9107506

N-(3-chloro-4-methylphenyl)-2-(3,4-dihydro-1H-isoquinolin-2-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3178
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 8897194
SMILES CC1=C(C=C(C=C1)NC(=O)CN2CCC3=CC=CC=C3C2)Cl
Standardized SMILES Cc1ccc(NC(=O)CN2CCc3ccccc3C2)cc1Cl
Molecular weight 314.8093
ALogP 3.77
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.58
% growth inhibition (Hom. pool) 12.07


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 8897194
Download HIP data (tab-delimited text)  (excel)
Gene:CDC11(YJR076C)|FD-Score:3.11|P-value:9.39E-4|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CUS1(YMR240C)|FD-Score:3.32|P-value:4.42E-4|Clearance:0.12||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:DIS3(YOL021C)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.14||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ENP1(YBR247C)|FD-Score:3.09|P-value:9.84E-4|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:MDN1(YLR106C)|FD-Score:-3.2|P-value:6.89E-4|Clearance:0||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NMD3(YHR170W)|FD-Score:5.22|P-value:8.88E-8|Clearance:0.56||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUS1(YDL193W)|FD-Score:5.57|P-value:1.31E-8|Clearance:0.56||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE8(YML092C)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.02||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RER2(YBR002C)|FD-Score:4.02|P-value:2.96E-5|Clearance:0.55||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLP24(YLR009W)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.06||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RLP7(YNL002C)|FD-Score:3.17|P-value:7.71E-4|Clearance:0.06||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPN6(YDL097C)|FD-Score:5.98|P-value:1.12E-9|Clearance:0.56||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPO31(YOR116C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.07||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPS2(YGL123W)|FD-Score:3.42|P-value:3.19E-4|Clearance:0.06||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRP42(YDL111C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP46(YGR095C)|FD-Score:3.2|P-value:6.78E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SLY1(YDR189W)|FD-Score:-3.85|P-value:5.79E-5|Clearance:0||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:SMD1(YGR074W)|FD-Score:-3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:UTP6(YDR449C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL152W(YDL152W_d)|FD-Score:4.66|P-value:1.57E-6|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YJR023C(YJR023C_d)|FD-Score:-3.31|P-value:4.63E-4|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:CDC11(YJR076C)|FD-Score:3.11|P-value:9.39E-4|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CUS1(YMR240C)|FD-Score:3.32|P-value:4.42E-4|Clearance:0.12||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:DIS3(YOL021C)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.14||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ENP1(YBR247C)|FD-Score:3.09|P-value:9.84E-4|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:MDN1(YLR106C)|FD-Score:-3.2|P-value:6.89E-4|Clearance:0||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NMD3(YHR170W)|FD-Score:5.22|P-value:8.88E-8|Clearance:0.56||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUS1(YDL193W)|FD-Score:5.57|P-value:1.31E-8|Clearance:0.56||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE8(YML092C)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.02||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RER2(YBR002C)|FD-Score:4.02|P-value:2.96E-5|Clearance:0.55||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLP24(YLR009W)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.06||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RLP7(YNL002C)|FD-Score:3.17|P-value:7.71E-4|Clearance:0.06||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPN6(YDL097C)|FD-Score:5.98|P-value:1.12E-9|Clearance:0.56||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPO31(YOR116C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.07||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPS2(YGL123W)|FD-Score:3.42|P-value:3.19E-4|Clearance:0.06||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRP42(YDL111C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP46(YGR095C)|FD-Score:3.2|P-value:6.78E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SLY1(YDR189W)|FD-Score:-3.85|P-value:5.79E-5|Clearance:0||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:SMD1(YGR074W)|FD-Score:-3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:UTP6(YDR449C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL152W(YDL152W_d)|FD-Score:4.66|P-value:1.57E-6|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YJR023C(YJR023C_d)|FD-Score:-3.31|P-value:4.63E-4|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 8897194
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:3.96|P-value:3.67E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIR2(YDL175C)|FD-Score:-4|P-value:3.13E-5||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALD3(YMR169C)|FD-Score:-4.21|P-value:1.26E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:ATP1(YBL099W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:AVT2(YEL064C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CBC2(YPL178W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:ERG3(YLR056W)|FD-Score:-3.46|P-value:2.65E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FET4(YMR319C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FPR2(YDR519W)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:FUS2(YMR232W)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GTR2(YGR163W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HFA1(YMR207C)|FD-Score:-4.97|P-value:3.29E-7||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HMO1(YDR174W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HOM2(YDR158W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HRQ1(YDR291W_p)|FD-Score:-4.45|P-value:4.39E-6||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IES1(YFL013C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:JNM1(YMR294W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LEM3(YNL323W