9128157

6-(furan-2-yl)-3-(2-phenylethyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3210
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17590849
SMILES C1=CC=C(C=C1)CCC2=NN=C3N2N=C(S3)C4=CC=CO4
Standardized SMILES C(Cc1nnc2sc(nn12)c3occc3)c4ccccc4
Molecular weight 296.347
ALogP 3.26
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.89
% growth inhibition (Hom. pool) 15.54


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17590849
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:-4.46|P-value:4.09E-6|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:ALG7(YBR243C)|FD-Score:3.17|P-value:7.57E-4|Clearance:0.03||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:APC4(YDR118W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:BET2(YPR176C)|FD-Score:-3.32|P-value:4.42E-4|Clearance:0||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC25(YLR310C)|FD-Score:3.14|P-value:8.43E-4|Clearance:0.03||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC43(YGL155W)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.05||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:CDC53(YDL132W)|FD-Score:4.83|P-value:6.66E-7|Clearance:0.07||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CFD1(YIL003W)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:CWC23(YGL128C)|FD-Score:-5.88|P-value:2.05E-9|Clearance:0||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:CWC24(YLR323C)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DPB11(YJL090C)|FD-Score:-5.04|P-value:2.30E-7|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DSN1(YIR010W)|FD-Score:3.2|P-value:6.78E-4|Clearance:0.03||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:EFB1(YAL003W)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.15||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERO1(YML130C)|FD-Score:-3.09|P-value:9.87E-4|Clearance:0||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:IDI1(YPL117C)|FD-Score:3.14|P-value:8.38E-4|Clearance:0||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:LSM2(YBL026W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MIF2(YKL089W)|FD-Score:-3.83|P-value:6.29E-5|Clearance:0||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:NAB2(YGL122C)|FD-Score:5.61|P-value:1.03E-8|Clearance:0.5||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NFS1(YCL017C)|FD-Score:3.22|P-value:6.46E-4|Clearance:0.01||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NUP57(YGR119C)|FD-Score:-4.09|P-value:2.14E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PCF11(YDR228C)|FD-Score:4.33|P-value:7.59E-6|Clearance:0.01||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POL3(YDL102W)|FD-Score:3.82|P-value:6.67E-5|Clearance:0.22||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE10(YOR362C)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.05||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:4.32|P-value:7.90E-6|Clearance:0.18||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE6(YOL038W)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.01||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE8(YML092C)|FD-Score:4.77|P-value:9.38E-7|Clearance:0.11||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RAD53(YPL153C)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.07||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RPC31(YNL151C)|FD-Score:5.61|P-value:9.91E-9|Clearance:0.5||SGD DESC:RNA polymerase III subunit C31 Gene:RPC53(YDL150W)|FD-Score:-3.18|P-value:7.41E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPS15(YOL040C)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC58(YLR033W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SAM35(YHR083W)|FD-Score:-5.63|P-value:9.14E-9|Clearance:0||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SDO1(YLR022C)|FD-Score:-7.63|P-value:1.20E-14|Clearance:0||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC10(YLR166C)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.12||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SLU7(YDR088C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.01||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SMT3(YDR510W)|FD-Score:3.98|P-value:3.38E-5|Clearance:0.09||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPC3(YLR066W)|FD-Score:4.54|P-value:2.83E-6|Clearance:0.13||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:TAF11(YML015C)|FD-Score:-3.57|P-value:1.75E-4|Clearance:0||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:TCP1(YDR212W)|FD-Score:-3.14|P-value:8.57E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TRS20(YBR254C)|FD-Score:5.1|P-value:1.67E-7|Clearance:0.27||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:UTP8(YGR128C)|FD-Score:-4.88|P-value:5.32E-7|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDR187C(YDR187C_d)|FD-Score:-3.64|P-value:1.38E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YDR355C(YDR355C_d)|FD-Score:-5.09|P-value:1.77E-7|Clearance:0||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:YGL102C(YGL102C_d)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:YGR115C(YGR115C_d)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YKL153W(YKL153W_d)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YRB1(YDR002W)|FD-Score:4.41|P-value:5.20E-6|Clearance:0.05||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:ZIM17(YNL310C)|FD-Score:4.35|P-value:6.67E-6|Clearance:0.03||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix Gene:ALA1(YOR335C)|FD-Score:-4.46|P-value:4.09E-6|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:ALG7(YBR243C)|FD-Score:3.17|P-value:7.57E-4|Clearance:0.03||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:APC4(YDR118W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:BET2(YPR176C)|FD-Score:-3.32|P-value:4.42E-4|Clearance:0||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC25(YLR310C)|FD-Score:3.14|P-value:8.43E-4|Clearance:0.03||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC43(YGL155W)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.05||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:CDC53(YDL132W)|FD-Score:4.83|P-value:6.66E-7|Clearance:0.07||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CFD1(YIL003W)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:CWC23(YGL128C)|FD-Score:-5.88|P-value:2.05E-9|Clearance:0||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:CWC24(YLR323C)|FD-Score:-3.68|P-value:1.15E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DPB11(YJL090C)|FD-Score:-5.04|P-value:2.30E-7|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DSN1(YIR010W)|FD-Score:3.2|P-value:6.78E-4|Clearance:0.03||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:EFB1(YAL003W)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.15||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERO1(YML130C)|FD-Score:-3.09|P-value:9.87E-4|Clearance:0||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:IDI1(YPL117C)|FD-Score:3.14|P-value:8.38E-4|Clearance:0||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:LSM2(YBL026W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MIF2(YKL089W)|FD-Score:-3.83|P-value:6.29E-5|Clearance:0||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:NAB2(YGL122C)|FD-Score:5.61|P-value:1.03E-8|Clearance:0.5||SGD DESC:Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress Gene:NFS1(YCL017C)|FD-Score:3.22|P-value:6.46E-4|Clearance:0.01||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NUP57(YGR119C)|FD-Score:-4.09|P-value:2.14E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PCF11(YDR228C)|FD-Score:4.33|P-value:7.59E-6|Clearance:0.01||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:POL3(YDL102W)|FD-Score:3.82|P-value:6.67E-5|Clearance:0.22||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE10(YOR362C)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.05||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:4.32|P-value:7.90E-6|Clearance:0.18||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE6(YOL038W)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.01||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE8(YML092C)|FD-Score:4.77|P-value:9.38E-7|Clearance:0.11||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RAD53(YPL153C)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.07||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RPC31(YNL151C)|FD-Score:5.61|P-value:9.91E-9|Clearance:0.5||SGD DESC:RNA polymerase III subunit C31 Gene:RPC53(YDL150W)|FD-Score:-3.18|P-value:7.41E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPS15(YOL040C)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC58(YLR033W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SAM35(YHR083W)|FD-Score:-5.63|P-value:9.14E-9|Clearance:0||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SDO1(YLR022C)|FD-Score:-7.63|P-value:1.20E-14|Clearance:0||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC10(YLR166C)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.12||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SLU7(YDR088C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.01||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SMT3(YDR510W)|FD-Score:3.98|P-value:3.38E-5|Clearance:0.09||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPC3(YLR066W)|FD-Score:4.54|P-value:2.83E-6|Clearance:0.13||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:TAF11(YML015C)|FD-Score:-3.57|P-value:1.75E-4|Clearance:0||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:TCP1(YDR212W)|FD-Score:-3.14|P-value:8.57E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TRS20(YBR254C)|FD-Score:5.1|P-value:1.67E-7|Clearance:0.27||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:UTP8(YGR128C)|FD-Score:-4.88|P-value:5.32E-7|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDR187C(YDR187C_d)|FD-Score:-3.64|P-value:1.38E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YDR355C(YDR355C_d)|FD-Score:-5.09|P-value:1.77E-7|Clearance:0||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:YGL102C(YGL102C_d)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:YGR115C(YGR115C_d)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YKL153W(YKL153W_d)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YRB1(YDR002W)|FD-Score:4.41|P-value:5.20E-6|Clearance:0.05||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:ZIM17(YNL310C)|FD-Score:4.35|P-value:6.67E-6|Clearance:0.03||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17590849
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:AFG3(YER017C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM11(YER093C-A)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ALG3(YBL082C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins