9095672

2-(4-chloro-3,5-dimethylphenoxy)-1-(4-pyridin-2-ylpiperazin-1-yl)propan-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3136
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17217380
SMILES CC1=CC(=CC(=C1Cl)C)OC(C)C(=O)N2CCN(CC2)C3=CC=CC=N3
Standardized SMILES CC(Oc1cc(C)c(Cl)c(C)c1)C(=O)N2CCN(CC2)c3ccccn3
Molecular weight 373.8765
ALogP 4.22
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.26
% growth inhibition (Hom. pool) 7.77


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17217380
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.19||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:BCP1(YDR361C)|FD-Score:3.29|P-value:4.97E-4|Clearance:0.13||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC1(YDR182W)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.08||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:DIS3(YOL021C)|FD-Score:-3.74|P-value:9.15E-5|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:FCP1(YMR277W)|FD-Score:3.16|P-value:7.96E-4|Clearance:0.17||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FRS2(YFL022C)|FD-Score:-3.51|P-value:2.27E-4|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:MCM7(YBR202W)|FD-Score:4.32|P-value:7.97E-6|Clearance:0.05||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NMD3(YHR170W)|FD-Score:-3.26|P-value:5.51E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RLI1(YDR091C)|FD-Score:-3.84|P-value:6.07E-5|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RNA15(YGL044C)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.04||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB7(YDR404C)|FD-Score:3.38|P-value:3.62E-4|Clearance:0.05||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC17(YJL011C)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPC53(YDL150W)|FD-Score:6.48|P-value:4.65E-11|Clearance:2.04||SGD DESC:RNA polymerase III subunit C53 Gene:RPF1(YHR088W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.02||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPP1(YHR062C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.48||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SPP2(YOR148C)|FD-Score:-3.69|P-value:1.12E-4|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:TFG1(YGR186W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.02||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TRS120(YDR407C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.06||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YLR458W(YLR458W_d)|FD-Score:4.44|P-value:4.45E-6|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:ATM1(YMR301C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.19||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:BCP1(YDR361C)|FD-Score:3.29|P-value:4.97E-4|Clearance:0.13||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC1(YDR182W)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.08||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:DIS3(YOL021C)|FD-Score:-3.74|P-value:9.15E-5|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:FCP1(YMR277W)|FD-Score:3.16|P-value:7.96E-4|Clearance:0.17||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FRS2(YFL022C)|FD-Score:-3.51|P-value:2.27E-4|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:MCM7(YBR202W)|FD-Score:4.32|P-value:7.97E-6|Clearance:0.05||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NMD3(YHR170W)|FD-Score:-3.26|P-value:5.51E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RLI1(YDR091C)|FD-Score:-3.84|P-value:6.07E-5|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RNA15(YGL044C)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.04||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB7(YDR404C)|FD-Score:3.38|P-value:3.62E-4|Clearance:0.05||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC17(YJL011C)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPC53(YDL150W)|FD-Score:6.48|P-value:4.65E-11|Clearance:2.04||SGD DESC:RNA polymerase III subunit C53 Gene:RPF1(YHR088W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.02||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPP1(YHR062C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.48||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SPP2(YOR148C)|FD-Score:-3.69|P-value:1.12E-4|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:TFG1(YGR186W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.02||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TRS120(YDR407C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.06||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YLR458W(YLR458W_d)|FD-Score:4.44|P-value:4.45E-6|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17217380
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:-4.77|P-value:9.12E-7||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ALF1(YNL148C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:API2(YDR525W_d)|FD-Score:5.06|P-value:2.08E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APS1(YLR170C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:BSC4(YNL269W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BTS1(YPL069C)|FD-Score:-5.57|P-value:1.28E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD23(YCR047C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CCW12(YLR110C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS7(YHR142W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COA6(YMR244C-A_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:CRS5(YOR031W)|FD-Score:4.31|P-value:7.98E-6||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CTF4(YPR135W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CTI6(YPL181W)|FD-Score:5.85|P-value:2.39E-9||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CYS3(YAL012W)|FD-Score:-5.38|P-value:3.66E-8||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DIA1(YMR316W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DOM34(YNL001W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:ECI1(YLR284C)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