9121286

N-(2,4-dimethylphenyl)-3-(3,4,5-trimethylpyrazol-1-yl)butanamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3106
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 15943471
SMILES CC1=CC(=C(C=C1)NC(=O)CC(C)N2C(=C(C(=N2)C)C)C)C
Standardized SMILES CC(CC(=O)Nc1ccc(C)cc1C)n2nc(C)c(C)c2C
Molecular weight 299.4106
ALogP 3.63
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.07
% growth inhibition (Hom. pool) 2.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 15943471
Download HIP data (tab-delimited text)  (excel)
Gene:CCT6(YDR188W)|FD-Score:-3.36|P-value:3.96E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC25(YLR310C)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DRS1(YLL008W)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.22||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:GAB1(YLR459W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:HEM1(YDR232W)|FD-Score:3.13|P-value:8.79E-4|Clearance:0.21||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:LSM3(YLR438C-A)|FD-Score:-3.72|P-value:9.84E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MMS21(YEL019C)|FD-Score:-3.92|P-value:4.38E-5|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NUP57(YGR119C)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:POP8(YBL018C)|FD-Score:-3.58|P-value:1.74E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PWP2(YCR057C)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.02||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:PZF1(YPR186C)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.03||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RLP24(YLR009W)|FD-Score:4.38|P-value:5.91E-6|Clearance:0.36||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC82(YPR190C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.17||SGD DESC:RNA polymerase III subunit C82 Gene:RPL15A(YLR029C)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:SMD1(YGR074W)|FD-Score:3.9|P-value:4.91E-5|Clearance:0.21||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SNU23(YDL098C)|FD-Score:-4.22|P-value:1.22E-5|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPB4(YFL002C)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.36||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFB1(YDR311W)|FD-Score:-3.1|P-value:9.55E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TID3(YIL144W)|FD-Score:-4.89|P-value:5.07E-7|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UTP4(YDR324C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YDL196W(YDL196W_d)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL015C(YJL015C_d)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:CCT6(YDR188W)|FD-Score:-3.36|P-value:3.96E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC25(YLR310C)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DRS1(YLL008W)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.22||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:GAB1(YLR459W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:HEM1(YDR232W)|FD-Score:3.13|P-value:8.79E-4|Clearance:0.21||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:LSM3(YLR438C-A)|FD-Score:-3.72|P-value:9.84E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MMS21(YEL019C)|FD-Score:-3.92|P-value:4.38E-5|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NUP57(YGR119C)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:POP8(YBL018C)|FD-Score:-3.58|P-value:1.74E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PWP2(YCR057C)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.02||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:PZF1(YPR186C)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.03||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RLP24(YLR009W)|FD-Score:4.38|P-value:5.91E-6|Clearance:0.36||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC82(YPR190C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.17||SGD DESC:RNA polymerase III subunit C82 Gene:RPL15A(YLR029C)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:SMD1(YGR074W)|FD-Score:3.9|P-value:4.91E-5|Clearance:0.21||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SNU23(YDL098C)|FD-Score:-4.22|P-value:1.22E-5|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPB4(YFL002C)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.36||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFB1(YDR311W)|FD-Score:-3.1|P-value:9.55E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TID3(YIL144W)|FD-Score:-4.89|P-value:5.07E-7|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UTP4(YDR324C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YDL196W(YDL196W_d)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL015C(YJL015C_d)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 15943471
Download HOP data (tab-delimited text)  (excel)
Gene:AHP1(YLR109W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:AIR2(YDL175C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ATP7(YKL016C)|FD-Score:6.24|P-value:2.13E-10||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BDS1(YOL164W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BNA4(YBL098W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CBP1(YJL209W)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CNB1(YKL190W)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COX12(YLR038C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CTL1(YMR180C)|FD-Score:-4.52|P-value:3.09E-6||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:DIA3(YDL024C)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DIA4(YHR011W)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DUF1(YOL087C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EMI1(YDR512C)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERI1(YPL096C-A)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FBP1(YLR377C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:GBP2(YCL011C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GTR1(YML121W)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HAP2(YGL237C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HLJ1(YMR161W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:ICY2(YPL250C)|FD-Score:4.64|P-value:1.73E-6||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:INO4(YOL108C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LRG1(YDL240W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MDM35(