9097027

(4-methyl-5-phenylthiophen-2-yl)-(4-methylpiperidin-1-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3147
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25236841
SMILES CC1CCN(CC1)C(=O)C2=CC(=C(S2)C3=CC=CC=C3)C
Standardized SMILES CC1CCN(CC1)C(=O)c2cc(C)c(s2)c3ccccc3
Molecular weight 299.4304
ALogP 4.66
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.49
% growth inhibition (Hom. pool) 5.55


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236841
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.04||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CAK1(YFL029C)|FD-Score:4.73|P-value:1.13E-6|Clearance:0.35||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT4(YDL143W)|FD-Score:-5.68|P-value:6.93E-9|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DBP5(YOR046C)|FD-Score:-3.4|P-value:3.33E-4|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:DIM1(YPL266W)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:MCD4(YKL165C)|FD-Score:7.98|P-value:7.54E-16|Clearance:2.1||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM3(YEL032W)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.14||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MIF2(YKL089W)|FD-Score:4.38|P-value:5.97E-6|Clearance:0.33||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:MOT1(YPL082C)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.08||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:MTR4(YJL050W)|FD-Score:3.91|P-value:4.63E-5|Clearance:0.01||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NSE3(YDR288W)|FD-Score:-3.25|P-value:5.67E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:ORC4(YPR162C)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.25||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PRT1(YOR361C)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.04||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RPS20(YHL015W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.05||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRN5(YLR141W)|FD-Score:-4.17|P-value:1.53E-5|Clearance:0||SGD DESC:Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p Gene:RRP12(YPL012W)|FD-Score:3.9|P-value:4.73E-5|Clearance:0||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:SLD5(YDR489W)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.04||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SPC42(YKL042W)|FD-Score:5.88|P-value:2.08E-9|Clearance:1.15||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SSL1(YLR005W)|FD-Score:3.84|P-value:6.11E-5|Clearance:0.1||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TFB2(YPL122C)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.04||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:UFD1(YGR048W)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.02||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:UTP14(YML093W)|FD-Score:3.15|P-value:8.10E-4|Clearance:0.01||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:WRS1(YOL097C)|FD-Score:3.14|P-value:8.38E-4|Clearance:0.11||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YPT1(YFL038C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.08||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:ALG14(YBR070C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.04||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CAK1(YFL029C)|FD-Score:4.73|P-value:1.13E-6|Clearance:0.35||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT4(YDL143W)|FD-Score:-5.68|P-value:6.93E-9|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DBP5(YOR046C)|FD-Score:-3.4|P-value:3.33E-4|Clearance:0||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:DIM1(YPL266W)|FD-Score:-3.42|P-value:3.14E-4|Clearance:0||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:MCD4(YKL165C)|FD-Score:7.98|P-value:7.54E-16|Clearance:2.1||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM3(YEL032W)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.14||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MIF2(YKL089W)|FD-Score:4.38|P-value:5.97E-6|Clearance:0.33||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:MOT1(YPL082C)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.08||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:MTR4(YJL050W)|FD-Score:3.91|P-value:4.63E-5|Clearance:0.01||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NSE3(YDR288W)|FD-Score:-3.25|P-value:5.67E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:ORC4(YPR162C)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.25||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PRT1(YOR361C)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.04||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RPS20(YHL015W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.05||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRN5(YLR141W)|FD-Score:-4.17|P-value:1.53E-5|Clearance:0||SGD DESC:Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p Gene:RRP12(YPL012W)|FD-Score:3.9|P-value:4.73E-5|Clearance:0||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:SLD5(YDR489W)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.04||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SPC42(YKL042W)|FD-Score:5.88|P-value:2.08E-9|Clearance:1.15||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SSL1(YLR005W)|FD-Score:3.84|P-value:6.11E-5|Clearance:0.1||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TFB2(YPL122C)|FD-Score:3.49|P-value:2.42E-4|Clearance:0.04||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:UFD1(YGR048W)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.02||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:UTP14(YML093W)|FD-Score:3.15|P-value:8.10E-4|Clearance:0.01||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:WRS1(YOL097C)|FD-Score:3.14|P-value:8.38E-4|Clearance:0.11||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YPT1(YFL038C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.08||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236841
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADE8(YDR408C)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AHT1(YHR093W_d)|FD-Score:3.81|P-value:6.97E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:COG7(YGL005C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRD1(YDL142C)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:CUL3(YGR003W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:DBP7(YKR024C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DIA4(YHR011W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:FAU1(YER183C)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FLO1(YAR050W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FMP41(YNL168C_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GBP2(YCL011C)|FD-Score:4|P-value:3.18E-5||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GSF2(YML048W)|FD-Score:5.64|P-value:8.58E-9||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GTT2(YLL060C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GZF3(YJL110C)|FD-Score:4.15|P-value:1.70E-5||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HMI1(YOL095C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IFA38(YBR159W)|FD-Score:4.38|P-value:5.89E-6||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:INO4(YOL108C)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:INP1(YMR204C)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC25(YLR021W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:ISC10(YER180C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:IST1(YNL265C)|FD-Score:8.83|P-value:5.18E-19||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:KAP122(YGL016W)|FD-Score:-3.83|P-value:6.31E-5||SGD