9128307

5-amino-1-(3-bromophenyl)-N-cyclopentyltriazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3212
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 27243542
SMILES C1CCC(C1)NC(=O)C2=C(N(N=N2)C3=CC(=CC=C3)Br)N
Standardized SMILES Nc1c(nnn1c2cccc(Br)c2)C(=O)NC3CCCC3
Molecular weight 350.2137
ALogP 2.75
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.42
% growth inhibition (Hom. pool) 9.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 27243542
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:-5.31|P-value:5.46E-8|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ACT1(YFL039C)|FD-Score:4.86|P-value:5.98E-7|Clearance:0.46||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ATP16(YDL004W)|FD-Score:-7.93|P-value:1.07E-15|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CCT4(YDL143W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC1(YDR182W)|FD-Score:4.17|P-value:1.50E-5|Clearance:0.08||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:CDC25(YLR310C)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.07||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC8(YJR057W)|FD-Score:-3.15|P-value:8.06E-4|Clearance:0||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CKS1(YBR135W)|FD-Score:3.24|P-value:6.00E-4|Clearance:0||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:COG3(YER157W)|FD-Score:-7.46|P-value:4.35E-14|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWC25(YNL245C)|FD-Score:-5.79|P-value:3.59E-9|Clearance:0||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DSL1(YNL258C)|FD-Score:-3.56|P-value:1.88E-4|Clearance:0||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:GCD10(YNL062C)|FD-Score:4.3|P-value:8.46E-6|Clearance:0.12||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GPN3(YLR243W_p)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.12||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:ILV3(YJR016C)|FD-Score:-6.31|P-value:1.37E-10|Clearance:0||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:LAS1(YKR063C)|FD-Score:-3.16|P-value:7.94E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MAS2(YHR024C)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.17||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MSL5(YLR116W)|FD-Score:4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NOP1(YDL014W)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.11||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NSE5(YML023C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PDC2(YDR081C)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.05||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PKC1(YBL105C)|FD-Score:3.74|P-value:9.11E-5|Clearance:0.11||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POB3(YML069W)|FD-Score:6.09|P-value:5.68E-10|Clearance:0.03||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:PRE1(YER012W)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.07||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RET1(YOR207C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.01||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RHO3(YIL118W)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.03||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RIO2(YNL207W)|FD-Score:-3.17|P-value:7.66E-4|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPA43(YOR340C)|FD-Score:11.3|P-value:4.69E-30|Clearance:5.24||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:4.03|P-value:2.84E-5|Clearance:0.28||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPP1(YHR062C)|FD-Score:4.39|P-value:5.62E-6|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC23(YPR181C)|FD-Score:6.06|P-value:6.80E-10|Clearance:0.52||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TFC4(YGR047C)|FD-Score:-3.36|P-value:3.91E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:TOA1(YOR194C)|FD-Score:5.54|P-value:1.53E-8|Clearance:0.68||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:YDR413C(YDR413C_d)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YHC1(YLR298C)|FD-Score:-3.51|P-value:2.28E-4|Clearance:0||SGD DESC:Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site Gene:ACP1(YKL192C)|FD-Score:-5.31|P-value:5.46E-8|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ACT1(YFL039C)|FD-Score:4.86|P-value:5.98E-7|Clearance:0.46||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ATP16(YDL004W)|FD-Score:-7.93|P-value:1.07E-15|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CCT4(YDL143W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC1(YDR182W)|FD-Score:4.17|P-value:1.50E-5|Clearance:0.08||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:CDC25(YLR310C)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.07||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC8(YJR057W)|FD-Score:-3.15|P-value:8.06E-4|Clearance:0||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CKS1(YBR135W)|FD-Score:3.24|P-value:6.00E-4|Clearance:0||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:COG3(YER157W)|FD-Score:-7.46|P-value:4.35E-14|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWC25(YNL245C)|FD-Score:-5.79|P-value:3.59E-9|Clearance:0||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DSL1(YNL258C)|FD-Score:-3.56|P-value:1.88E-4|Clearance:0||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:GCD10(YNL062C)|FD-Score:4.3|P-value:8.46E-6|Clearance:0.12||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GPN3(YLR243W_p)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.12||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:ILV3(YJR016C)|FD-Score:-6.31|P-value:1.37E-10|Clearance:0||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:LAS1(YKR063C)|FD-Score:-3.16|P-value:7.94E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MAS2(YHR024C)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.17||