Gene:ARD1(YHR013C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARG5,6(YER069W)|FD-Score:-6.46|P-value:5.11E-11||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ASI2(YNL159C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATG14(YBR128C)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:ATP25(YMR098C)|FD-Score:4.81|P-value:7.40E-7||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BDF2(YDL070W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BNI1(YNL271C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTS1(YPL069C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD30(YDL151C_d)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CLB2(YPR119W)|FD-Score:4.93|P-value:4.10E-7||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CNM67(YNL225C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COT1(YOR316C)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:COX19(YLL018C-A)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DAL7(YIR031C)|FD-Score:5.37|P-value:3.96E-8||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAP1(YPL170W)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DHH1(YDL160C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DJP1(YIR004W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DOG2(YHR043C)|FD-Score:-5.09|P-value:1.83E-7||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DOM34(YNL001W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DPH2(YKL191W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p Gene:ERI1(YPL096C-A)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:ERV46(YAL042W)|FD-Score:6.21|P-value:2.67E-10||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FMC1(YIL098C)|FD-Score:-5.17|P-value:1.20E-7||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:FMS1(YMR020W)|FD-Score:4.41|P-value:5.26E-6||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:FZF1(YGL254W)|FD-Score:-7.5|P-value:3.11E-14||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GCS1(YDL226C)|FD-Score:-7.67|P-value:8.94E-15||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDT1(YBR187W_p)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GEA1(YJR031C)|FD-Score:-4.3|P-value:8.48E-6||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GPT2(YKR067W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GSH1(YJL101C)|FD-Score:7|P-value:1.30E-12||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GTT3(YEL017W)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HIT1(YJR055W)|FD-Score:6.03|P-value:8.27E-10||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOM3(YER052C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC4(YDR540C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IXR1(YKL032C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:JEN1(YKL217W)|FD-Score:-6.66|P-value:1.37E-11||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:JNM1(YMR294W)|FD-Score:-4.34|P-value:7.24E-6||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KTI11(YBL071W-A)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LDB16(YCL005W)|FD-Score:-4.09|P-value:2.19E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LDB19(YOR322C)|FD-Score:5.4|P-value:3.37E-8||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:MDH3(YDL078C)|FD-Score:-4.28|P-value:9.41E-6||SGD DESC:Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle Gene:MDJ1(YFL016C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDL2(YPL270W)|FD-Score:4.57|P-value:2.50E-6||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MDM38(YOL027C)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MET3(YJR010W)|FD-Score:7.95|P-value:9.22E-16||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MIG3(YER028C)|FD-Score:4.51|P-value:3.20E-6||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MMT2(YPL224C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MPH1(YIR002C)|FD-Score:4.3|P-value:8.72E-6||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRK1(YDL079C)|FD-Score:11.2|P-value:1.62E-29||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL40(YPL173W)|FD-Score:3.82|P-value:6.54E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NBP2(YDR162C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NCL1(YBL024W)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NNT1(YLR285W)|FD-Score:4.77|P-value:9.21E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NSR1(YGR159C)|FD-Score:-4.8|P-value:7.87E-7||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:NUP53(YMR153W)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OCA2(YNL056W)|FD-Score:-4.03|P-value:2.84E-5||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OPT2(YPR194C)|FD-Score:-9.95|P-value:1.32E-23||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PDH1(YPR002W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDR16(YNL231C)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PDR17(YNL264C)|FD-Score:-3.84|P-value:6.24E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Gene:PET10(YKR046C)|FD-Score:6.01|P-value:9.31E-10||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PET130(YJL023C)|FD-Score:-3.97|P-value:3.52E-5||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PLB1(YMR008C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PLB3(YOL011W)|FD-Score:5.89|P-value:1.92E-9||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMT1(YDL095W)|FD-Score:-4.46|P-value:4.10E-6||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:POL4(YCR014C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:PPM1(YDR435C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PPZ1(YML016C)|FD-Score:-3.87|P-value:5.38E-5||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRM4(YPL156C)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PRY3(YJL078C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:PSY3(YLR376C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:QCR9(YGR183C)|FD-Score:5.87|P-value:2.13E-9||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RBG1(YAL036C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RCK1(YGL158W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RCN2(YOR220W_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RFS1(YBR052C)|FD-Score:4.54|P-value:2.88E-6||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RHO5(YNL180C)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RIB4(YOL143C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RIM20(YOR275C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RMD6(YEL072W)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Protein