9127742

4-(2,3-dihydro-1,4-benzodioxine-3-carbonyl)-N-(3,5-dimethoxyphenyl)piperazine-1-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3135
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 3643468
SMILES COC1=CC(=CC(=C1)NC(=O)N2CCN(CC2)C(=O)C3COC4=CC=CC=C4O3)OC
Standardized SMILES COc1cc(NC(=O)N2CCN(CC2)C(=O)C3COc4ccccc4O3)cc(OC)c1
Molecular weight 427.4504
ALogP 1.77
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.07
% growth inhibition (Hom. pool) -2.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3643468
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.26||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRR6(YGL247W)|FD-Score:-4.81|P-value:7.72E-7|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:DHR2(YKL078W)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.26||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FCF2(YLR051C)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.26||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:MCD4(YKL165C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.26||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NOP1(YDL014W)|FD-Score:-3.94|P-value:4.07E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:PRP31(YGR091W)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:ROK1(YGL171W)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.26||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPR2(YIR015W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.26||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:-3.45|P-value:2.80E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SSY5(YJL156C)|FD-Score:-6.16|P-value:3.69E-10|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:YKL083W(YKL083W_d)|FD-Score:3.98|P-value:3.50E-5|Clearance:0.26||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YPP1(YGR198W)|FD-Score:3.74|P-value:9.22E-5|Clearance:0.26||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:ARP9(YMR033W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.26||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRR6(YGL247W)|FD-Score:-4.81|P-value:7.72E-7|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:DHR2(YKL078W)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.26||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FCF2(YLR051C)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.26||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:MCD4(YKL165C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.26||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NOP1(YDL014W)|FD-Score:-3.94|P-value:4.07E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:PRP31(YGR091W)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:ROK1(YGL171W)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.26||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPR2(YIR015W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.26||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:-3.45|P-value:2.80E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SSY5(YJL156C)|FD-Score:-6.16|P-value:3.69E-10|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:YKL083W(YKL083W_d)|FD-Score:3.98|P-value:3.50E-5|Clearance:0.26||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YPP1(YGR198W)|FD-Score:3.74|P-value:9.22E-5|Clearance:0.26||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3643468
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AIM4(YBR194W)|FD-Score:8.15|P-value:1.84E-16||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ALB1(YJL122W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:APQ13(YJL075C_d)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARG5,6(YER069W)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARG7(YMR062C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATP10(YLR393W)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:CAT2(YML042W)|FD-Score:9.25|P-value:1.15E-20||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CCM1(YGR150C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCW12(YLR110C)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:DIC1(YLR348C)|FD-Score:4|P-value:3.19E-5||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:ENO1(YGR254W)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERP1(YAR002C-A)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:ERP3(YDL018C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FHN1(YGR131W)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FMP27(YLR454W_p)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCS1(YDL226C)|FD-Score:-3.8|P-value:7.28E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDB1(YPR184W)|FD-Score:-3.72|P-value:9.79E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:HAP3(YBL021C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HIS1(YER055C)|FD-Score:-4.38|P-value:5.92E-6||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HMF1(YER057C)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HSP82(YPL240C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:-9.17|P-value:2.30E-20||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:MON1(YGL124C)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL15(YLR312W-A)|FD-Score:4.31|P-value:8.11E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN5(YDR335W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MTC7(YEL033W_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:PER33(YLR064W)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PHO90(YJL198W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:POL32(YJR043C)|FD-Score:5.77|P-value:3.85E-9||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PPZ1(YML016C)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PYC2(YBR218C)|FD-Score:4.45|P-value:4.34E-6||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAD50(YNL250W)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RDI1(YDL135C)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:RNR4(YGR180C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPA14(YDR156W)|FD-Score:-4.48|P-value:3.78E-6||SGD