9107506

N-(3-chloro-4-methylphenyl)-2-(3,4-dihydro-1H-isoquinolin-2-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3177
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 8897194
SMILES CC1=C(C=C(C=C1)NC(=O)CN2CCC3=CC=CC=C3C2)Cl
Standardized SMILES Cc1ccc(NC(=O)CN2CCc3ccccc3C2)cc1Cl
Molecular weight 314.8093
ALogP 3.77
H-bond donor count 1
H-bond acceptor count 2
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.58
% growth inhibition (Hom. pool) 12.07


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 8897194
Download HIP data (tab-delimited text)  (excel)
Gene:APC1(YNL172W)|FD-Score:3.24|P-value:6.02E-4|Clearance:0.17||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:GUK1(YDR454C)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.37||SGD DESC:Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins Gene:LCB2(YDR062W)|FD-Score:-3.29|P-value:4.93E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:SEC26(YDR238C)|FD-Score:-4.95|P-value:3.67E-7|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TAF12(YDR145W)|FD-Score:3.65|P-value:1.34E-4|Clearance:0.37||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TOM40(YMR203W)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:APC1(YNL172W)|FD-Score:3.24|P-value:6.02E-4|Clearance:0.17||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:GUK1(YDR454C)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.37||SGD DESC:Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins Gene:LCB2(YDR062W)|FD-Score:-3.29|P-value:4.93E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:SEC26(YDR238C)|FD-Score:-4.95|P-value:3.67E-7|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TAF12(YDR145W)|FD-Score:3.65|P-value:1.34E-4|Clearance:0.37||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TOM40(YMR203W)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 8897194
Download HOP data (tab-delimited text)  (excel)
Gene:BNA4(YBL098W)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:ELA1(YNL230C)|FD-Score:-3.76|P-value:8.59E-5||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ERG2(YMR202W)|FD-Score:8.38|P-value:2.60E-17||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FDC1(YDR539W)|FD-Score:6.32|P-value:1.31E-10||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:GSM1(YJL103C)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:ILV1(YER086W)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:LIN1(YHR156C)|FD-Score:4.33|P-value:7.39E-6||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MKS1(YNL076W)|FD-Score:5.07|P-value:2.04E-7||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:MTC5(YDR128W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NGG1(YDR176W)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PCL6(YER059W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PET111(YMR257C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PGM3(YMR278W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PHB2(YGR231C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PKR1(YMR123W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:SDH2(YLL041C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SKG3(YLR187W_p)|FD-Score:-4.31|P-value:8.19E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLT2(YHR030C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOL1(YNR034W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SWD3(YBR175W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TPO1(YLL028W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA2(YBR006W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YKL077W(YKL077W_p)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR413W(YLR413W_p)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YML122C(YML122C_d)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL228W(YNL228W_d)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YPR063C(YPR063C_p)|FD-Score:6.11|P-value:5.14E-10||SGD DESC:ER-localized protein of unknown function Gene:YPT6(YLR262C)|FD-Score:-5.53|P-value:1.63E-8||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YTP1(YNL237W)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:BNA4(YBL098W)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:ELA1(YNL230C)|FD-Score:-3.76|P-value:8.59E-5||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ERG2(YMR202W)|FD-Score:8.38|P-value:2.60E-17||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FDC1(YDR539W)|FD-Score:6.32|P-value:1.31E-10||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:GSM1(YJL103C)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:ILV1(YER086W)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:LIN1(YHR156C)|FD-Score:4.33|P-value:7.39E-6||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MKS1(YNL076W)|FD-Score:5.07|P-value:2.04E-7||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:MTC5(YDR128W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NGG1(YDR176W)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PCL6(YER059W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PET111(YMR257C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PGM3(YMR278W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PHB2(YGR231C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PKR1(YMR123W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:SDH2(YLL041C)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SKG3(YLR187W_p)|FD-Score:-4.31|P-value:8.19E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLT2(YHR030C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOL1(YNR034W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SWD3(YBR175W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TPO1(YLL028W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA2(YBR006W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YKL077W(YKL077W_p)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR413W(YLR413W_p)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YML122C(YML122C_d)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL228W(YNL228W_d)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YPR063C(YPR063C_p)|FD-Score:6.11|P-value:5.14E-10||SGD DESC:ER-localized protein of unknown function Gene:YPT6(YLR262C)|FD-Score:-5.53|P-value:1.63E-8||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YTP1(YNL237W)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR145W3.651.34E-40.37TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YDR454C3.611.54E-40.37GUK1Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
YNL172W3.246.02E-40.17APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YNL038W3.060.001090.07GPI15Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
YNL263C2.990.001390.08YIF1Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
YKL210W2.910.001800.10UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YML046W2.810.002450.07PRP39U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
YLR009W2.750.003010.02RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YER125W2.720.003240.02RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
YNL002C2.700.003480.08RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YOR146W_d2.610.004460.01YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YBR089W_d2.600.004630.02YBR089W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
YDR407C2.590.004849.59E-4TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YFR027W2.590.004860.09ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YKL042W2.490.006360.00SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W8.382.60E-17ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YDR539W6.321.31E-10FDC1Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm
YPR063C_p6.115.14E-10YPR063C_pER-localized protein of unknown function
YNL076W5.072.04E-7MKS1Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling
YER086W4.386.05E-6ILV1Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
YHR156C4.337.39E-6LIN1Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
YMR278W4.141.73E-5PGM3Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
YDR176W4.042.63E-5NGG1Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
YML122C_d3.865.71E-5YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLL028W3.836.36E-5TPO1Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane
YBR006W3.541.99E-4UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YNR034W3.423.15E-4SOL1Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YLR413W_p3.354.09E-4YLR413W_pPutative protein of unknown function; YLR413W is not an essential gene
YER059W3.354.10E-4PCL6Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding
YHR030C3.314.67E-4SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway