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MSL5(YLR116W)|FD-Score:4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NOP1(YDL014W)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.11||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NSE5(YML023C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PDC2(YDR081C)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.05||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PKC1(YBL105C)|FD-Score:3.74|P-value:9.11E-5|Clearance:0.11||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POB3(YML069W)|FD-Score:6.09|P-value:5.68E-10|Clearance:0.03||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:PRE1(YER012W)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.07||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RET1(YOR207C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.01||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RHO3(YIL118W)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.03||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RIO2(YNL207W)|FD-Score:-3.17|P-value:7.66E-4|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPA43(YOR340C)|FD-Score:11.3|P-value:4.69E-30|Clearance:5.24||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:4.03|P-value:2.84E-5|Clearance:0.28||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPP1(YHR062C)|FD-Score:4.39|P-value:5.62E-6|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC23(YPR181C)|FD-Score:6.06|P-value:6.80E-10|Clearance:0.52||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:TFC4(YGR047C)|FD-Score:-3.36|P-value:3.91E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:TOA1(YOR194C)|FD-Score:5.54|P-value:1.53E-8|Clearance:0.68||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:YDR413C(YDR413C_d)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YHC1(YLR298C)|FD-Score:-3.51|P-value:2.28E-4|Clearance:0||SGD DESC:Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 27243542
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AFT1(YGL071W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIF1(YNR074C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:AIM45(YPR004C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:AKR2(YOR034C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:APP1(YNL094W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARO8(YGL202W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis Gene:AST2(YER101C)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:ATG26(YLR189C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:ATG32(YIL146C)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATP3(YBR039W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT2(YEL064C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AYT1(YLL063C)|FD-Score:-4.56|P-value:2.58E-6||SGD DESC:Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis Gene:BER1(YLR412W)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BOI1(YBL085W)|FD-Score:4.29|P-value:8.95E-6||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BOP3(YNL042W)|FD-Score:-3.74|P-value:9.18E-5||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:BUD26(YDR241W_d)|FD-Score:5.58|P-value:1.18E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAN1(YEL063C)|FD-Score:8.79|P-value:7.44E-19||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CBF1(YJR060W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CKA1(YIL035C)|FD-Score:-5.04|P-value:2.30E-7||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CPD1(YGR247W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CRZ1(YNL027W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress Gene:CUL3(YGR003W)|FD-Score:-5.15|P-value:1.27E-7||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:CWH41(YGL027C)|FD-Score:4.46|P-value:4.15E-6||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAL4(YIR028W)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DEF1(YKL054C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DFG16(YOR030W)|FD-Score:-5.65|P-value:8.05E-9||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DGK1(YOR311C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DSS4(YPR017C)|FD-Score:4.41|P-value:5.12E-6||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EEB1(YPL095C)|FD-Score:-5.33|P-value:4.92E-8||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EMI5(YOL071W)|FD-Score:-3.96|P-value:3.76E-5||SGD DESC:Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) Gene:ESBP6(YNL125C)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:EST2(YLR318W)|FD-Score:4.94|P-value:3.90E-7||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FAU1(YER183C)|FD-Score:-4.85|P-value:6.14E-7||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FUI1(YBL042C)|FD-Score:7.39|P-value:7.35E-14||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAT1(YFL021W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p Gene:GDH3(YAL062W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HES1(YOR237W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:HRQ1(YDR291W_p)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IMP1(YMR150C)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IMP2'(YIL154C)|FD-Score:5.71|P-value:5.77E-9||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INO1(YJL153C)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:INO4(YOL108C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:KIN3(YAR018C)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:KSP1(YHR082C)|FD-Score:-3.99|P-value:3.32E-5||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:KTR6(YPL053C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LAG1(YHL003C)|FD-Score:14.7|P-value:3.13E-49||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LCL1(YPL056C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LDB17(YDL146W)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LPD1(YFL018C)