YKL053C-A)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRPS17(YMR188C)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NOT3(YIL038C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPL3(YDR432W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NUM1(YDR150W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OMS1(YDR316W)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PBP4(YDL053C)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PLB1(YMR008C)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PRS1(YKL181W)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RAD55(YDR076W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RPL34B(YIL052C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.46|P-value:4.08E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT13(YER066W_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RTG1(YOL067C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SAC6(YDR129C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SED1(YDR077W)|FD-Score:-3.91|P-value:4.54E-5||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SGF11(YPL047W)|FD-Score:5.6|P-value:1.09E-8||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SHU2(YDR078C)|FD-Score:-3.99|P-value:3.34E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SKN1(YGR143W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SNF7(YLR025W)|FD-Score:-3.72|P-value:9.98E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNT309(YPR101W)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPC1(YJR010C-A)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPL2(YHR136C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SRN2(YLR119W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:TOF2(YKR010C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TPS2(YDR074W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URA4(YLR420W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VPH1(YOR270C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCK1(YHR135C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YDL034W(YDL034W_d)|FD-Score:-4.34|P-value:7.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDL068W(YDL068W_d)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR114C(YDR114C_p)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YER091C-A(YER091C-A_d)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR012W-A(YFR012W-A_p)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGR111W(YGR111W_p)|FD-Score:-3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR237C(YGR237C_p)|FD-Score:-6.89|P-value:2.80E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YJL182C(YJL182C_d)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YKL070W(YKL070W_p)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL118W(YKL118W_d)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR041W(YLR041W_d)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Gene:YNL109W(YNL109W_d)|FD-Score:5.93|P-value:1.55E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YNR071C(YNR071C_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative aldose 1-epimerase Gene:YOL050C(YOL050C_d)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPT31(YER031C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:AHP1(YLR109W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:AIR2(YDL175C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ATP7(YKL016C)|FD-Score:6.24|P-value:2.13E-10||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BDS1(YOL164W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BNA4(YBL098W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CBP1(YJL209W)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CNB1(YKL190W)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COX12(YLR038C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CTL1(YMR180C)|FD-Score:-4.52|P-value:3.09E-6||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:DIA3(YDL024C)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DIA4(YHR011W)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DUF1(YOL087C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EMI1(YDR512C)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERI1(YPL096C-A)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FBP1(YLR377C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:GBP2(YCL011C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GTR1(YML121W)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HAP2(YGL237C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HLJ1(YMR161W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:ICY2(YPL250C)|FD-Score:4.64|P-value:1.73E-6||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:INO4(YOL108C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LRG1(YDL240W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MDM35(YKL053C-A)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRPS17(YMR188C)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NOT3(YIL038C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPL3(YDR432W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NUM1(YDR150W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OMS1(YDR316W)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PBP4(YDL053C)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PLB1(YMR008C)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PRS1(YKL181W)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RAD55(YDR076W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RPL34B(YIL052C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.46|P-value:4.08E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT13(YER066W_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RTG1(YOL067C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SAC6(YDR129C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SED1(YDR077W)|FD-Score:-3.91|P-value:4.54E-5||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SGF11(YPL047W)|FD-Score:5.6|P-value:1.09E-8||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SHU2(YDR078C)|FD-Score:-3.99|P-value:3.34E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SKN1(YGR143W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SNF7(YLR025W)|FD-Score:-3.72|P-value:9.98E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNT309(YPR101W)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPC1(YJR010C-A)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPL2(YHR136C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SRN2(YLR119W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:TOF2(YKR010C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TPS2(YDR074W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URA4(YLR420W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VPH1(YOR270C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCK1(YHR135C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YDL034W(YDL034W_d)|FD-Score:-4.34|P-value:7.