9111891

N-(2-butan-2-yl-5-oxo-[1,3,4]thiadiazolo[3,2-a]pyrimidin-6-yl)-3,4-dimethoxybenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3196
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 18891510
SMILES CCC(C)C1=NN2C(=O)C(=CN=C2S1)NC(=O)C3=CC(=C(C=C3)OC)OC
Standardized SMILES CCC(C)C1=NN2C(=NC=C(NC(=O)c3ccc(OC)c(OC)c3)C2=O)S1
Molecular weight 388.4408
ALogP 2.54
H-bond donor count 1
H-bond acceptor count 7
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.16
% growth inhibition (Hom. pool) -1.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 18891510
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:3.38|P-value:3.62E-4|Clearance:0.17||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:CDC20(YGL116W)|FD-Score:-5.03|P-value:2.43E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CMD1(YBR109C)|FD-Score:4.29|P-value:8.95E-6|Clearance:0.24||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:COG4(YPR105C)|FD-Score:-4.63|P-value:1.82E-6|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ENP1(YBR247C)|FD-Score:3.21|P-value:6.65E-4|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:HYP2(YEL034W)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.02||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IPP1(YBR011C)|FD-Score:3.09|P-value:9.96E-4|Clearance:0.09||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:ISD11(YER048W-A)|FD-Score:3.17|P-value:7.50E-4|Clearance:0.06||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LCP5(YER127W)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.24||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:ORC1(YML065W)|FD-Score:-3.18|P-value:7.38E-4|Clearance:0||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:PRP4(YPR178W)|FD-Score:-4.14|P-value:1.71E-5|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB10(YOR210W)|FD-Score:3.12|P-value:9.14E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPO31(YOR116C)|FD-Score:3.93|P-value:4.19E-5|Clearance:0.24||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RSC58(YLR033W)|FD-Score:3.97|P-value:3.56E-5|Clearance:0.24||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:3.91|P-value:4.68E-5|Clearance:0.24||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC26(YDR238C)|FD-Score:3.76|P-value:8.36E-5|Clearance:0.24||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SMC5(YOL034W)|FD-Score:-3.1|P-value:9.61E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:TOM40(YMR203W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.24||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TTI1(YKL033W)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.24||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:YBR089W(YBR089W_d)|FD-Score:-3.79|P-value:7.48E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:YDL152W(YDL152W_d)|FD-Score:3.21|P-value:6.65E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:ACS2(YLR153C)|FD-Score:3.38|P-value:3.62E-4|Clearance:0.17||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:CDC20(YGL116W)|FD-Score:-5.03|P-value:2.43E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CMD1(YBR109C)|FD-Score:4.29|P-value:8.95E-6|Clearance:0.24||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:COG4(YPR105C)|FD-Score:-4.63|P-value:1.82E-6|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ENP1(YBR247C)|FD-Score:3.21|P-value:6.65E-4|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:HYP2(YEL034W)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.02||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IPP1(YBR011C)|FD-Score:3.09|P-value:9.96E-4|Clearance:0.09||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:ISD11(YER048W-A)|FD-Score:3.17|P-value:7.50E-4|Clearance:0.06||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LCP5(YER127W)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.24||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:ORC1(YML065W)|FD-Score:-3.18|P-value:7.38E-4|Clearance:0||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:PRP4(YPR178W)|FD-Score:-4.14|P-value:1.71E-5|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB10(YOR210W)|FD-Score:3.12|P-value:9.14E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPO31(YOR116C)|FD-Score:3.93|P-value:4.19E-5|Clearance:0.24||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RSC58(YLR033W)|FD-Score:3.97|P-value:3.56E-5|Clearance:0.24||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:3.91|P-value:4.68E-5|Clearance:0.24||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC26(YDR238C)|FD-Score:3.76|P-value:8.36E-5|Clearance:0.24||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SMC5(YOL034W)|FD-Score:-3.1|P-value:9.61E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:TOM40(YMR203W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.24||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TTI1(YKL033W)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.24||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:YBR089W(YBR089W_d)|FD-Score:-3.79|P-value:7.48E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:YDL152W(YDL152W_d)|FD-Score:3.21|P-value:6.65E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 18891510
Download HOP data (tab-delimited text)  (excel)
Gene:ACH1(YBL015W)|FD-Score:-5.88|P-value:1.99E-9||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADY2(YCR010C)|FD-Score:6.03|P-value:7.96E-10||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ARC18(YLR370C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BMH1(YER177W)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CIN4(YMR138W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CIT2(YCR005C)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:CMP2(YML057W)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CTR9(YOL145C)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DDR2(YOL052C-A)|FD-Score:3.72|P-value:9.97E-5||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DIE2(YGR227W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DPB4(YDR121W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:DUG1(YFR044C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:DUS4(YLR405W)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:EMP46(YLR080W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FAT1(YBR041W)|FD-Score:-3.86|P-value:5.61E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FRT1(YOR324C)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GET4(YOR164C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GLO3(YER122C)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:HBN1(YCL026C-B_p)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HGH1(YGR187C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HIS7(YBR248C)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HSV2(YGR223C)|FD-Score:-3.83|P-value:6.32E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:ILV6(YCL009C)|FD-Score:4|P-value:3.15E-5||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