9110362

1-(3-methoxyphenyl)-5-methyl-N-naphthalen-1-yltriazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3192
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 16649480
SMILES CC1=C(N=NN1C2=CC(=CC=C2)OC)C(=O)NC3=CC=CC4=CC=CC=C43
Standardized SMILES COc1cccc(c1)n2nnc(C(=O)Nc3cccc4ccccc34)c2C
Molecular weight 358.3932
ALogP 3.84
H-bond donor count 1
H-bond acceptor count 4
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.6
% growth inhibition (Hom. pool) 5.09


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16649480
Download HIP data (tab-delimited text)  (excel)
Gene:APC11(YDL008W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.07||SGD DESC:Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity Gene:CDC20(YGL116W)|FD-Score:-4.54|P-value:2.77E-6|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC3(YLR314C)|FD-Score:3.79|P-value:7.63E-5|Clearance:0.5||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC31(YOR257W)|FD-Score:3.97|P-value:3.58E-5|Clearance:0.06||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CIA2(YHR122W)|FD-Score:3.92|P-value:4.52E-5|Clearance:0.05||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:KRE9(YJL174W)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:LCP5(YER127W)|FD-Score:6.08|P-value:6.14E-10|Clearance:1.11||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MTR10(YOR160W)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.04||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR4(YJL050W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.17||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:PCF11(YDR228C)|FD-Score:-3.81|P-value:6.90E-5|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POL5(YEL055C)|FD-Score:3.13|P-value:8.84E-4|Clearance:0||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:RPB5(YBR154C)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL33A(YPL143W)|FD-Score:3.86|P-value:5.59E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPO31(YOR116C)|FD-Score:4.97|P-value:3.42E-7|Clearance:0.18||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SED5(YLR026C)|FD-Score:3.29|P-value:5.07E-4|Clearance:0.02||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:TIM54(YJL054W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.06||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TRS31(YDR472W)|FD-Score:-3.36|P-value:3.88E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TTI1(YKL033W)|FD-Score:4.79|P-value:8.35E-7|Clearance:0.65||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:UTP9(YHR196W)|FD-Score:-3.84|P-value:6.04E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YEF3(YLR249W)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YTM1(YOR272W)|FD-Score:3.86|P-value:5.62E-5|Clearance:0.08||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:APC11(YDL008W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.07||SGD DESC:Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity Gene:CDC20(YGL116W)|FD-Score:-4.54|P-value:2.77E-6|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC3(YLR314C)|FD-Score:3.79|P-value:7.63E-5|Clearance:0.5||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC31(YOR257W)|FD-Score:3.97|P-value:3.58E-5|Clearance:0.06||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CIA2(YHR122W)|FD-Score:3.92|P-value:4.52E-5|Clearance:0.05||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:KRE9(YJL174W)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:LCP5(YER127W)|FD-Score:6.08|P-value:6.14E-10|Clearance:1.11||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MTR10(YOR160W)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.04||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR4(YJL050W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.17||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:PCF11(YDR228C)|FD-Score:-3.81|P-value:6.90E-5|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POL5(YEL055C)|FD-Score:3.13|P-value:8.84E-4|Clearance:0||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:RPB5(YBR154C)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL33A(YPL143W)|FD-Score:3.86|P-value:5.59E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPO31(YOR116C)|FD-Score:4.97|P-value:3.42E-7|Clearance:0.18||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SED5(YLR026C)|FD-Score:3.29|P-value:5.07E-4|Clearance:0.02||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:TIM54(YJL054W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.06||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TRS31(YDR472W)|FD-Score:-3.36|P-value:3.88E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TTI1(YKL033W)|FD-Score:4.79|P-value:8.35E-7|Clearance:0.65||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:UTP9(YHR196W)|FD-Score:-3.84|P-value:6.04E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YEF3(YLR249W)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YTM1(YOR272W)|FD-Score:3.86|P-value:5.62E-5|Clearance:0.08||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16649480
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:API2(YDR525W_d)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APJ1(YNL077W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARP5(YNL059C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATH1(YPR026W)|FD-Score:5.31|P-value:5.49E-8||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:CAT2(YML042W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CBS1(YDL069C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CWC15(YDR163W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DET1(YDR051C)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:ENB1(YOL158C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:FIG2(YCR089W)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FKH1(YIL131C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FRT1(YOR324C)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:FRT2(YAL028W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GSC2(YGR032W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:HIS7(YBR248C)|FD-Score:-5.19|P-value:1.03E-7||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:ICE2(YIL090W)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