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MAC1(YMR021C)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRH4(YGL064C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRPL13(YKR006C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MST1(YKL194C)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NBP2(YDR162C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NFT1(YKR103W)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NGG1(YDR176W)|FD-Score:-3.75|P-value:8.86E-5||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NMD2(YHR077C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NOP12(YOL041C)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:PEP12(YOR036W)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:-3.96|P-value:3.69E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEX34(YCL056C)|FD-Score:4|P-value:3.16E-5||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PSY1(YKL076C_d)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:PTK1(YKL198C)|FD-Score:-3.96|P-value:3.79E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RAD18(YCR066W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RCE1(YMR274C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RNH70(YGR276C)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPL11B(YGR085C)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.75|P-value:8.94E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:SEE1(YIL064W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SKI8(YGL213C)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:STO1(YMR125W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWM2(YNR004W_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TGS1(YPL157W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TPO5(YKL174C)|FD-Score:-3.81|P-value:6.81E-5||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:TRM7(YBR061C)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:VAN1(YML115C)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VID24(YBR105C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:YDR154C(YDR154C_d)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YFR035C(YFR035C_p)|FD-Score:5.52|P-value:1.70E-8||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YFT2(YDR319C_p)|FD-Score:4.9|P-value:4.80E-7||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR018C(YKR018C_p)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YKR073C(YKR073C_d)|FD-Score:-4.06|P-value:2.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR434C(YLR434C_d)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML047W-A(YML047W-A_d)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL211C(YNL211C_p)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOR019W(YOR019W)|FD-Score:-3.94|P-value:4.11E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR309C(YOR309C_d)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPL014W(YPL014W_p)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL247C(YPL247C_p)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR063C(YPR063C_p)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:ER-localized protein of unknown function Gene:YPT6(YLR262C)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC84(YHR016C)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ZIP1(YDR285W)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ACO1(YLR304C)|FD-Score:3.96|P-value:3.67E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIR2(YDL175C)|FD-Score:-4|P-value:3.13E-5||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALD3(YMR169C)|FD-Score:-4.21|P-value:1.26E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:ATP1(YBL099W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:AVT2(YEL064C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CBC2(YPL178W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:ERG3(YLR056W)|FD-Score:-3.46|P-value:2.65E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FET4(YMR319C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FPR2(YDR519W)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:FUS2(YMR232W)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GTR2(YGR163W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HFA1(YMR207C)|FD-Score:-4.97|P-value:3.29E-7||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HMO1(YDR174W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HOM2(YDR158W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HRQ1(YDR291W_p)|FD-Score:-4.45|P-value:4.39E-6||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IES1(YFL013C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:JNM1(YMR294W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LEM3(YNL323W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MAC1(YMR021C)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRH4(YGL064C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRPL13(YKR006C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MST1(YKL194C)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NBP2(YDR162C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NFT1(YKR103W)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NGG1(YDR176W)|FD-Score:-3.75|P-value:8.86E-5||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NMD2(YHR077C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NOP12(YOL041C)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:PEP12(YOR036W)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:-3.96|P-value:3.69E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEX34(YCL056C)|FD-Score:4|P-value:3.16E-5||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PSY1(YKL076C_d)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:PTK1(YKL198C)|FD-Score:-3.96|P-value:3.79E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RAD18(YCR066W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RCE1(YMR274C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RNH70(YGR276C)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPL11B(YGR085C)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.75|P-value:8.94E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:SEE1(YIL064W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SKI8(YGL213C)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:STO1(YMR125W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWM2(YNR004W_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TGS1(YPL157W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TPO5(YKL174C)|FD-Score:-3.81|P-value:6.81E-5||SGD DESC:Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles Gene:TRM7(YBR061C)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:VAN1(YML115C)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VID24(YBR105C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:YDR154C(YDR154C_d)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YFR035C(YFR035C_p)|FD-Score:5.52|P-value:1.70E-8||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YFT2(YDR319C_p)|FD-Score:4.9|P-value:4.80E-7||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR018C(YKR018C_p)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YKR073C(YKR073C_d)|FD-Score:-4.06|P-value:2.