:INO4(YOL108C)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LSB3(YFR024C-A)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MDJ1(YFL016C)|FD-Score:5.24|P-value:7.94E-8||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MET17(YLR303W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MHO1(YJR008W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MMS22(YLR320W)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MOS1(YCL057C-A)|FD-Score:4.4|P-value:5.43E-6||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRP13(YGR084C)|FD-Score:-4.78|P-value:8.59E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRP49(YKL167C)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSS18(YPR134W)|FD-Score:5.34|P-value:4.65E-8||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MSS51(YLR203C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:OPI7(YDR360W_d)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OYE3(YPL171C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PCK1(YKR097W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:RNH1(YMR234W)|FD-Score:3.74|P-value:9.16E-5||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL37B(YDR500C)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RRT13(YER066W_p)|FD-Score:-5.35|P-value:4.48E-8||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RTT107(YHR154W)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SBH1(YER087C-B)|FD-Score:5.58|P-value:1.17E-8||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SCJ1(YMR214W)|FD-Score:-5.76|P-value:4.09E-9||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SLK19(YOR195W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SPC1(YJR010C-A)|FD-Score:7.18|P-value:3.46E-13||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPO14(YKR031C)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPS19(YNL202W)|FD-Score:4.42|P-value:4.95E-6||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SSP120(YLR250W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SVL3(YPL032C)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TRE1(YPL176C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBR1(YGR184C)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VPS38(YLR360W)|FD-Score:-4.01|P-value:2.99E-5||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:WSC2(YNL283C)|FD-Score:4.4|P-value:5.44E-6||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR027C(YBR027C_d)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR029W(YDR029W_d)|FD-Score:-4.62|P-value:1.92E-6||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR109C(YDR109C_p)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Putative kinase Gene:YDR124W(YDR124W_p)|FD-Score:-3.85|P-value:5.94E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR290W(YDR290W_d)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YHL042W(YHL042W_p)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YIG1(YPL201C)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL001W(YIL001W_p)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL016W(YJL016W_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJL160C(YJL160C_p)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJL163C(YJL163C_p)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Putative protein of unknown function Gene:YLR012C(YLR012C_p)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YML009C-A(YML009C-A_d)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Putative protein of unknown function Gene:YNL120C(YNL120C_d)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YPK3(YBR028C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL080C(YPL080C_d)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:ACH1(YBL015W)|FD-Score:-5.88|P-value:1.99E-9||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADY2(YCR010C)|FD-Score:6.03|P-value:7.96E-10||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ARC18(YLR370C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BMH1(YER177W)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CIN4(YMR138W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CIT2(YCR005C)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:CMP2(YML057W)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CTR9(YOL145C)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DDR2(YOL052C-A)|FD-Score:3.72|P-value:9.97E-5||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DIE2(YGR227W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DPB4(YDR121W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:DUG1(YFR044C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:DUS4(YLR405W)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:EMP46(YLR080W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FAT1(YBR041W)|FD-Score:-3.86|P-value:5.61E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FRT1(YOR324C)|FD-Score:-4.14|P-value:1.77E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GET4(YOR164C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GLO3(YER122C)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:HBN1(YCL026C-B_p)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HGH1(YGR187C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HIS7(YBR248C)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HSV2(YGR223C)|FD-Score:-3.83|P-value:6.32E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:ILV6(YCL009C)|FD-Score:4|P-value:3.15E-5||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:INO4(YOL108C)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LSB3(YFR024C-A)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MDJ1(YFL016C)|FD-Score:5.24|P-value:7.94E-8||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MET17(YLR303W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MHO1(YJR008W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MMS22(YLR320W)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MOS1(YCL057C-A)|FD-Score:4.4|P-value:5.43E-6||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRP13(YGR084C)|FD-Score:-4.78|P-value:8.59E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRP49(YKL167C)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSS18(YPR134W)|FD-Score:5.34|P-value:4.65E-8||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MSS51(YLR203C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:OPI7(YDR360W_d)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OYE3(YPL171C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PCK1(YKR097W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:RNH1(YMR234W)|FD-Score:3.74|P-value:9.16E-5||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL37B(YDR500C)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RRT13(YER066W_p)|FD-Score:-5.35|P-value:4.48E-8||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RTT107(YHR154W)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SBH1(YER087C-B)|FD-Score:5.58|P-value:1.17E-8||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