9127691

(3-aminothieno[2,3-b]pyridin-2-yl)-(4-phenylpiperazin-1-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3128
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 41316314
SMILES C1CN(CCN1C2=CC=CC=C2)C(=O)C3=C(C4=C(S3)N=CC=C4)N
Standardized SMILES Nc1c(sc2ncccc12)C(=O)N3CCN(CC3)c4ccccc4
Molecular weight 338.4267
ALogP 2.33
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.56
% growth inhibition (Hom. pool) 6.66


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 41316314
Download HIP data (tab-delimited text)  (excel)
Gene:CDC28(YBR160W)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.07||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:MED7(YOL135C)|FD-Score:3.1|P-value:9.64E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOP56(YLR197W)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.16||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PRE8(YML092C)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.01||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RRP46(YGR095C)|FD-Score:3.7|P-value:1.10E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RTT105(YER104W)|FD-Score:3.28|P-value:5.11E-4|Clearance:0.09||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC12(YNR026C)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.04||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:TID3(YIL144W)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UFE1(YOR075W)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.08||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:UTP6(YDR449C)|FD-Score:4.73|P-value:1.11E-6|Clearance:1.04||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YLR140W(YLR140W_d)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPT1(YFL038C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.08||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:YRB1(YDR002W)|FD-Score:-3.23|P-value:6.23E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:CDC28(YBR160W)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.07||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:MED7(YOL135C)|FD-Score:3.1|P-value:9.64E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOP56(YLR197W)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.16||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PRE8(YML092C)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.01||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RRP46(YGR095C)|FD-Score:3.7|P-value:1.10E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RTT105(YER104W)|FD-Score:3.28|P-value:5.11E-4|Clearance:0.09||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC12(YNR026C)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.04||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:TID3(YIL144W)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UFE1(YOR075W)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.08||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:UTP6(YDR449C)|FD-Score:4.73|P-value:1.11E-6|Clearance:1.04||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YLR140W(YLR140W_d)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPT1(YFL038C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.08||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:YRB1(YDR002W)|FD-Score:-3.23|P-value:6.23E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 41316314
Download HOP data (tab-delimited text)  (excel)
Gene:ALY1(YKR021W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:APT1(YML022W)|FD-Score:-4.47|P-value:3.97E-6||SGD DESC:Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG32(YIL146C)|FD-Score:-4.93|P-value:4.18E-7||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATO3(YDR384C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:AVT4(YNL101W)|FD-Score:-4.62|P-value:1.94E-6||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCK2(YER167W)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:DAL2(YIR029W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCC1(YCL016C)|FD-Score:5.68|P-value:6.92E-9||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DUS4(YLR405W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:EAF5(YEL018W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:END3(YNL084C)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERV14(YGL054C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FCY1(YPR062W)|FD-Score:5.07|P-value:1.95E-7||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:HCH1(YNL281W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HEM14(YER014W)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HMO1(YDR174W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IDP1(YDL066W)|FD-Score:4.91|P-value:4.58E-7||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:ILM1(YJR118C)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INO4(YOL108C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IRS4(YKR019C)|FD-Score:7.33|P-value:1.11E-13||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LAT1(YNL071W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:MAP2(YBL091C)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MPA43(YNL249C)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRP4(YHL004W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MZM1(YDR493W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NIP100(YPL174C)|FD-Score:6.69|P-value:1.11E-11||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:PDR12(YPL058C)|FD-Score:4.31|P-value:8.12E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PIL1(YGR086C)|FD-Score:-3.82|P-value:6.68E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:POL4(YCR014C)|FD-Score:4.3|P-value:8.67E-6||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:PPA2(YMR267W)|FD-Score:4.94|P-value:3.99E-7||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PTR2(YKR093W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RRD1(YIL153W)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SLK19(YOR195W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SNF7(YLR025W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SSN3(YPL042C)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STE20(YHL007C)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:TPN1(YGL186C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRK1(YJL129C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:URE2(YNL229C)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:UTR2(YEL040W)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck Gene:VOA1(YGR106C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS28(YPL065W)|FD-Score:5.05|P-value:2.21E-7||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS34(YLR240W)|FD-Score:-7.57|P-value:1.87E-14||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YBR027C(YBR027C_d)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL124W(YDL124W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR090C(YDR090C_p)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Putative protein of unknown function Gene:YDR115W(YDR115W_p)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR433W(YDR433W_d)|FD-Score:5.03|P-value:2.49E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR015C(YGR015C_p)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YGR079W(YGR079W_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YIL001W(YIL001W_p)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YKL033W-A(YKL033W-A_p)|FD-Score:4.79|P-value:8.20E-7||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKR011C(YKR011C_p)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR118C(YLR118C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR171W(YLR171W_d)|FD-Score:4.76|P-value:9.77E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR217W(YLR217W_d)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YMR262W(YMR262W_p)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YMR326C(YMR326C_d)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:YOL099C(YOL099C_d)|FD-Score:4.44|P-value:4.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPL162C(YPL162C_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL229W(YPL229W_p)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:ALY1(YKR021W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:APT1(YML022W)|FD-Score:-4.47|P-value:3.97E-6||SGD DESC:Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG32(YIL146C)|FD-Score:-4.93|P-value:4.18E-7||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATO3(YDR384C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:AVT4(YNL101W)|FD-Score:-4.62|P-value:1.94E-6||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCK2(YER167W)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:DAL2(YIR029W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCC1(YCL016C)|FD-Score:5.68|P-value:6.92E-9||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DUS4(YLR405W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:EAF5(YEL018W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:END3(YNL084C)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERV14(YGL054C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FCY1(YPR062W)|FD-Score:5.07|P-value:1.95E-7||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:HCH1(YNL281W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HEM14(YER014W)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HMO1(YDR174W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IDP1(YDL066W)|FD-Score:4.91|P-value:4.58E-7||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:ILM1(YJR118C)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INO4(YOL108C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IRS4(YKR019C)|FD-Score:7.33|P-value:1.11E-13||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST1(YNL265C)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LAT1(YNL071W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:MAP2(YBL091C)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MPA43(YNL249C)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRP4(YHL004W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MZM1(YDR493W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NIP100(YPL174C)|FD-Score:6.69|P-value:1.11E-11||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:PDR12(YPL058C)|FD-Score:4.31|P-value:8.12E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PIL1(YGR086C)|FD-Score:-3.82|P-value:6.68E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:POL4(YCR014C)|FD-Score:4.3|P-value:8.67E-6||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:PPA2(YMR267W)|FD-Score:4.94|P-value:3.99E-7||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PTR2(YKR093W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RRD1(YIL153W)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SLK19(YOR195W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SNF7(YLR025W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SSN3(YPL042C)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STE20(YHL007C)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:TPN1(YGL186C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRK1(YJL129C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:URE2(YNL229C)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:UTR2(YEL040W)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck Gene:VOA1(YGR106C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS28(YPL065W)|FD-Score:5.05|P-value:2.21E-7||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS34(YLR240W)|FD-Score:-7.57|P-value:1.87E-14||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YBR027C(YBR027C_d)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL124W(YDL124W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR090C(YDR090C_p)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Putative