9126706

3-(3-fluorophenyl)-6-(2-methoxyphenyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3132
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 850858
SMILES COC1=CC=CC=C1C2=NN3C(=NN=C3S2)C4=CC(=CC=C4)F
Standardized SMILES COc1ccccc1c2nn3c(nnc3s2)c4cccc(F)c4
Molecular weight 326.3481
ALogP 3.57
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.05
% growth inhibition (Hom. pool) -2.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 850858
Download HIP data (tab-delimited text)  (excel)
Gene:ATP16(YDL004W)|FD-Score:-3.48|P-value:2.49E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BET1(YIL004C)|FD-Score:4.61|P-value:2.00E-6|Clearance:0.25||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:BRN1(YBL097W)|FD-Score:4.36|P-value:6.53E-6|Clearance:0.23||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DUT1(YBR252W)|FD-Score:-3.91|P-value:4.66E-5|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:EPL1(YFL024C)|FD-Score:3.13|P-value:8.83E-4|Clearance:0.08||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:GPI10(YGL142C)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.14||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:GPI11(YDR302W)|FD-Score:4.66|P-value:1.61E-6|Clearance:0.05||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:NOG1(YPL093W)|FD-Score:-8.06|P-value:3.87E-16|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NPA3(YJR072C)|FD-Score:4.08|P-value:2.22E-5|Clearance:0.6||SGD DESC:Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress Gene:PRI1(YIR008C)|FD-Score:4.85|P-value:6.24E-7|Clearance:0.19||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RIO1(YOR119C)|FD-Score:-4.84|P-value:6.45E-7|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPL15A(YLR029C)|FD-Score:6.3|P-value:1.46E-10|Clearance:0.68||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPS3(YNL178W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.06||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP4(YHR069C)|FD-Score:-4.6|P-value:2.09E-6|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC9(YML127W)|FD-Score:4.13|P-value:1.80E-5|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC12(YNR026C)|FD-Score:5.04|P-value:2.32E-7|Clearance:0.19||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SRP101(YDR292C)|FD-Score:5.62|P-value:9.41E-9|Clearance:0.58||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:SSY1(YDR160W)|FD-Score:-3.47|P-value:2.59E-4|Clearance:0||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TEN1(YLR010C)|FD-Score:-3.29|P-value:5.04E-4|Clearance:0||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TFG2(YGR005C)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.11||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TIF35(YDR429C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YKL153W(YKL153W_d)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:ATP16(YDL004W)|FD-Score:-3.48|P-value:2.49E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BET1(YIL004C)|FD-Score:4.61|P-value:2.00E-6|Clearance:0.25||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:BRN1(YBL097W)|FD-Score:4.36|P-value:6.53E-6|Clearance:0.23||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DUT1(YBR252W)|FD-Score:-3.91|P-value:4.66E-5|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:EPL1(YFL024C)|FD-Score:3.13|P-value:8.83E-4|Clearance:0.08||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:GPI10(YGL142C)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.14||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:GPI11(YDR302W)|FD-Score:4.66|P-value:1.61E-6|Clearance:0.05||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:NOG1(YPL093W)|FD-Score:-8.06|P-value:3.87E-16|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NPA3(YJR072C)|FD-Score:4.08|P-value:2.22E-5|Clearance:0.6||SGD DESC:Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress Gene:PRI1(YIR008C)|FD-Score:4.85|P-value:6.24E-7|Clearance:0.19||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RIO1(YOR119C)|FD-Score:-4.84|P-value:6.45E-7|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPL15A(YLR029C)|FD-Score:6.3|P-value:1.46E-10|Clearance:0.68||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPS3(YNL178W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.06||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP4(YHR069C)|FD-Score:-4.6|P-value:2.09E-6|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC9(YML127W)|FD-Score:4.13|P-value:1.80E-5|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC12(YNR026C)|FD-Score:5.04|P-value:2.32E-7|Clearance:0.19||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SRP101(YDR292C)|FD-Score:5.62|P-value:9.41E-9|Clearance:0.58||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:SSY1(YDR160W)|FD-Score:-3.47|P-value:2.59E-4|Clearance:0||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TEN1(YLR010C)|FD-Score:-3.29|P-value:5.04E-4|Clearance:0||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TFG2(YGR005C)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.11||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TIF35(YDR429C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YKL153W(YKL153W_d)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 850858
Download HOP data (tab-delimited text)  (excel)
Gene:AFI1(YOR129C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AIM20(YIL158W)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:APQ13(YJL075C_d)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARO1(YDR127W)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG21(YPL100W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:AXL1(YPR122W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BEM2(YER155C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BNA3(YJL060W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:COA1(YIL157C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COX19(YLL018C-A)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DOT6(YER088C)|FD-Score:3.74|P-value:9.17E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DTD1(YDL219W)|FD-Score:-5.17|P-value:1.18E-7||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:ECM29(YHL030W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ECM7(YLR443W)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EMC5(YIL027C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ENV10(YLR065C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERG2(YMR202W)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FLC2(YAL053W)|FD-Score:-6.93|P-value:2.18E-12||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FPR1(YNL135C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FRK1(YPL141C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FYV1(YDR024W_d)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GDA1(YEL042W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GLO4(YOR040W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GMH1(YKR030W)|FD-Score:10.9|P-value:3.38E-28||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GSY2(YLR258W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HSM3(YBR272C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:INO4(YOL108C)|FD-Score:6.46|P-value:5.09E-11||