DESC:RNA polymerase I subunit A14 Gene:RPL27A(YHR010W)|FD-Score:4.8|P-value:8.04E-7||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:SGS1(YMR190C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SHE9(YDR393W)|FD-Score:-3.89|P-value:5.03E-5||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SNF2(YOR290C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNU66(YOR308C)|FD-Score:5.91|P-value:1.70E-9||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SNX4(YJL036W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPT10(YJL127C)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRB2(YHR041C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRV2(YNL138W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SWI6(YLR182W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TIR1(YER011W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:UBP3(YER151C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:YBR226C(YBR226C_d)|FD-Score:4.86|P-value:5.97E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCR051W(YCR051W_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.73|P-value:9.48E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR514C(YDR514C)|FD-Score:5.38|P-value:3.68E-8||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YIL102C(YIL102C_p)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Putative protein of unknown function Gene:YJL181W(YJL181W_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YJL211C(YJL211C_d)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR334C(YLR334C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YMR172C-A(YMR172C-A_d)|FD-Score:5.33|P-value:5.00E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL010W(YNL010W_p)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL159C(YOL159C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA Gene:YOR072W(YOR072W_d)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL168W(YPL168W_p)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:ADE4(YMR300C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AIM4(YBR194W)|FD-Score:8.15|P-value:1.84E-16||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ALB1(YJL122W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:APQ13(YJL075C_d)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARG5,6(YER069W)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARG7(YMR062C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATP10(YLR393W)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:CAT2(YML042W)|FD-Score:9.25|P-value:1.15E-20||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CCM1(YGR150C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCW12(YLR110C)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:DIC1(YLR348C)|FD-Score:4|P-value:3.19E-5||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:ENO1(YGR254W)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERP1(YAR002C-A)|FD-Score:3.78|P-value:7.96E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:ERP3(YDL018C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FHN1(YGR131W)|FD-Score:3.99|P-value:3.26E-5||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FMP27(YLR454W_p)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCS1(YDL226C)|FD-Score:-3.8|P-value:7.28E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDB1(YPR184W)|FD-Score:-3.72|P-value:9.79E-5||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:HAP3(YBL021C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HIS1(YER055C)|FD-Score:-4.38|P-value:5.92E-6||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HMF1(YER057C)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HSP82(YPL240C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:-9.17|P-value:2.30E-20||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:MON1(YGL124C)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL15(YLR312W-A)|FD-Score:4.31|P-value:8.11E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN5(YDR335W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MTC7(YEL033W_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:PER33(YLR064W)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PHO90(YJL198W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:POL32(YJR043C)|FD-Score:5.77|P-value:3.85E-9||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PPZ1(YML016C)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PYC2(YBR218C)|FD-Score:4.45|P-value:4.34E-6||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAD50(YNL250W)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RDI1(YDL135C)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:RNR4(YGR180C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPA14(YDR156W)|FD-Score:-4.48|P-value:3.78E-6||SGD DESC:RNA polymerase I subunit A14 Gene:RPL27A(YHR010W)|FD-Score:4.8|P-value:8.04E-7||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:SGS1(YMR190C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SHE9(YDR393W)|FD-Score:-3.89|P-value:5.03E-5||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SNF2(YOR290C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNU66(YOR308C)|FD-Score:5.91|P-value:1.70E-9||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SNX4(YJL036W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPT10(YJL127C)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRB2(YHR041C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRV2(YNL138W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SWI6(YLR182W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TIR1(YER011W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:UBP3(YER151C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:YBR226C(YBR226C_d)|FD-Score:4.86|P-value:5.97E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCR051W(YCR051W_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.73|P-value:9.48E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR514C(YDR514C)|FD-Score:5.38|P-value:3.68E-8||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YIL102C(YIL102C_p)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Putative