DESC:Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance Gene:KAP123(YER110C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KNH1(YDL049C)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:KRE1(YNL322C)|FD-Score:4.29|P-value:8.78E-6||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LDB18(YLL049W)|FD-Score:6.97|P-value:1.57E-12||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:LYS4(YDR234W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MAE1(YKL029C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MET8(YBR213W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MIC14(YDR031W)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNT3(YIL014W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MSA2(YKR077W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MTM1(YGR257C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NDE1(YMR145C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NPP2(YEL016C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:PAC2(YER007W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PKH1(YDR490C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PML1(YLR016C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PPT1(YGR123C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:PRM6(YML047C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:RAD7(YJR052W)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RPE1(YJL121C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL18B(YNL301C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:5.02|P-value:2.57E-7||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTG1(YOL067C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SIC1(YLR079W)|FD-Score:-4.58|P-value:2.27E-6||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKI2(YLR398C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLF1(YDR515W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SNO2(YNL334C)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SOL1(YNR034W)|FD-Score:-3.96|P-value:3.73E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPS2(YDR522C)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SWI3(YJL176C)|FD-Score:-3.36|P-value:3.97E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:SYG1(YIL047C)|FD-Score:5.08|P-value:1.87E-7||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TOS1(YBR162C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRX1(YLR043C)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:VAB2(YEL005C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VPS74(YDR372C)|FD-Score:4.35|P-value:6.67E-6||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:XYL2(YLR070C)|FD-Score:6|P-value:9.92E-10||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBR285W(YBR285W_p)|FD-Score:7.81|P-value:2.81E-15||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCL012C(YCL012C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR199W(YDR199W_d)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YEF1(YEL041W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER097W(YER097W_d)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR146C-A(YGR146C-A_p)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:Putative protein of unknown function Gene:YIL002W-A(YIL002W-A_p)|FD-Score:-4.6|P-value:2.10E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL054W(YIL054W_p)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YJL045W(YJL045W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YJU3(YKL094W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKR005C(YKR005C_p)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Putative protein of unknown function Gene:YLR287C(YLR287C_p)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML003W(YML003W_p)|FD-Score:5.67|P-value:7.15E-9||SGD DESC:Putative protein of unknown function Gene:YMR172C-A(YMR172C-A_d)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL050C(YNL050C_p)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOR131C(YOR131C_p)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:ACM1(YPL267W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADE8(YDR408C)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AHT1(YHR093W_d)|FD-Score:3.81|P-value:6.97E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:COG7(YGL005C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRD1(YDL142C)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:CUL3(YGR003W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:DBP7(YKR024C)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DIA4(YHR011W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:FAU1(YER183C)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FLO1(YAR050W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FMP41(YNL168C_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GBP2(YCL011C)|FD-Score:4|P-value:3.18E-5||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GSF2(YML048W)|FD-Score:5.64|P-value:8.58E-9||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GTT2(YLL060C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GZF3(YJL110C)|FD-Score:4.15|P-value:1.70E-5||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HMI1(YOL095C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IFA38(YBR159W)|FD-Score:4.38|P-value:5.89E-6||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:INO4(YOL108C)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:INP1(YMR204C)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC25(YLR021W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:ISC10(YER180C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:IST1(YNL265C)|FD-Score:8.83|P-value:5.18E-19||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:KAP122(YGL016W)|FD-Score:-3.83|P-value:6.31E-5||SGD DESC:Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance Gene:KAP123(YER110C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KNH1(YDL049C)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:KRE1(YNL322C)|FD-Score:4.29|P-value:8.78E-6||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LDB18(YLL049W)|FD-Score:6.97|P-value:1.57E-12||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:LYS4(YDR234W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MAE1(YKL029C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MET8(YBR213W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MIC14(YDR031W)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNT3(YIL014W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MSA2(YKR077W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MTM1(YGR257C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NDE1(YMR145C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NPP2(YEL016C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:PAC2(YER007W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PKH1(YDR490C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PML1(YLR016C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PPT1(YGR123C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:PRM6(YML047C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family