9100258

(2,6-dimethylmorpholin-4-yl)-(4-methyl-5-phenylthiophen-2-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3153
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25236848
SMILES CC1CN(CC(O1)C)C(=O)C2=CC(=C(S2)C3=CC=CC=C3)C
Standardized SMILES CC1CN(CC(C)O1)C(=O)c2cc(C)c(s2)c3ccccc3
Molecular weight 315.4298
ALogP 3.94
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.77
% growth inhibition (Hom. pool) 7.48


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236848
Download HIP data (tab-delimited text)  (excel)
Gene:CCL1(YPR025C)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:ERG13(YML126C)|FD-Score:-5.32|P-value:5.30E-8|Clearance:0||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:ERG20(YJL167W)|FD-Score:-3.31|P-value:4.72E-4|Clearance:0||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:FAP7(YDL166C)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FMN1(YDR236C)|FD-Score:-4.29|P-value:8.88E-6|Clearance:0||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GAB1(YLR459W)|FD-Score:-3.8|P-value:7.31E-5|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:LSM4(YER112W)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.44||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MRS6(YOR370C)|FD-Score:4|P-value:3.16E-5|Clearance:0.44||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:NUP145(YGL092W)|FD-Score:-3.28|P-value:5.27E-4|Clearance:0||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:NUP57(YGR119C)|FD-Score:4.04|P-value:2.68E-5|Clearance:0.44||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:POP6(YGR030C)|FD-Score:-3.86|P-value:5.62E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRI1(YIR008C)|FD-Score:4.53|P-value:2.92E-6|Clearance:0.44||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RSC58(YLR033W)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSE1(YML049C)|FD-Score:3.87|P-value:5.44E-5|Clearance:0.44||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:RTP1(YMR185W_p)|FD-Score:3.45|P-value:2.85E-4|Clearance:0.44||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SCC2(YDR180W)|FD-Score:4.81|P-value:7.66E-7|Clearance:0.44||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SPT16(YGL207W)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TIF35(YDR429C)|FD-Score:4.28|P-value:9.27E-6|Clearance:0.44||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YRB2(YIL063C)|FD-Score:3.76|P-value:8.48E-5|Clearance:0.44||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential Gene:CCL1(YPR025C)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:ERG13(YML126C)|FD-Score:-5.32|P-value:5.30E-8|Clearance:0||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:ERG20(YJL167W)|FD-Score:-3.31|P-value:4.72E-4|Clearance:0||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:FAP7(YDL166C)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FMN1(YDR236C)|FD-Score:-4.29|P-value:8.88E-6|Clearance:0||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GAB1(YLR459W)|FD-Score:-3.8|P-value:7.31E-5|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:LSM4(YER112W)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.44||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MRS6(YOR370C)|FD-Score:4|P-value:3.16E-5|Clearance:0.44||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:NUP145(YGL092W)|FD-Score:-3.28|P-value:5.27E-4|Clearance:0||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:NUP57(YGR119C)|FD-Score:4.04|P-value:2.68E-5|Clearance:0.44||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:POP6(YGR030C)|FD-Score:-3.86|P-value:5.62E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRI1(YIR008C)|FD-Score:4.53|P-value:2.92E-6|Clearance:0.44||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RSC58(YLR033W)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSE1(YML049C)|FD-Score:3.87|P-value:5.44E-5|Clearance:0.44||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:RTP1(YMR185W_p)|FD-Score:3.45|P-value:2.85E-4|Clearance:0.44||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SCC2(YDR180W)|FD-Score:4.81|P-value:7.66E-7|Clearance:0.44||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SPT16(YGL207W)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TIF35(YDR429C)|FD-Score:4.28|P-value:9.27E-6|Clearance:0.44||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YRB2(YIL063C)|FD-Score:3.76|P-value:8.48E-5|Clearance:0.44||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236848
Download HOP data (tab-delimited text)  (excel)
Gene:ABM1(YJR108W)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:AIM2(YAL049C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor Gene:AIM41(YOR215C)|FD-Score:-3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALO1(YML086C)|FD-Score:-3.75|P-value:8.95E-5||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:ASI1(YMR119W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:AUS1(YOR011W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:AVT3(YKL146W)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI4(YNL233W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BRE4(YDL231C)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:CCW12(YLR110C)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CIT1(YNR001C)|FD-Score:-3.94|P-value:4.02E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-5.42|P-value:3.03E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL81(YIR023W)|FD-Score:-3.77|P-value:8.29E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DNF2(YDR093W)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EAF1(YDR359C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EFT1(YOR133W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ELG1(YOR144C)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EOS1(YNL080C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERV41(YML067C)|FD-Score:-3.9|P-value:4.91E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:EXO5(YBR163W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FEN1(YCR034W)|FD-Score:3.8|P-value:7.37E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIR1(YER032W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FIT1(YDR534C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:HAC1(YFL031W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HBT1(YDL223C)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HER2(YMR293C)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HIM1(YDR317W)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Protein