881.60E-11SGTC_28589025781 9.0 μMChembridge (Drug-like library)48949800.0897436sphingolipid biosynthesis & PDR1
0.0813.81E-10SGTC_1941st076221 61.8 μMTimTec (Natural product derivative library)28285680.0625sphingolipid biosynthesis & PDR1
0.0813.89E-10SGTC_14984478-3555 97.6 μMChemDiv (Drug-like library)34379190.0987654
0.0732.35E-8SGTC_1997st073024 93.8 μMTimTec (Natural product derivative library)6862680.112676
0.0715.17E-8SGTC_550767-0285 6.6 μMChemDiv (Drug-like library)31161660.1
0.0715.56E-8SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.09375exosome
0.0715.88E-8SGTC_13461488-2224 62.4 μMChemDiv (Drug-like library)37224270.103896
0.0705.91E-8SGTC_2736butenafine 14.7 μMMiscellaneous24840.0933333
0.0699.29E-8SGTC_590330-0160 1.3 μMChemDiv (Drug-like library)42934820.148148
0.0691.27E-7SGTC_315k915-0449 187.0 μMChemDiv (Drug-like library)209702150.0957447
0.0681.80E-7SGTC_2761levamisole 97.9 μMMiscellaneous268790.0985916

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21685727979200 μM0.480769695210Chembridge (Fragment library)206.26422.23603
SGTC_2832799276251.95 μM0.343752985177Chembridge (Drug-like library)299.71183.75204
SGTC_3393091-483371.4 μM0.31253240077ChemDiv (Drug-like library)272.686463.1713iron homeostasis
SGTC_326k292-081217.5 μM0.3076923579357ChemDiv (Drug-like library)390.476524.2927
SGTC_13853091-483414.1 μM0.2923084072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion
SGTC_13993562-577225 μM0.28767123847490ChemDiv (Drug-like library)394.7829834.86305RPP1 & pyrimidine depletion
SGTC_13933470-564958.2 μM0.2857144108395ChemDiv (Drug-like library)283.3002632.3321460S ribosome export
SGTC_23307979373200 μM0.2769231863007Chembridge (Fragment library)247.316122.0241460S ribosome export
SGTC_21775811945192 μM0.2656255343475Chembridge (Fragment library)201.224540.91212
SGTC_5931203-021861.7 μM0.2615383666956ChemDiv (Drug-like library)244.33213.65302