ELP2(YGR200C)|FD-Score:-4.42|P-value:4.93E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EMC3(YKL207W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:ERG2(YMR202W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERI1(YPL096C-A)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:GDT1(YBR187W_p)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GMC2(YLR445W)|FD-Score:5.98|P-value:1.08E-9||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GTT2(YLL060C)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GUP2(YPL189W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:GUT1(YHL032C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HPA2(YPR193C)|FD-Score:5.88|P-value:1.99E-9||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HSL7(YBR133C)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IRC20(YLR247C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:LAC1(YKL008C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LRP1(YHR081W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MAE1(YKL029C)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDM12(YOL009C)|FD-Score:-4.91|P-value:4.53E-7||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MDM35(YKL053C-A)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MGA2(YIR033W)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MON1(YGL124C)|FD-Score:-4.43|P-value:4.63E-6||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRE11(YMR224C)|FD-Score:-4.13|P-value:1.78E-5||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPS35(YGR165W)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRT4(YKL009W)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSA2(YKR077W)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:NEJ1(YLR265C)|FD-Score:-5.25|P-value:7.72E-8||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:NPT1(YOR209C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OAF1(YAL051W)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OM14(YBR230C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:PEX30(YLR324W)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO88(YBR106W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIG1(YLR273C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PKH3(YDR466W)|FD-Score:-5.22|P-value:8.79E-8||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PSY1(YKL076C_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:PTC6(YCR079W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RAD18(YCR066W)|FD-Score:5.36|P-value:4.17E-8||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RCK2(YLR248W)|FD-Score:3.86|P-value:5.56E-5||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RCO1(YMR075W)|FD-Score:3.73|P-value:9.41E-5||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:REC104(YHR157W)|FD-Score:-3.74|P-value:9.03E-5||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:REF2(YDR195W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RIF2(YLR453C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RNR4(YGR180C)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RRM3(YHR031C)|FD-Score:-4.04|P-value:2.73E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SAM3(YPL274W)|FD-Score:-3.24|P-value:5.96E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SHB17(YKR043C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHE1(YBL031W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHP1(YBL058W)|FD-Score:4.42|P-value:5.00E-6||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SLG1(YOR008C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SOD2(YHR008C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPO1(YNL012W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPO75(YLL005C)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SPT21(YMR179W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SRO7(YPR032W)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STF1(YDL130W-A)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:4.9|P-value:4.79E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA8(YAL064C-A_p)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:THI22(YPR121W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:TIP1(YBR067C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TLG2(YOL018C)|FD-Score:-4.87|P-value:5.71E-7||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA7(YLR262C-A)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TMN3(YER113C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation Gene:TRP3(YKL211C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TYW1(YPL207W)|FD-Score:4.87|P-value:5.66E-7||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:XRS2(YDR369C)|FD-Score:4.41|P-value:5.12E-6||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBT1(YLL048C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCR087W(YCR087W_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YEL045C(YEL045C_d)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YFL041W-A(YFL041W-A_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHR210C(YHR210C_p)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YJL120W(YJL120W_d)|FD-Score:-3.76|P-value:8.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR154W(YJR154W_p)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL169C(YKL169C_d)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YML012C-A(YML012C-A_d)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML037C(YML037C_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNL035C(YNL035C_p)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL194C(YNL194C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YOR072W(YOR072W_d)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR365C(YOR365C_p)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:5.52|P-value:1.69E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL080C(YPL080C_d)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL257W(YPL257W_p)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPL261C(YPL261C_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YSP1(YHR155W)|FD-Score:-3.84|P-value:6.22E-5||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ADA2(YDR448W)|FD-Score:-4.77|P-value:9.12E-7||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ALF1(YNL148C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:API2(YDR525W_d)|FD-Score:5.06|P-value:2.08E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APS1(YLR170C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:BSC4(YNL269W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BTS1(YPL069C)|FD-Score:-5.57|P-value:1.28E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD23(YCR047C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CCW12(YLR110C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS7(YHR142W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COA6(YMR244C-A_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:CRS5(YOR031W)|FD-Score:4.31|P-value:7.98E-6||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CTF4(YPR135W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CTI6(YPL181W)|FD-Score:5.85|P-value:2.39E-9||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CYS3(YAL012W)|FD-Score:-5.38|P-value:3.66E-8||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DIA1(YMR316W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DOM34(YNL001W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:ECI1(YLR284C)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ELP2(YGR200C)|FD-Score:-4.42|P-value:4.93E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EMC3(YKL207W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:ERG2(YMR202W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERI1(YPL096C-A)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:GDT1(YBR187W_p)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GMC2(YLR445W)|FD-Score:5.98|P-value:1.08E-9||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GTT2(YLL060C)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GUP2(YPL189W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:GUT1(YHL032C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HPA2(YPR193C)|FD-Score:5.88|P-value:1.99E-9||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HSL7(YBR133C)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IRC20(YLR247C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:LAC1(YKL008C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LRP1(YHR081W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MAE1(YKL029C)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDM12(YOL009C)|FD-Score:-4.91|P-value:4.53E-7||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MDM35(YKL053C-A)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MGA2(YIR033W)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MON1(YGL124C)|FD-Score:-4.43|P-value:4.63E-6||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRE11(YMR224C)|FD-Score:-4.13|P-value:1.78E-5||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPS35(YGR165W)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRT4(YKL009W)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSA2(YKR077W)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:NEJ1(YLR265C)|FD-Score:-5.25|P-value:7.72E-8||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:NPT1(YOR209C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OAF1(YAL051W)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OM14(YBR230C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:PEX30(YLR324W)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO88(YBR106W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIG1(YLR273C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PKH3(YDR466W)|FD-Score:-5.22|P-value:8.79E-8||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PSY1(YKL076C_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:PTC6(YCR079W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RAD18(YCR066W)|FD-Score:5.36|P-value:4.17E-8||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RCK2(YLR248W)|FD-Score:3.86|P-value:5.56E-5||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RCO1(YMR075W)|FD-Score:3.73|P-value:9.41E-5||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:REC104(YHR157W)|FD-Score:-3.74|P-value:9.03E-5||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:REF2(YDR195W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RIF2(YLR453C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RNR4(YGR180C)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RRM3(YHR031C)|FD-Score:-4.04|P-value:2.73E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SAM3(YPL274W)|FD-Score:-3.24|P-value:5.96E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SHB17(YKR043C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHE1(YBL031W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHP1(YBL058W)|FD-Score:4.42|P-value:5.00E-6||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SLG1(YOR008C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SOD2(YHR008C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPO1(YNL012W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPO75(YLL005C)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SPT21(YMR179W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SRO7(YPR032W)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STF1(YDL130W-A)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:4.9|P-value:4.79E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA8(YAL064C-A_p)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:THI22(YPR121W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:TIP1(YBR067C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TLG2(YOL018C)|FD-Score:-4.87|P-value:5.71E-7||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA7(YLR262C-A)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TMN3(YER113C)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation Gene:TRP3(YKL211C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TYW1(YPL207W)|FD-Score:4.87|P-value:5.66E-7||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:XRS2(YDR369C)|FD-Score:4.41|P-value:5.12E-6||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBT1(YLL048C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCR087W(YCR087W_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YEL045C(YEL045C_d)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YFL041W-A(YFL041W-A_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHR210C(YHR210C_p)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YJL120W(YJL120W_d)|FD-Score:-3.76|P-value:8.