9127736

4-fluoro-N-(3-hydroxyphenyl)-3-(4-methylpiperidin-1-yl)sulfonylbenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3133
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 27243420
SMILES CC1CCN(CC1)S(=O)(=O)C2=C(C=CC(=C2)C(=O)NC3=CC(=CC=C3)O)F
Standardized SMILES CC1CCN(CC1)S(=O)(=O)c2cc(ccc2F)C(=O)Nc3cccc(O)c3
Molecular weight 392.4444
ALogP 2.86
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -1.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 27243420
Download HIP data (tab-delimited text)  (excel)
Gene:CWC2(YDL209C)|FD-Score:-4.43|P-value:4.73E-6|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:NBP1(YLR457C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NNF1(YJR112W)|FD-Score:-3.25|P-value:5.69E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:PDS5(YMR076C)|FD-Score:-3.34|P-value:4.14E-4|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRE1(YER012W)|FD-Score:5.37|P-value:4.01E-8|Clearance:1.56||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RFC2(YJR068W)|FD-Score:-3.81|P-value:7.00E-5|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPS3(YNL178W)|FD-Score:3.81|P-value:6.99E-5|Clearance:0.38||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:TAF12(YDR145W)|FD-Score:-4.13|P-value:1.82E-5|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF9(YMR236W)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TAP42(YMR028W)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.45||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:VRG4(YGL225W)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YAH1(YPL252C)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YTM1(YOR272W)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:CWC2(YDL209C)|FD-Score:-4.43|P-value:4.73E-6|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:NBP1(YLR457C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NNF1(YJR112W)|FD-Score:-3.25|P-value:5.69E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:PDS5(YMR076C)|FD-Score:-3.34|P-value:4.14E-4|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRE1(YER012W)|FD-Score:5.37|P-value:4.01E-8|Clearance:1.56||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RFC2(YJR068W)|FD-Score:-3.81|P-value:7.00E-5|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPS3(YNL178W)|FD-Score:3.81|P-value:6.99E-5|Clearance:0.38||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:TAF12(YDR145W)|FD-Score:-4.13|P-value:1.82E-5|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF9(YMR236W)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TAP42(YMR028W)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.45||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:VRG4(YGL225W)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YAH1(YPL252C)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YTM1(YOR272W)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 27243420
Download HOP data (tab-delimited text)  (excel)
Gene:AIM3(YBR108W)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALE1(YOR175C)|FD-Score:5.32|P-value:5.19E-8||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ALG8(YOR067C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ASG1(YIL130W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATG12(YBR217W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:ATG16(YMR159C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BCH2(YKR027W)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BIK1(YCL029C)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BUD25(YER014C-A)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:COG1(YGL223C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX19(YLL018C-A)|FD-Score:-4.69|P-value:1.39E-6||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DEP1(YAL013W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DSS4(YPR017C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EMC4(YGL231C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:END3(YNL084C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERP5(YHR110W)|FD-Score:5.17|P-value:1.19E-7||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCN4(YEL009C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCV3(YAL044C)|FD-Score:4.95|P-value:3.72E-7||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GSP2(YOR185C)|FD-Score:-3.98|P-value:3.50E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HOM3(YER052C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSP104(YLL026W)|FD-Score:4.55|P-value:2.62E-6||SGD DESC:Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:IGO1(YNL157W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p Gene:IME4(YGL192W)|FD-Score:-5.07|P-value:1.97E-7||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:LAG2(YOL025W)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LCB5(YLR260W)|FD-Score:-5.3|P-value:5.72E-8||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MIT1(YEL007W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 Gene:MRP7(YNL005C)|FD-Score:-4.08|P-value:2.20E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MUP3(YHL036W)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NOT3(YIL038C)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NYV1(YLR093C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:ORM1(YGR038W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PEX5(YDR244W)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PHO80(YOL001W)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:POP2(YNR052C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PSH1(YOL054W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PTH1(YHR189W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RHR2(YIL053W)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL19B(YBL027W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT102(YGR275W)|FD-Score:-3.81|P-value:7.08E-5||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SEC72(YLR292C)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGS1(YMR190C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SKY1(YMR216C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLX9(YGR081C)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SLZ1(YNL196C_p)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SMP1(YBR182C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication Gene:SMY1(YKL079W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SOP4(YJL192C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRB8(YCR081W)|FD-Score:4.96|P-value:3.50E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRN2(YLR119W)|FD-Score:4.58|P-value:2.34E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SUS1(YBR111W-A)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWI3(YJL176C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TDA6(YPR157W_p)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:THI6(YPL214C)|FD-Score:3.79|P-value:7.47E-5||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TIF4631(YGR162W)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TMA19(YKL056C)|FD-Score:6.07|P-value:6.23E-10||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TPM1(YNL079C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:VAM6(YDL077C)|FD-Score:-4.32|P-value:7.87E-6||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA11(YPL234C)|FD-Score:-4.41|P-value:5.27E-6||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS28(YPL065W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS41(YDR080W)|FD-Score:-3.79|P-value:7.66E-5||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS61(YDR136C_d)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS9(YML097C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBT1(YLL048C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR417C(YDR417C_d)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YDR444W(YDR444W_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGL176C(YGL176C_p)|FD-Score:-3.78|P-value:7.94E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR122C-A(YGR122C-A_d)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YIL152W(YIL152W_p)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Putative protein of unknown function Gene:YLL007C(YLL007C_p)|FD-Score:-3.92|P-value:4.50E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YML047W-A(YML047W-A_d)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR119W-A(YMR119W-A_d)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR320W(YMR320W_d)|FD-Score:4.82|P-value:7.04E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL035C(YNL035C_p)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPL113C(YPL113C_p)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPL150W(YPL150W_p)|FD-Score:5.07|P-value:1.98E-7||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:AIM3(YBR108W)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALE1(YOR175C)|FD-Score:5.32|P-value:5.19E-8||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ALG8(YOR067C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ASG1(YIL130W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATG12(YBR217W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:ATG16(YMR159C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BCH2(YKR027W)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BIK1(YCL029C)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BUD25(YER014C-A)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:COG1(YGL223C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX19(YLL018C-A)|FD-Score:-4.69|P-value:1.39E-6||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DEP1(YAL013W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DSS4(YPR017C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EMC4(YGL231C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:END3(YNL084C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERP5(YHR110W)|FD-Score:5.17|P-value:1.19E-7||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCN4(YEL009C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCV3(YAL044C)|FD-Score:4.95|P-value:3.72E-7||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GSP2(YOR185C)|FD-Score:-3.98|P-value:3.50E-5||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HOM3(YER052C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSP104(YLL026W)|FD-Score:4.55|P-value:2.62E-6||SGD DESC:Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress Gene:IGO1(YNL157W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p Gene:IME4(YGL192W)|FD-Score:-5.07|P-value:1.97E-7||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:LAG2(YOL025W)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LCB5(YLR260W)|FD-Score:-5.3|P-value:5.72E-8||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MIT1(YEL007W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 Gene:MRP7(YNL005C)|FD-Score:-4.08|P-value:2.20E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MUP3(YHL036W)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NOT3(YIL038C)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NYV1(YLR093C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:ORM1(YGR038W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PEX5(YDR244W)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PHO80(YOL001W)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:POP2(YNR052C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PSH1(YOL054W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PTH1(YHR189W)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RHR2(YIL053W)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL19B(YBL027W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT102(YGR275W)|FD-Score:-3.81|P-value:7.08E-5||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SEC72(YLR292C)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGS1(YMR190C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SKY1(YMR216C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLX9(YGR081C)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SLZ1(YNL196C_p)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SMP1(YBR182C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication Gene:SMY1(YKL079W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SOP4(YJL192C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRB8(YCR081W)|FD-Score:4.96|P-value:3.50E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRN2(YLR119W)|FD-Score:4.58|P-value:2.34E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SUS1(YBR111W-A)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWI3(YJL176C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TDA6(YPR157W_p)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:THI6(YPL214C)|FD-Score:3.79|P-value:7.47E-5||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TIF4631(YGR162W)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TMA19(YKL056C)|FD-Score:6.07|P-value:6.23E-10||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TPM1(YNL079C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:VAM6(YDL077C)|FD-Score:-4.32|P-value:7.87E-6||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA11(YPL234C)|FD-Score:-4.41|P-value:5.27E-6||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS28(YPL065W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS41(YDR080W)|FD-Score:-3.79|P-value:7.66E-5||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS61(YDR136C_d)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS9(YML097C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBT1(YLL048C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR417C(YDR417C_d)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YDR444W(YDR444W_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGL176C(YGL176C_p)|FD-Score:-3.78|P-value:7.94E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR122C-A(YGR122C-A_d)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YIL152W(YIL152W_p)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Putative protein of unknown function Gene:YLL007C(YLL007C_p)|FD-Score:-3.92|P-value:4.50E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YML047W-A(YML047W-A_d)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR119W-A(YMR119W-A_d)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR320W(YMR320W_d)|FD-Score:4.82|P-value:7.04E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL035C(YNL035C_p)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPL113C(YPL113C_p)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPL150W(YPL150W_p)|FD-Score:5.07|P-value:1.98E-7||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER012W5.374.01E-81.56PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YNL178W3.816.99E-50.38RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YMR028W3.432.99E-40.45TAP42Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
YDL102W2.980.001430.04POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YBR049C2.940.001640.02REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YKL052C2.920.001730.13ASK1Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
YCR057C2.790.002630.02PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YPR176C2.770.002800.05BET2Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YGR156W2.720.003270.04PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YDR327W_d2.680.003710.00YDR327W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
YNL272C2.670.003760.01SEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
YAL025C2.660.003900.07MAK16Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
YLR088W2.590.004730.04GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YEL058W2.560.005290.03PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YDL152W_d2.530.005700.06YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL056C6.076.23E-10TMA19Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress
YOR175C5.325.19E-8ALE1Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids
YKL079W5.181.11E-7SMY1Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins
YHR110W5.171.19E-7ERP5Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
YML097C5.161.22E-7VPS9A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
YNL196C_p5.091.77E-7SLZ1_pSporulation-specific protein with a leucine zipper motif
YPL150W_p5.071.98E-7YPL150W_pProtein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study
YCR081W4.963.50E-7SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YAL044C4.953.72E-7GCV3H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
YMR320W_d4.827.04E-7YMR320W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR119W4.582.34E-6SRN2Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
YLL026W4.552.62E-6HSP104Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
YGR162W4.434.71E-6TIF4631Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
YGL223C4.121.90E-5COG1Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YEL009C4.072.33E-5GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels

GO enrichment analysis for SGTC_3133
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0855.84E-11SGTC_2745rifampicin 24.3 μMMiscellaneous251414280.0977444
0.0587.44E-6SGTC_31169124943 49.5 μMChembridge (Drug-like library)419051950.0581395
0.0561.53E-5SGTC_31279126946 49.5 μMChembridge (Drug-like library)272432490.0657895ERG2
0.0534.31E-5SGTC_11780988-0033 191.0 μMChemDiv (Drug-like library)53922070.177215
0.0518.09E-5SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0641026Golgi
0.0518.87E-5SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.0898876NEO1-PIK1
0.0501.07E-4SGTC_32529135871 49.5 μMChembridge (Drug-like library)176828380.125
0.0501.25E-4SGTC_2741miltefosine 3.0 μMMiscellaneous35990.025974
0.0501.32E-4SGTC_29197978188 46.4 μMChembridge (Drug-like library)29802910.142857
0.0501.40E-4SGTC_31149122766 49.5 μMChembridge (Drug-like library)171769850.230769
0.0501.42E-4SGTC_30479092122 49.5 μMChembridge (Drug-like library)171738310.236842
0.0491.46E-4SGTC_1117retinoic acid 1.1 μMTimTec (Natural product library)4447950.0823529
0.0491.72E-4SGTC_1039k821-0043 94.0 μMChemDiv (Drug-like library)159927680.214286Golgi
0.0491.72E-4SGTC_1654st012436 41.8 μMTimTec (Natural product derivative library)43231320.116279
0.0491.79E-4SGTC_24885268135 45.7 μMMiscellaneous12703560.0641026

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3086911735049.47 μM0.3970592679265Chembridge (Drug-like library)365.3760432.27207
SGTC_2795778001054.55 μM0.378788953673Chembridge (Drug-like library)312.3812434.15113
SGTC_3285911281820.87 μM0.36986317017947Chembridge (Drug-like library)408.899043.29615cell wall
SGTC_3283910933235.82 μM0.35616417027281Chembridge (Drug-like library)329.82393.45913DNA intercalators
SGTC_3004907612271.43 μM0.34782644782551Chembridge (Drug-like library)267.322383.53612
SGTC_2802792581554.55 μM0.3424661262923Chembridge (Drug-like library)394.872463.07125
SGTC_2902578700660.46 μM0.318841591003Chembridge (Drug-like library)309.240013.32516Golgi
SGTC_2787528454071.43 μM0.318182736958Chembridge (Drug-like library)291.729563.24613
SGTC_20134024628200 μM0.317465230760Chembridge (Fragment library)226.273762.35422
SGTC_2892904735858.44 μM0.3142866466771Chembridge (Drug-like library)379.861122.414