9123419

N-cyclohexyl-N-phenylfuran-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3112
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17015854
SMILES C1CCC(CC1)N(C2=CC=CC=C2)C(=O)C3=COC=C3
Standardized SMILES O=C(N(C1CCCCC1)c2ccccc2)c3cocc3
Molecular weight 269.3383
ALogP 3.78
H-bond donor count 0
H-bond acceptor count 2
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.44
% growth inhibition (Hom. pool) 5.5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17015854
Download HIP data (tab-delimited text)  (excel)
Gene:CAK1(YFL029C)|FD-Score:-4.68|P-value:1.43E-6|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT5(YJR064W)|FD-Score:-5.12|P-value:1.55E-7|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CWC2(YDL209C)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.47||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:EFB1(YAL003W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.2||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:GLN4(YOR168W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.02||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:LSM2(YBL026W)|FD-Score:4.31|P-value:8.34E-6|Clearance:0.11||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NSP1(YJL041W)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PGI1(YBR196C)|FD-Score:5.37|P-value:3.93E-8|Clearance:0.65||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RFC3(YNL290W)|FD-Score:-3.24|P-value:6.06E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPP1(YHR062C)|FD-Score:4.97|P-value:3.37E-7|Clearance:0.65||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT1(YKL145W)|FD-Score:3.4|P-value:3.36E-4|Clearance:0.19||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SEC31(YDL195W)|FD-Score:4.95|P-value:3.64E-7|Clearance:0.65||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC34(YKR037C)|FD-Score:3.72|P-value:9.85E-5|Clearance:0.1||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SUI3(YPL237W)|FD-Score:3.1|P-value:9.62E-4|Clearance:0.02||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TOM22(YNL131W)|FD-Score:3.21|P-value:6.58E-4|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:UBC1(YDR177W)|FD-Score:3.14|P-value:8.46E-4|Clearance:0.04||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YDR526C(YDR526C_d)|FD-Score:5.19|P-value:1.04E-7|Clearance:0.65||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR190C(YGR190C_d)|FD-Score:-5.18|P-value:1.13E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YLR317W(YLR317W_d)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:CAK1(YFL029C)|FD-Score:-4.68|P-value:1.43E-6|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT5(YJR064W)|FD-Score:-5.12|P-value:1.55E-7|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CWC2(YDL209C)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.47||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:EFB1(YAL003W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.2||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:GLN4(YOR168W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.02||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:LSM2(YBL026W)|FD-Score:4.31|P-value:8.34E-6|Clearance:0.11||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NSP1(YJL041W)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PGI1(YBR196C)|FD-Score:5.37|P-value:3.93E-8|Clearance:0.65||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RFC3(YNL290W)|FD-Score:-3.24|P-value:6.06E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPP1(YHR062C)|FD-Score:4.97|P-value:3.37E-7|Clearance:0.65||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT1(YKL145W)|FD-Score:3.4|P-value:3.36E-4|Clearance:0.19||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:SEC31(YDL195W)|FD-Score:4.95|P-value:3.64E-7|Clearance:0.65||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC34(YKR037C)|FD-Score:3.72|P-value:9.85E-5|Clearance:0.1||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SUI3(YPL237W)|FD-Score:3.1|P-value:9.62E-4|Clearance:0.02||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TOM22(YNL131W)|FD-Score:3.21|P-value:6.58E-4|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:UBC1(YDR177W)|FD-Score:3.14|P-value:8.46E-4|Clearance:0.04||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YDR526C(YDR526C_d)|FD-Score:5.19|P-value:1.04E-7|Clearance:0.65||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR190C(YGR190C_d)|FD-Score:-5.18|P-value:1.13E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YLR317W(YLR317W_d)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17015854
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG18(YFR021W)|FD-Score:-5.6|P-value:1.09E-8||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP7(YKL016C)|FD-Score:6|P-value:9.95E-10||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BDF2(YDL070W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:CDA2(YLR308W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDC26(YFR036W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CTR1(YPR124W)|FD-Score:4.05|P-value:2.62E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DLD2(YDL178W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:ECT1(YGR007W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:EPS1(YIL005W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FMP27(YLR454W_p)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FTR1(YER145C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GLO1(YML004C)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GLY1(YEL046C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GTR2(YGR163W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HCR1(YLR192C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:ICY2(YPL250C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IRC7(YFR055W_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:LSM1(YJL124C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MAC1(YMR021C)|FD-Score:5.29|P-value:5.96E-8||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCM16(YPR046W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MCT1(YOR221C)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MEP1(YGR121C)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET12(YPL023C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MNL1(YHR204W)|FD-Score:4.47|P-value:3.92E-6||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MPD2(YOL088C)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRP8(YKL142W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MRPL51(YPR100W)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSI1(YBR195C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NGG1(YDR176W)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PAF1(YBR279W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PCL7(YIL050W)|FD-Score:-4.31|P-value:8.04E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PKR1(YMR123W)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PYC2(YBR218C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAI1(YGL246C)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RPL20A(YMR242C)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RXT2(YBR095C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SET5(YHR207C)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SIS2(YKR072C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLS1(YLR139C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SOD1(YJR104C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SWI4(YER111C)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYH1(YPL105C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TIF4631(YGR162W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TPS2(YDR074W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:UFD4(YKL010C)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:VMA13(YPR036W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS29(YHR012W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS36(YLR417W)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR013C(YBR013C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR226C(YBR226C_d)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YER084W(YER084W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL025C(YIL025C_d)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR018W(YJR018W_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR294C(YLR294C_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YOR364W(YOR364W_d)|FD-Score:-3.77|P-value:8.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPL039W(YPL039W_p)|FD-Score:-6.23|P-value:2.29E-10||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YTP1(YNL237W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:AFT1(YGL071W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG18(YFR021W)|FD-Score:-5.6|P-value:1.09E-8||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP7(YKL016C)|FD-Score:6|P-value:9.95E-10||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BDF2(YDL070W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:CDA2(YLR308W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDC26(YFR036W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CTR1(YPR124W)|FD-Score:4.05|P-value:2.62E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DLD2(YDL178W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:ECT1(YGR007W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:EPS1(YIL005W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FMP27(YLR454W_p)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FTR1(YER145C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GLO1(YML004C)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GLY1(YEL046C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GTR2(YGR163W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HCR1(YLR192C)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:ICY2(YPL250C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IRC7(YFR055W_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:LSM1(YJL124C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MAC1(YMR021C)|FD-Score:5.29|P-value:5.96E-8||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCM16(YPR046W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MCT1(YOR221C)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MEP1(YGR121C)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET12(YPL023C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MNL1(YHR204W)|FD-Score:4.47|P-value:3.92E-6||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MPD2(YOL088C)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRP8(YKL142W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MRPL51(YPR100W)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSI1(YBR195C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NGG1(YDR176W)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PAF1(YBR279W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PCL7(YIL050W)|FD-Score:-4.31|P-value:8.04E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PKR1(YMR123W)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PYC2(YBR218C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAI1(YGL246C)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RPL20A(YMR242C)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RXT2(YBR095C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SET5(YHR207C)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SIS2(YKR072C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLS1(YLR139C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SOD1(YJR104C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SWI4(YER111C)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYH1(YPL105C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TIF4631(YGR162W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TPS2(YDR074W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:UFD4(YKL010C)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:VMA13(YPR036W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS29(YHR012W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS36(YLR417W)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR013C(YBR013C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR226C(YBR226C_d)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YER084W(YER084W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL025C(YIL025C_d)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR018W(YJR018W_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR294C(YLR294C_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YOR364W(YOR364W_d)|FD-Score:-3.77|P-value:8.