9124945

5-methyl-3-[3-(2-phenylethyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol-6-yl]-1,2-oxazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3117
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17592065
SMILES CC1=CC(=NO1)C2=NN3C(=NN=C3S2)CCC4=CC=CC=C4
Standardized SMILES Cc1onc(c1)c2nn3c(CCc4ccccc4)nnc3s2
Molecular weight 311.3616
ALogP 2.9
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.17
% growth inhibition (Hom. pool) 3.53


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17592065
Download HIP data (tab-delimited text)  (excel)
Gene:GPI18(YBR004C)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.26||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:MOB1(YIL106W)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.06||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:PFS2(YNL317W)|FD-Score:3.15|P-value:8.27E-4|Clearance:0.24||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PRP5(YBR237W)|FD-Score:3.8|P-value:7.32E-5|Clearance:0.3||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPC82(YPR190C)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C82 Gene:UTP4(YDR324C)|FD-Score:-5.62|P-value:9.46E-9|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YJL195C(YJL195C_d)|FD-Score:-4.24|P-value:1.13E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:GPI18(YBR004C)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.26||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:MOB1(YIL106W)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.06||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:PFS2(YNL317W)|FD-Score:3.15|P-value:8.27E-4|Clearance:0.24||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PRP5(YBR237W)|FD-Score:3.8|P-value:7.32E-5|Clearance:0.3||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPC82(YPR190C)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C82 Gene:UTP4(YDR324C)|FD-Score:-5.62|P-value:9.46E-9|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YJL195C(YJL195C_d)|FD-Score:-4.24|P-value:1.13E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17592065
Download HOP data (tab-delimited text)  (excel)
Gene:ANS1(YHR126C_p)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ATP7(YKL016C)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BNA2(YJR078W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:BOI2(YER114C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BTT1(YDR252W)|FD-Score:-3.11|P-value:9.35E-4||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:BUD3(YCL014W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CNB1(YKL190W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:ENT5(YDR153C)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:EOS1(YNL080C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FIS1(YIL065C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FMP10(YER182W_p)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GEP3(YOR205C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GLE2(YER107C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HNT1(YDL125C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:ICY2(YPL250C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IRC23(YOR044W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LDB7(YBL006C)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MGT1(YDL200C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:NDE1(YMR145C)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NDE2(YDL085W)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NOT3(YIL038C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PEX21(YGR239C)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PKH2(YOL100W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PKR1(YMR123W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PTC5(YOR090C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RPL14A(YKL006W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RXT3(YDL076C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SER33(YIL074C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:STV1(YMR054W)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SYH1(YPL105C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TDA8(YAL064C-A_p)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TRP3(YKL211C)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:-4.31|P-value:8.06E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YDR008C(YDR008C_d)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER091C-A(YER091C-A_d)|FD-Score:-5.48|P-value:2.12E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR050C(YGR050C_p)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR111W(YGR111W_p)|FD-Score:-4.33|P-value:7.57E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR228W(YGR228W_d)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YGR237C(YGR237C_p)|FD-Score:-5.96|P-value:1.27E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIR020C(YIR020C_p)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR124W(YLR124W_d)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR152C(YLR152C_p)|FD-Score:-3.78|P-value:7.78E-5||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:ANS1(YHR126C_p)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ATP7(YKL016C)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BNA2(YJR078W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:BOI2(YER114C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BTT1(YDR252W)|FD-Score:-3.11|P-value:9.35E-4||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:BUD3(YCL014W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CNB1(YKL190W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:ENT5(YDR153C)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:EOS1(YNL080C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FIS1(YIL065C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FMP10(YER182W_p)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GEP3(YOR205C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GLE2(YER107C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HNT1(YDL125C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:ICY2(YPL250C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IRC23(YOR044W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LDB7(YBL006C)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MGT1(YDL200C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:NDE1(YMR145C)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NDE2(YDL085W)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:NOT3(YIL038C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:PEX21(YGR239C)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PKH2(YOL100W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PKR1(YMR123W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PTC5(YOR090C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RPL14A(YKL006W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RXT3(YDL076C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SER33(YIL074C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:STV1(YMR054W)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SYH1(YPL105C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TDA8(YAL064C-A_p)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TRP3(YKL211C)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:-4.31|P-value:8.06E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YDR008C(YDR008C_d)|FD-Score:3.76|P-value:8.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER091C-A(YER091C-A_d)|FD-Score:-5.48|P-value:2.12E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR050C(YGR050C_p)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR111W(YGR111W_p)|FD-Score:-4.33|P-value:7.57E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR228W(YGR228W_d)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YGR237C(YGR237C_p)|FD-Score:-5.96|P-value:1.27E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIR020C(YIR020C_p)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR124W(YLR124W_d)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR152C(YLR152C_p)|FD-Score:-3.78|P-value:7.78E-5||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR237W3.807.32E-50.30PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YIL106W3.492.39E-40.06MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YPR190C3.442.93E-40.03RPC82RNA polymerase III subunit C82
YBR004C3.413.28E-40.26GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YNL317W3.158.27E-40.24PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YDR232W2.910.001830.02HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YNL282W2.890.001940.14POP3Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YBR136W2.740.003040.12MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YDR381W2.630.004320.04YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YBR202W2.590.004810.02MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YGR158C2.570.005090.00MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YJL061W2.570.005110.10NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YMR028W2.470.006700.00TAP42Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
YDR288W2.470.006730.01NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YNL103W2.460.007000.05MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL105C5.141.39E-7SYH1Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
YMR143W4.181.48E-5RPS16AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication
YMR123W4.092.14E-5PKR1V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
YER114C4.022.91E-5BOI2Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
YKL016C3.924.45E-5ATP7Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YMR216C3.895.04E-5SKY1SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
YDR008C_d3.768.36E-5YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDL125C3.721.00E-4HNT1Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress
YIL038C3.591.65E-4NOT3Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
YER107C3.472.57E-4GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YIL074C3.393.46E-4SER333-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication
YIL065C3.393.51E-4FIS1Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation
YLR124W_d3.393.52E-4YLR124W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YBR171W3.363.83E-4SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YOL100W3.324.57E-4PKH2Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p

