9126162

6-(3,4-diethoxyphenyl)-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3123
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25239884
SMILES CCOC1=C(C=C(C=C1)C2=NN3C(=NN=C3S2)C4=CC=CC=N4)OCC
Standardized SMILES CCOc1ccc(cc1OCC)c2nn3c(nnc3s2)c4ccccn4
Molecular weight 367.4249
ALogP 3.32
H-bond donor count 0
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.9
% growth inhibition (Hom. pool) 7.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25239884
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.12||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:DHR2(YKL078W)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:EMW1(YNL313C)|FD-Score:-3.89|P-value:4.98E-5|Clearance:0||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:GPI12(YMR281W)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:KRE5(YOR336W)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.27||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:NOG1(YPL093W)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PCF11(YDR228C)|FD-Score:-3.72|P-value:9.78E-5|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PRT1(YOR361C)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.23||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RET3(YPL010W)|FD-Score:-4.15|P-value:1.63E-5|Clearance:0||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPC31(YNL151C)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RRP4(YHR069C)|FD-Score:3.16|P-value:7.97E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RTP1(YMR185W_p)|FD-Score:3.11|P-value:9.39E-4|Clearance:0.02||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SEC16(YPL085W)|FD-Score:-3.89|P-value:4.98E-5|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:STT4(YLR305C)|FD-Score:3.87|P-value:5.48E-5|Clearance:0.27||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:YDR526C(YDR526C_d)|FD-Score:-5.18|P-value:1.09E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR282W(YOR282W_d)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:ACS2(YLR153C)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.12||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:DHR2(YKL078W)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:EMW1(YNL313C)|FD-Score:-3.89|P-value:4.98E-5|Clearance:0||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:GPI12(YMR281W)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:KRE5(YOR336W)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.27||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:NOG1(YPL093W)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PCF11(YDR228C)|FD-Score:-3.72|P-value:9.78E-5|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PRT1(YOR361C)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.23||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RET3(YPL010W)|FD-Score:-4.15|P-value:1.63E-5|Clearance:0||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPC31(YNL151C)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RRP4(YHR069C)|FD-Score:3.16|P-value:7.97E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RTP1(YMR185W_p)|FD-Score:3.11|P-value:9.39E-4|Clearance:0.02||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SEC16(YPL085W)|FD-Score:-3.89|P-value:4.98E-5|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:STT4(YLR305C)|FD-Score:3.87|P-value:5.48E-5|Clearance:0.27||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:YDR526C(YDR526C_d)|FD-Score:-5.18|P-value:1.09E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR282W(YOR282W_d)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25239884
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ALB1(YJL122W)|FD-Score:5.34|P-value:4.59E-8||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARG5,6(YER069W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARG82(YDR173C)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG12(YBR217W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:CCM1(YGR150C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDC73(YLR418C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLA4(YNL298W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-5.22|P-value:8.85E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL4(YIR028W)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:ERG2(YMR202W)|FD-Score:-6.35|P-value:1.07E-10||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FMP10(YER182W_p)|FD-Score:6.34|P-value:1.14E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAP1(YKR039W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:HPA2(YPR193C)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:IMP2'(YIL154C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:MAC1(YMR021C)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDL2(YPL270W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MET17(YLR303W)|FD-Score:5.28|P-value:6.36E-8||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET8(YBR213W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MNN11(YJL183W)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MNN2(YBR015C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSA2(YKR077W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSY1(YPL097W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:PNS1(YOR161C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:POL32(YJR043C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRO2(YOR323C)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTR2(YKR093W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RED1(YLR263W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RPL26B(YGR034W)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-9.14|P-value:3.06E-20||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SAP155(YFR040W)|FD-Score:4.3|P-value:8.44E-6||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SCD