9113082

N-(2,5-dimethoxyphenyl)-4-methoxy-3-methylbenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3195
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17018038
SMILES CC1=C(C=CC(=C1)C(=O)NC2=C(C=CC(=C2)OC)OC)OC
Standardized SMILES COc1ccc(OC)c(NC(=O)c2ccc(OC)c(C)c2)c1
Molecular weight 301.3371
ALogP 3.05
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.44
% growth inhibition (Hom. pool) 8.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17018038
Download HIP data (tab-delimited text)  (excel)
Gene:DRE2(YKR071C)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:DYS1(YHR068W)|FD-Score:3.84|P-value:6.06E-5|Clearance:0.55||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:FCF2(YLR051C)|FD-Score:-3.21|P-value:6.58E-4|Clearance:0||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GPI15(YNL038W)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.43||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:LSG1(YGL099W)|FD-Score:7.44|P-value:5.17E-14|Clearance:3.59||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NIP1(YMR309C)|FD-Score:-3.74|P-value:9.18E-5|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:ORC4(YPR162C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PWP2(YCR057C)|FD-Score:-3.24|P-value:6.04E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPA43(YOR340C)|FD-Score:-3.76|P-value:8.37E-5|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPS2(YGL123W)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:TTI1(YKL033W)|FD-Score:-4.3|P-value:8.64E-6|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:UTP6(YDR449C)|FD-Score:-4.54|P-value:2.76E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:DRE2(YKR071C)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:DYS1(YHR068W)|FD-Score:3.84|P-value:6.06E-5|Clearance:0.55||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:FCF2(YLR051C)|FD-Score:-3.21|P-value:6.58E-4|Clearance:0||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GPI15(YNL038W)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.43||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:LSG1(YGL099W)|FD-Score:7.44|P-value:5.17E-14|Clearance:3.59||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NIP1(YMR309C)|FD-Score:-3.74|P-value:9.18E-5|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:ORC4(YPR162C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PWP2(YCR057C)|FD-Score:-3.24|P-value:6.04E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPA43(YOR340C)|FD-Score:-3.76|P-value:8.37E-5|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPS2(YGL123W)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:TTI1(YKL033W)|FD-Score:-4.3|P-value:8.64E-6|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:UTP6(YDR449C)|FD-Score:-4.54|P-value:2.76E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17018038
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:-3.74|P-value:9.11E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ATP22(YDR350C)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BRE5(YNR051C)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CLA4(YNL298W)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLG1(YGL215W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:CSF1(YLR087C)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CYC7(YEL039C)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAL80(YKR034W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DIE2(YGR227W)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DUG1(YFR044C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF7(YNL136W)|FD-Score:-4.17|P-value:1.55E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ENT3(YJR125C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FAA1(YOR317W)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FIS1(YIL065C)|FD-Score:5.41|P-value:3.22E-8||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FUI1(YBL042C)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GBP2(YCL011C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GEF1(YJR040W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GND2(YGR256W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GUD1(YDL238C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:GYP1(YOR070C)|FD-Score:-3.92|P-value:4.42E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HHF1(YBR009C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIS7(YBR248C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:IMP2(YMR035W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:ISR1(YPR106W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:LRE1(YCL051W)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LST7(YGR057C)|FD-Score:6.19|P-value:3.01E-10||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MDJ1(YFL016C)|FD-Score:5.51|P-value:1.75E-8||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MPH1(YIR002C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRPL33(YMR286W)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:-4.39|P-value:5.65E-6||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:NAP1(YKR048C)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NUP133(YKR082W)|FD-Score:-4.76|P-value:9.68E-7||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:NVJ1(YHR195W)|FD-Score:-3.1|P-value:9.79E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PEX2(YJL210W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PHM8(YER037W)|FD-Score:3.83|P-value:6.37E-5||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PRY2(YKR013W)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PUF4(YGL014W)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:PYK2(YOR347C)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAD10(YML095C)|FD-Score:6.4|P-value:7.62E-11||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RFM1(YOR279C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPE1(YJL121C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL29(YFR032C-A)|FD-Score:6.7|P-value:1.06E-11||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL34B(YIL052C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RTC4(YNL254C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SAC6(YDR129C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SGE1(YPR198W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLK19(YOR195W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SOL2(YCR073W-A)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SOV1(YMR066W)|FD-Score:-6.28|P-value:1.72E-10||SGD