IST2(YBR086C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:KIP1(YBL063W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:LSM7(YNL147W)|FD-Score:-3.09|P-value:0.00100||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDM34(YGL219C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP13(YGR084C)|FD-Score:-4.3|P-value:8.36E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS9(YBR146W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS51(YLR203C)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MTM1(YGR257C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:PES4(YFR023W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET117(YER058W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PNS1(YOR161C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:POP2(YNR052C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PSD2(YGR170W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:PUF6(YDR496C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RFM1(YOR279C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPB9(YGL070C)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL37B(YDR500C)|FD-Score:-3.82|P-value:6.63E-5||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:5.05|P-value:2.23E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SET3(YKR029C)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SOD2(YHR008C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPC1(YJR010C-A)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPE3(YPR069C)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:TDA5(YLR426W_p)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TPK3(YKL166C)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication Gene:TPO1(YLL028W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:YBL044W(YBL044W_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YDL176W(YDL176W)|FD-Score:-4.95|P-value:3.73E-7||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR029W(YDR029W_d)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR269C(YDR269C_d)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL138C(YGL138C_p)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGR226C(YGR226C_d)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YKL023W(YKL023W_p)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL075C(YKL075C_p)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKL077W(YKL077W_p)|FD-Score:-3.1|P-value:9.63E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR125W(YLR125W_p)|FD-Score:4.37|P-value:6.14E-6||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR278C(YLR278C_p)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YLR280C(YLR280C_d)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR1(YJR110W)|FD-Score:4.4|P-value:5.40E-6||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YOR012W(YOR012W_p)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Putative protein of unknown function Gene:YOR024W(YOR024W_d)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL264C(YPL264C_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPR077C(YPR077C_d)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:ACE2(YLR131C)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:API2(YDR525W_d)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APJ1(YNL077W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARP5(YNL059C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATH1(YPR026W)|FD-Score:5.31|P-value:5.49E-8||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:CAT2(YML042W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CBS1(YDL069C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CWC15(YDR163W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DET1(YDR051C)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:ENB1(YOL158C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:FIG2(YCR089W)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FKH1(YIL131C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FRT1(YOR324C)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:FRT2(YAL028W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GSC2(YGR032W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:HIS7(YBR248C)|FD-Score:-5.19|P-value:1.03E-7||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:ICE2(YIL090W)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IST2(YBR086C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:KIP1(YBL063W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:LSM7(YNL147W)|FD-Score:-3.09|P-value:0.00100||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDM34(YGL219C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP13(YGR084C)|FD-Score:-4.3|P-value:8.36E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS9(YBR146W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS51(YLR203C)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MTM1(YGR257C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:PES4(YFR023W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET117(YER058W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PNS1(YOR161C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:POP2(YNR052C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PSD2(YGR170W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:PUF6(YDR496C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RFM1(YOR279C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPB9(YGL070C)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL37B(YDR500C)|FD-Score:-3.82|P-value:6.63E-5||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:5.05|P-value:2.23E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SET3(YKR029C)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SOD2(YHR008C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPC1(YJR010C-A)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPE3(YPR069C)|FD-Score:4.48|P-value:3.67E-6||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:TDA5(YLR426W_p)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TPK3(YKL166C)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication Gene:TPO1(YLL028W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:YBL044W(YBL044W_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YDL176W(YDL176W)|FD-Score:-4.95|P-value:3.73E-7||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR029W(YDR029W_d)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR269C(YDR269C_d)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL138C(YGL138C_p)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGR226C(YGR226C_d)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YKL023W(YKL023W_p)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL075C(YKL075C_p)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKL077W(YKL077W_p)|FD-Score:-3.1|P-value:9.63E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR125W(YLR125W_p)|FD-Score:4.37|P-value:6.14E-6||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR278C(YLR278C_p)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YLR280C(YLR280C_d)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR1(YJR110W)|FD-Score:4.4|P-value:5.40E-6||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YOR012W(YOR012W_p)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Putative protein of unknown function Gene:YOR024W(YOR024W_d)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL264C(YPL264C_p)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPR077C(YPR077C_d)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER127W6.086.14E-101.11LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YOR116C4.973.42E-70.18RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YKL033W4.798.35E-70.65TTI1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
YJL050W4.141.76E-50.17MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YOR257W3.973.58E-50.06CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YHR122W3.924.52E-50.05CIA2Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
YPL143W3.865.59E-50.00RPL33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
YOR272W3.865.62E-50.08YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YLR314C3.797.63E-50.50CDC3Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YLR026C3.295.07E-40.02SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YJL054W3.275.47E-40.06TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
YBR154C3.216.68E-40.04RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YOR160W3.167.77E-40.04MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YEL055C3.138.84E-47.10E-4POL5DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
YDL008W3.138.86E-40.07APC11Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR026W5.315.49E-8ATH1Acid trehalase required for utilization of extracellular trehalose
YOL121C5.052.23E-7RPS19AProtein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
YPR069C4.483.67E-6SPE3Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
YJR110W4.405.40E-6YMR1Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family
YIL131C4.376.09E-6FKH1Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication
YLR125W_p4.376.14E-6YLR125W_pPutative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene
YIL090W4.337.34E-6ICE2Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
YDR051C4.337.43E-6DET1Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
YJR010C-A4.261.02E-5SPC1Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress
YBR086C4.111.99E-5IST2Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process
YOR024W_d4.022.89E-5YOR024W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL070C4.013.05E-5RPB9RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription
YKL023W_p3.963.70E-5YKL023W_pPutative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YBR146W3.661.28E-4MRPS9Mitochondrial ribosomal protein of the small subunit
YBL044W_p3.621.47E-4YBL044W_pPutative protein of unknown function; YBL044W is not an essential protein

GO enrichment analysis for SGTC_3192
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1623.65E-36SGTC_31969111891 49.5 μMChembridge (Drug-like library)188915100.168539mitochondrial processes
0.1024.84E-15SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.135135RNA pol III & RNase P/MRP
0.0832.10E-10SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.121212DNA damage response
0.0782.19E-9SGTC_6781218-2179 85.9 μMChemDiv (Drug-like library)68200810.141026RNA pol III & RNase P/MRP
0.0764.18E-9SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.112903RNA pol III & RNase P/MRP
0.0758.29E-9SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.0909091RNA pol III & RNase P/MRP
0.0741.03E-8SGTC_31989112019 49.5 μMChembridge (Drug-like library)455955550.1375mitochondrial processes
0.0741.32E-8SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.171429RNA pol III & RNase P/MRP
0.0705.89E-8SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.09375RNA pol III & RNase P/MRP
0.0699.44E-8SGTC_13581509-0022 2.5 μMChemDiv (Drug-like library)32584340.0512821
0.0691.03E-7SGTC_31919110352 49.5 μMChembridge (Drug-like library)171224240.242857
0.0656.65E-7SGTC_31939111931 49.5 μMChembridge (Drug-like library)170872440.178082
0.0589.80E-6SGTC_11770983-0204 23.6 μMChemDiv (Drug-like library)7668180.211268
0.0561.50E-5SGTC_28979048598 13.0 μMChembridge (Drug-like library)64674040.205479
0.0552.54E-5SGTC_2522biochanin a 4.9 μMMicrosource (Natural product library)52803730.144737RNA pol III & RNase P/MRP

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3168910341949.47 μM0.72222216649256Chembridge (Drug-like library)308.334542.9311460S ribosome export
SGTC_3065911388849.47 μM0.69090916648368Chembridge (Drug-like library)338.360522.91515
SGTC_3209911361649.47 μM0.64406816649353Chembridge (Drug-like library)342.77963.59514
SGTC_3079911751249.47 μM0.524597185044Chembridge (Drug-like library)338.360522.91515
SGTC_3228913179949.47 μM0.4761917015818Chembridge (Drug-like library)307.349783.15224amide catabolism
SGTC_3201911399949.47 μM0.46031716649224Chembridge (Drug-like library)336.38773.90314
SGTC_3263913701849.47 μM0.44776143844957Chembridge (Drug-like library)336.38773.20624
SGTC_3274913829849.47 μM0.44262317015756Chembridge (Drug-like library)293.32322.66624
SGTC_3325913876055.18 μM0.43478343844959Chembridge (Drug-like library)356.806183.38524Golgi
SGTC_3225913081949.47 μM0.4218754547121Chembridge (Drug-like library)293.32322.66624amide catabolism