6(YPR129W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SKN7(YHR206W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLM3(YDL033C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLX9(YGR081C)|FD-Score:4|P-value:3.18E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SLY41(YOR307C)|FD-Score:-5.47|P-value:2.27E-8||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SNU66(YOR308C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SNX3(YOR357C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SOL3(YHR163W)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TMA19(YKL056C)|FD-Score:6.16|P-value:3.60E-10||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TOM7(YNL070W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPN1(YGL186C)|FD-Score:-4.42|P-value:4.85E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP4(YDR354W)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URA4(YLR420W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VPS25(YJR102C)|FD-Score:-5.42|P-value:2.90E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS36(YLR417W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS55(YJR044C)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS63(YLR261C_d)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR072C-A(YBR072C-A_p)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR226C(YBR226C_d)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL012C(YCL012C)|FD-Score:5.3|P-value:5.72E-8||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YFL063W(YFL063W_d)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Dubious open reading frame, based on available experimental and comparative sequence data Gene:YKL033W-A(YKL033W-A_p)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YLR111W(YLR111W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR279W(YLR279W_d)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML018C(YML018C_p)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YNL010W(YNL010W_p)|FD-Score:4.45|P-value:4.21E-6||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL170W(YNL170W_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR065C(YNR065C_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR304C-A(YOR304C-A_p)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPL162C(YPL162C_p)|FD-Score:4.33|P-value:7.56E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:ABZ2(YMR289W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ALB1(YJL122W)|FD-Score:5.34|P-value:4.59E-8||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARG5,6(YER069W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARG82(YDR173C)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG12(YBR217W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:CCM1(YGR150C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDC73(YLR418C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLA4(YNL298W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-5.22|P-value:8.85E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL4(YIR028W)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:ERG2(YMR202W)|FD-Score:-6.35|P-value:1.07E-10||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FMP10(YER182W_p)|FD-Score:6.34|P-value:1.14E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAP1(YKR039W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:HPA2(YPR193C)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:IMP2'(YIL154C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:MAC1(YMR021C)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDL2(YPL270W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MET17(YLR303W)|FD-Score:5.28|P-value:6.36E-8||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET8(YBR213W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MNN11(YJL183W)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MNN2(YBR015C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSA2(YKR077W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSY1(YPL097W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:PNS1(YOR161C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:POL32(YJR043C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRO2(YOR323C)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTR2(YKR093W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RED1(YLR263W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RPL26B(YGR034W)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-9.14|P-value:3.06E-20||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SAP155(YFR040W)|FD-Score:4.3|P-value:8.44E-6||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SCD6(YPR129W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SKN7(YHR206W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLM3(YDL033C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLX9(YGR081C)|FD-Score:4|P-value:3.18E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SLY41(YOR307C)|FD-Score:-5.47|P-value:2.27E-8||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SNU66(YOR308C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SNX3(YOR357C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SOL3(YHR163W)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TMA19(YKL056C)|FD-Score:6.16|P-value:3.60E-10||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TOM7(YNL070W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPN1(YGL186C)|FD-Score:-4.42|P-value:4.85E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP4(YDR354W)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URA4(YLR420W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VPS25(YJR102C)|FD-Score:-5.42|P-value:2.90E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS36(YLR417W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS55(YJR044C)|FD-Score:-3.91|P-value:4.65E-5||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS63(YLR261C_d)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR072C-A(YBR072C-A_p)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR226C(YBR226C_d)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL012C(YCL012C)|FD-Score:5.3|P-value:5.72E-8||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YFL063W(YFL063W_d)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Dubious