9100007

(3-bromophenyl)-[4-[(4-methylphenyl)methyl]piperazin-1-yl]methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3158
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 19297233
SMILES CC1=CC=C(C=C1)CN2CCN(CC2)C(=O)C3=CC(=CC=C3)Br
Standardized SMILES Cc1ccc(CN2CCN(CC2)C(=O)c3cccc(Br)c3)cc1
Molecular weight 373.2868
ALogP 4.02
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.17
% growth inhibition (Hom. pool) 1.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 19297233
Download HIP data (tab-delimited text)  (excel)
Gene:DHR2(YKL078W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.22||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FCF2(YLR051C)|FD-Score:6.77|P-value:6.34E-12|Clearance:1.24||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:MCM3(YEL032W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.04||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NUS1(YDL193W)|FD-Score:-5.08|P-value:1.87E-7|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRP38(YGR075C)|FD-Score:5.53|P-value:1.56E-8|Clearance:1.04||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RPC37(YKR025W)|FD-Score:-4.41|P-value:5.14E-6|Clearance:0||SGD DESC:RNA polymerase III subunit C37 Gene:RPC40(YPR110C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.12||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPS31(YLR167W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.57||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SPC42(YKL042W)|FD-Score:-3.81|P-value:7.08E-5|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPP2(YOR148C)|FD-Score:4.49|P-value:3.55E-6|Clearance:0.31||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SSL1(YLR005W)|FD-Score:3.86|P-value:5.60E-5|Clearance:0.16||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:YHR020W(YHR020W)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.1||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:DHR2(YKL078W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.22||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FCF2(YLR051C)|FD-Score:6.77|P-value:6.34E-12|Clearance:1.24||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:MCM3(YEL032W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.04||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NUS1(YDL193W)|FD-Score:-5.08|P-value:1.87E-7|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRP38(YGR075C)|FD-Score:5.53|P-value:1.56E-8|Clearance:1.04||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RPC37(YKR025W)|FD-Score:-4.41|P-value:5.14E-6|Clearance:0||SGD DESC:RNA polymerase III subunit C37 Gene:RPC40(YPR110C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.12||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPS31(YLR167W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.57||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SPC42(YKL042W)|FD-Score:-3.81|P-value:7.08E-5|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPP2(YOR148C)|FD-Score:4.49|P-value:3.55E-6|Clearance:0.31||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SSL1(YLR005W)|FD-Score:3.86|P-value:5.60E-5|Clearance:0.16||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:YHR020W(YHR020W)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.1||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 19297233
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHT1(YHR093W_d)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ARC18(YLR370C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO3(YDR035W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG10(YLL042C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:ATG36(YJL185C)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:BUD21(YOR078W)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CHD1(YER164W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:COA4(YLR218C)|FD-Score:-4.76|P-value:9.72E-7||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:DDI3(YNL335W_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DEG1(YFL001W)|FD-Score:5.76|P-value:4.25E-9||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DOS2(YDR068W)|FD-Score:-4.22|P-value:1.23E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EAF1(YDR359C)|FD-Score:5.05|P-value:2.21E-7||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EPS1(YIL005W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FAR3(YMR052W)|FD-Score:11.3|P-value:8.53E-30||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FOX2(YKR009C)|FD-Score:-4.86|P-value:6.01E-7||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:FUN26(YAL022C)|FD-Score:5.92|P-value:1.60E-9||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GEP3(YOR205C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GET2(YER083C)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GND1(YHR183W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HNT1(YDL125C)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:IRC16(YPR038W_d)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITC1(YGL133W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:JEM1(YJL073W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KAP123(YER110C)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LDH1(YBR204C)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:MAE1(YKL029C)|FD-Score:6.06|P-value:6.88E-10||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDM20(YOL076W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MMP1(YLL061W)|FD-Score:-3.84|P-value:6.25E-5||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MMS22(YLR320W)|FD-Score:4|P-value:3.20E-5||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MRP8(YKL142W)|FD-Score:5.12|P-value:1.56E-7||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MSH3(YCR092C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