9103054

N-(4-fluorophenyl)-2-pyridin-4-ylquinoline-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3170
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 16432970
SMILES C1=CC=C2C(=C1)C(=CC(=N2)C3=CC=NC=C3)C(=O)NC4=CC=C(C=C4)F
Standardized SMILES Fc1ccc(NC(=O)c2cc(nc3ccccc23)c4ccncc4)cc1
Molecular weight 343.3538
ALogP 3.8
H-bond donor count 1
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.93
% growth inhibition (Hom. pool) 5.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16432970
Download HIP data (tab-delimited text)  (excel)
Gene:ARC35(YNR035C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.1||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CAK1(YFL029C)|FD-Score:-3.37|P-value:3.74E-4|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCA1(YER168C)|FD-Score:-3.29|P-value:5.06E-4|Clearance:0||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CSL4(YNL232W)|FD-Score:-4.29|P-value:8.82E-6|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:DCP1(YOL149W)|FD-Score:4.36|P-value:6.62E-6|Clearance:0.85||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DPS1(YLL018C)|FD-Score:3.22|P-value:6.34E-4|Clearance:0.12||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:ENP1(YBR247C)|FD-Score:-3.95|P-value:3.83E-5|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:LSG1(YGL099W)|FD-Score:9.38|P-value:3.30E-21|Clearance:4.03||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM2(YBL026W)|FD-Score:-4.15|P-value:1.67E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCM10(YIL150C)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.06||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MES1(YGR264C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NMD3(YHR170W)|FD-Score:10.5|P-value:2.97E-26|Clearance:4.03||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:3.39|P-value:3.49E-4|Clearance:0.06||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:PBN1(YCL052C)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PDI1(YCL043C)|FD-Score:-3.23|P-value:6.23E-4|Clearance:0||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PRP28(YDR243C)|FD-Score:5.34|P-value:4.71E-8|Clearance:0.98||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PWP2(YCR057C)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPT1(YKL145W)|FD-Score:-3.26|P-value:5.53E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSC9(YML127W)|FD-Score:5.34|P-value:4.52E-8|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC26(YDR238C)|FD-Score:-4.27|P-value:9.67E-6|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SSU72(YNL222W)|FD-Score:-3.6|P-value:1.59E-4|Clearance:0||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:SUI2(YJR007W)|FD-Score:-3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TIF6(YPR016C)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.12||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:YLR076C(YLR076C_d)|FD-Score:-4.8|P-value:8.12E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPL142C(YPL142C_d)|FD-Score:-4.77|P-value:9.03E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:ARC35(YNR035C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.1||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CAK1(YFL029C)|FD-Score:-3.37|P-value:3.74E-4|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCA1(YER168C)|FD-Score:-3.29|P-value:5.06E-4|Clearance:0||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CSL4(YNL232W)|FD-Score:-4.29|P-value:8.82E-6|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:DCP1(YOL149W)|FD-Score:4.36|P-value:6.62E-6|Clearance:0.85||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DPS1(YLL018C)|FD-Score:3.22|P-value:6.34E-4|Clearance:0.12||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:ENP1(YBR247C)|FD-Score:-3.95|P-value:3.83E-5|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:LSG1(YGL099W)|FD-Score:9.38|P-value:3.30E-21|Clearance:4.03||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM2(YBL026W)|FD-Score:-4.15|P-value:1.67E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCM10(YIL150C)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.06||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MES1(YGR264C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NMD3(YHR170W)|FD-Score:10.5|P-value:2.97E-26|Clearance:4.03||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:3.39|P-value:3.49E-4|Clearance:0.06||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:PBN1(YCL052C)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PDI1(YCL043C)|FD-Score:-3.23|P-value:6.23E-4|Clearance:0||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PRP28(YDR243C)|FD-Score:5.34|P-value:4.71E-8|Clearance:0.98||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PWP2(YCR057C)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPT1(YKL145W)|FD-Score:-3.26|P-value:5.53E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSC9(YML127W)|FD-Score:5.34|P-value:4.52E-8|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC26(YDR238C)|FD-Score:-4.27|P-value:9.67E-6|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SSU72(YNL222W)|FD-Score:-3.6|P-value:1.59E-4|Clearance:0||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:SUI2(YJR007W)|FD-Score:-3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TIF6(YPR016C)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.12||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:YLR076C(YLR076C_d)|FD-Score:-4.8|P-value:8.12E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPL142C(YPL142C_d)|FD-Score:-4.77|P-value:9.03E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16432970
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:-5.61|P-value:1.04E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC2(YCR082W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ARO1(YDR127W)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATO2(YNR002C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BEM4(YPL161C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUD16(YEL029C)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:COQ8(YGL119W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:CRP1(YHR146W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTF8(YHR191C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DIG1(YPL049C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DUS4(YLR405W)|FD-Score:-4.79|P-value:8.42E-7||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ELP3(YPL086C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:EMC1(YCL045C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:END3(YNL084C)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FCJ1(YKR016W)|FD-Score:4.95|P-value:3.64E-7||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FUR4(YBR021W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:HBN1(YCL026C-B_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HPA2(YPR193C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HST1(YOL068C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:INO1(YJL153C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IST1(YNL265C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:KIP2(YPL155C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:MBR1(YKL093W)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants Gene:MET16(YPR167C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MKK1(YOR231W)|FD-Score:-5.9|P-value:1.78E-9||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MRPL8(YJL063C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NGG1(YDR176W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PET10(YKR046C)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX14(YGL153W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PGM3(YMR278W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PSY3(YLR376C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PTP2(YOR208W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RAD5(YLR032W)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAV1(YJR033C)|FD-Score:-3.65|P-value:1.34E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RDI1(YDL135C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:REG1(YDR028C)|FD-Score:-4.46|P-value:4.03E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REX3(YLR107W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases Gene:RIM1(YCR028C-A)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPL36B(YPL249C-A)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RUD3(YOR216C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAT4(YCR008W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SGF29(YCL010C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SSD1(YDR293C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUM1(YDR310C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TBS1(YBR150C)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TDA5(YLR426W_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:THI21(YPL258C)|FD-Score:7.03|P-value:1.01E-12||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:THP2(YHR167W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TRE1(YPL176C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:-4.66|P-value:1.60E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA4(YHR111W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:YDR514C(YDR514C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YEL043W(YEL043W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YGR121W-A(YGR121W-A_p)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Putative protein of unknown function Gene:YGR269W(YGR269W_d)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YJL206C(YJL206C_p)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YLR366W(YLR366W_d)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function Gene:YMR245W(YMR245W_d)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL034W(YNL034W_p)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YNR029C(YNR029C_p)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR068C(YNR068C_p)|FD-Score:4.77|P-value:9.18E-7||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YOR072W(YOR072W_d)|FD-Score:5.34|P-value:4.56E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR223W(YOR223W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOR248W(YOR248W_d)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR063C(YPR063C_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:ER-localized protein of unknown function Gene:ZAP1(YJL056C)|FD-Score:-4.65|P-value:1.65E-6||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ZRG8(YER033C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:AFT1(YGL071W)|FD-Score:-5.61|P-value:1.04E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC2(YCR082W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ARO1(YDR127W)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATO2(YNR002C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BEM4(YPL161C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUD16(YEL029C)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:COQ8(YGL119W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:CRP1(YHR146W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTF8(YHR191C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DIG1(YPL049C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DUS4(YLR405W)|FD-Score:-4.79|P-value:8.42E-7||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ELP3(YPL086C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:EMC1(YCL045C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:END3(YNL084C)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FCJ1(YKR016W)|FD-Score:4.95|P-value:3.64E-7||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FUR4(YBR021W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:HBN1(YCL026C-B_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HPA2(YPR193C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HST1(YOL068C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:INO1(YJL153C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IST1(YNL265C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:KIP2(YPL155C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:MBR1(YKL093W)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants Gene:MET16(YPR167C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MKK1(YOR231W)|FD-Score:-5.9|P-value:1.78E-9||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MRPL8(YJL063C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NGG1(YDR176W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PET10(YKR046C)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX14(YGL153W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PGM3(YMR278W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PSY3(YLR376C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PTP2(YOR208W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RAD5(YLR032W)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAV1(YJR033C)|FD-Score:-3.65|P-value:1.34E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RDI1(YDL135C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:REG1(YDR028C)|FD-Score:-4.46|P-value:4.03E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REX3(YLR107W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases Gene:RIM1(YCR028C-A)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPL36B(YPL249C-A)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RUD3(YOR216C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAT4(YCR008W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SGF29(YCL010C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SSD1(YDR293C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUM1(YDR310C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TBS1(YBR150C)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TDA5(YLR426W_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:THI21(YPL258C)|FD-Score:7.03|P-value:1.01E-12||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:THP2(YHR167W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TRE1(YPL176C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:-4.66|P-value:1.60E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA4(YHR111W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:YDR514C(YDR514C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YEL043W(YEL043W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YGR121W-A(YGR121W-A_p)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Putative protein of unknown function Gene:YGR269W(YGR269W_d)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YJL206C(YJL206C_p)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YLR366W(YLR366W_d)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function Gene:YMR245W(YMR245W_d)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL034W(YNL034W_p)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YNR029C(YNR029C_p)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR068C(YNR068C_p)|FD-Score:4.77|P-value:9.18E-7||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YOR072W(YOR072W_d)|FD-Score:5.34|P-value:4.56E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR223W(YOR223W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOR248W(YOR248W_d)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR063C(YPR063C_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:ER-localized protein of unknown function Gene:ZAP1(YJL056C)|FD-Score:-4.65|P-value:1.65E-6||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ZRG8(YER033C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W10.502.97E-264.03NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGL099W9.383.30E-214.03LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YML127W5.344.52E-80.01RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YDR243C5.344.71E-80.98PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YOL149W4.366.62E-60.85DCP1Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
YPR016C3.512.24E-40.12TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YOR206W3.393.49E-40.06NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YNR035C3.334.40E-40.10ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YLL018C3.226.34E-40.12DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YIL150C3.109.67E-40.06MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YNL221C3.040.001170.08POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YDR361C2.970.001490.02BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YDR460W2.950.001570.03TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YDL014W2.920.001730.03NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YKL210W2.900.001890.14UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL258C7.031.01E-12THI21Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p
YOR072W_d5.344.56E-8YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YKR016W4.953.64E-7FCJ1Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane
YCR031C4.943.95E-7RPS14AProtein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication
YNR068C_p4.779.18E-7YNR068C_pPutative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form
YNL084C4.751.02E-6END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YGR121W-A_p4.691.37E-6YGR121W-A_pPutative protein of unknown function
YLR032W4.425.04E-6RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YGL153W4.082.26E-5PEX14Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p
YOL068C4.072.33E-5HST1NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YGL119W3.875.48E-5COQ8Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
YCR082W3.875.53E-5AHC2Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex
YMR245W_d3.865.59E-5YMR245W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR150C3.846.26E-5TBS1Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication
YDR127W3.739.61E-5ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids

GO enrichment analysis for SGTC_3170
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2245.61E-68SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.18309960S ribosome export
0.2181.99E-64SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.20270360S ribosome export
0.2056.02E-57SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.15714360S ribosome export
0.1939.84E-51SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.13483160S ribosome export
0.1906.15E-49SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.11904860S ribosome export
0.1824.42E-45SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.14492860S ribosome export
0.1741.46E-41SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.10144960S ribosome export
0.1626.96E-36SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.059523860S ribosome export
0.1599.18E-35SGTC_6450845-0943 5.4 μMChemDiv (Drug-like library)18851060.15384660S ribosome export
0.1591.02E-34SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.082191860S ribosome export
0.1574.77E-34SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.065217460S ribosome export
0.1579.64E-34SGTC_31689103419 49.5 μMChembridge (Drug-like library)166492560.22535260S ribosome export
0.1532.40E-32SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.089743660S ribosome export
0.1521.02E-31SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.059523860S ribosome export
0.1506.24E-31SGTC_1350933-0004 26.3 μMChemDiv (Drug-like library)212306170.13888960S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21245246320200 μM0.469388579342Chembridge (Fragment library)216.2110631.66913heme biosynthesis & mitochondrial translocase
SGTC_22897946404165.07 μM0.3921571246120Chembridge (Fragment library)234.2015261.87514
SGTC_3337914434233.55 μM0.38095217682949Chembridge (Drug-like library)313.3493832.62415
SGTC_2872904403725.97 μM0.370376465195Chembridge (Drug-like library)284.1131234.14912
SGTC_20485246248200 μM0.363636677046Chembridge (Fragment library)216.2110631.66913
SGTC_2904722513542.03 μM0.3636364446116Chembridge (Drug-like library)364.437564.06914
SGTC_4253825-701713.1 μM0.3548393397440ChemDiv (Drug-like library)329.3702435.78914
SGTC_2864903475547.6 μM0.343754930451Chembridge (Drug-like library)361.393862.58124
SGTC_3070911700549.47 μM0.3333338779016Chembridge (Drug-like library)273.3021433.5313
SGTC_2942905277762.25 μM0.3275866469503Chembridge (Drug-like library)284.1131234.14912