DESC:Mitochondrial protein of unknown function Gene:SPS1(YDR523C)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis Gene:URH1(YDR400W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways Gene:URM1(YIL008W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC14(YLR386W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VMA13(YPR036W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPH1(YOR270C)|FD-Score:-5.08|P-value:1.89E-7||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:WTM1(YOR230W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBR277C(YBR277C_d)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YBT1(YLL048C)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR514C(YDR514C)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YGR151C(YGR151C_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YGR204C-A(YGR204C-A_p)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YHL012W(YHL012W_p)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YIR044C(YIR044C)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJR020W(YJR020W_d)|FD-Score:4.33|P-value:7.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL151C(YKL151C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YKR051W(YKR051W_p)|FD-Score:5.22|P-value:8.98E-8||SGD DESC:Putative protein of unknown function Gene:YLR434C(YLR434C_d)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML009W-B(YML009W-B_d)|FD-Score:-6.72|P-value:8.80E-12||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR245W(YMR245W_d)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:5.84|P-value:2.58E-9||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR343C(YOR343C_d)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:-4.99|P-value:2.99E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT32(YGL210W)|FD-Score:4.39|P-value:5.71E-6||SGD DESC:Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:-3.74|P-value:9.11E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ATP22(YDR350C)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BRE5(YNR051C)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CLA4(YNL298W)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLG1(YGL215W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:CSF1(YLR087C)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CYC7(YEL039C)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAL80(YKR034W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DIE2(YGR227W)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DUG1(YFR044C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF7(YNL136W)|FD-Score:-4.17|P-value:1.55E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ENT3(YJR125C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FAA1(YOR317W)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FIS1(YIL065C)|FD-Score:5.41|P-value:3.22E-8||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FUI1(YBL042C)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GBP2(YCL011C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GEF1(YJR040W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GND2(YGR256W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GUD1(YDL238C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:GYP1(YOR070C)|FD-Score:-3.92|P-value:4.42E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HHF1(YBR009C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIS7(YBR248C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:IMP2(YMR035W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:ISR1(YPR106W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:LRE1(YCL051W)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LST7(YGR057C)|FD-Score:6.19|P-value:3.01E-10||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MDJ1(YFL016C)|FD-Score:5.51|P-value:1.75E-8||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MPH1(YIR002C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRPL33(YMR286W)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:-4.39|P-value:5.65E-6||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:NAP1(YKR048C)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NUP133(YKR082W)|FD-Score:-4.76|P-value:9.68E-7||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:NVJ1(YHR195W)|FD-Score:-3.1|P-value:9.79E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PEX2(YJL210W)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PHM8(YER037W)|FD-Score:3.83|P-value:6.37E-5||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PRY2(YKR013W)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PUF4(YGL014W)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:PYK2(YOR347C)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAD10(YML095C)|FD-Score:6.4|P-value:7.62E-11||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RFM1(YOR279C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPE1(YJL121C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL29(YFR032C-A)|FD-Score:6.7|P-value:1.06E-11||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL34B(YIL052C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RTC4(YNL254C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SAC6(YDR129C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SGE1(YPR198W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLK19(YOR195W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SOL2(YCR073W-A)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SOV1(YMR066W)|FD-Score:-6.28|P-value:1.72E-10||SGD DESC:Mitochondrial protein of unknown function Gene:SPS1(YDR523C)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis Gene:URH1(YDR400W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways Gene:URM1(YIL008W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC14(YLR386W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VMA13(YPR036W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPH1(YOR270C)|FD-Score:-5.08|P-value:1.89E-7||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:WTM1(YOR230W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBR277C(YBR277C_d)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YBT1(YLL048C)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR514C(YDR514C)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YGR151C(YGR151C_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YGR204C-A(YGR204C-A_p)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YHL012W(YHL012W_p)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YIR044C(YIR044C)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJR020W(YJR020W_d)|FD-Score:4.33|P-value:7.