0928-0142

(5E)-5-[(3,4-dihydroxyphenyl)methylidene]-3-phenyl-2-sulfanylidene-1,3-thiazolidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_32
Screen concentration 34.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 2244242
SMILES C1=CC=C(C=C1)N2C(=O)C(=CC3=CC(=C(C=C3)O)O)SC2=S
Standardized SMILES Oc1ccc(C=C2SC(=S)N(C2=O)c3ccccc3)cc1O
Molecular weight 329.3934
ALogP 4.14
H-bond donor count 2
H-bond acceptor count 5
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 46.4
% growth inhibition (Hom. pool) 24.88


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2244242
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.05||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARC35(YNR035C)|FD-Score:5.22|P-value:9.18E-8|Clearance:0.45||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ARP4(YJL081C)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.06||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BRL1(YHR036W)|FD-Score:4.77|P-value:9.29E-7|Clearance:0.21||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:DIS3(YOL021C)|FD-Score:3.2|P-value:6.79E-4|Clearance:0.03||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:DRS1(YLL008W)|FD-Score:-3.36|P-value:3.86E-4|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG7(YHR072W)|FD-Score:4.56|P-value:2.57E-6|Clearance:0.27||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:OLE1(YGL055W)|FD-Score:7.05|P-value:9.26E-13|Clearance:1.83||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:POP6(YGR030C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPB3(YIL021W)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.09||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC53(YDL150W)|FD-Score:4.29|P-value:9.11E-6|Clearance:0.1||SGD DESC:RNA polymerase III subunit C53 Gene:RPC82(YPR190C)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.05||SGD DESC:RNA polymerase III subunit C82 Gene:RPN2(YIL075C)|FD-Score:4.09|P-value:2.13E-5|Clearance:0.61||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:SIS1(YNL007C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.1||SGD DESC:Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress Gene:YDL152W(YDL152W_d)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YJL015C(YJL015C_d)|FD-Score:-3.96|P-value:3.79E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:ACT1(YFL039C)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.05||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARC35(YNR035C)|FD-Score:5.22|P-value:9.18E-8|Clearance:0.45||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ARP4(YJL081C)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.06||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BRL1(YHR036W)|FD-Score:4.77|P-value:9.29E-7|Clearance:0.21||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:DIS3(YOL021C)|FD-Score:3.2|P-value:6.79E-4|Clearance:0.03||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:DRS1(YLL008W)|FD-Score:-3.36|P-value:3.86E-4|Clearance:0||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG7(YHR072W)|FD-Score:4.56|P-value:2.57E-6|Clearance:0.27||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:OLE1(YGL055W)|FD-Score:7.05|P-value:9.26E-13|Clearance:1.83||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:POP6(YGR030C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPB3(YIL021W)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.09||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC53(YDL150W)|FD-Score:4.29|P-value:9.11E-6|Clearance:0.1||SGD DESC:RNA polymerase III subunit C53 Gene:RPC82(YPR190C)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.05||SGD DESC:RNA polymerase III subunit C82 Gene:RPN2(YIL075C)|FD-Score:4.09|P-value:2.13E-5|Clearance:0.61||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:SIS1(YNL007C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.1||SGD DESC:Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress Gene:YDL152W(YDL152W_d)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YJL015C(YJL015C_d)|FD-Score:-3.96|P-value:3.79E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2244242
Download HOP data (tab-delimited text)  (excel)
Gene:ADD66(YKL206C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:ALO1(YML086C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:APD1(YBR151W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:CAT2(YML042W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:COX10(YPL172C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:ENV10(YLR065C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FET4(YMR319C)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FOX2(YKR009C)|FD-Score:5.66|P-value:7.41E-9||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GYP6(YJL044C)|FD-Score:5.99|P-value:1.07E-9||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HEM14(YER014W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIM1(YDR317W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Protein of unknown function involved in DNA repair Gene:HMX1(YLR205C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:MEP2(YNL142W)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:NCL1(YBL024W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NSG1(YHR133C)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OAC1(YKL120W)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:PLB2(YMR006C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PSP1(YDR505C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:PUN1(YLR414C)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:PUS2(YGL063W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RBK1(YCR036W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative ribokinase Gene:RIM11(YMR139W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SNQ2(YDR011W)|FD-Score:4.85|P-value:6.15E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:STM1(YLR150W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TDA9(YML081W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-3.8|P-value:7.21E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:23.7|P-value:6.53E-125||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBR1(YGR184C)|FD-Score:-3.82|P-value:6.77E-5||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VPS21(YOR089C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBL010C(YBL010C_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YER079W(YER079W_p)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function Gene:YLR269C(YLR269C_d)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR042W(YNR042W_d)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Gene:YOR283W(YOR283W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ADD66(YKL206C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:ALO1(YML086C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:APD1(YBR151W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:CAT2(YML042W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:COX10(YPL172C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:ENV10(YLR065C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FET4(YMR319C)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FOX2(YKR009C)|FD-Score:5.66|P-value:7.41E-9||SGD DESC:Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Gene:GYP6(YJL044C)|FD-Score:5.99|P-value:1.07E-9||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HEM14(YER014W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIM1(YDR317W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Protein of unknown function involved in DNA repair Gene:HMX1(YLR205C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:MEP2(YNL142W)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:NCL1(YBL024W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NSG1(YHR133C)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OAC1(YKL120W)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:PLB2(YMR006C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PSP1(YDR505C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:PUN1(YLR414C)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:PUS2(YGL063W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RBK1(YCR036W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative ribokinase Gene:RIM11(YMR139W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SNQ2(YDR011W)|FD-Score:4.85|P-value:6.15E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:STM1(YLR150W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TDA9(YML081W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-3.8|P-value:7.21E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:23.7|P-value:6.53E-125||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBR1(YGR184C)|FD-Score:-3.82|P-value:6.77E-5||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VPS21(YOR089C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBL010C(YBL010C_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YER079W(YER079W_p)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function Gene:YLR269C(YLR269C_d)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR042W(YNR042W_d)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Gene:YOR283W(YOR283W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL055W7.059.26E-131.83OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YNR035C5.229.18E-80.45ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YHR036W4.779.29E-70.21BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YHR072W4.562.57E-60.27ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YDL150W4.299.11E-60.10RPC53RNA polymerase III subunit C53
YIL021W4.181.43E-50.09RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YIL075C4.092.13E-50.61RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YDL152W_d3.482.51E-40.14YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YFL039C3.344.13E-40.05ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YJL081C3.294.98E-40.06ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YGR030C3.236.20E-40.03POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOL021C3.206.79E-40.03DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YPR190C3.177.52E-40.05RPC82RNA polymerase III subunit C82
YNL007C3.138.89E-40.10SIS1Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
YOR260W3.030.001230.12GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A23.706.53E-125TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YJL044C5.991.07E-9GYP6GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport
YKR009C5.667.41E-9FOX2Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
YDR011W4.856.15E-7SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YBR151W4.141.77E-5APD1Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
YBL010C_p4.131.82E-5YBL010C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
YLR414C4.032.73E-5PUN1Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress
YLR205C3.836.42E-5HMX1ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
YDR317W3.787.72E-5HIM1Protein of unknown function involved in DNA repair
YOR283W3.591.68E-4YOR283WPhosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene
YMR139W3.571.79E-4RIM11Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress
YML042W3.492.42E-4CAT2Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes
YCR036W3.442.87E-4RBK1Putative ribokinase
YKL126W3.403.42E-4YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR065C3.373.77E-4ENV10Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene

GO enrichment analysis for SGTC_32
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5520SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0606061TSC3-RPN4
0.5210SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.144737TSC3-RPN4
0.5180SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0810811TSC3-RPN4
0.4980SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.142857TSC3-RPN4
0.4880SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0833333TSC3-RPN4
0.4830SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.056338TSC3-RPN4
0.4601.42E-307SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.307692TSC3-RPN4
0.4556.53E-299SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0843373TSC3-RPN4
0.4362.44E-272SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0447761TSC3-RPN4
0.4329.02E-268SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0909091TSC3-RPN4
0.4214.60E-252SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0289855TSC3-RPN4
0.4034.98E-229SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.196721TSC3-RPN4
0.4025.41E-227SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.109375
0.3989.34E-224SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.147541TSC3-RPN4
0.3981.32E-223SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0897436

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_5203584-0023682 μM0.3508771340790ChemDiv (Drug-like library)293.361342.81505
SGTC_18835-benzylidenerhodanine20 μM0.341273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_24545-benzylidenerhodanine10.36 μM0.341273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_24305571206200 μM0.3278691731771Miscellaneous315.409924.34704
SGTC_24345670830169.91 μM0.3275861549830Miscellaneous250.33992.67404
SGTC_2429590284464.44 μM0.3272731927790Miscellaneous225.287382.44204
SGTC_2241677262537.81 μM0.3157892285411Chembridge (Fragment library)259.280463.09814
SGTC_2452586313648.41 μM0.3103452184096Miscellaneous253.340543.16804
SGTC_24625162487171.77 μM0.3103452259986Miscellaneous239.313962.79104
SGTC_23276189656200 μM0.3090915349088Chembridge (Fragment library)282.702882.18634
SGTC_1882561564320 μM0.3088242253075Miscellaneous392.8979635.84215TRP & mitochondrial translation