9123498

2-(3,4-dihydro-1H-isoquinolin-2-yl)-N-(2-propan-2-ylphenyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3113
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 8897275
SMILES CC(C)C1=CC=CC=C1NC(=O)CN2CCC3=CC=CC=C3C2
Standardized SMILES CC(C)c1ccccc1NC(=O)CN2CCc3ccccc3C2
Molecular weight 308.4174
ALogP 3.81
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.95
% growth inhibition (Hom. pool) 3.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 8897275
Download HIP data (tab-delimited text)  (excel)
Gene:CCT8(YJL008C)|FD-Score:3.87|P-value:5.37E-5|Clearance:0.16||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CWC24(YLR323C)|FD-Score:-3.23|P-value:6.20E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:GCR1(YPL075W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.07||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:LSG1(YGL099W)|FD-Score:-3.81|P-value:7.07E-5|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM2(YBL026W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.12||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:4.97|P-value:3.27E-7|Clearance:0.61||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MEC1(YBR136W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.01||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:RNA14(YMR061W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.12||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPL5(YPL131W)|FD-Score:7.24|P-value:2.28E-13|Clearance:2.26||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:4.29|P-value:8.82E-6|Clearance:0.42||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS31(YLR167W)|FD-Score:4.36|P-value:6.43E-6|Clearance:0.07||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SPN1(YPR133C)|FD-Score:3.18|P-value:7.44E-4|Clearance:0.05||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAF12(YDR145W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.04||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TOP2(YNL088W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.03||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:TSC11(YER093C)|FD-Score:-3.8|P-value:7.21E-5|Clearance:0||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:YDL016C(YDL016C_d)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:CCT8(YJL008C)|FD-Score:3.87|P-value:5.37E-5|Clearance:0.16||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CWC24(YLR323C)|FD-Score:-3.23|P-value:6.20E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:GCR1(YPL075W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.07||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:LSG1(YGL099W)|FD-Score:-3.81|P-value:7.07E-5|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM2(YBL026W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.12||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:4.97|P-value:3.27E-7|Clearance:0.61||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MEC1(YBR136W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.01||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:RNA14(YMR061W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.12||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPL5(YPL131W)|FD-Score:7.24|P-value:2.28E-13|Clearance:2.26||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:4.29|P-value:8.82E-6|Clearance:0.42||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS31(YLR167W)|FD-Score:4.36|P-value:6.43E-6|Clearance:0.07||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SPN1(YPR133C)|FD-Score:3.18|P-value:7.44E-4|Clearance:0.05||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAF12(YDR145W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.04||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TOP2(YNL088W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.03||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:TSC11(YER093C)|FD-Score:-3.8|P-value:7.21E-5|Clearance:0||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:YDL016C(YDL016C_d)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 8897275
Download HOP data (tab-delimited text)  (excel)
Gene:ATG18(YFR021W)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP7(YKL016C)|FD-Score:4.99|P-value:3.05E-7||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:COG6(YNL041C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTM1(YHR109W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CUE5(YOR042W)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DEG1(YFL001W)|FD-Score:4.32|P-value:7.93E-6||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DLD2(YDL178W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:ERG2(YMR202W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:MDM20(YOL076W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MGT1(YDL200C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:ODC1(YPL134C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:PAP2(YOL115W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PCI8(YIL071C)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEP7(YDR323C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PFA5(YDR459C)|FD-Score:-3.85|P-value:5.97E-5||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PHO89(YBR296C)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:POS5(YPL188W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:RPN4(YDL020C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SCW4(YGR279C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SEC72(YLR292C)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SET4(YJL105W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SHB17(YKR043C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHE10(YGL228W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SIP18(YMR175W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SMI1(YGR229C)|FD-Score:5.17|P-value:1.14E-7||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF4(YGL115W)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNF8(YPL002C)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:TRS65(YGR166W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:UGA2(YBR006W)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS27(YNR006W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS61(YDR136C_d)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS69(YPR087W_d)|FD-Score:5.38|P-value:3.69E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YDR161W(YDR161W_p)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YJL045W(YJL045W)|FD-Score:-4.04|P-value:2.65E-5||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YLR278C(YLR278C_p)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YLR460C(YLR460C_p)|FD-Score:5.46|P-value:2.40E-8||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR086C-A(YMR086C-A_d)|FD-Score:-3.87|P-value:5.