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MAK10(YEL053C)|FD-Score:-4.07|P-value:2.33E-5||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MBF1(YOR298C-A)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MCR1(YKL150W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MMS22(YLR320W)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNL1(YHR204W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MNN4(YKL201C)|FD-Score:4.32|P-value:7.69E-6||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MRP10(YDL045W-A)|FD-Score:5.46|P-value:2.43E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPL15(YLR312W-A)|FD-Score:6.51|P-value:3.75E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL35(YDR322W)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSS1(YMR023C)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:MST1(YKL194C)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC5(YDR128W)|FD-Score:-4.98|P-value:3.18E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:OCA2(YNL056W)|FD-Score:6.64|P-value:1.53E-11||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OPI3(YJR073C)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAR32(YDL173W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEX18(YHR160C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PHO4(YFR034C)|FD-Score:7.06|P-value:8.14E-13||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PIN2(YOR104W)|FD-Score:6.38|P-value:9.10E-11||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PIN4(YBL051C)|FD-Score:-3.22|P-value:6.52E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PRC1(YMR297W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRR1(YKL116C)|FD-Score:5.31|P-value:5.38E-8||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PRS4(YBL068W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:QDR3(YBR043C)|FD-Score:-7.65|P-value:1.01E-14||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RCO1(YMR075W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:REG1(YDR028C)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REV3(YPL167C)|FD-Score:14.9|P-value:2.70E-50||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIM8(YGL045W)|FD-Score:7.91|P-value:1.27E-15||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPA49(YNL248C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPH1(YER169W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL4A(YBR031W)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS23B(YPR132W)|FD-Score:-7.09|P-value:6.61E-13||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS9B(YBR189W)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:6.11|P-value:4.84E-10||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RSM7(YJR113C)|FD-Score:4.37|P-value:6.32E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTT107(YHR154W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:9.13|P-value:3.33E-20||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEE1(YIL064W)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SIC1(YLR079W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKI3(YPR189W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLM2(YNL047C)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication Gene:SNF2(YOR290C)|FD-Score:4.31|P-value:8.20E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOM1(YEL059C-A)|FD-Score:-4.71|P-value:1.23E-6||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SPE4(YLR146C)|FD-Score:-3.92|P-value:4.45E-5||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SPR1(YOR190W)|FD-Score:4.3|P-value:8.65E-6||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SPT23(YKL020C)|FD-Score:-4.36|P-value:6.36E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SRY1(YKL218C)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SUB1(YMR039C)|FD-Score:-4.31|P-value:8.22E-6||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SUR4(YLR372W)|FD-Score:5.63|P-value:9.17E-9||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SVP26(YHR181W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:THI73(YLR004C)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs Gene:TIM8(YJR135W-A)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TRP4(YDR354W)|FD-Score:-5.14|P-value:1.39E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRX1(YLR043C)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBS1(YBR165W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:URA8(YJR103W)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VFA1(YER128W)|FD-Score:-3.19|P-value:6.99E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMA11(YPL234C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS33(YLR396C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:YBR062C(YBR062C_p)|FD-Score:-4.99|P-value:3.06E-7||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR144C(YBR144C_d)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Gene:YCL046W(YCL046W_d)|FD-Score:-4.87|P-value:5.50E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR061W(YCR061W_p)|FD-Score:7.24|P-value:2.32E-13||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDC1(YPL087W)|FD-Score:5.4|P-value:3.26E-8||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL027C(YDL027C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDL162C(YDL162C_d)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDL218W(YDL218W_p)|FD-Score:-5.3|P-value:5.90E-8||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR220C(YDR220C_d)|FD-Score:-4.5|P-value:3.44E-6||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YEL057C(YEL057C_p)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER077C(YER077C_p)|FD-Score:5.27|P-value:6.74E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YFL012W(YFL012W_p)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-4.98|P-value:3.18E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGK3(YOL128C)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL082W(YGL082W_p)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGL218W(YGL218W_d)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR111W(YGR111W_p)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR130C(YHR130C_d)|FD-Score:7.14|P-value:4.60E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:-5.1|P-value:1.66E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL096C(YIL096C_p)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YIP4(YGL198W)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR079W(YJR079W_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YJR107W(YJR107W_p)|FD-Score:5.91|P-value:1.68E-9||SGD