9107801

N-(3-chloro-4-fluorophenyl)-2-(3,4-dihydro-1H-isoquinolin-2-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3179
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 4818259
SMILES C1CN(CC2=CC=CC=C21)CC(=O)NC3=CC(=C(C=C3)F)Cl
Standardized SMILES Fc1ccc(NC(=O)CN2CCc3ccccc3C2)cc1Cl
Molecular weight 318.7731
ALogP 3.48
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.17
% growth inhibition (Hom. pool) 8.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4818259
Download HIP data (tab-delimited text)  (excel)
Gene:CEF1(YMR213W)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.28||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:ERD2(YBL040C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:FOL2(YGR267C)|FD-Score:-3.19|P-value:7.00E-4|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:KRI1(YNL308C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.28||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MRD1(YPR112C)|FD-Score:-6.14|P-value:4.25E-10|Clearance:0||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NUS1(YDL193W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.28||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:SPC97(YHR172W)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.28||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:CEF1(YMR213W)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.28||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:ERD2(YBL040C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:FOL2(YGR267C)|FD-Score:-3.19|P-value:7.00E-4|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:KRI1(YNL308C)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.28||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MRD1(YPR112C)|FD-Score:-6.14|P-value:4.25E-10|Clearance:0||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NUS1(YDL193W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.28||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:SPC97(YHR172W)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.28||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4818259
Download HOP data (tab-delimited text)  (excel)
Gene:ALG6(YOR002W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARL1(YBR164C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASF2(YDL197C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Anti-silencing protein that causes derepression of silent loci when overexpressed Gene:AYT1(YLL063C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis Gene:BCK2(YER167W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BOR1(YNL275W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:CAM1(YPL048W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:COX17(YLL009C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CTF18(YMR078C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:DAL7(YIR031C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:ELP4(YPL101W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ENV9(YOR246C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FAR7(YFR008W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FIR1(YER032W)|FD-Score:6.88|P-value:2.97E-12||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:ISR1(YPR106W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:MAD2(YJL030W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MAE1(YKL029C)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MKK1(YOR231W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MUM2(YBR057C)|FD-Score:4.28|P-value:9.17E-6||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NHP6A(YPR052C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NUP133(YKR082W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:PRR2(YDL214C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PYC1(YGL062W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RBL2(YOR265W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:RCE1(YMR274C)|FD-Score:4.5|P-value:3.45E-6||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RFX1(YLR176C)|FD-Score:4.32|P-value:7.97E-6||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM1(YCR028C-A)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RMA1(YKL132C)|FD-Score:-4.02|P-value:2.85E-5||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL13A(YDL082W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPS0A(YGR214W)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS23A(YGR118W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SDT1(YGL224C)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SFB3(YHR098C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SKG3(YLR187W_p)|FD-Score:-3.95|P-value:3.83E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SKI8(YGL213C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SPO22(YIL073C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SRL1(YOR247W)|FD-Score:-3.81|P-value:6.94E-5||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SRP40(YKR092C)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SWR1(YDR334W)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYH1(YPL105C)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TEL1(YBL088C)|FD-Score:-4.78|P-value:8.77E-7||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TRE1(YPL176C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UMP1(YBR173C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:VMA9(YCL005W-A)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS74(YDR372C)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAP3(YHL009C)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YCL012C(YCL012C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCR023C(YCR023C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YER137C(YER137C_p)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Putative protein of unknown function Gene:YFR020W(YFR020W_p)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR151C(YGR151C_d)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YJL045W(YJL045W)|FD-Score:-4.33|P-value:7.57E-6||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YJL215C(YJL215C_d)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR224W(YLR224W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YPR078C(YPR078C_p)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:ZEO1(YOL109W)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:ALG6(YOR002W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARL1(YBR164C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASF2(YDL197C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Anti-silencing protein that causes derepression of silent loci when overexpressed Gene:AYT1(YLL063C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis Gene:BCK2(YER167W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BOR1(YNL275W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:CAM1(YPL048W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:COX17(YLL009C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CTF18(YMR078C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:DAL7(YIR031C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:ELP4(YPL101W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ENV9(YOR246C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FAR7(YFR008W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FIR1(YER032W)|FD-Score:6.88|P-value:2.97E-12||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:ISR1(YPR106W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:MAD2(YJL030W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MAE1(YKL029C)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MKK1(YOR231W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MUM2(YBR057C)|FD-Score:4.28|P-value:9.17E-6||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NHP6A(YPR052C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NUP133(YKR082W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:PRR2(YDL214C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PYC1(YGL062W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RBL2(YOR265W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:RCE1(YMR274C)|FD-Score:4.5|P-value:3.45E-6||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RFX1(YLR176C)|FD-Score:4.32|P-value:7.97E-6||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM1(YCR028C-A)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RMA1(YKL132C)|FD-Score:-4.02|P-value:2.85E-5||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL13A(YDL082W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPS0A(YGR214W)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS23A(YGR118W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SDT1(YGL224C)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SFB3(YHR098C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SKG3(YLR187W_p)|FD-Score:-3.95|P-value:3.83E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SKI8(YGL213C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SPO22(YIL073C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SRL1(YOR247W)|FD-Score:-3.81|P-value:6.94E-5||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SRP40(YKR092C)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SWR1(YDR334W)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYH1(YPL105C)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TEL1(YBL088C)|FD-Score:-4.78|P-value:8.77E-7||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TRE1(YPL176C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UMP1(YBR173C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:VMA9(YCL005W-A)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS74(YDR372C)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YAP3(YHL009C)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YCL012C(YCL012C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCR023C(YCR023C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YER137C(YER137C_p)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Putative protein of unknown function Gene:YFR020W(YFR020W_p)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR151C(YGR151C_d)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YJL045W(YJL045W)|FD-Score:-4.33|P-value:7.57E-6||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YJL215C(YJL215C_d)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR224W(YLR224W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YPR078C(YPR078C_p)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:ZEO1(YOL109W)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL308C3.551.94E-40.28KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YDL193W3.502.35E-40.28NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YMR213W3.383.68E-40.28CEF1Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
YHR172W3.304.91E-40.28SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YNL161W3.010.001290.02CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YMR013C2.990.001380.00SEC59Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
YPL160W2.990.001400.06CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YDR373W2.930.001710.12FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YCL052C2.810.002480.04PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YDL147W2.770.002840.02RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YLR163C2.740.003030.00MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YIL150C2.740.003068.40E-4MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YDR362C2.740.003070.02TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YMR281W2.720.003250.01GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YHR196W2.710.003406.37E-4UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER032W6.882.97E-12FIR1Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate
YMR274C4.503.45E-6RCE1Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone
YLR176C4.327.97E-6RFX1Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins
YBR057C4.289.17E-6MUM2Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex
YHR098C4.211.28E-5SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YJL215C_d4.131.80E-5YJL215C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLL009C4.082.22E-5COX17Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
YER137C_p4.022.85E-5YER137C_pPutative protein of unknown function
YKL029C4.013.02E-5MAE1Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
YGL224C3.865.70E-5SDT1Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives
YPL105C3.778.05E-5SYH1Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
YOR002W3.711.05E-4ALG6Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
YLR224W_p3.711.05E-4YLR224W_pF-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene
YBR164C3.681.16E-4ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
YCR023C3.651.33E-4YCR023CVacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene

GO enrichment analysis for SGTC_3179
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0757.07E-9SGTC_9831491-0821 12.2 μMChemDiv (Drug-like library)464951130.173913
0.0722.66E-8SGTC_31599100096 49.5 μMChembridge (Drug-like library)252368750.157143
0.0649.29E-7SGTC_6941315-0438 43.2 μMChemDiv (Drug-like library)67931540.102273
0.0621.96E-6SGTC_1032k061-1018 98.7 μMChemDiv (Drug-like library)40053930.208333
0.0595.16E-6SGTC_32929120825 59.8 μMChembridge (Drug-like library)248249000.121622
0.0571.32E-5SGTC_1091telmisartan 47.6 μMMiscellaneous659990.103093
0.0552.44E-5SGTC_3061glucosamine 200.0 μMMiscellaneous914310.031746
0.0501.36E-4SGTC_1767st044992 70.3 μMTimTec (Natural product derivative library)96006930.0864198
0.0491.59E-4SGTC_31999111318 49.5 μMChembridge (Drug-like library)85480200.369231
0.0472.78E-4SGTC_2594a-mangostin 100.0 μMMicrosource (Natural product library)459342010.0759494
0.0464.16E-4SGTC_5321548-0774 215.0 μMChemDiv (Drug-like library)59329930.142857
0.0455.36E-4SGTC_512prima-1 270.0 μMICCB bioactive library3229680.0967742DNA intercalators
0.0447.45E-4SGTC_32529135871 49.5 μMChembridge (Drug-like library)176828380.136986
0.0420.00111SGTC_1846st056244 14.8 μMTimTec (Natural product derivative library)53931820.106667
0.0420.00138SGTC_14813346-2004 36.0 μMChemDiv (Drug-like library)27705220.154762

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3177910750649.47 μM0.768897194Chembridge (Drug-like library)314.809263.76512ERG2
SGTC_3178910750649.47 μM0.768897194Chembridge (Drug-like library)314.809263.76512
SGTC_2835900658871.43 μM0.7083332345538Chembridge (Drug-like library)300.782683.27912
SGTC_2994905352071.43 μM0.644647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_2939904457871.43 μM0.5666672653634Chembridge (Drug-like library)377.8403433.43915
SGTC_3113912349849.47 μM0.5178578897275Chembridge (Drug-like library)308.417363.80812
SGTC_294790726397.68 μM0.43076916488146Chembridge (Drug-like library)314.7398432.08415tubulin folding & SWR complex
SGTC_3284910817164.86 μM0.4307698687505Chembridge (Drug-like library)373.876463.7214
SGTC_2993905425771.43 μM0.3965526470217Chembridge (Drug-like library)343.850483.16113fatty acid desaturase (OLE1)
SGTC_2896905265671.43 μM0.3906256469436Chembridge (Drug-like library)369.887763.62913endomembrane recycling
SGTC_2880904298358.44 μM0.3809526464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)