GO enrichment analysis for SGTC_3117
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1503.56E-31SGTC_31069121286 49.5 μMChembridge (Drug-like library)159434710.131579
0.0912.36E-12SGTC_20325185112 26.0 μMChembridge (Fragment library)57181890.0821918
0.0912.82E-12SGTC_2234tolnaftate 200.0 μMMiscellaneous55100.109589
0.0757.19E-9SGTC_31129123419 49.5 μMChembridge (Drug-like library)170158540.0958904RPP1 & pyrimidine depletion
0.0732.09E-8SGTC_31169124943 49.5 μMChembridge (Drug-like library)419051950.333333
0.0708.05E-8SGTC_22047233102 200.0 μMChembridge (Fragment library)21724800.094594660S ribosome export
0.0663.73E-7SGTC_1885st057546 99.9 μMTimTec (Natural product derivative library)7020080.0833333
0.0663.83E-7SGTC_32319132313 49.5 μMChembridge (Drug-like library)49059670.215385
0.0663.98E-7SGTC_12310242-0792 125.0 μMChemDiv (Drug-like library)37076350.157895
0.0621.67E-6SGTC_20975376186 200.0 μMChembridge (Fragment library)7427550.15873
0.0613.55E-6SGTC_2113455-0679 143.5 μMChemDiv (Drug-like library)7486490.111111
0.0595.05E-6SGTC_32409133527 49.5 μMChembridge (Drug-like library)176174550.113924
0.0589.09E-6SGTC_2515chrysin 20.8 μMMicrosource (Natural product library)52816070.115942RPP1 & pyrimidine depletion
0.0589.46E-6SGTC_30019076041 71.4 μMChembridge (Drug-like library)160042110.128205
0.0571.33E-5SGTC_6164092-0450 108.0 μMChemDiv (Drug-like library)7871650.144928

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3210912815749.47 μM0.58490617590849Chembridge (Drug-like library)296.346983.26405
SGTC_3068911676849.47 μM0.49090941904888Chembridge (Drug-like library)286.395223.78404
SGTC_3307912688569.28 μM0.4561424824909Chembridge (Drug-like library)272.368643.77904
SGTC_3102912166949.47 μM0.41379341905072Chembridge (Drug-like library)272.368643.57504
SGTC_3073911497249.47 μM0.441905075Chembridge (Drug-like library)272.368643.5750460S ribosome export
SGTC_3101912053149.47 μM0.39682524824904Chembridge (Drug-like library)288.368043.27705
SGTC_3292912082559.78 μM0.38333324824900Chembridge (Drug-like library)276.3325233.29405
SGTC_3069911700949.47 μM0.37704941904981Chembridge (Drug-like library)284.379343.7610460S ribosome export
SGTC_2996906619271.43 μM0.3709686472854Chembridge (Drug-like library)326.3481433.41906
SGTC_2891906387619.48 μM0.3692316472478Chembridge (Drug-like library)312.346382.79206