9117578

6-methyl-2-(4-methylphenyl)-3,1-benzoxazin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3088
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 678341
SMILES CC1=CC=C(C=C1)C2=NC3=C(C=C(C=C3)C)C(=O)O2
Standardized SMILES Cc1ccc(cc1)C2=Nc3ccc(C)cc3C(=O)O2
Molecular weight 251.2799
ALogP 4.11
H-bond donor count 0
H-bond acceptor count 3
Response signature amide catabolism

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.72
% growth inhibition (Hom. pool) 1.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 678341
Download HIP data (tab-delimited text)  (excel)
Gene:BCP1(YDR361C)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.09||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC12(YHR107C)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.09||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:MCM7(YBR202W)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.02||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NTR2(YKR022C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:POP6(YGR030C)|FD-Score:-3.11|P-value:9.47E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP43(YGL120C)|FD-Score:4.63|P-value:1.82E-6|Clearance:0.85||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RHO1(YPR165W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.04||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL5(YPL131W)|FD-Score:-3.78|P-value:7.91E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEN15(YMR059W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.02||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SLY1(YDR189W)|FD-Score:3.1|P-value:9.78E-4|Clearance:0.02||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:UBA1(YKL210W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.21||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YKT6(YKL196C)|FD-Score:4.42|P-value:4.93E-6|Clearance:0.85||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:BCP1(YDR361C)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.09||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC12(YHR107C)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.09||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:MCM7(YBR202W)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.02||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NTR2(YKR022C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:POP6(YGR030C)|FD-Score:-3.11|P-value:9.47E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP43(YGL120C)|FD-Score:4.63|P-value:1.82E-6|Clearance:0.85||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RHO1(YPR165W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.04||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL5(YPL131W)|FD-Score:-3.78|P-value:7.91E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEN15(YMR059W)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.02||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SLY1(YDR189W)|FD-Score:3.1|P-value:9.78E-4|Clearance:0.02||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:UBA1(YKL210W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.21||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YKT6(YKL196C)|FD-Score:4.42|P-value:4.93E-6|Clearance:0.85||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 678341
Download HOP data (tab-delimited text)  (excel)
Gene:APJ1(YNL077W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:BAR1(YIL015W)|FD-Score:4.44|P-value:4.45E-6||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:CTT1(YGR088W)|FD-Score:-7.18|P-value:3.48E-13||SGD DESC:Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide Gene:DAL81(YIR023W)|FD-Score:7.17|P-value:3.83E-13||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUN1(YDL101C)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM7(YLR443W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EDC3(YEL015W)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:HPM1(YIL110W)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HYR1(YIR037W)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:IRC18(YJL037W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:MRPL38(YKL170W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:NPR2(YEL062W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:OPI1(YHL020C)|FD-Score:5.93|P-value:1.51E-9||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PCK1(YKR097W)|FD-Score:4.48|P-value:3.70E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PFA3(YNL326C)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:RHR2(YIL053W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RTC4(YNL254C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SAC3(YDR159W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SED1(YDR077W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SIL1(YOL031C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SOH1(YGL127C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SSA3(YBL075C)|FD-Score:3.73|P-value:9.58E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SYO1(YDL063C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TMA23(YMR269W)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRP3(YKL211C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:YCT1(YLL055W)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YGR050C(YGR050C_p)|FD-Score:5.37|P-value:4.00E-8||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YMR102C(YMR102C_p)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YMR265C(YMR265C_p)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Putative protein of unknown function Gene:YNL296W(YNL296W_d)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL038C-A(YOL038C-A_p)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YOS9(YDR057W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:APJ1(YNL077W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:BAR1(YIL015W)|FD-Score:4.44|P-value:4.45E-6||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:CTT1(YGR088W)|FD-Score:-7.18|P-value:3.48E-13||SGD DESC:Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide Gene:DAL81(YIR023W)|FD-Score:7.17|P-value:3.83E-13||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUN1(YDL101C)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM7(YLR443W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EDC3(YEL015W)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:HPM1(YIL110W)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HYR1(YIR037W)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:IRC18(YJL037W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:MRPL38(YKL170W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:NPR2(YEL062W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:OPI1(YHL020C)|FD-Score:5.93|P-value:1.51E-9||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PCK1(YKR097W)|FD-Score:4.48|P-value:3.70E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PFA3(YNL326C)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:RHR2(YIL053W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RTC4(YNL254C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SAC3(YDR159W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SED1(YDR077W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SIL1(YOL031C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SOH1(YGL127C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SSA3(YBL075C)|FD-Score:3.73|P-value:9.58E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SYO1(YDL063C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TMA23(YMR269W)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRP3(YKL211C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:YCT1(YLL055W)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YGR050C(YGR050C_p)|FD-Score:5.37|P-value:4.00E-8||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YMR102C(YMR102C_p)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YMR265C(YMR265C_p)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Putative protein of unknown function Gene:YNL296W(YNL296W_d)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL038C-A(YOL038C-A_p)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YOS9(YDR057W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL120C4.631.82E-60.85PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YKL196C4.424.93E-60.85YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YKR022C3.571.78E-40.00NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YKL210W3.571.80E-40.21UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YPR165W3.354.01E-40.04RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YDR361C3.324.57E-40.09BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YHR107C3.236.26E-40.09CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YBR202W3.148.52E-40.02MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YMR059W3.129.11E-40.02SEN15Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YDR189W3.109.78E-40.02SLY1Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
YLR145W3.080.001040.10RMP1Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
YPR181C2.980.001440.05SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YGR119C2.930.001680.05NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YOR370C2.880.002000.06MRS6Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
YPR082C2.820.002390.04DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W7.173.83E-13DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YHL020C5.931.51E-9OPI1Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
YGR050C_p5.374.00E-8YGR050C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YKR097W4.483.70E-6PCK1Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
YIL015W4.444.45E-6BAR1Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest
YDL101C4.434.74E-6DUN1Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair
YKL211C4.211.28E-5TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YOL038C-A_p3.875.55E-5YOL038C-A_pPutative protein of unknown function; identified by SAGE analysis
YMR265C_p3.816.92E-5YMR265C_pPutative protein of unknown function
YNL326C3.797.51E-5PFA3Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
YMR269W3.778.17E-5TMA23Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
YIR037W3.768.33E-5HYR1Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor
YBL075C3.739.58E-5SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
YKL170W3.611.52E-4MRPL38Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
YOL031C3.433.06E-4SIL1Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein

GO enrichment analysis for SGTC_3088
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2041.28E-56SGTC_486niguldipine 82.0 μMMiscellaneous12360.0777778amide catabolism
0.1903.11E-49SGTC_24605552655 174.2 μMMiscellaneous54019890.0806452amide catabolism
0.1842.47E-46SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.078125amide catabolism
0.1758.45E-42SGTC_10563448-1962 115.0 μMChemDiv (Drug-like library)7155070.101695amide catabolism
0.1714.08E-40SGTC_5694130-2812 160.0 μMChemDiv (Drug-like library)7435520.173077amide catabolism
0.1661.52E-37SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.09375amide catabolism
0.1653.23E-37SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.101695
0.1611.65E-35SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.173913amide catabolism
0.1584.13E-34SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.26
0.1431.72E-28SGTC_11811222-0449 220.0 μMChemDiv (Drug-like library)6583180.160714
0.1432.15E-28SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0666667
0.1315.97E-24SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.0641026
0.1292.22E-23SGTC_32289131799 49.5 μMChembridge (Drug-like library)170158180.153846amide catabolism
0.1283.94E-23SGTC_1819st053448 58.6 μMTimTec (Natural product derivative library)28576160.104478amide catabolism
0.1224.96E-21SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.09375

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1901st06021658.6 μM0.355556688792TimTec (Natural product derivative library)204.221882.5203
SGTC_15526-methylflavone10.6 μM0.340426689013TimTec (Pure natural product library)236.265283.62202superoxide
SGTC_511blebbistatin171 μM0.3214293476986ICCB bioactive library292.331842.42613DNA intercalators
SGTC_1732st03430851.82 μM0.319149830119TimTec (Natural product derivative library)277.531083.63602
SGTC_13853091-483414.1 μM0.3137264072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion
SGTC_20405100729123 μM0.30952411840Chembridge (Fragment library)161.157381.15612
SGTC_1907st06021083.7 μM0.306122688793TimTec (Natural product derivative library)204.221882.42203
SGTC_20124023501200 μM0.3023263477460Chembridge (Fragment library)175.183961.36202
SGTC_12670828-02729.49 μM0.3745334ChemDiv (Drug-like library)278.301963.72403
SGTC_1678st01926580.2 μM0.2916675367020TimTec (Natural product derivative library)249.264043.6080360S ribosome export