DESC:Putative lipase Gene:YKR045C(YKR045C_p)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR012C(YLR012C_p)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR118C(YLR118C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR194C(YLR194C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR294C(YLR294C_d)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YLR365W(YLR365W_d)|FD-Score:-4.36|P-value:6.49E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:-6.26|P-value:1.92E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YLR444C(YLR444C_d)|FD-Score:-7.28|P-value:1.63E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR084W(YMR084W_p)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR122C(YMR122C_d)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR147W(YMR147W_p)|FD-Score:-5.16|P-value:1.21E-7||SGD DESC:Putative protein of unknown function Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR221C(YMR221C_p)|FD-Score:-3.13|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YNL200C(YNL200C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR055W(YOR055W_d)|FD-Score:6.66|P-value:1.36E-11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR062C(YOR062C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YOR111W(YOR111W_p)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Putative protein of unknown function Gene:YPT11(YNL304W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:YSC83(YHR017W)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:ADO1(YJR105W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AFT1(YGL071W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIF1(YNR074C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase Gene:AIM45(YPR004C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:AKR2(YOR034C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:APP1(YNL094W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARO8(YGL202W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis Gene:AST2(YER101C)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:ATG26(YLR189C)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:ATG32(YIL146C)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATP3(YBR039W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT2(YEL064C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AYT1(YLL063C)|FD-Score:-4.56|P-value:2.58E-6||SGD DESC:Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis Gene:BER1(YLR412W)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BOI1(YBL085W)|FD-Score:4.29|P-value:8.95E-6||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BOP3(YNL042W)|FD-Score:-3.74|P-value:9.18E-5||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:BUD26(YDR241W_d)|FD-Score:5.58|P-value:1.18E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAN1(YEL063C)|FD-Score:8.79|P-value:7.44E-19||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CBF1(YJR060W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CKA1(YIL035C)|FD-Score:-5.04|P-value:2.30E-7||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CPD1(YGR247W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CRZ1(YNL027W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress Gene:CUL3(YGR003W)|FD-Score:-5.15|P-value:1.27E-7||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:CWH41(YGL027C)|FD-Score:4.46|P-value:4.15E-6||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAL4(YIR028W)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DEF1(YKL054C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DFG16(YOR030W)|FD-Score:-5.65|P-value:8.05E-9||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DGK1(YOR311C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DSS4(YPR017C)|FD-Score:4.41|P-value:5.12E-6||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:EEB1(YPL095C)|FD-Score:-5.33|P-value:4.92E-8||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EMI5(YOL071W)|FD-Score:-3.96|P-value:3.76E-5||SGD DESC:Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) Gene:ESBP6(YNL125C)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:EST2(YLR318W)|FD-Score:4.94|P-value:3.90E-7||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FAU1(YER183C)|FD-Score:-4.85|P-value:6.14E-7||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FUI1(YBL042C)|FD-Score:7.39|P-value:7.35E-14||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAT1(YFL021W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p