protein of unknown function Gene:YDR115W(YDR115W_p)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR433W(YDR433W_d)|FD-Score:5.03|P-value:2.49E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR015C(YGR015C_p)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YGR079W(YGR079W_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YIL001W(YIL001W_p)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YKL033W-A(YKL033W-A_p)|FD-Score:4.79|P-value:8.20E-7||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKR011C(YKR011C_p)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR118C(YLR118C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR171W(YLR171W_d)|FD-Score:4.76|P-value:9.77E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR217W(YLR217W_d)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YMR262W(YMR262W_p)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YMR326C(YMR326C_d)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:YOL099C(YOL099C_d)|FD-Score:4.44|P-value:4.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPL162C(YPL162C_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL229W(YPL229W_p)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR449C4.731.11E-61.04UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGR095C3.701.10E-40.05RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YFL038C3.641.35E-40.08YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YLR197W3.561.85E-40.16NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YNR026C3.403.32E-40.04SEC12Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
YLR140W_d3.373.81E-40.01YLR140W_dDubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I
YBR160W3.354.00E-40.07CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YER104W3.285.11E-40.09RTT105Protein with a role in regulation of Ty1 transposition
YOR075W3.197.12E-40.08UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YML092C3.119.43E-40.01PRE8Alpha 2 subunit of the 20S proteasome
YOL135C3.109.64E-40.00MED7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YMR290W-A_d3.109.73E-40.13YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YJL091C2.970.001500.24GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YJR112W2.720.003220.07NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YMR240C2.660.003940.00CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR019C7.331.11E-13IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YPL174C6.691.11E-11NIP100Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
YCL016C5.686.92E-9DCC1Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance
YPR062W5.071.95E-7FCY1Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
YPL065W5.052.21E-7VPS28Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
YDR433W_d5.032.49E-7YDR433W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR267W4.943.99E-7PPA2Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate
YDL066W4.914.58E-7IDP1Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
YKL033W-A_p4.798.20E-7YKL033W-A_pPutative protein of unknown function; similar to uncharacterized proteins from other fungi
YLR171W_d4.769.77E-7YLR171W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL084C4.651.64E-6END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YNL265C4.542.83E-6IST1Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p
YIL153W4.464.10E-6RRD1Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YPL042C4.454.31E-6SSN3Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
YOL099C_d4.444.53E-6YOL099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes

GO enrichment analysis for SGTC_3128
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1163.18E-19SGTC_24605552655 174.2 μMMiscellaneous54019890.179104amide catabolism
0.1024.88E-15SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.177419Golgi
0.0897.76E-12SGTC_14103909-8106 77.9 μMChemDiv (Drug-like library)10392100.0930233
0.0822.93E-10SGTC_1669st015249 58.4 μMTimTec (Natural product derivative library)7227930.0921053Golgi
0.0807.05E-10SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.0588235Golgi
0.0791.12E-9SGTC_2413inermin 26.1 μMTimTec (Natural product derivative library)915100.0533333
0.0791.26E-9SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.0810811
0.0782.34E-9SGTC_31749107204 49.5 μMChembridge (Drug-like library)39388020.125ERG2
0.0758.85E-9SGTC_1972st070201 17.4 μMTimTec (Natural product derivative library)28290990.144737
0.0758.92E-9SGTC_9731325-0077 65.9 μMChemDiv (Drug-like library)28351490.0882353
0.0714.38E-8SGTC_1751st044839 27.2 μMTimTec (Natural product derivative library)52913260.0853659Golgi
0.0707.14E-8SGTC_30999120367 49.5 μMChembridge (Drug-like library)248249400.15493
0.0708.54E-8SGTC_21205538279 152.0 μMChembridge (Fragment library)20581970.140625
0.0672.68E-7SGTC_389tpck 16.7 μMMiscellaneous4396470.123288
0.0664.87E-7SGTC_30349090421 49.5 μMChembridge (Drug-like library)171737610.15789560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3096911746849.47 μM0.7391341316311Chembridge (Drug-like library)247.316121.1614
SGTC_22267298446200 μM0.406783283010Chembridge (Fragment library)287.379982.70104
SGTC_2147554593289.72 μM0.392157797404Chembridge (Fragment library)232.32142.33802ergosterol biosynthesis
SGTC_20204017018200 μM0.38775521127491Chembridge (Fragment library)320.36530.23957
SGTC_21645685496200 μM0.355932759046Chembridge (Fragment library)257.28781.65704
SGTC_22867945697125 μM0.3548391245733Chembridge (Fragment library)287.379982.06904
SGTC_22887946289167.31 μM0.3442621246052Chembridge (Fragment library)271.314381.80204
SGTC_1779st03942755 μM0.3432841124498TimTec (Natural product derivative library)363.45283.50103
SGTC_1832st05411355 μM0.3333331251267TimTec (Natural product derivative library)364.39783.37116
SGTC_3143908665849.47 μM0.31666725236626Chembridge (Drug-like library)300.41853.45803