protein of unknown function Gene:YJL181W(YJL181W_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YJL211C(YJL211C_d)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR334C(YLR334C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YMR172C-A(YMR172C-A_d)|FD-Score:5.33|P-value:5.00E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL010W(YNL010W_p)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL159C(YOL159C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA Gene:YOR072W(YOR072W_d)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL168W(YPL168W_p)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL083W_d3.983.50E-50.26YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YGR198W3.749.22E-50.26YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YKL078W3.711.04E-40.26DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YGL171W3.581.72E-40.26ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YKL165C3.492.43E-40.26MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YMR033W3.393.55E-40.26ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YLR051C3.354.04E-40.26FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YIR015W3.324.57E-40.26RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YCL059C3.060.001110.01KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
YLR378C3.050.001150.02SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YJL195C_d3.030.001230.01YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YDR238C3.020.001250.07SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YDR045C2.960.001550.05RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YOR075W2.910.001830.04UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YGR030C2.870.002070.08POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML042W9.251.15E-20CAT2Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes
YBR194W8.151.84E-16AIM4Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress
YOR308C5.911.70E-9SNU66Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
YJR043C5.773.85E-9POL32Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
YDR514C5.383.68E-8YDR514CProtein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication
YMR172C-A_d5.335.00E-8YMR172C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YBR226C_d4.865.97E-7YBR226C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W
YHR010W4.808.04E-7RPL27ARibosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
YCR051W_p4.641.77E-6YCR051W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene
YJL075C_d4.592.22E-6APQ13_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate
YOR072W_d4.503.44E-6YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YGL124C4.493.52E-6MON1Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate
YBR218C4.454.34E-6PYC2Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
YLR312W-A4.318.11E-6MRPL15Mitochondrial ribosomal protein of the large subunit
YDL075W4.221.22E-5RPL31ARibosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3135
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1049.61E-16SGTC_31349127454 49.5 μMChembridge (Drug-like library)242628200.15493
0.0831.99E-10SGTC_31239126162 49.5 μMChembridge (Drug-like library)252398840.0909091
0.0647.65E-7SGTC_21956536160 144.2 μMChembridge (Fragment library)38343860.12307760S ribosome export
0.0647.92E-7SGTC_2673kobusone 100.0 μMMicrosource (Natural product library)67106760.0810811
0.0552.41E-5SGTC_15372',6'-dihydroxyflavanone 78.0 μMTimTec (Pure natural product library)51067870.166667
0.0543.83E-5SGTC_1817st052116 64.4 μMTimTec (Natural product derivative library)43613980.1RNA processing & uracil transport
0.0534.77E-5SGTC_9483448-0295 75.3 μMChemDiv (Drug-like library)6785690.0810811
0.0519.91E-5SGTC_13001094-0077 113.0 μMChemDiv (Drug-like library)55326360.156627
0.0501.15E-4SGTC_24285615643 7.8 μMMiscellaneous22530750.101124
0.0473.38E-4SGTC_32229128301 49.5 μMChembridge (Drug-like library)49113580.11904860S ribosome export
0.0464.15E-4SGTC_2654protoveratrine a 100.0 μMMicrosource (Natural product library)57022780.0714286
0.0464.47E-4SGTC_1838st055369 84.5 μMTimTec (Natural product derivative library)6760360.112676
0.0464.47E-4SGTC_30239089636 49.5 μMChembridge (Drug-like library)172492410.205128
0.0464.69E-4SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.162162
0.0455.25E-4SGTC_22647948757 110.8 μMChembridge (Fragment library)29714110.126761

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5313788-1495174 μM0.475412896655ChemDiv (Drug-like library)299.321182.37814unfolded protein response
SGTC_3531988-117558.3 μM0.3833332788357ChemDiv (Drug-like library)269.29522.39413unfolded protein response
SGTC_9073448-698675.5 μM0.319444590582ChemDiv (Drug-like library)352.307813.35126
SGTC_2892904735858.44 μM0.3088246466771Chembridge (Drug-like library)379.861122.414
SGTC_22957971645200 μM0.3050852978226Chembridge (Fragment library)204.268242.09911
SGTC_7033875-007571.8 μM0.2987012850814ChemDiv (Drug-like library)348.373642.05217
SGTC_2958907725416.72 μM0.29166716457351Chembridge (Drug-like library)292.33031.19815
SGTC_3164910070549.47 μM0.28985517174235Chembridge (Drug-like library)299.321182.8714
SGTC_2603homopterocarpin100 μM0.285714101795Microsource (Natural product library)284.306542.90804
SGTC_2847900566171.43 μM0.2835822991449Chembridge (Drug-like library)303.740263.42913