of unknown function involved in DNA repair Gene:HOC1(YJR075W)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HPA2(YPR193C)|FD-Score:4.93|P-value:4.04E-7||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HRB1(YNL004W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:IDP3(YNL009W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC2(YDR112W_d)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IST2(YBR086C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:ITR2(YOL103W)|FD-Score:-4.13|P-value:1.84E-5||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:IZH1(YDR492W)|FD-Score:-5.52|P-value:1.68E-8||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:IZH2(YOL002C)|FD-Score:-4.8|P-value:7.93E-7||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KEL1(YHR158C)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KNH1(YDL049C)|FD-Score:-4.75|P-value:9.96E-7||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:MAP1(YLR244C)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MIC14(YDR031W)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MIR1(YJR077C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MRPL7(YDR237W)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MSA1(YOR066W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSC7(YHR039C)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSG5(YNL053W)|FD-Score:-4.86|P-value:5.86E-7||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MUP1(YGR055W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NDT80(YHR124W)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NOP13(YNL175C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:ODC1(YPL134C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:PET20(YPL159C)|FD-Score:9.97|P-value:9.91E-24||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX5(YDR244W)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PGD1(YGL025C)|FD-Score:-3.97|P-value:3.60E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PLB1(YMR008C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PUF2(YPR042C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:QCR9(YGR183C)|FD-Score:4.43|P-value:4.70E-6||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RDL2(YOR286W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RPN4(YDL020C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM19(YNR037C)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RSM7(YJR113C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTG1(YOL067C)|FD-Score:3.77|P-value:8.00E-5||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTN1(YDR233C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RTS3(YGR161C_p)|FD-Score:5.59|P-value:1.14E-8||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SBA1(YKL117W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress Gene:SEC28(YIL076W)|FD-Score:4.02|P-value:2.87E-5||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SIW14(YNL032W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SKI2(YLR398C)|FD-Score:-4.55|P-value:2.69E-6||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SKN1(YGR143W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SKP2(YNL311C)|FD-Score:5.09|P-value:1.83E-7||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLM5(YCR024C)|FD-Score:-4.43|P-value:4.77E-6||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLO1(YER180C-A)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SOM1(YEL059C-A)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SSO2(YMR183C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:STR2(YJR130C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:SVS1(YPL163C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRK1(YJL129C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:VAC14(YLR386W)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAN1(YML115C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VMA4(YOR332W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VMS1(YDR049W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YBL095W(YBL095W_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR016W(YBR016W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR200W-A(YBR200W-A_p)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL068W(YDL068W_d)|FD-Score:8.31|P-value:4.77E-17||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL186W(YDL186W_p)|FD-Score:-3.18|P-value:7.24E-4||SGD DESC:Putative protein of unknown function; YDL186W is not an essential gene Gene:YDL241W(YDL241W_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR010C(YDR010C_d)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR306C(YDR306C_p)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YET3(YDL072C)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGR122W(YGR122W)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR139W(YGR139W_d)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR151C(YGR151C_d)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHL044W(YHL044W_p)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YHP1(YDR451C)|FD-Score:-3.86|P-value:5.56E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJR098C(YJR098C_p)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR012C(YLR012C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR072W(YLR072W_p)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR124W(YLR124W_d)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR252W(YLR252W_d)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR312C(YLR312C_p)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Putative protein of unknown function Gene:YLR352W(YLR352W_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene Gene:YMR102C(YMR102C_p)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNK1(YKL067W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YNL120C(YNL120C_d)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNR063W(YNR063W_p)|FD-Score:-3.31|P-value:4.58E-4||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR309C(YOR309C_d)|FD-Score:-4.34|P-value:7.