Gene:GDH3(YAL062W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HES1(YOR237W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:HRQ1(YDR291W_p)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IMP1(YMR150C)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IMP2'(YIL154C)|FD-Score:5.71|P-value:5.77E-9||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INO1(YJL153C)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:INO4(YOL108C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:KIN3(YAR018C)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:KSP1(YHR082C)|FD-Score:-3.99|P-value:3.32E-5||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:KTR6(YPL053C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LAG1(YHL003C)|FD-Score:14.7|P-value:3.13E-49||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LCL1(YPL056C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LDB17(YDL146W)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LPD1(YFL018C)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MAK10(YEL053C)|FD-Score:-4.07|P-value:2.33E-5||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MBF1(YOR298C-A)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MCR1(YKL150W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MMS22(YLR320W)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNL1(YHR204W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MNN4(YKL201C)|FD-Score:4.32|P-value:7.69E-6||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MRP10(YDL045W-A)|FD-Score:5.46|P-value:2.43E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPL15(YLR312W-A)|FD-Score:6.51|P-value:3.75E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL35(YDR322W)|FD-Score:5.14|P-value:1.40E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSS1(YMR023C)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:MST1(YKL194C)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC5(YDR128W)|FD-Score:-4.98|P-value:3.18E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:OCA2(YNL056W)|FD-Score:6.64|P-value:1.53E-11||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OPI3(YJR073C)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAR32(YDL173W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEX18(YHR160C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PHO4(YFR034C)|FD-Score:7.06|P-value:8.14E-13||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PIN2(YOR104W)|FD-Score:6.38|P-value:9.10E-11||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PIN4(YBL051C)|FD-Score:-3.22|P-value:6.52E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PRC1(YMR297W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRR1(YKL116C)|FD-Score:5.31|P-value:5.38E-8||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PRS4(YBL068W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:QDR3(YBR043C)|FD-Score:-7.65|P-value:1.01E-14||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RCO1(YMR075W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:REG1(YDR028C)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REV3(YPL167C)|FD-Score:14.9|P-value:2.70E-50||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIM8(YGL045W)|FD-Score:7.91|P-value:1.27E-15||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPA49(YNL248C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPH1(YER169W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL4A(YBR031W)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS23B(YPR132W)|FD-Score:-7.09|P-value:6.61E-13||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS9B(YBR189W)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:6.11|P-value:4.84E-10||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RSM7(YJR113C)|FD-Score:4.37|P-value:6.32E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTT107(YHR154W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:9.13|P-value:3.33E-20||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEE1(YIL064W)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SIC1(YLR079W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKI3(YPR189W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SLM2(YNL047C)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication Gene:SNF2(YOR290C)|FD-Score:4.31|P-value:8.20E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOM1(YEL059C-A)|FD-Score:-4.71|P-value:1.23E-6||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SPE4(YLR146C)|FD-Score:-3.92|P-value:4.45E-5||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SPR1(YOR190W)|FD-Score:4.3|P-value:8.65E-6||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SPT23(YKL020C)|FD-Score:-4.36|P-value:6.36E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SRY1(YKL218C)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SUB1(YMR039C)|FD-Score:-4.31|P-value:8.22E-6||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SUR4(YLR372W)|FD-Score:5.63|P-value:9.17E-9||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SVP26(YHR181W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:THI73(YLR004C)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs Gene:TIM8(YJR135W-A)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TRP4(YDR354W)|FD-Score:-5.14|P-value:1.39E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRX1(YLR043C)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBS1(YBR165W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:URA8(YJR103W)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VFA1(YER128W)|FD-Score:-3.19|P-value:6.99E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMA11(YPL234C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS33(YLR396C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:YBR062C(YBR062C_p)|FD-Score:-4.99|P-value:3.06E-7||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR144C(YBR144C_d)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Gene:YCL046W(YCL046W_d)|FD-Score:-4.87|P-value:5.50E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR061W(YCR061W_p)|FD-Score:7.24|P-value:2.32E-13||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDC1(YPL087W)|FD-Score:5.4|P-value:3.26E-8||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL027C(YDL027C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDL162C(YDL162C_d)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDL218W(YDL218W_p)|FD-Score:-5.3|P-value:5.90E-8||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR220C(YDR220C_d)|FD-Score:-4.5|P-value:3.44E-6||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YEL057C(YEL057C_p)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER077C(YER077C_p)|FD-Score:5.27|P-value