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:ITC1(YGL133W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KAP120(YPL125W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LAG2(YOL025W)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MGA2(YIR033W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MIG1(YGL035C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MNN10(YDR245W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MRPS35(YGR165W)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NDJ1(YOL104C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:OAR1(YKL055C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OCH1(YGL038C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:OSH6(YKR003W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PET309(YLR067C)|FD-Score:-3.73|P-value:9.73E-5||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PFK2(YMR205C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO81(YGR233C)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PMT2(YAL023C)|FD-Score:4.78|P-value:8.76E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PTR2(YKR093W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAD18(YCR066W)|FD-Score:5.98|P-value:1.12E-9||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:REC104(YHR157W)|FD-Score:4.48|P-value:3.70E-6||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RPL17B(YJL177W)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:-4.52|P-value:3.13E-6||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RVS161(YCR009C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC1(YKL212W)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SGE1(YPR198W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKG1(YKR100C)|FD-Score:-3.89|P-value:4.98E-5||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SKP2(YNL311C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLT2(YHR030C)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF4(YGL115W)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SUR2(YDR297W)|FD-Score:-3.77|P-value:8.30E-5||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TRP4(YDR354W)|FD-Score:4.86|P-value:5.82E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VBA3(YCL069W)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VHS2(YIL135C)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VPS24(YKL041W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YBL029C-A(YBL029C-A_p)|FD-Score:-4.3|P-value:8.48E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL039W-B(YBL039W-B_p)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Putative protein of unknown function Gene:YBR285W(YBR285W_p)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCR087W(YCR087W_d)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR442W(YDR442W_d)|FD-Score:5.97|P-value:1.21E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER137C(YER137C_p)|FD-Score:4.32|P-value:7.63E-6||SGD DESC:Putative protein of unknown function Gene:YGK3(YOL128C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YHL005C(YHL005C_d)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YHR210C(YHR210C_p)|FD-Score:4.77|P-value:9.31E-7||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL055C(YIL055C_p)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative protein of unknown function Gene:YKL033W-A(YKL033W-A_p)|FD-Score:5.28|P-value:6.56E-8||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKL096C-B(YKL096C-B_p)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR177W(YLR177W_p)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Putative protein of unknown function Gene:YMR082C(YMR082C_d)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL170W(YNL170W_d)|FD-Score:6.44|P-value:5.88E-11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR199W(YOR199W_d)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR345C(YOR345C_d)|FD-Score:-4.01|P-value:2.98E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPL102C(YPL102C_d)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:AFI1(YOR129C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AIM20(YIL158W)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:APQ13(YJL075C_d)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARO1(YDR127W)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG21(YPL100W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:AXL1(YPR122W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BEM2(YER155C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BNA3(YJL060W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:COA1(YIL157C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COX19(YLL018C-A)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DOT6(YER088C)|FD-Score:3.74|P-value:9.17E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DTD1(YDL219W)|FD-Score:-5.17|P-value:1.18E-7||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:ECM29(YHL030W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ECM7(YLR443W)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EMC5(YIL027C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ENV10(YLR065C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERG2(YMR202W)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FLC2(YAL053W)|FD-Score:-6.93|P-value:2.18E-12||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FPR1(YNL135C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FRK1(YPL141C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FYV1(YDR024W_d)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GDA1(YEL042W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GLO4(YOR040W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GMH1(YKR030W)|FD-Score:10.9|P-value:3.38E-28||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GSY2(YLR258W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HSM3(YBR272C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:INO4(YOL108C)|FD-Score:6.46|P-value:5.09E-11||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:ITC1(YGL133W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KAP120(YPL125W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:LAG2(YOL025W)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MGA2(YIR033W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MIG1(YGL035C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MNN10(YDR245W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MRPS35(YGR165W)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NDJ1(YOL104C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:OAR1(YKL055C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OCH1(YGL038C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:OSH6(YKR003W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PET309(YLR067C)|FD-Score:-3.73|P-value:9.73E-5||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PFK2(YMR205C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO81(YGR233C)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PMT2(YAL023C)|FD-Score:4.78|P-value:8.76E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PTR2(YKR093W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAD18(YCR066W)|FD-Score:5.98|P-value:1.12E-9||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:REC104(YHR157W)|FD-Score:4.48|P-value:3.70E-6||