open reading frame, based on available experimental and comparative sequence data Gene:YKL033W-A(YKL033W-A_p)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YLR111W(YLR111W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR279W(YLR279W_d)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML018C(YML018C_p)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YNL010W(YNL010W_p)|FD-Score:4.45|P-value:4.21E-6||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL170W(YNL170W_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR065C(YNR065C_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR304C-A(YOR304C-A_p)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPL162C(YPL162C_p)|FD-Score:4.33|P-value:7.56E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR305C3.875.48E-50.27STT4Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
YOR336W3.787.96E-50.27KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YOR361C3.512.26E-40.23PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YLR153C3.285.25E-40.12ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YHR069C3.167.97E-40.05RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YMR185W_p3.119.39E-40.02RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YCR052W3.090.001010.09RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YPR107C3.000.001368.50E-4YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YER168C3.000.001370.03CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YIL061C2.970.001510.04SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YIR015W2.920.001750.11RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YBR011C2.810.002470.01IPP1Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
YLR005W2.800.002570.01SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YER094C2.790.002650.04PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YKL104C2.750.002960.15GFA1Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR163W7.331.18E-13SOL36-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
YER182W_p6.341.14E-10FMP10_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YKL056C6.163.60E-10TMA19Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress
YKL033W-A_p5.591.16E-8YKL033W-A_pPutative protein of unknown function; similar to uncharacterized proteins from other fungi
YJL122W5.344.59E-8ALB1Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
YCL012C5.305.72E-8YCL012CPutative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
YLR303W5.286.36E-8MET17Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis
YLR263W4.751.02E-6RED1Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
YNR006W4.681.45E-6VPS27Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
YNL010W_p4.454.21E-6YNL010W_pPutative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation
YGR150C4.424.90E-6CCM1Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport
YDR173C4.415.11E-6ARG82Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
YPL162C_p4.337.56E-6YPL162C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
YFR040W4.308.44E-6SAP155Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress
YOR308C4.092.20E-5SNU66Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1

GO enrichment analysis for SGTC_3123
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0993.21E-14SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.025974TSC3-RPN4
0.0937.22E-13SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.091954
0.0903.45E-12SGTC_31349127454 49.5 μMChembridge (Drug-like library)242628200.0933333
0.0871.76E-11SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.093023360S ribosome export
0.0872.10E-11SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.0632911
0.0862.92E-11SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0694444TSC3-RPN4
0.0849.14E-11SGTC_31219125696 49.5 μMChembridge (Drug-like library)12353650.333333
0.0831.85E-10SGTC_720r052-1644 49.9 μMChemDiv (Drug-like library)14228630.060241TSC3-RPN4
0.0831.99E-10SGTC_31359127742 49.5 μMChembridge (Drug-like library)36434680.0909091
0.0814.43E-10SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.141176
0.0809.48E-10SGTC_12320279-0259 161.0 μMChemDiv (Drug-like library)15600000.0813954
0.0773.03E-9SGTC_290299-0004 13.0 μMChemDiv (Drug-like library)168710.107692
0.0773.19E-9SGTC_1773st048542 73.6 μMTimTec (Natural product derivative library)32371380.0909091
0.0773.26E-9SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0609756TSC3-RPN4
0.0773.90E-9SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3107912180849.47 μM0.631579662681Chembridge (Drug-like library)309.345742.6406
SGTC_3293912093962.05 μM0.578947934087Chembridge (Drug-like library)309.345742.6406
SGTC_3099912036749.47 μM0.52631624824940Chembridge (Drug-like library)405.216293.23405
SGTC_3100912053649.47 μM0.50819716421196Chembridge (Drug-like library)319.340563.25106
SGTC_3124912756749.47 μM0.4761917592030Chembridge (Drug-like library)323.372322.67406DNA damage response
SGTC_3122912630949.47 μM0.437517591206Chembridge (Drug-like library)343.405023.59905cell wall signaling
SGTC_3132912670649.47 μM0.38806850858Chembridge (Drug-like library)326.3481433.56706
SGTC_3016908086649.47 μM0.37517066299Chembridge (Drug-like library)331.392781.94116
SGTC_3101912053149.47 μM0.37313424824904Chembridge (Drug-like library)288.368043.27705
SGTC_3083911746549.47 μM0.36111116420915Chembridge (Drug-like library)378.235863.39206