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR434C(YLR434C_d)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML047W-A(YML047W-A_d)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL211C(YNL211C_p)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOR019W(YOR019W)|FD-Score:-3.94|P-value:4.11E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR309C(YOR309C_d)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPL014W(YPL014W_p)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL247C(YPL247C_p)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR063C(YPR063C_p)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:ER-localized protein of unknown function Gene:YPT6(YLR262C)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC84(YHR016C)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ZIP1(YDR285W)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YDL097C5.981.12E-90.56RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YDL193W5.571.31E-80.56NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YHR170W5.228.88E-80.56NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDL152W_d4.661.57E-60.43YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YHL015W4.231.15E-50.01RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YOR116C4.231.19E-50.07RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YOL021C4.161.61E-50.14DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YBR002C4.022.96E-50.55RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YDL111C3.462.68E-40.03RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YML092C3.432.98E-40.02PRE8Alpha 2 subunit of the 20S proteasome
YGL123W3.423.19E-40.06RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YDR449C3.363.92E-40.03UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YMR240C3.324.42E-40.12CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YGR095C3.206.78E-40.04RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YNL002C3.177.71E-40.06RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YFR035C_p5.521.70E-8YFR035C_pPutative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein
YOL041C5.101.66E-7NOP12Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
YDR319C_p4.904.80E-7YFT2_pProtein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
YLR262C4.731.15E-6YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YMR232W4.552.72E-6FUS2Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
YDR158W4.532.98E-6HOM2Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YGL213C4.513.21E-6SKI8Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype
YGR163W4.444.51E-6GTR2Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
YOR036W4.356.94E-6PEP12Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
YHR077C4.161.59E-5NMD2Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance
YGR085C4.141.76E-5RPL11BRibosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication
YMR274C4.131.78E-5RCE1Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone
YCL056C4.003.16E-5PEX34Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation
YLR304C3.963.67E-5ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YMR319C3.865.62E-5FET4Low-affinity Fe(II) transporter of the plasma membrane

GO enrichment analysis for SGTC_3178
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0952.27E-13SGTC_10775-fluorocytosine 377.0 nMNIH Clinical Collection33660.047619exosome
0.0938.39E-13SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.097561RNA pol III & RNase P/MRP
0.0881.58E-11SGTC_7323970-1727 52.7 μMChemDiv (Drug-like library)12756990.102273
0.0864.51E-11SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.132353RNA pol III & RNase P/MRP
0.0856.95E-11SGTC_6781218-2179 85.9 μMChemDiv (Drug-like library)68200810.0909091RNA pol III & RNase P/MRP
0.0841.31E-10SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.111111translation
0.0831.99E-10SGTC_32529135871 49.5 μMChembridge (Drug-like library)176828380.152778
0.0814.63E-10SGTC_1310141-0277 33.8 μMChemDiv (Drug-like library)15477710.28125
0.0807.79E-10SGTC_748act1.228 77.9 μMChemDiv (Drug-like library)44215050.0983607redox potentiating
0.0808.00E-10SGTC_2513baicalein 25.6 μMMicrosource (Natural product library)52816050.1calcium & mitochondrial duress
0.0791.02E-9SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.047619exosome
0.0773.28E-9SGTC_712r015-0009 80.6 μMChemDiv (Drug-like library)68138720.0869565
0.0773.83E-9SGTC_5961155-0152 116.0 μMChemDiv (Drug-like library)2803130.107692TRP & mitochondrial translation
0.0765.31E-9SGTC_1074carmofur 3.8 μMNIH Clinical Collection25770.123288exosome
0.0741.03E-8SGTC_6173770-0098 35.0 μMChemDiv (Drug-like library)59626390.139241exosome

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3177910750649.47 μM18897194Chembridge (Drug-like library)314.809263.76512ERG2
SGTC_3179910780149.47 μM0.764818259Chembridge (Drug-like library)318.7731433.48413
SGTC_2835900658871.43 μM0.7083332345538Chembridge (Drug-like library)300.782683.27912
SGTC_2994905352071.43 μM0.644647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_3284910817164.86 μM0.5762718687505Chembridge (Drug-like library)373.876463.7214
SGTC_3113912349849.47 μM0.5454558897275Chembridge (Drug-like library)308.417363.80812
SGTC_2939904457871.43 μM0.4461542653634Chembridge (Drug-like library)377.8403433.43915
SGTC_296090793895.97 μM0.437516457355Chembridge (Drug-like library)310.775962.36514
SGTC_2783773816571.43 μM0.41379317201110Chembridge (Drug-like library)289.756743.55612
SGTC_2838900268771.43 μM0.4032266457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)