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RPL17B(YJL177W)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:-4.52|P-value:3.13E-6||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RVS161(YCR009C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC1(YKL212W)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SGE1(YPR198W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKG1(YKR100C)|FD-Score:-3.89|P-value:4.98E-5||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SKP2(YNL311C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLT2(YHR030C)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF4(YGL115W)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SUR2(YDR297W)|FD-Score:-3.77|P-value:8.30E-5||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TRP4(YDR354W)|FD-Score:4.86|P-value:5.82E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VBA3(YCL069W)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VHS2(YIL135C)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VPS24(YKL041W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YBL029C-A(YBL029C-A_p)|FD-Score:-4.3|P-value:8.48E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL039W-B(YBL039W-B_p)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Putative protein of unknown function Gene:YBR285W(YBR285W_p)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCR087W(YCR087W_d)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR442W(YDR442W_d)|FD-Score:5.97|P-value:1.21E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER137C(YER137C_p)|FD-Score:4.32|P-value:7.63E-6||SGD DESC:Putative protein of unknown function Gene:YGK3(YOL128C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YHL005C(YHL005C_d)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YHR210C(YHR210C_p)|FD-Score:4.77|P-value:9.31E-7||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL055C(YIL055C_p)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative protein of unknown function Gene:YKL033W-A(YKL033W-A_p)|FD-Score:5.28|P-value:6.56E-8||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKL096C-B(YKL096C-B_p)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR177W(YLR177W_p)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Putative protein of unknown function Gene:YMR082C(YMR082C_d)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL170W(YNL170W_d)|FD-Score:6.44|P-value:5.88E-11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR199W(YOR199W_d)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR345C(YOR345C_d)|FD-Score:-4.01|P-value:2.98E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPL102C(YPL102C_d)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR029C6.301.46E-100.68RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YDR292C5.629.41E-90.58SRP101Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
YNR026C5.042.32E-70.19SEC12Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
YIR008C4.856.24E-70.19PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YDR302W4.661.61E-60.05GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YIL004C4.612.00E-60.25BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YBL097W4.366.53E-60.23BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YML127W4.131.80E-50.05RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YJR072C4.082.22E-50.60NPA3Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
YGL142C3.482.49E-40.14GPI10Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
YGR005C3.344.17E-40.11TFG2TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
YKL153W_d3.246.07E-40.05YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YNL178W3.197.13E-40.06RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YFL024C3.138.83E-40.08EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YJL054W3.050.001160.01TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR030W10.903.38E-28GMH1Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting
YOL108C6.465.09E-11INO4Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain
YNL170W_d6.445.88E-11YNL170W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR066W5.981.12E-9RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YDR442W_d5.971.21E-9YDR442W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL033W-A_p5.286.56E-8YKL033W-A_pPutative protein of unknown function; similar to uncharacterized proteins from other fungi
YDR354W4.865.82E-7TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YAL023C4.788.76E-7PMT2Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication
YHR210C_p4.779.31E-7YHR210C_pPutative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions
YHR157W4.483.70E-6REC104Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
YOR199W_d4.483.78E-6YOR199W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER137C_p4.327.63E-6YER137C_pPutative protein of unknown function
YDR127W4.289.51E-6ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YKL096C-B_p4.231.14E-5YKL096C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YGL038C4.221.22E-5OCH1Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins

GO enrichment analysis for SGTC_3132
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1086.22E-17SGTC_1104tacrolimus 29.8 μMNIH Clinical Collection235817960.0381679
0.0977.30E-14SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.030303
0.0805.99E-10SGTC_20245144227 134.0 μMChembridge (Fragment library)34752110.109589
0.0756.45E-9SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.0823529
0.0759.55E-9SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0208333
0.0731.67E-8SGTC_12131315-0166 115.0 μMChemDiv (Drug-like library)7684010.131579
0.0723.63E-8SGTC_13461488-2224 62.4 μMChemDiv (Drug-like library)37224270.123288
0.0714.59E-8SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0555556
0.0699.44E-8SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0609756
0.0691.09E-7SGTC_24865283202 22.4 μMMiscellaneous13777510.0714286
0.0691.20E-7SGTC_23017769476 200.0 μMChembridge (Fragment library)9778200.0921053
0.0682.08E-7SGTC_1833914-0051 30.6 μMChemDiv (Drug-like library)15962320.11111160S ribosome export
0.0672.81E-7SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.115385
0.0656.03E-7SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.060241TSC3-RPN4
0.0649.30E-7SGTC_24725377652 49.4 μMMiscellaneous16109030.0810811TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3293912093962.05 μM0.611111934087Chembridge (Drug-like library)309.345742.6406
SGTC_3121912569649.47 μM0.5344831235365Chembridge (Drug-like library)326.3481433.41906
SGTC_3107912180849.47 μM0.47541662681Chembridge (Drug-like library)309.345742.6406
SGTC_3099912036749.47 μM0.424824940Chembridge (Drug-like library)405.216293.23405
SGTC_3292912082559.78 μM0.424824900Chembridge (Drug-like library)276.3325233.29405
SGTC_3101912053149.47 μM0.39062524824904Chembridge (Drug-like library)288.368043.27705
SGTC_3123912616249.47 μM0.3880625239884Chembridge (Drug-like library)367.424883.32107
SGTC_2797785909881.82 μM0.387097972681Chembridge (Drug-like library)342.802743.87805
SGTC_2996906619271.43 μM0.3870976472854Chembridge (Drug-like library)326.3481433.41906
SGTC_3102912166949.47 μM0.36065641905072Chembridge (Drug-like library)272.368643.57504