MSH6(YDR097C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:NDE1(YMR145C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NOP13(YNL175C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NUP53(YMR153W)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:ORM1(YGR038W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAU13(YHL046C)|FD-Score:-3.91|P-value:4.66E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PEX15(YOL044W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PMT7(YDR307W_p)|FD-Score:-8.8|P-value:7.01E-19||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PTH2(YBL057C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RGT1(YKL038W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:ROX3(YBL093C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL42B(YHR141C)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:SCP1(YOR367W)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SHE4(YOR035C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SRO77(YBL106C)|FD-Score:4.4|P-value:5.43E-6||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SVS1(YPL163C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:SYH1(YPL105C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBP16(YPL072W)|FD-Score:3.96|P-value:3.83E-5||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UBX7(YBR273C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:YAP1802(YGR241C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YCR022C(YCR022C_d)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDL068W(YDL068W_d)|FD-Score:6.58|P-value:2.40E-11||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL162C(YDL162C_d)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEF1(YEL041W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YFT2(YDR319C_p)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YHR095W(YHR095W_d)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL162C-A(YKL162C-A_d)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML018C(YML018C_p)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YNL195C(YNL195C_p)|FD-Score:-5.24|P-value:7.99E-8||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR123C(YPR123C_d)|FD-Score:5.61|P-value:9.88E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:AFT1(YGL071W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHT1(YHR093W_d)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ARC18(YLR370C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO3(YDR035W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG10(YLL042C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:ATG36(YJL185C)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:BUD21(YOR078W)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CHD1(YER164W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:COA4(YLR218C)|FD-Score:-4.76|P-value:9.72E-7||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:DDI3(YNL335W_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DEG1(YFL001W)|FD-Score:5.76|P-value:4.25E-9||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DOS2(YDR068W)|FD-Score:-4.22|P-value:1.23E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EAF1(YDR359C)|FD-Score:5.05|P-value:2.21E-7||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EPS1(YIL005W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FAR3(YMR052W)|FD-Score:11.3|P-value:8.53E-30||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FOX2(YKR009C)|FD-Score:-4.86|P-value:6.01E-7||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:FUN26(YAL022C)|FD-Score:5.92|P-value:1.60E-9||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GEP3(YOR205C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GET2(YER083C)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GND1(YHR183W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HNT1(YDL125C)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:IRC16(YPR038W_d)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITC1(YGL133W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:JEM1(YJL073W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KAP123(YER110C)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LDH1(YBR204C)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:MAE1(YKL029C)|FD-Score:6.06|P-value:6.88E-10||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDM20(YOL076W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MMP1(YLL061W)|FD-Score:-3.84|P-value:6.25E-5||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MMS22(YLR320W)|FD-Score:4|P-value:3.20E-5||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MRP8(YKL142W)|FD-Score:5.12|P-value:1.56E-7||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MSH3(YCR092C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MSH6(YDR097C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:NDE1(YMR145C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NOP13(YNL175C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NUP53(YMR153W)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:ORM1(YGR038W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAU13(YHL046C)|FD-Score:-3.91|P-value:4.66E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PEX15(YOL044W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PMT7(YDR307W_p)|FD-Score:-8.8|P-value:7.01E-19||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PTH2(YBL057C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RGT1(YKL038W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:ROX3(YBL093C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL42B(YHR141C)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:SCP1(YOR367W)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SHE4(YOR035C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SRO77(YBL106C)|FD-Score:4.4|P-value:5.43E-6||