9117248

1-(3-chlorophenyl)-3-[3-(oxolan-2-ylmethoxy)phenyl]urea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3092
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17141857
SMILES C1CC(OC1)COC2=CC=CC(=C2)NC(=O)NC3=CC(=CC=C3)Cl
Standardized SMILES Clc1cccc(NC(=O)Nc2cccc(OCC3CCCO3)c2)c1
Molecular weight 346.8081
ALogP 3.53
H-bond donor count 2
H-bond acceptor count 3
Response signature cell wall signaling

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.06
% growth inhibition (Hom. pool) 1.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17141857
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.26||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:DED81(YHR019C)|FD-Score:4.34|P-value:7.17E-6|Clearance:0.95||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:GUS1(YGL245W)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.08||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:RHO1(YPR165W)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.95||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:TOP2(YNL088W)|FD-Score:-3.73|P-value:9.48E-5|Clearance:0||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:ACT1(YFL039C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.26||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:DED81(YHR019C)|FD-Score:4.34|P-value:7.17E-6|Clearance:0.95||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:GUS1(YGL245W)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.08||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:RHO1(YPR165W)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.95||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:TOP2(YNL088W)|FD-Score:-3.73|P-value:9.48E-5|Clearance:0||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17141857
Download HOP data (tab-delimited text)  (excel)
Gene:APS1(YLR170C)|FD-Score:3.83|P-value:6.54E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:BCK1(YJL095W)|FD-Score:6.47|P-value:5.04E-11||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CLB2(YPR119W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:FYV8(YGR196C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GET3(YDL100C)|FD-Score:-3.84|P-value:6.05E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GSM1(YJL103C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:KRE1(YNL322C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:PET20(YPL159C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:RHO5(YNL180C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:SLT2(YHR030C)|FD-Score:6.2|P-value:2.91E-10||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF7(YLR025W)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPS100(YHR139C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:YJL022W(YJL022W_d)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YNL034W(YNL034W_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YPR096C(YPR096C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:APS1(YLR170C)|FD-Score:3.83|P-value:6.54E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:BCK1(YJL095W)|FD-Score:6.47|P-value:5.04E-11||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CLB2(YPR119W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:FYV8(YGR196C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GET3(YDL100C)|FD-Score:-3.84|P-value:6.05E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GSM1(YJL103C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:KRE1(YNL322C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:PET20(YPL159C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:RHO5(YNL180C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:SLT2(YHR030C)|FD-Score:6.2|P-value:2.91E-10||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF7(YLR025W)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPS100(YHR139C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:YJL022W(YJL022W_d)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YNL034W(YNL034W_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YPR096C(YPR096C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR019C4.347.17E-60.95DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YPR165W4.241.10E-50.95RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YGL245W3.294.98E-40.08GUS1Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
YFL039C3.216.53E-40.26ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YDL043C2.960.001550.23PRP11Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YGL155W2.730.003160.08CDC43Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
YPR082C2.650.003980.12DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YKL104C2.540.005610.01GFA1Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
YKR022C2.520.005820.09NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YKL088W2.440.007440.02CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YEL019C2.410.007870.01MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YKL182W2.400.008120.01FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YDL132W2.400.008250.02CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YDR412W2.380.008670.01RRP17Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA
YPR181C2.370.008880.02SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL095W6.475.04E-11BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YHR030C6.202.91E-10SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YPR096C4.181.46E-5YPR096CProtein of unknown function that may interact with ribosomes, based on co-purification experiments
YJL022W_d4.102.09E-5YJL022W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
YLR025W3.934.26E-5SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YLR170C3.836.54E-5APS1Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
YJL103C3.601.58E-4GSM1Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis
YGR196C3.601.59E-4FYV8Protein of unknown function, required for survival upon exposure to K1 killer toxin
YNL180C3.492.45E-4RHO5Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity
YPR119W3.275.47E-4CLB2B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
YNL322C3.226.40E-4KRE1Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
YHR139C3.177.73E-4SPS100Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin
YNL034W_p3.109.70E-4YNL034W_pPutative protein of unknown function; YNL034W is not an essential gene
YFL019C_d3.080.00105YFL019C_dDubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene
YNL087W3.060.00110TCB2ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud

GO enrichment analysis for SGTC_3092
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4690SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.123288cell wall signaling
0.4154.43E-245SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.091954cell wall signaling
0.3916.14E-215SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.123077cell wall signaling
0.3831.50E-205SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.0952381cell wall signaling
0.3567.29E-176SGTC_20094012297 12.1 μMChembridge (Fragment library)455953070.0972222cell wall signaling
0.3501.49E-169SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.114286cell wall signaling
0.3433.74E-162SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.0958904cell wall signaling
0.3327.62E-152SGTC_31229126309 49.5 μMChembridge (Drug-like library)175912060.0714286cell wall signaling
0.3291.35E-148SGTC_422cantharidin 155.0 μMICCB bioactive library25450.0983607cell wall signaling
0.3148.71E-135SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.109375cell wall signaling
0.3044.10E-126SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0638298cell wall
0.3036.35E-126SGTC_500h-89 112.0 μMICCB bioactive library4492410.0909091cell wall signaling
0.3008.21E-123SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.102041cell wall signaling
0.2882.42E-113SGTC_408cantharidin 100.0 μMICCB bioactive library25450.0983607cell wall signaling
0.2862.05E-111SGTC_29249010168 62.8 μMChembridge (Drug-like library)29939290.197183cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3103912082249.47 μM0.63793117141819Chembridge (Drug-like library)327.374342.9414
SGTC_3226913125649.47 μM0.61666717178073Chembridge (Drug-like library)333.3292863.36815
SGTC_3072911641949.47 μM0.48387117170162Chembridge (Drug-like library)331.793423.62113ERG2
SGTC_3188911078949.47 μM0.448276849217Chembridge (Drug-like library)275.730163.61112iron homeostasis
SGTC_13742889-5571141 μM0.4333332853281ChemDiv (Drug-like library)269.724041.89613RSC complex & mRNA processing
SGTC_2867903909871.43 μM0.426236463460Chembridge (Drug-like library)287.740863.87912iron homeostasis
SGTC_12550566-0045221 μM0.4210532788469ChemDiv (Drug-like library)304.728323.04223excess fatty acid
SGTC_1918oxolan-2-ylmethyl 3-bromobenzoate70.1 μM0.416667551469TimTec (Natural product derivative library)285.133822.79203
SGTC_2900532852885.29 μM0.396552795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_2995906776771.43 μM0.37096811673567Chembridge (Drug-like library)280.773123.18222