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDL068W(YDL068W_d)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR114C(YDR114C_p)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YER091C-A(YER091C-A_d)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR012W-A(YFR012W-A_p)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGR111W(YGR111W_p)|FD-Score:-3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR237C(YGR237C_p)|FD-Score:-6.89|P-value:2.80E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YJL182C(YJL182C_d)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YKL070W(YKL070W_p)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL118W(YKL118W_d)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR041W(YLR041W_d)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Gene:YNL109W(YNL109W_d)|FD-Score:5.93|P-value:1.55E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YNR071C(YNR071C_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative aldose 1-epimerase Gene:YOL050C(YOL050C_d)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPT31(YER031C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR009W4.385.91E-60.36RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YFL002C4.251.05E-50.36SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YGR074W3.904.91E-50.21SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YLR029C3.681.16E-40.02RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YPR190C3.661.27E-40.17RPC82RNA polymerase III subunit C82
YJL015C_d3.492.44E-40.05YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YPR186C3.442.96E-40.03PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YCR057C3.413.30E-40.02PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YGR119C3.383.57E-40.01NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YLL008W3.383.66E-40.22DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
YDL196W_d3.167.87E-40.03YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YDR232W3.138.79E-40.21HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YLR005W2.920.001760.03SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YPL169C2.890.001910.01MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YCR093W2.880.001970.05CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL016C6.242.13E-10ATP7Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YNL109W_d5.931.55E-9YNL109W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C
YPL047W5.601.09E-8SGF11Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
YBR046C4.808.13E-7ZTA1NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin
YPL250C4.641.73E-6ICY2Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate
YJL182C_d4.582.30E-6YJL182C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W
YLR041W_d4.171.52E-5YLR041W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C
YOL050C_d4.161.61E-5YOL050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YNR071C_p4.022.89E-5YNR071C_pPutative aldose 1-epimerase
YIL038C3.983.44E-5NOT3Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
YKL053C-A3.973.67E-5MDM35Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
YDL240W3.934.33E-5LRG1Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis
YDR114C_p3.904.74E-5YDR114C_pPutative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium
YHR011W3.865.64E-5DIA4Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth
YPR101W3.836.42E-5SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p

GO enrichment analysis for SGTC_3106
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1503.56E-31SGTC_31179124945 49.5 μMChembridge (Drug-like library)175920650.131579
0.0781.67E-9SGTC_22216807166 181.1 μMChembridge (Fragment library)6749250.0833333
0.0672.36E-7SGTC_32389133470 49.5 μMChembridge (Drug-like library)35346880.196721
0.0672.95E-7SGTC_30279089541 49.5 μMChembridge (Drug-like library)99438190.115942fatty acid desaturase (OLE1)
0.0604.01E-6SGTC_32049114183 49.5 μMChembridge (Drug-like library)170272480.147059
0.0595.91E-6SGTC_22777947813 106.9 μMChembridge (Fragment library)12467980.184615RSC complex & mRNA processing
0.0588.23E-6SGTC_1468k284-1086 134.0 μMChemDiv (Drug-like library)159964570.0886076DNA intercalators
0.0579.99E-6SGTC_20155119600 184.0 μMChembridge (Fragment library)28287860.0714286
0.0571.18E-5SGTC_11293453-1384 223.0 μMChemDiv (Drug-like library)10844520.0789474
0.0571.38E-5SGTC_8093998-0021 90.0 μMChemDiv (Drug-like library)7154850.0945946RNA processing & uracil transport
0.0552.38E-5SGTC_262fenpropimorph 62.5 μMMiscellaneous916950.0985916ergosterol depletion effects on membrane
0.0534.14E-5SGTC_31149122766 49.5 μMChembridge (Drug-like library)171769850.12987
0.0534.40E-5SGTC_31029121669 49.5 μMChembridge (Drug-like library)419050720.176471
0.0534.61E-5SGTC_2515chrysin 20.8 μMMicrosource (Natural product library)52816070.0714286RPP1 & pyrimidine depletion
0.0526.73E-5SGTC_28419004761 39.0 μMChembridge (Drug-like library)29910390.246154amide catabolism

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2709st07856056 μM0.35211317250903TimTec (Natural product derivative library)357.430062.43724
SGTC_9671306-0166352 μM0.2950824437607ChemDiv (Drug-like library)246.265163.37524
SGTC_2838900268771.43 μM0.2878796457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_2876905990325.97 μM0.2857146916095Chembridge (Drug-like library)283.364843.91312
SGTC_2893904848825.97 μM0.2857146467338Chembridge (Drug-like library)337.458583.46913fatty acid desaturase (OLE1)
SGTC_21175521686200 μM0.283333803312Chembridge (Fragment library)227.261821.71413
SGTC_2856902292519.48 μM0.281254944862Chembridge (Drug-like library)287.3287234.11213
SGTC_2246729350791.56 μM0.275862887656Chembridge (Fragment library)244.245980.99413
SGTC_23916285546200 μM0.271186846083Chembridge (Fragment library)208.644262.1612
SGTC_3228913179949.47 μM0.26470617015818Chembridge (Drug-like library)307.349783.15224amide catabolism