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR087W(YMR087W)|FD-Score:4.81|P-value:7.37E-7||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNR025C(YNR025C_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YRO2(YBR054W)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ATG18(YFR021W)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP7(YKL016C)|FD-Score:4.99|P-value:3.05E-7||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:COG6(YNL041C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTM1(YHR109W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CUE5(YOR042W)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DEG1(YFL001W)|FD-Score:4.32|P-value:7.93E-6||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DLD2(YDL178W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:ERG2(YMR202W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:MDM20(YOL076W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MGT1(YDL200C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:ODC1(YPL134C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:PAP2(YOL115W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PCI8(YIL071C)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEP7(YDR323C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PFA5(YDR459C)|FD-Score:-3.85|P-value:5.97E-5||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PHO89(YBR296C)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:POS5(YPL188W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:RPN4(YDL020C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SCW4(YGR279C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SEC72(YLR292C)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SET4(YJL105W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SHB17(YKR043C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHE10(YGL228W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SIP18(YMR175W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SMI1(YGR229C)|FD-Score:5.17|P-value:1.14E-7||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF4(YGL115W)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNF8(YPL002C)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:TRS65(YGR166W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:UGA2(YBR006W)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS27(YNR006W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS61(YDR136C_d)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS69(YPR087W_d)|FD-Score:5.38|P-value:3.69E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YDR161W(YDR161W_p)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YJL045W(YJL045W)|FD-Score:-4.04|P-value:2.65E-5||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YLR278C(YLR278C_p)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YLR460C(YLR460C_p)|FD-Score:5.46|P-value:2.40E-8||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR086C-A(YMR086C-A_d)|FD-Score:-3.87|P-value:5.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR087W(YMR087W)|FD-Score:4.81|P-value:7.37E-7||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNR025C(YNR025C_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YRO2(YBR054W)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL131W7.242.28E-132.26RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YLR163C4.973.27E-70.61MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YLR167W4.366.43E-60.07RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YHR062C4.298.82E-60.42RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YJL008C3.875.37E-50.16CCT8Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR064W3.711.02E-40.02RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YBR136W3.701.09E-40.01MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YPL075W3.691.12E-40.07GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YMR061W3.621.49E-40.12RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YNL088W3.502.35E-40.03TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YDL016C_d3.462.67E-40.16YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YBL026W3.304.83E-40.12LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YPR133C3.187.44E-40.05SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YDR145W3.138.86E-40.04TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YDR280W3.090.001010.04RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR460C_p5.462.40E-8YLR460C_pMember of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine
YPR087W_d5.383.69E-8VPS69_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect
YGR229C5.171.14E-7SMI1Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
YKL016C4.993.05E-7ATP7Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YMR087W4.817.37E-7YMR087WPutative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain
YPL002C4.711.27E-6SNF8Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
YBR006W4.552.63E-6UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YDR136C_d4.405.34E-6VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YFL001W4.327.93E-6DEG1tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
YIL071C4.032.83E-5PCI8Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
YGL115W3.953.83E-5SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YNR006W3.904.78E-5VPS27Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
YOR042W3.739.67E-5CUE5Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YOL115W3.711.03E-4PAP2Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p
YGR279C3.671.20E-4SCW4Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating

GO enrichment analysis for SGTC_3113
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1102.75E-17SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.129032
0.1032.02E-15SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0634921Golgi
0.1001.74E-14SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.078125
0.0903.30E-12SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.129032
0.0881.08E-11SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.119403
0.0871.78E-11SGTC_31129123419 49.5 μMChembridge (Drug-like library)170158540.132353RPP1 & pyrimidine depletion
0.0863.02E-11SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0714286ERG2
0.0863.50E-11SGTC_32409133527 49.5 μMChembridge (Drug-like library)176174550.133333
0.0857.31E-11SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.126761
0.0807.91E-10SGTC_28489016370 26.0 μMChembridge (Drug-like library)78944100.157895
0.0791.42E-9SGTC_11710368-0077 21.4 μMChemDiv (Drug-like library)32397790.102941
0.0782.03E-9SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0853659RPP1 & pyrimidine depletion
0.0772.86E-9SGTC_2727riluzole 51.8 μMMiscellaneous50700.0434783mitochondrial processes
0.0772.98E-9SGTC_481bay k-8644 50.0 μMICCB bioactive library23030.115385
0.0773.03E-9SGTC_1964st076914 61.0 μMTimTec (Natural product derivative library)242086610.131579RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2994905352071.43 μM0.6808514647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_2835900658871.43 μM0.582345538Chembridge (Drug-like library)300.782683.27912
SGTC_3177910750649.47 μM0.5454558897194Chembridge (Drug-like library)314.809263.76512ERG2
SGTC_3178910750649.47 μM0.5454558897194Chembridge (Drug-like library)314.809263.76512
SGTC_3179910780149.47 μM0.5178574818259Chembridge (Drug-like library)318.7731433.48413
SGTC_2893904848825.97 μM0.4035096467338Chembridge (Drug-like library)337.458583.46913fatty acid desaturase (OLE1)
SGTC_2895905444758.44 μM0.3928572697450Chembridge (Drug-like library)343.850483.16113
SGTC_2838900268771.43 μM0.3548396457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_2887904478458.44 μM0.3448286465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)
SGTC_1653st01211265 μM0.338983720429TimTec (Natural product derivative library)281.348962.9202
SGTC_3208911411549.47 μM0.323529650895Chembridge (Drug-like library)357.4218633.26115