Gene:RAD7(YJR052W)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RPE1(YJL121C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL18B(YNL301C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:5.02|P-value:2.57E-7||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTG1(YOL067C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SIC1(YLR079W)|FD-Score:-4.58|P-value:2.27E-6||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKI2(YLR398C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLF1(YDR515W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SNO2(YNL334C)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SOL1(YNR034W)|FD-Score:-3.96|P-value:3.73E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPS2(YDR522C)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SWI3(YJL176C)|FD-Score:-3.36|P-value:3.97E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:SYG1(YIL047C)|FD-Score:5.08|P-value:1.87E-7||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TOS1(YBR162C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRX1(YLR043C)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:VAB2(YEL005C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VPS74(YDR372C)|FD-Score:4.35|P-value:6.67E-6||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:XYL2(YLR070C)|FD-Score:6|P-value:9.92E-10||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBR285W(YBR285W_p)|FD-Score:7.81|P-value:2.81E-15||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCL012C(YCL012C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR199W(YDR199W_d)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YEF1(YEL041W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER097W(YER097W_d)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR146C-A(YGR146C-A_p)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:Putative protein of unknown function Gene:YIL002W-A(YIL002W-A_p)|FD-Score:-4.6|P-value:2.10E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL054W(YIL054W_p)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YJL045W(YJL045W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YJU3(YKL094W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKR005C(YKR005C_p)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Putative protein of unknown function Gene:YLR287C(YLR287C_p)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML003W(YML003W_p)|FD-Score:5.67|P-value:7.15E-9||SGD DESC:Putative protein of unknown function Gene:YMR172C-A(YMR172C-A_d)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL050C(YNL050C_p)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOR131C(YOR131C_p)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL165C7.987.54E-162.10MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YKL042W5.882.08E-91.15SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YFL029C4.731.13E-60.35CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YKL089W4.385.97E-60.33MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
YEL032W4.052.57E-50.14MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YJL050W3.914.63E-50.01MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YPL012W3.904.73E-50.00RRP12Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
YOR361C3.904.77E-50.04PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YGR048W3.875.53E-50.02UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YLR005W3.846.11E-50.10SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YPL082C3.749.28E-50.08MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YBR070C3.651.29E-40.04ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YFL038C3.621.48E-40.08YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YHL015W3.542.01E-40.05RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YPL122C3.492.42E-40.04TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL265C8.835.18E-19IST1Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p
YBR285W_p7.812.81E-15YBR285W_pPutative protein of unknown function; YBR285W is not an essential gene
YLL049W6.971.57E-12LDB18Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24
YLR070C6.009.92E-10XYL2Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect
YML003W_p5.677.15E-9YML003W_pPutative protein of unknown function
YML048W5.648.58E-9GSF2ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression
YIL047C5.081.87E-7SYG1Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency
YFR031C-A5.022.57E-7RPL2ARibosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
YDL142C4.395.74E-6CRD1Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis
YBR159W4.385.89E-6IFA38Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
YDR372C4.356.67E-6VPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
YNL322C4.298.78E-6KRE1Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
YER180C4.211.30E-5ISC10Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells
YKL029C4.201.33E-5MAE1Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
YDR199W_d4.201.35E-5YDR199W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity

GO enrichment analysis for SGTC_3147
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1271.04E-22SGTC_31469095609 49.5 μMChembridge (Drug-like library)171739990.191781
0.0992.10E-14SGTC_31569099931 49.5 μMChembridge (Drug-like library)181110530.230769
0.0631.32E-6SGTC_2231micatex 6.9 μMMiscellaneous207370.0943396
0.0621.77E-6SGTC_60844-0013 19.8 μMChemDiv (Drug-like library)15849000.136364
0.0613.27E-6SGTC_30679117431 49.5 μMChembridge (Drug-like library)171508850.136364
0.0604.64E-6SGTC_13301469-0054 61.8 μMChemDiv (Drug-like library)71026640.13253iron homeostasis
0.0587.17E-6SGTC_1548st061468 90.8 μMTimTec (Pure natural product library)3907990.09375
0.0571.23E-5SGTC_23047443016 91.4 μMChembridge (Fragment library)9398540.102941
0.0571.31E-5SGTC_1777st045864 34.8 μMTimTec (Natural product derivative library)46914620.106667
0.0552.15E-5SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.259259
0.0552.28E-5SGTC_2413inermin 26.1 μMTimTec (Natural product derivative library)915100.0869565
0.0542.84E-5SGTC_2684capsaicin 100.0 μMMiscellaneous15489430.102564
0.0526.01E-5SGTC_9292805-0021 398.0 μMChemDiv (Drug-like library)30980240.107143
0.0526.94E-5SGTC_13853091-4834 14.1 μMChemDiv (Drug-like library)40728140.190476RPP1 & pyrimidine depletion
0.0501.30E-4SGTC_1907st060210 83.7 μMTimTec (Natural product derivative library)6887930.107692

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3026909063349.47 μM0.74468125236842Chembridge (Drug-like library)299.430444.72902
SGTC_3153910025849.47 μM0.68085125236848Chembridge (Drug-like library)315.429843.93603
SGTC_3038909129049.47 μM0.65306125236843Chembridge (Drug-like library)299.430444.78802
SGTC_2807794523851.95 μM0.58181817148797Chembridge (Drug-like library)366.476584.21604
SGTC_3024909063149.47 μM0.39655225236383Chembridge (Drug-like library)311.8021034.43603
SGTC_3057909594649.47 μM0.39655224540043Chembridge (Drug-like library)311.8021034.43603Golgi
SGTC_2985902237571.43 μM0.389834939335Chembridge (Drug-like library)350.454043.68812RPP1 & pyrimidine depletion
SGTC_3143908665849.47 μM0.38888925236626Chembridge (Drug-like library)300.41853.45803
SGTC_3149909785549.47 μM0.37525236625Chembridge (Drug-like library)365.287983.88103Golgi
SGTC_3021908765849.47 μM0.35714325237042Chembridge (Drug-like library)275.38593.58802