required for sporulation Gene:ROG3(YFR022W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:ROY1(YMR258C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL20A(YMR242C)|FD-Score:4.97|P-value:3.34E-7||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPS17B(YDR447C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRT16(YNL105W_d)|FD-Score:8.31|P-value:4.59E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Gene:RTG2(YGL252C)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RXT2(YBR095C)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC7(YDR389W)|FD-Score:-5.32|P-value:5.30E-8||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SDP1(YIL113W)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SET5(YHR207C)|FD-Score:4.77|P-value:9.01E-7||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SFC1(YJR095W)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SHE4(YOR035C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SPT23(YKL020C)|FD-Score:5.49|P-value:2.01E-8||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SRO77(YBL106C)|FD-Score:-4.53|P-value:2.97E-6||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SRO9(YCL037C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSE1(YPL106C)|FD-Score:-3.81|P-value:7.01E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSH1(YBR283C)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SSO2(YMR183C)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:TAT2(YOL020W)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TED1(YIL039W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TIF4631(YGR162W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TIM21(YGR033C)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TMA108(YIL137C)|FD-Score:6.22|P-value:2.45E-10||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TMA23(YMR269W)|FD-Score:5.36|P-value:4.08E-8||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TMN2(YDR107C)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments Gene:TMS1(YDR105C)|FD-Score:6.52|P-value:3.49E-11||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TOM5(YPR133W-A)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TPN1(YGL186C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSC3(YBR058C-A)|FD-Score:9.6|P-value:4.02E-22||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA4(YHR111W)|FD-Score:-3.81|P-value:6.84E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UTH1(YKR042W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VID30(YGL227W)|FD-Score:-5.38|P-value:3.82E-8||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VMA3(YEL027W)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS3(YDR495C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS69(YPR087W_d)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:VPS71(YML041C)|FD-Score:8.53|P-value:7.24E-18||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS9(YML097C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBR012C(YBR012C_d)|FD-Score:3.84|P-value:6.03E-5||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR225W(YBR225W_p)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YCF1(YDR135C)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YCL042W(YCL042W_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR043C(YCR043C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.91|P-value:4.52E-5||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL032W(YDL032W_d)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YER097W(YER097W_d)|FD-Score:-4.02|P-value:2.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL040W(YFL040W_p)|FD-Score:-6.13|P-value:4.53E-10||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGR035W-A(YGR035W-A_p)|FD-Score:9.03|P-value:8.75E-20||SGD DESC:Putative protein of unknown function Gene:YGR130C(YGR130C)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YHR035W(YHR035W_p)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YHR140W(YHR140W_p)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YIL024C(YIL024C_p)|FD-Score:4|P-value:3.15E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YIL067C(YIL067C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL077W-B(YJL077W-B_p)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL120W(YJL120W_d)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL127C-B(YJL127C-B_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i> Gene:YJL163C(YJL163C_p)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Putative protein of unknown function Gene:YLL006W-A(YLL006W-A_p)|FD-Score:4.34|P-value:7.12E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLL017W(YLL017W)|FD-Score:7.95|P-value:9.52E-16||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:YLR184W(YLR184W_d)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR194C(YLR194C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR211C(YLR211C_p)|FD-Score:-5.07|P-value:1.98E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR225C(YLR225C_p)|FD-Score:6.8|P-value:5.06E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR346C(YLR346C_p)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Putative protein of unknown function Gene:YNL115C(YNL115C_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YOR114W(YOR114W_p)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR131C(YOR131C_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOS9(YDR057W)|FD-Score:4.81|P-value:7.65E-7||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPR123C(YPR123C_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:AAC3(YBR085W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:AFG3(YER017C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM11(YER093C-A)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ALG3(YBL082C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins Gene:ARD1(YHR013C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARG5,6(YER069W)|FD-Score:-6.46|P-value:5.11E-11||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ASI2(YNL159C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATG14(YBR128C)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:ATP25(YMR098C)|FD-Score:4.81|P-value:7.40E-7||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BDF2(YDL070W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:BNI1(YNL271C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BTS1(YPL069C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD30(YDL151C_d)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CLB2(YPR119W)|FD-Score:4.93|P-value:4.10E-7||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CNM67(YNL225C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COT1(YOR316C)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:COX19(YLL018C-A)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DAL7(YIR031C)|FD-Score:5.37|P-value:3.96E-8||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAP1(YPL170W)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DHH1(YDL160C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DJP1(YIR004W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DOG2(YHR043C)|FD-Score:-5.09|P-value:1.83E-7||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DOM34(YNL001W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DPH2(YKL191W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p Gene:ERI1(YPL096C-A)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:ERV46(YAL042W)|FD-Score:6.21|P-value:2.67E-10||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FMC1(YIL098C)|FD-Score:-5.17|P-value:1.20E-7||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:FMS1(YMR020W)|FD-Score:4.41|P-value:5.26E-6||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:FZF1(YGL254W)|FD-Score:-7.5|P-value:3.11E-14||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GCS1(YDL226C)|FD-Score:-7.67|P-value:8.94E-15||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDT1(YBR187W_p)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GEA1(YJR031C)|FD-Score:-4.3|P-value:8.48E-6||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GPT2(YKR067W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GSH1(YJL101C)|FD-Score:7|P-value:1.30E-12||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GTT3(YEL017W)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HIT1(YJR055W)|FD-Score:6.03|P-value:8.27E-10||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOM3(YER052C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC4(YDR540C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IXR1(YKL032C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:JEN1(YKL217W)|FD-Score:-6.66|P-value:1.37E-11||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:JNM1(YMR294W)|FD-Score:-4.34|P-value:7.24E-6||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KTI11(YBL071W-A)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LDB16(YCL005W)|FD-Score:-4.09|P-value:2.19E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LDB19(YOR322C)|FD-Score:5.4|P-value:3.37E-8||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:MDH3(YDL078C)|FD-Score:-4.28|P-value:9.41E-6||SGD DESC:Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle Gene:MDJ1(YFL016C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDL2(YPL270W)|FD-Score:4.57|P-value:2.50E-6||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MDM38(YOL027C)|FD-Score:-3.1|P-value:9.76E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MET3(YJR010W)|FD-Score:7.95|P-value:9.22E-16||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MIG3(YER028C)|FD-Score:4.51|P-value:3.20E-6||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MMT2(YPL224C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MPH1(YIR002C)|FD-Score:4.3|P-value:8.72E-6||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRK1(YDL079C)|FD-Score:11.2|P-value:1.62E-29||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL40(YPL173W)|FD-Score:3.82|P-value:6.54E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NBP2(YDR162C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NCL1(YBL024W)|FD-Score:4.32|P-value:7.84E-6||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NNT1(YLR285W)|FD-Score:4.77|P-value:9.21E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NSR1(YGR159C)|FD-Score:-4.8|P-value:7.87E-7||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:NUP53(YMR153W)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OCA2(YNL056W)|FD-Score:-4.03|P-value:2.84E-5||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OPT2(YPR194C)|FD-Score:-9.95|P-value:1.32E-23||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PDH1(YPR002W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDR16(YNL231C)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PDR17(YNL264C)|FD-Score:-3.84|P-value:6.24E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Gene:PET10(YKR046C)|FD-Score:6.01|P-value:9.31E-10||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PET130(YJL023C)|FD-Score:-3.97|P-value:3.52E-5||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PLB1(YMR008C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PLB3(YOL011W)|FD-Score:5.89|P-value:1.92E-9||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMT1(YDL095W)|FD-Score:-4.46|P-value:4.10E-6||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:POL4(YCR014C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:PPM1(YDR435C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PPZ1(YML016C)|FD-Score:-3.87|P-value:5.38E-5||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRM4(YPL156C)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PRY3(YJL078C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:PSY3(YLR376C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:QCR9(YGR183C)|FD-Score:5.87|P-value:2.13E-9||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RBG1(YAL036C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RCK1(YGL158W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RCN2(YOR220W_p)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RFS1(YBR052C)|FD-Score:4.54|P-value:2.88E-6||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RHO5(YNL180C)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RIB4(YOL143C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RIM20(YOR275C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RMD6(YEL072W)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Protein required for sporulation Gene:ROG3(YFR022W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:ROY1(YMR258C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL20A(YMR242C)|FD-Score:4.97|P-value:3.34E-7||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPS17B(YDR447C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRT16(YNL105W_d)|FD-Score:8.31|P-value:4.59E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Gene:RTG2(YGL252C)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RXT2(YBR095C)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC7(YDR389W)|FD-Score:-5.32|P-value:5.30E-8||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SDP1(YIL113W)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SET5(YHR207C)|FD-Score:4.77|P-value:9.01E-7||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SFC1(YJR095W)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SHE4(YOR035C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SPT23(YKL020C)|FD-Score:5.49|P-value:2.01E-8||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SRO77(YBL106C)|FD-Score:-4.53|P-value:2.97E-6||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SRO9(YCL037C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSE1(YPL106C)|FD-Score:-3.81|P-value:7.01E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSH1(YBR283C)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SSO2(YMR183C)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:TAT2(YOL020W)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TED1(YIL039W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TIF4631(YGR162W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TIM21(YGR033C)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TMA108(YIL137C)|FD-Score:6.22|P-value:2.45E-10||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TMA23(YMR269W)|FD-Score:5.36|P-value:4.08E-8||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TMN2(YDR107C)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments Gene:TMS1(YDR105C)|FD-Score:6.52|P-value:3.49E-11||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TOM5(YPR133W-A)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TPN1(YGL186C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSC3(YBR058C-A)|FD-Score:9.6|P-value:4.02E-22||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA4(YHR111W)|FD-Score:-3.81|P-value:6.84E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UTH1(YKR042W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VID30(YGL227W)|FD-Score:-5.38|P-value:3.82E-8||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VMA3(YEL027W)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS3(YDR495C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS69(YPR087W_d)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:VPS71(YML041C)|FD-Score:8.53|P-value:7.24E-18||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS9(YML097C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBR012C(YBR012C_d)|FD-Score:3.84|P-value:6.03E-5||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR225W(YBR225W_p)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YCF1(YDR135C)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YCL042W(YCL042W_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR043C(YCR043C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.91|P-value:4.52E-5||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL032W(YDL032W_d)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YER097W(YER097W_d)|FD-Score:-4.02|P-value:2.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL040W(YFL040W_p)|FD-Score:-6.13|P-value:4.53E-10||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGR035W-A(YGR035W-A_p)|FD-Score:9.03|P-value:8.75E-20||SGD DESC:Putative protein of unknown function Gene:YGR130C(YGR130C)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YHR035W(YHR035W_p)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YHR140W(YHR140W_p)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YIL024C(YIL024C_p)|FD-Score:4|P-value:3.15E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YIL067C(YIL067C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL077W-B(YJL077W-B_p)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL120W(YJL120W_d)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL127C-B(YJL127C-B_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i> Gene:YJL163C(YJL163C_p)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Putative protein of unknown