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YOR318C(YOR318C_d)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPK1(YKL126W)|FD-Score:-5.24|P-value:8.01E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL067C(YPL067C_p)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL247C(YPL247C_p)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:ABM1(YJR108W)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:AIM2(YAL049C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor Gene:AIM41(YOR215C)|FD-Score:-3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALO1(YML086C)|FD-Score:-3.75|P-value:8.95E-5||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:ASI1(YMR119W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:AUS1(YOR011W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:AVT3(YKL146W)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI4(YNL233W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BRE4(YDL231C)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:CCW12(YLR110C)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CIT1(YNR001C)|FD-Score:-3.94|P-value:4.02E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-5.42|P-value:3.03E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL81(YIR023W)|FD-Score:-3.77|P-value:8.29E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DNF2(YDR093W)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EAF1(YDR359C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EFT1(YOR133W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ELG1(YOR144C)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EOS1(YNL080C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERV41(YML067C)|FD-Score:-3.9|P-value:4.91E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:EXO5(YBR163W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FEN1(YCR034W)|FD-Score:3.8|P-value:7.37E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIR1(YER032W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FIT1(YDR534C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:HAC1(YFL031W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HBT1(YDL223C)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HER2(YMR293C)|FD-Score:-4.11|P-value:1.98E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HIM1(YDR317W)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Protein of unknown function involved in DNA repair Gene:HOC1(YJR075W)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HPA2(YPR193C)|FD-Score:4.93|P-value:4.04E-7||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HRB1(YNL004W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:IDP3(YNL009W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC2(YDR112W_d)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IST2(YBR086C)|FD-Score:-3.8|P-value:7.29E-5||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:ITR2(YOL103W)|FD-Score:-4.13|P-value:1.84E-5||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:IZH1(YDR492W)|FD-Score:-5.52|P-value:1.68E-8||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc Gene:IZH2(YOL002C)|FD-Score:-4.8|P-value:7.93E-7||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KEL1(YHR158C)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KNH1(YDL049C)|FD-Score:-4.75|P-value:9.96E-7||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:MAP1(YLR244C)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MIC14(YDR031W)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MIR1(YJR077C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MRPL7(YDR237W)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MSA1(YOR066W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSC7(YHR039C)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSG5(YNL053W)|FD-Score:-4.86|P-value:5.86E-7||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MUP1(YGR055W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NDT80(YHR124W)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NOP13(YNL175C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:ODC1(YPL134C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:PET20(YPL159C)|FD-Score:9.97|P-value:9.91E-24||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX5(YDR244W)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PGD1(YGL025C)|FD-Score:-3.97|P-value:3.60E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PLB1(YMR008C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PUF2(YPR042C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:QCR9(YGR183C)|FD-Score:4.43|P-value:4.70E-6||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RDL2(YOR286W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RPN4(YDL020C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM19(YNR037C)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RSM7(YJR113C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTG1(YOL067C)|FD-Score:3.77|P-value:8.00E-5||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTN1(YDR233C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RTS3(YGR161C_p)|FD-Score:5.59|P-value:1.14E-8||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SBA1(YKL117W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress Gene:SEC28(YIL076W)|FD-Score:4.02|P-value:2.87E-5||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SIW14(YNL032W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SKI2(YLR398C)|FD-Score:-4.55|P-value:2.69E-6||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SKN1(YGR143W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SKP2(YNL311C)|FD-Score:5.09|P-value:1.83E-7||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLM5(YCR024C)|FD-Score:-4.43|P-value:4.77E-6||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLO1(YER180C-A)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SOM1(YEL059C-A)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SSO2(YMR183C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:STR2(YJR130C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:SVS1(YPL163C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRK1(YJL129C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:VAC14(YLR386W)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAN1(YML115C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VMA4(YOR332W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VMS1(YDR049W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YBL095W(YBL095W_p)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR016W(YBR016W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR200W-A(YBR200W-A_p)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL068W(YDL068W_d)|FD-Score:8.31|P-value:4.77E-17||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL186W(YDL186W_p)|FD-Score:-3.18|P-value:7.24E-4||SGD DESC:Putative protein of unknown function; YDL186W is not an essential gene