GO enrichment analysis for SGTC_3177
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2155.96E-63SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0677966ERG2
0.2041.63E-56SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.206349ERG2
0.2011.08E-54SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.155844ERG2
0.1796.46E-44SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.147541ERG2
0.1771.27E-42SGTC_2724maprotiline 69.6 μMMiscellaneous40110.130435ERG2
0.1771.45E-42SGTC_500h-89 112.0 μMICCB bioactive library4492410.0804598cell wall signaling
0.1701.65E-39SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.216216ERG2
0.1632.03E-36SGTC_1614st002383 48.6 μMTimTec (Natural product derivative library)19472350.156627cell wall signaling
0.1624.13E-36SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.205479ERG2
0.1597.91E-35SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0927835cell wall signaling
0.1591.07E-34SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.134146ERG2
0.1553.32E-33SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.131579ERG2
0.1554.56E-33SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.158537ERG2
0.1541.02E-32SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.169231ERG2
0.1524.99E-32SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.152778ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3178910750649.47 μM18897194Chembridge (Drug-like library)314.809263.76512
SGTC_3179910780149.47 μM0.764818259Chembridge (Drug-like library)318.7731433.48413
SGTC_2835900658871.43 μM0.7083332345538Chembridge (Drug-like library)300.782683.27912
SGTC_2994905352071.43 μM0.644647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_3284910817164.86 μM0.5762718687505Chembridge (Drug-like library)373.876463.7214
SGTC_3113912349849.47 μM0.5454558897275Chembridge (Drug-like library)308.417363.80812
SGTC_2939904457871.43 μM0.4461542653634Chembridge (Drug-like library)377.8403433.43915
SGTC_296090793895.97 μM0.437516457355Chembridge (Drug-like library)310.775962.36514
SGTC_2783773816571.43 μM0.41379317201110Chembridge (Drug-like library)289.756743.55612
SGTC_2838900268771.43 μM0.4032266457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)