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SVS1(YPL163C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:SYH1(YPL105C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBP16(YPL072W)|FD-Score:3.96|P-value:3.83E-5||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UBX7(YBR273C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:YAP1802(YGR241C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YCR022C(YCR022C_d)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDL068W(YDL068W_d)|FD-Score:6.58|P-value:2.40E-11||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL162C(YDL162C_d)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEF1(YEL041W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YFT2(YDR319C_p)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YHR095W(YHR095W_d)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL162C-A(YKL162C-A_d)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML018C(YML018C_p)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YNL195C(YNL195C_p)|FD-Score:-5.24|P-value:7.99E-8||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR123C(YPR123C_d)|FD-Score:5.61|P-value:9.88E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR051C6.776.34E-121.24FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YGR075C5.531.56E-81.04PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly
YOR148C4.493.55E-60.31SPP2Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
YKL078W4.181.47E-50.22DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YHR020W3.963.73E-50.10YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YLR005W3.865.60E-50.16SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YPR110C3.701.06E-40.12RPC40RNA polymerase subunit AC40, common to RNA polymerase I and III
YEL032W3.591.67E-40.04MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YLR167W3.541.97E-40.57RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YIL150C2.980.001460.03MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YGR119C2.950.001610.04NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YDR177W2.910.001830.10UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YPL237W2.800.002530.11SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YDL015C2.700.003510.05TSC13Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
YOL097C2.650.004050.05WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR052W11.308.53E-30FAR3Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YDL068W_d6.582.40E-11YDL068W_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
YKL029C6.066.88E-10MAE1Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
YAL022C5.921.60E-9FUN26Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis
YFL001W5.764.25E-9DEG1tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
YPR123C_d5.619.88E-9YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YKL142W5.121.56E-7MRP8Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis
YDR359C5.052.21E-7EAF1Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1
YDL162C_d4.751.00E-6YDL162C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly
YPL163C4.691.34E-6SVS1Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication
YGL071W4.444.51E-6AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YBL106C4.405.43E-6SRO77Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
YER164W4.261.01E-5CHD1Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes
YOR035C4.211.29E-5SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YEL041W4.141.75E-5YEF1ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3158
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0765.55E-9SGTC_30959117207 49.5 μMChembridge (Drug-like library)170152290.162162
0.0758.26E-9SGTC_31489097893 49.5 μMChembridge (Drug-like library)171741000.133333
0.0723.41E-8SGTC_30869117350 49.5 μMChembridge (Drug-like library)26792650.166667
0.0691.35E-7SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.0895522
0.0649.22E-7SGTC_31599100096 49.5 μMChembridge (Drug-like library)252368750.241935
0.0649.93E-7SGTC_14801070-0044 41.2 μMChemDiv (Drug-like library)31249870.142857
0.0631.28E-6SGTC_6721441-0018 53.7 μMChemDiv (Drug-like library)2511410.0634921
0.0631.37E-6SGTC_12310242-0792 125.0 μMChemDiv (Drug-like library)37076350.107143
0.0613.10E-6SGTC_1941486-1323 56.9 μMChemDiv (Drug-like library)51787540.162162
0.0613.21E-6SGTC_31539100258 49.5 μMChembridge (Drug-like library)252368480.212121
0.0603.70E-6SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0869565
0.0603.90E-6SGTC_14234048-0112 5.6 μMChemDiv (Drug-like library)X14230.207792
0.0596.56E-6SGTC_790052-0007 94.1 μMChemDiv (Drug-like library)28014720.180328
0.0561.73E-5SGTC_1830st055982 50.3 μMTimTec (Natural product derivative library)4553130.0793651
0.0561.83E-5SGTC_9423253-1115 227.0 μMChemDiv (Drug-like library)464978970.205882

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3049909222349.47 μM0.5882358623917Chembridge (Drug-like library)328.835843.93502
SGTC_3054909439749.47 μM0.522598354Chembridge (Drug-like library)330.422883.69302fatty acid desaturase (OLE1)
SGTC_3041909192949.47 μM0.50943425236887Chembridge (Drug-like library)328.471664.03603redox potentiating
SGTC_5733702-055384.7 μM0.4883721222756ChemDiv (Drug-like library)424.17284.94102
SGTC_13752910-0797186 μM0.481481742508ChemDiv (Drug-like library)340.416162.75204
SGTC_7294092-058594.1 μM0.4655172169061ChemDiv (Drug-like library)390.4500234.5504
SGTC_11594092-053861.2 μM0.461538717301ChemDiv (Drug-like library)332.7997233.65403
SGTC_5423381-024489.3 μM0.456141112843ChemDiv (Drug-like library)360.448863.67603
SGTC_3040909189449.47 μM0.4561425236966Chembridge (Drug-like library)328.471663.71503DNA intercalators
SGTC_14554262-0120131 μM0.4545451529587ChemDiv (Drug-like library)308.460424.87302plasma membrane duress