Gene:YDL241W(YDL241W_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR010C(YDR010C_d)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR306C(YDR306C_p)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YET3(YDL072C)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGR122W(YGR122W)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR139W(YGR139W_d)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR151C(YGR151C_d)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHL044W(YHL044W_p)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YHP1(YDR451C)|FD-Score:-3.86|P-value:5.56E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJR098C(YJR098C_p)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR012C(YLR012C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR072W(YLR072W_p)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR124W(YLR124W_d)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR252W(YLR252W_d)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR312C(YLR312C_p)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Putative protein of unknown function Gene:YLR352W(YLR352W_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene Gene:YMR102C(YMR102C_p)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNK1(YKL067W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YNL120C(YNL120C_d)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNR063W(YNR063W_p)|FD-Score:-3.31|P-value:4.58E-4||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR309C(YOR309C_d)|FD-Score:-4.34|P-value:7.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YOR318C(YOR318C_d)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPK1(YKL126W)|FD-Score:-5.24|P-value:8.01E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL067C(YPL067C_p)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL247C(YPL247C_p)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR180W4.817.66E-70.44SCC2Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
YIR008C4.532.92E-60.44PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YDR429C4.289.27E-60.44TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YGR119C4.042.68E-50.44NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YOR370C4.003.16E-50.44MRS6Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
YER112W3.973.53E-50.44LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YML049C3.875.44E-50.44RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
YIL063C3.768.48E-50.44YRB2Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential
YMR185W_p3.452.85E-40.44RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YLL036C3.010.001320.08PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YJR022W2.930.001700.18LSM8Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
YPL169C2.750.002990.00MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YLR008C2.750.003009.24E-4PAM18Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
YHR170W2.750.003010.01NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YLR106C2.740.003060.02MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL159C9.979.91E-24PET20Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome
YDL068W_d8.314.77E-17YDL068W_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
YGR161C_p5.591.14E-8RTS3_pPutative component of the protein phosphatase type 2A complex
YOR144C5.161.26E-7ELG1Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
YNL311C5.091.83E-7SKP2F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
YPR193C4.934.04E-7HPA2Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity
YDR031W4.808.13E-7MIC14Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
YHR124W4.641.77E-6NDT80Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE)
YDR010C_d4.602.10E-6YDR010C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDL223C4.503.33E-6HBT1Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
YHR158C4.454.25E-6KEL1Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
YGR183C4.434.70E-6QCR9Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
YML115C4.211.27E-5VAN1Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
YJR075W4.171.49E-5HOC1Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
YKL067W4.092.17E-5YNK1Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_3153
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1211.04E-20SGTC_31579099274 49.5 μMChembridge (Drug-like library)172176620.164384
0.0961.68E-13SGTC_22077258803 200.0 μMChembridge (Fragment library)36667990.131148
0.0672.79E-7SGTC_32489135562 49.5 μMChembridge (Drug-like library)176507010.152778
0.0672.84E-7SGTC_31379097352 49.5 μMChembridge (Drug-like library)441204350.276923
0.0672.89E-7SGTC_20355154463 94.6 μMChembridge (Fragment library)27696230.2
0.0656.04E-7SGTC_2235salicylic acid 500.0 μMMiscellaneous3380.156863
0.0631.26E-6SGTC_33059127027 53.8 μMChembridge (Drug-like library)419052950.135135
0.0622.20E-6SGTC_24525863136 48.4 μMMiscellaneous21840960.115942
0.0612.43E-6SGTC_29339026794 32.5 μMChembridge (Drug-like library)175860270.133333
0.0613.21E-6SGTC_31589100007 49.5 μMChembridge (Drug-like library)192972330.212121
0.0561.43E-5SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.171875
0.0542.84E-5SGTC_33199138450 16.8 μMChembridge (Drug-like library)20296900.175
0.0543.39E-5SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.238806DNA intercalators
0.0543.41E-5SGTC_2687chenodiol 100.0 μMMicrosource (Natural product library)57021810.0740741
0.0543.69E-5SGTC_30399090270 49.5 μMChembridge (Drug-like library)171737500.132353RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3147909702749.47 μM0.68085125236841Chembridge (Drug-like library)299.430444.66202
SGTC_3026909063349.47 μM0.6625236842Chembridge (Drug-like library)299.430444.72902
SGTC_3038909129049.47 μM0.57692325236843Chembridge (Drug-like library)299.430444.78802
SGTC_2807794523851.95 μM0.5438617148797Chembridge (Drug-like library)366.476584.21604
SGTC_3030908761949.47 μM0.39622625236973Chembridge (Drug-like library)261.359323.08502
SGTC_3035909130349.47 μM0.38181825237002Chembridge (Drug-like library)275.38593.54102
SGTC_3143908665849.47 μM0.33333325236626Chembridge (Drug-like library)300.41853.45803
SGTC_3149909785549.47 μM0.32203425236625Chembridge (Drug-like library)365.287983.88103Golgi
SGTC_530973-002339.98 μM0.288136674768ChemDiv (Drug-like library)261.33943.3714RNA processing & uracil transport
SGTC_3044909319349.47 μM0.28571425236277Chembridge (Drug-like library)334.863563.54803