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL151C(YKL151C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YKR051W(YKR051W_p)|FD-Score:5.22|P-value:8.98E-8||SGD DESC:Putative protein of unknown function Gene:YLR434C(YLR434C_d)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML009W-B(YML009W-B_d)|FD-Score:-6.72|P-value:8.80E-12||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR245W(YMR245W_d)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:5.84|P-value:2.58E-9||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR343C(YOR343C_d)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:-4.99|P-value:2.99E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT32(YGL210W)|FD-Score:4.39|P-value:5.71E-6||SGD DESC:Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W7.445.17E-143.59LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR068W3.846.06E-50.55DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YPR162C3.295.00E-40.04ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YNL038W3.255.78E-40.43GPI15Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
YHR170W2.820.002430.14NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YPR176C2.670.003750.03BET2Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YLR359W2.650.004070.08ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YNL306W2.570.005080.03MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
YLR323C2.540.005480.03CWC24Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
YGR195W2.510.005990.04SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YKL192C2.480.006660.05ACP1Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
YIL063C2.430.007600.02YRB2Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential
YDL030W2.410.008010.02PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YPL146C2.390.008360.05NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YMR013C2.350.009500.02SEC59Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFR032C-A6.701.06E-11RPL29Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
YML095C6.407.62E-11RAD10Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
YGR057C6.193.01E-10LST7Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
YNR029C_p5.842.58E-9YNR029C_pPutative protein of unknown function, deletion confers reduced fitness in saline
YFL016C5.511.75E-8MDJ1Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
YIL065C5.413.22E-8FIS1Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation
YKR051W_p5.228.98E-8YKR051W_pPutative protein of unknown function
YGR204C-A_p4.904.75E-7YGR204C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YLL048C4.415.27E-6YBT1Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
YGL210W4.395.71E-6YPT32Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
YCL051W4.376.11E-6LRE1Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication
YJR020W_d4.337.41E-6YJR020W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR523C4.279.75E-6SPS1Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis
YMR245W_d4.251.07E-5YMR245W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL151C4.151.67E-5YKL151CNADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_3195
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1574.66E-34SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.05797160S ribosome export
0.1337.93E-25SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.069444460S ribosome export
0.1271.61E-22SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.14864960S ribosome export
0.1164.11E-19SGTC_14053909-7894 12.0 μMChemDiv (Drug-like library)71040600.12121260S ribosome export
0.1151.06E-18SGTC_31909110366 49.5 μMChembridge (Drug-like library)171237680.321429
0.1125.36E-18SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.20895560S ribosome export
0.1102.13E-17SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.17721560S ribosome export
0.1093.78E-17SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.059701560S ribosome export
0.1024.74E-15SGTC_1833914-0051 30.6 μMChemDiv (Drug-like library)15962320.066666760S ribosome export
0.0961.53E-13SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.22413860S ribosome export
0.0946.01E-13SGTC_23387999974 200.0 μMChembridge (Fragment library)3159250.0769231
0.0936.29E-13SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.13235360S ribosome export
0.0936.46E-13SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.10256460S ribosome export
0.0937.28E-13SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.22413860S ribosome export
0.0912.41E-12SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.160S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3205911348349.47 μM0.67441917018033Chembridge (Drug-like library)271.311083.06813
SGTC_3203911354349.47 μM0.61904817018012Chembridge (Drug-like library)241.28513.08412
SGTC_3186911046449.47 μM0.60465117018021Chembridge (Drug-like library)269.338264.05612RPP1 & pyrimidine depletion
SGTC_3200911438549.47 μM0.57777817018030Chembridge (Drug-like library)277.2660263.49514
SGTC_3176910658249.47 μM0.55319117018027Chembridge (Drug-like library)275.730163.74812
SGTC_3164910070549.47 μM0.5217174235Chembridge (Drug-like library)299.321182.8714
SGTC_3009908081971.43 μM0.46153817027216Chembridge (Drug-like library)342.388982.43115
SGTC_8261683-5028118 μM0.458333722377ChemDiv (Drug-like library)261.703583.26212
SGTC_3067911743149.47 μM0.4489817150885Chembridge (Drug-like library)285.337663.30813
SGTC_14780453-065635.7 μM0.445258496ChemDiv (Drug-like library)347.749762.81415