4534-3890

2-(4-chloro-2-methylphenoxy)-1-(4-pyridin-2-ylpiperazin-1-yl)ethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_321
Screen concentration 65.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 699746
SMILES CC1=C(C=CC(=C1)Cl)OCC(=O)N2CCN(CC2)C3=CC=CC=N3
Standardized SMILES Cc1cc(Cl)ccc1OCC(=O)N2CCN(CC2)c3ccccn3
Molecular weight 345.8233
ALogP 3.25
H-bond donor count 0
H-bond acceptor count 4
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 26.47
% growth inhibition (Hom. pool) 12.27


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 699746
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:3.11|P-value:9.50E-4|Clearance:0.03||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:5.09|P-value:1.82E-7|Clearance:0.66||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CFD1(YIL003W)|FD-Score:-3.34|P-value:4.25E-4|Clearance:0||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:CNS1(YBR155W)|FD-Score:4.09|P-value:2.15E-5|Clearance:0.25||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:DSN1(YIR010W)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.04||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:HSP60(YLR259C)|FD-Score:3.8|P-value:7.27E-5|Clearance:0.11||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:MPS1(YDL028C)|FD-Score:-3.47|P-value:2.57E-4|Clearance:0||SGD DESC:Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p Gene:PGI1(YBR196C)|FD-Score:3.84|P-value:6.10E-5|Clearance:0.04||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RLP24(YLR009W)|FD-Score:4.3|P-value:8.58E-6|Clearance:0.12||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:4.42|P-value:4.85E-6|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMC2(YFR031C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:TRL1(YJL087C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.09||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:TTI1(YKL033W)|FD-Score:-3.23|P-value:6.19E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:YGR073C(YGR073C_d)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YOR146W(YOR146W_d)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.35||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL238C(YPL238C_d)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPR136C(YPR136C_d)|FD-Score:6.15|P-value:3.76E-10|Clearance:1.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:CCT3(YJL014W)|FD-Score:3.11|P-value:9.50E-4|Clearance:0.03||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:5.09|P-value:1.82E-7|Clearance:0.66||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CFD1(YIL003W)|FD-Score:-3.34|P-value:4.25E-4|Clearance:0||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:CNS1(YBR155W)|FD-Score:4.09|P-value:2.15E-5|Clearance:0.25||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:DSN1(YIR010W)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.04||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:HSP60(YLR259C)|FD-Score:3.8|P-value:7.27E-5|Clearance:0.11||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:MPS1(YDL028C)|FD-Score:-3.47|P-value:2.57E-4|Clearance:0||SGD DESC:Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p Gene:PGI1(YBR196C)|FD-Score:3.84|P-value:6.10E-5|Clearance:0.04||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RLP24(YLR009W)|FD-Score:4.3|P-value:8.58E-6|Clearance:0.12||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:4.42|P-value:4.85E-6|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMC2(YFR031C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:TRL1(YJL087C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.09||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:TTI1(YKL033W)|FD-Score:-3.23|P-value:6.19E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:YGR073C(YGR073C_d)|FD-Score:3.54|P-value:2.03E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YOR146W(YOR146W_d)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.35||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL238C(YPL238C_d)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPR136C(YPR136C_d)|FD-Score:6.15|P-value:3.76E-10|Clearance:1.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 699746
Download HOP data (tab-delimited text)  (excel)
Gene:ADH4(YGL256W)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:CIN8(YEL061C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:ENO1(YGR254W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERT1(YBR239C_p)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:GIC1(YHR061C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MDH2(YOL126C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MTC3(YGL226W)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NOP13(YNL175C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:PGM1(YKL127W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PHD1(YKL043W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PPR1(YLR014C)|FD-Score:4.97|P-value:3.41E-7||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PPZ2(YDR436W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RAD18(YCR066W)|FD-Score:4|P-value:3.23E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RPL18B(YNL301C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL26A(YLR344W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPN4(YDL020C)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:TVP15(YDR100W)|FD-Score:-3.86|P-value:5.74E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:VPS71(YML041C)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCR015C(YCR015C_p)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YEL014C(YEL014C_d)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJL068C(YJL068C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJL136W-A(YJL136W-A_p)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YKL107W(YKL107W_p)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YKR023W(YKR023W_p)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ADH4(YGL256W)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:CIN8(YEL061C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:ENO1(YGR254W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERT1(YBR239C_p)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:GIC1(YHR061C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MDH2(YOL126C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MTC3(YGL226W)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NOP13(YNL175C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:PGM1(YKL127W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PHD1(YKL043W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PPR1(YLR014C)|FD-Score:4.97|P-value:3.41E-7||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PPZ2(YDR436W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RAD18(YCR066W)|FD-Score:4|P-value:3.23E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RPL18B(YNL301C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL26A(YLR344W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPN4(YDL020C)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:TVP15(YDR100W)|FD-Score:-3.86|P-value:5.74E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:VPS71(YML041C)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCR015C(YCR015C_p)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YEL014C(YEL014C_d)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR125W(YGR125W_p)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YJL068C(YJL068C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJL136W-A(YJL136W-A_p)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YKL107W(YKL107W_p)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YKR023W(YKR023W_p)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR136C_d6.153.76E-101.07YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YGL116W5.091.82E-70.66CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YHR062C4.424.85E-60.12RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR009W4.308.58E-60.12RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YJL087C4.181.43E-50.09TRL1tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
YBR155W4.092.15E-50.25CNS1TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
YBR196C3.846.10E-50.04PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YLR259C3.807.27E-50.11HSP60Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
YFR031C3.681.15E-40.00SMC2Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
YPL238C_d3.681.16E-40.10YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YIR010W3.581.72E-40.04DSN1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
YGR073C_d3.542.03E-40.08YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YOR146W_d3.452.77E-40.35YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YJL014W3.119.50E-40.03CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YIL063C3.080.001050.14YRB2Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR014C4.973.41E-7PPR1Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
YBR239C_p4.042.63E-5ERT1_pTranscriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition
YCR066W4.003.23E-5RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YGL226W3.924.36E-5MTC3Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
YGL256W3.787.83E-5ADH4Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
YKR023W_p3.749.28E-5YKR023W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YJL068C3.691.14E-4YJL068CNon-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
YJL136W-A_p3.651.30E-4YJL136W-A_pPutative protein of unknown function; identified by SAGE
YLR344W3.512.24E-4RPL26ARibosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
YOL126C3.492.44E-4MDH2Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1
YIL018W3.363.85E-4RPL2BRibosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures
YNL301C3.354.04E-4RPL18BRibosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication
YEL014C_d3.354.10E-4YEL014C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL127W3.314.59E-4PGM1Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism
YCR015C_p3.314.61E-4YCR015C_pPutative protein of unknown function; YCR015C is not an essential gene