SCJ1(YMR214W)|FD-Score:-5.76|P-value:4.09E-9||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SLK19(YOR195W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SPC1(YJR010C-A)|FD-Score:7.18|P-value:3.46E-13||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPO14(YKR031C)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPS19(YNL202W)|FD-Score:4.42|P-value:4.95E-6||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SSP120(YLR250W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SVL3(YPL032C)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TRE1(YPL176C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBR1(YGR184C)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VPS38(YLR360W)|FD-Score:-4.01|P-value:2.99E-5||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:WSC2(YNL283C)|FD-Score:4.4|P-value:5.44E-6||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR027C(YBR027C_d)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR029W(YDR029W_d)|FD-Score:-4.62|P-value:1.92E-6||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR109C(YDR109C_p)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Putative kinase Gene:YDR124W(YDR124W_p)|FD-Score:-3.85|P-value:5.94E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR290W(YDR290W_d)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YHL042W(YHL042W_p)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YIG1(YPL201C)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL001W(YIL001W_p)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL016W(YJL016W_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJL160C(YJL160C_p)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJL163C(YJL163C_p)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Putative protein of unknown function Gene:YLR012C(YLR012C_p)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YML009C-A(YML009C-A_d)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Putative protein of unknown function Gene:YNL120C(YNL120C_d)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YPK3(YBR028C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL080C(YPL080C_d)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR109C4.298.95E-60.24CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YER127W4.121.89E-50.24LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YKL033W4.102.08E-50.24TTI1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
YLR033W3.973.56E-50.24RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YOR116C3.934.19E-50.24RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YML127W3.914.68E-50.24RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YDR238C3.768.36E-50.24SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YMR203W3.621.50E-40.24TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YLR153C3.383.62E-40.17ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YBR247C3.216.65E-42.01E-4ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
YDL152W_d3.216.65E-40.03YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YER048W-A3.177.50E-40.06ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YOR210W3.129.14E-40.00RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YEL034W3.119.27E-40.02HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YBR011C3.099.96E-40.09IPP1Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR010C-A7.183.46E-13SPC1Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress
YCR010C6.037.96E-10ADY2Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication
YER087C-B5.581.17E-8SBH1Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p
YPR134W5.344.65E-8MSS18Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
YFL016C5.247.94E-8MDJ1Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
YLR320W5.042.34E-7MMS22Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation
YML057W4.856.12E-7CMP2Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
YLR250W4.701.32E-6SSP120Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YKR097W4.631.81E-6PCK1Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
YML009C-A_d4.562.58E-6YML009C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR187C4.434.76E-6HGH1Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress
YNL202W4.424.95E-6SPS19Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
YCL057C-A4.405.43E-6MOS1Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1
YNL283C4.405.44E-6WSC2Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
YCL009C4.003.15E-5ILV6Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria

GO enrichment analysis for SGTC_3196
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1674.11E-38SGTC_31989112019 49.5 μMChembridge (Drug-like library)455955550.0777778mitochondrial processes
0.1623.65E-36SGTC_31929110362 49.5 μMChembridge (Drug-like library)166494800.168539mitochondrial processes
0.0953.07E-13SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.084507
0.0912.40E-12SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0561798RSC complex & mRNA processing
0.0898.19E-12SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.116279
0.0863.60E-11SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.153846RNA pol III & RNase P/MRP
0.0841.29E-10SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.0977444RNA pol III & RNase P/MRP
0.0814.99E-10SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0547945
0.0815.62E-10SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.105263heme biosynthesis & mitochondrial translocase
0.0782.37E-9SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.12
0.0782.45E-9SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0652174heme biosynthesis & mitochondrial translocase
0.0773.06E-9SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.144578
0.0773.43E-9SGTC_1114scopoletin 1.7 μMTimTec (Natural product library)52804600.148649
0.0765.16E-9SGTC_32869117135 37.2 μMChembridge (Drug-like library)34381500.139535
0.0765.60E-9SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.0526316DNA damage response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2787528454071.43 μM0.358209736958Chembridge (Drug-like library)291.729563.24613
SGTC_13001094-0077113 μM0.3205135532636ChemDiv (Drug-like library)418.241342.68515
SGTC_3203911354349.47 μM0.31343317018012Chembridge (Drug-like library)241.28513.08412
SGTC_3186911046449.47 μM0.30882417018021Chembridge (Drug-like library)269.338264.05612RPP1 & pyrimidine depletion
SGTC_3195911308249.47 μM0.30434817018038Chembridge (Drug-like library)301.337063.05114
SGTC_3200911438549.47 μM0.317018030Chembridge (Drug-like library)277.2660263.49514
SGTC_3205911348349.47 μM0.29577517018033Chembridge (Drug-like library)271.311083.06813
SGTC_3176910658249.47 μM0.29166717018027Chembridge (Drug-like library)275.730163.74812
SGTC_3014908153349.47 μM0.28571421367265Chembridge (Drug-like library)285.3128433.81113
SGTC_3094911774249.47 μM0.27631617149698Chembridge (Drug-like library)312.3632.8523