9121669

6-(2-methylphenyl)-3-(2-methylpropyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3102
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 41905072
SMILES CC1=CC=CC=C1C2=NN3C(=NN=C3S2)CC(C)C
Standardized SMILES CC(C)Cc1nnc2sc(nn12)c3ccccc3C
Molecular weight 272.3686
ALogP 3.58
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.92
% growth inhibition (Hom. pool) 7.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 41905072
Download HIP data (tab-delimited text)  (excel)
Gene:CCA1(YER168C)|FD-Score:3.29|P-value:5.04E-4|Clearance:0.16||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:LSM3(YLR438C-A)|FD-Score:-3.34|P-value:4.18E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCM2(YBL023C)|FD-Score:5.13|P-value:1.46E-7|Clearance:0.93||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:PRP3(YDR473C)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.68||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRT1(YOR361C)|FD-Score:3.1|P-value:9.79E-4|Clearance:0.04||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:SEC21(YNL287W)|FD-Score:-3.15|P-value:8.10E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC31(YDL195W)|FD-Score:6.5|P-value:3.89E-11|Clearance:1.38||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMC2(YFR031C)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.08||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:SPC110(YDR356W)|FD-Score:3.13|P-value:8.83E-4|Clearance:0.03||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:UTP20(YBL004W)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.15||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:CCA1(YER168C)|FD-Score:3.29|P-value:5.04E-4|Clearance:0.16||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:LSM3(YLR438C-A)|FD-Score:-3.34|P-value:4.18E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCM2(YBL023C)|FD-Score:5.13|P-value:1.46E-7|Clearance:0.93||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:PRP3(YDR473C)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.68||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRT1(YOR361C)|FD-Score:3.1|P-value:9.79E-4|Clearance:0.04||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:SEC21(YNL287W)|FD-Score:-3.15|P-value:8.10E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC31(YDL195W)|FD-Score:6.5|P-value:3.89E-11|Clearance:1.38||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMC2(YFR031C)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.08||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:SPC110(YDR356W)|FD-Score:3.13|P-value:8.83E-4|Clearance:0.03||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:UTP20(YBL004W)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.15||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 41905072
Download HOP data (tab-delimited text)  (excel)
Gene:ARO2(YGL148W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG18(YFR021W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:BNI5(YNL166C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner Gene:BRR1(YPR057W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CDC50(YCR094W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CDC55(YGL190C)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:DAL81(YIR023W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCS1(YLR270W)|FD-Score:-3.95|P-value:3.83E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:FLO1(YAR050W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:HFD1(YMR110C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:HMO1(YDR174W)|FD-Score:-4.35|P-value:6.96E-6||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IRC25(YLR021W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:KIN4(YOR233W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LSM12(YHR121W)|FD-Score:4.54|P-value:2.78E-6||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MAL13(YGR288W)|FD-Score:6.48|P-value:4.59E-11||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MBP1(YDL056W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:OAR1(YKL055C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OCA4(YCR095C_p)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PAU7(YAR020C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PCL7(YIL050W)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PHO81(YGR233C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PMC1(YGL006W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:PPT2(YPL148C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD24(YER173W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RBK1(YCR036W)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Putative ribokinase Gene:RTG1(YOL067C)|FD-Score:4.95|P-value:3.67E-7||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SGF11(YPL047W)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SGF29(YCL010C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SQS1(YNL224C)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:TDA2(YER071C_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:VPS29(YHR012W)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:YGR079W(YGR079W_p)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YIL086C(YIL086C_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL016W(YJL016W_p)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YML020W(YML020W_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function Gene:YML089C(YML089C_d)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Putative protein of unknown function Gene:YOR318C(YOR318C_d)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:ARO2(YGL148W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG18(YFR021W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:BNI5(YNL166C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner Gene:BRR1(YPR057W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CDC50(YCR094W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CDC55(YGL190C)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:DAL81(YIR023W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCS1(YLR270W)|FD-Score:-3.95|P-value:3.83E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:FLO1(YAR050W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:HFD1(YMR110C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:HMO1(YDR174W)|FD-Score:-4.35|P-value:6.96E-6||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IRC25(YLR021W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:KIN4(YOR233W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LSM12(YHR121W)|FD-Score:4.54|P-value:2.78E-6||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MAL13(YGR288W)|FD-Score:6.48|P-value:4.59E-11||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MBP1(YDL056W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:OAR1(YKL055C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OCA4(YCR095C_p)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PAU7(YAR020C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PCL7(YIL050W)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PHO81(YGR233C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PMC1(YGL006W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:PPT2(YPL148C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD24(YER173W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RBK1(YCR036W)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Putative ribokinase Gene:RTG1(YOL067C)|FD-Score:4.95|P-value:3.67E-7||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SGF11(YPL047W)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SGF29(YCL010C)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SQS1(YNL224C)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:TDA2(YER071C_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:VPS29(YHR012W)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:YGR079W(YGR079W_p)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YIL086C(YIL086C_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL016W(YJL016W_p)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YML020W(YML020W_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function Gene:YML089C(YML089C_d)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Putative protein of unknown function Gene:YOR318C(YOR318C_d)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL195W6.503.89E-111.38SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YBL023C5.131.46E-70.93MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YFR031C4.201.36E-50.08SMC2Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
YDR473C4.121.89E-50.68PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YBL004W3.442.93E-40.15UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YER168C3.295.04E-40.16CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YDR356W3.138.83E-40.03SPC110Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
YOR361C3.109.79E-40.04PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YOR174W3.050.001140.01MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YBR152W3.040.001170.03SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YDR489W3.010.001310.10SLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YAL038W2.910.001790.02CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YMR061W2.890.001910.03RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YPR187W2.860.002110.06RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YHR062C2.800.002570.00RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR288W6.484.59E-11MAL13MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C
YOL067C4.953.67E-7RTG1Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus
YHR121W4.542.78E-6LSM12Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
YIL050W4.523.05E-6PCL7Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated
YPL047W4.356.69E-6SGF11Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
YAR020C4.151.65E-5PAU7Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
YPL148C4.141.75E-5PPT2Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YCR036W4.131.85E-5RBK1Putative ribokinase
YCR094W4.082.26E-5CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YIR023W3.934.18E-5DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YNL097C-B_p3.895.09E-5YNL097C-B_pPutative protein of unknown function
YNL224C3.836.32E-5SQS1Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress
YGR233C3.797.48E-5PHO81Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress
YMR001C-A_p3.601.59E-4YMR001C-A_pPutative protein of unknown function
YGL148W3.452.85E-4ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_3102
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1771.67E-42SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.0481928
0.1584.08E-34SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.0625
0.1263.16E-22SGTC_31109122132 49.5 μMChembridge (Drug-like library)9183170.106061redox potentiating
0.1241.12E-21SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.114286
0.1111.12E-17SGTC_525k781-2675 26.7 μMChemDiv (Drug-like library)36511180.0909091redox potentiating
0.1055.50E-16SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0735294
0.1032.70E-15SGTC_31979113331 49.5 μMChembridge (Drug-like library)166104590.125
0.1024.66E-15SGTC_12680828-0275 2.5 μMChemDiv (Drug-like library)7453370.107692
0.0978.49E-14SGTC_12670828-0272 9.5 μMChemDiv (Drug-like library)7453340.0909091
0.0961.72E-13SGTC_1260417-1624 672.5 μMChemDiv (Drug-like library)419120.0746269DNA intercalators
0.0945.41E-13SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0985916
0.0921.18E-12SGTC_24605552655 174.2 μMMiscellaneous54019890.0882353amide catabolism
0.0921.36E-12SGTC_486niguldipine 82.0 μMMiscellaneous12360.0833333amide catabolism
0.0896.00E-12SGTC_5694130-2812 160.0 μMChemDiv (Drug-like library)7435520.0967742amide catabolism
0.0855.66E-11SGTC_29107948405 26.0 μMChembridge (Drug-like library)29713280.0731707plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3292912082559.78 μM0.7524824900Chembridge (Drug-like library)276.3325233.29405
SGTC_3307912688569.28 μM0.67391324824909Chembridge (Drug-like library)272.368643.77904
SGTC_3073911497249.47 μM0.62541905075Chembridge (Drug-like library)272.368643.5750460S ribosome export
SGTC_3306912767659.79 μM0.61702141905287Chembridge (Drug-like library)264.369783.04205
SGTC_3299912198243.13 μM0.59183717594604Chembridge (Drug-like library)273.35672.22105
SGTC_3300912461550.48 μM0.57142924824903Chembridge (Drug-like library)288.368043.07205
SGTC_3301912535419.8 μM0.55769241905162Chembridge (Drug-like library)301.409863.25105
SGTC_3302912539516.35 μM0.5417599668Chembridge (Drug-like library)284.379343.55704
SGTC_3116912494349.47 μM0.52941241905195Chembridge (Drug-like library)288.368042.92405
SGTC_3305912702753.83 μM0.51851941905295Chembridge (Drug-like library)316.378142.87506