function Gene:YLL006W-A(YLL006W-A_p)|FD-Score:4.34|P-value:7.12E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLL017W(YLL017W)|FD-Score:7.95|P-value:9.52E-16||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:YLR184W(YLR184W_d)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR194C(YLR194C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR211C(YLR211C_p)|FD-Score:-5.07|P-value:1.98E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR225C(YLR225C_p)|FD-Score:6.8|P-value:5.06E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YLR346C(YLR346C_p)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Putative protein of unknown function Gene:YNL115C(YNL115C_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YOR114W(YOR114W_p)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YOR131C(YOR131C_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOS9(YDR057W)|FD-Score:4.81|P-value:7.65E-7||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPR123C(YPR123C_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL151C5.619.91E-90.50RPC31RNA polymerase III subunit C31
YGL122C5.611.03E-80.50NAB2Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress
YBR254C5.101.67E-70.27TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
YDL132W4.836.66E-70.07CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YML092C4.779.38E-70.11PRE8Alpha 2 subunit of the 20S proteasome
YLR166C4.651.62E-60.12SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YLR066W4.542.83E-60.13SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YDR002W4.415.20E-60.05YRB1Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
YNL310C4.356.67E-60.03ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YDR228C4.337.59E-60.01PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YPR103W4.327.90E-60.18PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YAL003W4.131.78E-50.15EFB1Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
YDR510W3.983.38E-50.09SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
YPL153C3.894.98E-50.07RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YDL102W3.826.67E-50.22POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL079C11.201.62E-29MRK1Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation
YBR058C-A9.604.02E-22TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGR035W-A_p9.038.75E-20YGR035W-A_pPutative protein of unknown function
YML041C8.537.24E-18VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YNL105W_d8.314.59E-17RRT16_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
YJR010W7.959.22E-16MET3ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism
YLL017W7.959.52E-16YLL017WNon-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
YMR183C7.331.16E-13SSO2Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p
YJL101C7.001.30E-12GSH1Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
YLR225C_p6.805.06E-12YLR225C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene
YDR105C6.523.49E-11TMS1Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
YIL137C6.222.45E-10TMA108Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease
YAL042W6.212.67E-10ERV46Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport
YJR055W6.038.27E-10HIT1Protein of unknown function, required for growth at high temperature
YKR046C6.019.31E-10PET10Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange

GO enrichment analysis for SGTC_3210
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1291.85E-23SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.060241TSC3-RPN4
0.1219.36E-21SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0289855TSC3-RPN4
0.1202.60E-20SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0581395TSC3-RPN4
0.1172.43E-19SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0657895TSC3-RPN4
0.1141.37E-18SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0675676TSC3-RPN4
0.1119.17E-18SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0952381TSC3-RPN4
0.1103.17E-17SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0588235TSC3-RPN4
0.1095.18E-17SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0735294TSC3-RPN4
0.1072.19E-16SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0757576
0.1041.13E-15SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0434783TSC3-RPN4
0.1031.93E-15SGTC_13321480-0653 17.5 μMChemDiv (Drug-like library)57494380.0895522TSC3-RPN4
0.1032.74E-15SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0555556TSC3-RPN4
0.1023.18E-15SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0757576TSC3-RPN4
0.1001.14E-14SGTC_1891st059073 26.2 μMTimTec (Natural product derivative library)6887540.0625TSC3-RPN4
0.0984.42E-14SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0428571TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2891906387619.48 μM0.6037746472478Chembridge (Drug-like library)312.346382.79206
SGTC_3117912494549.47 μM0.58490617592065Chembridge (Drug-like library)311.361622.89706
SGTC_3068911676849.47 μM0.541904888Chembridge (Drug-like library)286.395223.78404
SGTC_3307912688569.28 μM0.43636424824909Chembridge (Drug-like library)272.368643.77904
SGTC_2996906619271.43 μM0.3965526472854Chembridge (Drug-like library)326.3481433.41906
SGTC_3102912166949.47 μM0.39285741905072Chembridge (Drug-like library)272.368643.57504
SGTC_3292912082559.78 μM0.38596524824900Chembridge (Drug-like library)276.3325233.29405
SGTC_3069911700949.47 μM0.3793141904981Chembridge (Drug-like library)284.379343.7610460S ribosome export
SGTC_3101912053149.47 μM0.37704924824904Chembridge (Drug-like library)288.368043.27705
SGTC_3306912767659.79 μM0.36842141905287Chembridge (Drug-like library)264.369783.04205