GO enrichment analysis for SGTC_321
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0872.52E-11SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0941176RPP1 & pyrimidine depletion
0.0872.59E-11SGTC_2549cedrol 20.9 μMMicrosource (Natural product library)67086650.0273973
0.0831.59E-10SGTC_9164466-0038 42.0 nMChemDiv (Drug-like library)57220640.109589cell wall
0.0807.75E-10SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.189873RPP1 & pyrimidine depletion
0.0807.63E-10SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.103448RPP1 & pyrimidine depletion
0.0759.65E-9SGTC_29207982904 15.8 μMChembridge (Drug-like library)64568080.191781
0.0731.61E-8SGTC_3301488-0319 80.1 μMChemDiv (Drug-like library)68466140.0813954RPP1 & pyrimidine depletion
0.0731.82E-8SGTC_7560508-1999 28.3 μMChemDiv (Drug-like library)67538530.134146RPP1 & pyrimidine depletion
0.0691.25E-7SGTC_571k831-0106 44.2 μMChemDiv (Drug-like library)47836390.16
0.0681.50E-7SGTC_1927st060202 13.8 μMTimTec (Natural product derivative library)6888870.101449
0.0681.73E-7SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.103448RNA pol III & RNase P/MRP
0.0631.17E-6SGTC_31149122766 49.5 μMChembridge (Drug-like library)171769850.24
0.0631.20E-6SGTC_1115galangin 695.7 nMTimTec (Natural product library)52816160.0945946RPP1 & pyrimidine depletion
0.0631.50E-6SGTC_7353076-0345 636.0 μMChemDiv (Drug-like library)28889900.0987654
0.0612.31E-6SGTC_1642st009582 77.7 μMTimTec (Natural product derivative library)67393110.123288RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3020908914049.47 μM0.66071417217859Chembridge (Drug-like library)366.241783.42604
SGTC_3157909927449.47 μM0.63157917217662Chembridge (Drug-like library)339.43143.29104
SGTC_3146909560949.47 μM0.54838717173999Chembridge (Drug-like library)380.268363.9104
SGTC_2970909093841.86 μM0.53225817217104Chembridge (Drug-like library)339.43143.55304RNA processing & uracil transport
SGTC_3156909993149.47 μM0.50847518111053Chembridge (Drug-like library)344.835243.85903
SGTC_3136909567249.47 μM0.50793717217380Chembridge (Drug-like library)373.876464.21804
SGTC_3058909386149.47 μM0.49253717217477Chembridge (Drug-like library)373.876464.25504
SGTC_3028908777849.47 μM0.49230817217395Chembridge (Drug-like library)373.876464.04304
SGTC_3160910021649.47 μM0.49230817191039Chembridge (Drug-like library)359.849883.73204
SGTC_22267298446200 μM0.4666673283010Chembridge (Fragment library)287.379982.70104