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPL039W(YPL039W_p)|FD-Score:-6.23|P-value:2.29E-10||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YTP1(YNL237W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR196C5.373.93E-80.65PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YDR526C_d5.191.04E-70.65YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHR062C4.973.37E-70.65RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDL195W4.953.64E-70.65SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YBL026W4.318.34E-60.11LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YDL209C4.201.36E-50.47CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YKR037C3.729.85E-50.10SPC34Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YOR168W3.621.45E-40.02GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YAL003W3.601.58E-40.20EFB1Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
YKL145W3.403.36E-40.19RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YNL131W3.216.58E-40.07TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YDR177W3.148.46E-40.04UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YPL237W3.109.62E-40.02SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YKL153W_d3.090.001010.10YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YLR163C2.990.001390.03MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL016C6.009.95E-10ATP7Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YMR021C5.295.96E-8MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YPR100W4.856.17E-7MRPL51Mitochondrial ribosomal protein of the large subunit
YHR204W4.473.92E-6MNL1Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation
YLR417W4.337.43E-6VPS36Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
YIL025C_d4.151.68E-5YIL025C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL105C4.141.75E-5SYH1Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
YBR279W4.121.92E-5PAF1Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
YPR124W4.052.62E-5CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YBR226C_d4.022.88E-5YBR226C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W
YHR207C3.973.67E-5SET5Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
YMR123W3.934.30E-5PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YOR221C3.904.80E-5MCT1Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
YMR242C3.894.98E-5RPL20ARibosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
YPR123C_d3.895.07E-5YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

GO enrichment analysis for SGTC_3112
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1382.42E-26SGTC_1060092-0003 11.6 μMChemDiv (Drug-like library)10721480.135593copper-dependent oxidative stress
0.1217.69E-21SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.0566038copper-dependent oxidative stress
0.1211.24E-20SGTC_3620310-0100 9.0 μMChemDiv (Drug-like library)7902350.136364RPP1 & pyrimidine depletion
0.1171.87E-19SGTC_30359091303 49.5 μMChembridge (Drug-like library)252370020.16129
0.1165.81E-19SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.118421
0.1141.77E-18SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.111111copper-dependent oxidative stress
0.1133.02E-18SGTC_524r073-0010 27.8 μMChemDiv (Drug-like library)27721060.0724638
0.1111.03E-17SGTC_1959st077091 55.8 μMTimTec (Natural product derivative library)8213560.123077RPP1 & pyrimidine depletion
0.1101.92E-17SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0757576
0.1102.96E-17SGTC_1964st076914 61.0 μMTimTec (Natural product derivative library)242086610.125RPP1 & pyrimidine depletion
0.0971.06E-13SGTC_1629st005085 44.9 μMTimTec (Natural product derivative library)242070910.21875RPP1 & pyrimidine depletion
0.0952.08E-13SGTC_10014048-4355 44.4 μMChemDiv (Drug-like library)218242330.112676copper-dependent oxidative stress
0.0952.11E-13SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.117647
0.0953.36E-13SGTC_20805212524 12.4 μMChembridge (Fragment library)2601830.111111copper-dependent oxidative stress
0.0943.71E-13SGTC_1000086-0128 49.6 μMChemDiv (Drug-like library)932970.0606061copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22619010538184.68 μM0.366457250Chembridge (Fragment library)233.306162.8612
SGTC_3162909965349.47 μM0.26315825237511Chembridge (Drug-like library)300.438423.56802
SGTC_12780874-0585172 μM0.254902766306ChemDiv (Drug-like library)234.360424.3721
SGTC_1837st05547266.5 μM0.25676091TimTec (Natural product derivative library)301.380123.68703
SGTC_2955907625412.21 μM0.2516459619Chembridge (Drug-like library)316.394762.72414
SGTC_20625245942200 μM0.2407411376160Chembridge (Fragment library)215.247822.22202
SGTC_13351486-0900202 μM0.238806X1335ChemDiv (Drug-like library)395.516254.1542
SGTC_7131488-1073324 μM0.2380953312542ChemDiv (Drug-like library)493.596284.48926ERG2
SGTC_1628st00425277.7 μM0.237288692968TimTec (Natural product derivative library)257.28452.4713
SGTC_2350902389538.85 μM0.2321432997209Chembridge (Fragment library)266.337622.29402ergosterol biosynthesis