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR154W(YJR154W_p)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL169C(YKL169C_d)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YML012C-A(YML012C-A_d)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML037C(YML037C_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNL035C(YNL035C_p)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL194C(YNL194C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YOR072W(YOR072W_d)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR365C(YOR365C_p)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:5.52|P-value:1.69E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL080C(YPL080C_d)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL257W(YPL257W_p)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPL261C(YPL261C_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YSP1(YHR155W)|FD-Score:-3.84|P-value:6.22E-5||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL150W6.484.65E-112.04RPC53RNA polymerase III subunit C53
YLR458W_d4.444.45E-60.13YLR458W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis
YBR202W4.327.97E-60.05MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YDR182W4.261.01E-50.08CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
YHR062C4.181.43E-50.48RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR186W3.701.06E-40.02TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YJL011C3.681.16E-40.03RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YMR301C3.651.29E-40.19ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YHR088W3.462.66E-40.02RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YDR407C3.442.89E-40.06TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YDR404C3.383.62E-40.05RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YGL044C3.334.35E-40.04RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YDR361C3.294.97E-40.14BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YMR277W3.167.96E-40.17FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YDL064W2.990.001410.11UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR445W5.981.08E-9GMC2Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor
YPR193C5.881.99E-9HPA2Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity
YPL181W5.852.39E-9CTI6Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
YKL126W5.521.69E-8YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YCR066W5.364.17E-8RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YIR023W5.286.49E-8DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR525W_d5.062.08E-7API2_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
YBR069C4.904.79E-7TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YPL207W4.875.66E-7TYW1Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron
YNL148C4.721.19E-6ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YAL064C-A_p4.582.29E-6TDA8_pPutative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene
YPR032W4.562.55E-6SRO7Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
YLL005C4.464.19E-6SPO75Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis
YBL058W4.425.00E-6SHP1UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate
YDR369C4.415.12E-6XRS2Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling

GO enrichment analysis for SGTC_3136
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1194.01E-20SGTC_486niguldipine 82.0 μMMiscellaneous12360.153061amide catabolism
0.1165.38E-19SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.192771
0.1133.17E-18SGTC_31449087057 49.5 μMChembridge (Drug-like library)172496180.225352
0.0936.74E-13SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.131579amide catabolism
0.0931.04E-12SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.131868
0.0904.41E-12SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.146667
0.0896.16E-12SGTC_3234013-0906 34.3 μMChemDiv (Drug-like library)29100860.137931ERG2
0.0872.43E-11SGTC_31679103034 49.5 μMChembridge (Drug-like library)51278590.118421RPP1 & pyrimidine depletion
0.0822.83E-10SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.102273amide catabolism
0.0823.27E-10SGTC_5623772-2733 112.0 μMChemDiv (Drug-like library)28768740.123457ERG2
0.0814.52E-10SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.140625Golgi
0.0814.65E-10SGTC_12330325-0408 14.2 μMChemDiv (Drug-like library)128740.0819672
0.0814.73E-10SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.171053ERG2
0.0815.45E-10SGTC_32359132602 49.5 μMChembridge (Drug-like library)49122400.15
0.0809.21E-10SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.116883ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3160910021649.47 μM0.68421117191039Chembridge (Drug-like library)359.849883.73204
SGTC_2970909093841.86 μM0.67857117217104Chembridge (Drug-like library)339.43143.55304RNA processing & uracil transport
SGTC_3146909560949.47 μM0.66666717173999Chembridge (Drug-like library)380.268363.9104
SGTC_3150909757449.47 μM0.66071417217476Chembridge (Drug-like library)339.43143.52304
SGTC_3058909386149.47 μM0.62295117217477Chembridge (Drug-like library)373.876464.25504
SGTC_3028908777849.47 μM0.54838717217395Chembridge (Drug-like library)373.876464.04304
SGTC_3214534-389065.3 μM0.507937699746ChemDiv (Drug-like library)345.82333.24704RPP1 & pyrimidine depletion
SGTC_3056909490049.47 μM0.48333325236784Chembridge (Drug-like library)342.4072233.88404
SGTC_22267298446200 μM0.4745763283010Chembridge (Fragment library)287.379982.70104
SGTC_22867945697125 μM0.4666671245733Chembridge (Fragment library)287.379982.06904