:6.74E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YFL012W(YFL012W_p)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-4.98|P-value:3.18E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGK3(YOL128C)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL082W(YGL082W_p)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGL218W(YGL218W_d)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR111W(YGR111W_p)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR130C(YHR130C_d)|FD-Score:7.14|P-value:4.60E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:-5.1|P-value:1.66E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL096C(YIL096C_p)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YIP4(YGL198W)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR079W(YJR079W_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YJR107W(YJR107W_p)|FD-Score:5.91|P-value:1.68E-9||SGD DESC:Putative lipase Gene:YKR045C(YKR045C_p)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR012C(YLR012C_p)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR118C(YLR118C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR194C(YLR194C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR294C(YLR294C_d)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YLR365W(YLR365W_d)|FD-Score:-4.36|P-value:6.49E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:-6.26|P-value:1.92E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YLR444C(YLR444C_d)|FD-Score:-7.28|P-value:1.63E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR084W(YMR084W_p)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR122C(YMR122C_d)|FD-Score:3.73|P-value:9.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR147W(YMR147W_p)|FD-Score:-5.16|P-value:1.21E-7||SGD DESC:Putative protein of unknown function Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR221C(YMR221C_p)|FD-Score:-3.13|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YNL200C(YNL200C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR055W(YOR055W_d)|FD-Score:6.66|P-value:1.36E-11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR062C(YOR062C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YOR111W(YOR111W_p)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Putative protein of unknown function Gene:YPT11(YNL304W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:YSC83(YHR017W)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR340C11.304.69E-305.24RPA43RNA polymerase I subunit A43
YML069W6.095.68E-100.03POB3Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
YPR181C6.066.80E-100.52SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YOR194C5.541.53E-80.68TOA1TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
YFL039C4.865.98E-70.47ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YHR062C4.395.62E-60.09RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YNL062C4.308.46E-60.12GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YDL143W4.181.47E-50.00CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR116W4.181.48E-50.00MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YDR182W4.171.50E-50.08CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
YER012W4.102.08E-50.07PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YBR154C4.032.84E-50.28RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YBL105C3.749.11E-50.11PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YDL014W3.631.41E-40.11NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YOR207C3.522.12E-40.01RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL167C14.902.70E-50REV3Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
YHL003C14.703.13E-49LAG1Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
YBR171W9.133.33E-20SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YEL063C8.797.44E-19CAN1Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
YGL045W7.911.27E-15RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
YBL042C7.397.35E-14FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YCR061W_p7.242.32E-13YCR061W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
YHR130C_d7.144.60E-13YHR130C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YFR034C7.068.14E-13PHO4Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability
YOR055W_d6.661.36E-11YOR055W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL056W6.641.53E-11OCA2Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
YLR312W-A6.513.75E-11MRPL15Mitochondrial ribosomal protein of the large subunit
YOR104W6.389.10E-11PIN2Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated
YFR032C_p6.114.84E-10RRT5_pPutative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation
YJR107W_p5.911.68E-9YJR107W_pPutative lipase

GO enrichment analysis for SGTC_3212
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0774.01E-9SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.109589RPP1 & pyrimidine depletion
0.0707.07E-8SGTC_1558st069300 45.6 μMTimTec (Pure natural product library)242080000.0704225
0.0663.45E-7SGTC_31769106582 49.5 μMChembridge (Drug-like library)170180270.152778
0.0656.13E-7SGTC_32719137798 49.5 μMChembridge (Drug-like library)64983260.75
0.0631.22E-6SGTC_1320417-1715 25.8 μMChemDiv (Drug-like library)67690980.125RPP1 & pyrimidine depletion
0.0621.74E-6SGTC_33389144300 39.6 μMChembridge (Drug-like library)49012740.0941176Golgi
0.0621.91E-6SGTC_28287998861 71.4 μMChembridge (Drug-like library)29881880.075
0.0622.16E-6SGTC_30729116419 49.5 μMChembridge (Drug-like library)171701620.166667ERG2
0.0603.35E-6SGTC_14863970-0793 4.5 μMChemDiv (Drug-like library)28443300.123077
0.0595.22E-6SGTC_21785812956 200.0 μMChembridge (Fragment library)54033830.112676
0.0587.30E-6SGTC_6583253-0951 102.0 μMChemDiv (Drug-like library)11848780.169014calcium & mitochondrial duress
0.0587.80E-6SGTC_7014424-0219 185.0 μMChemDiv (Drug-like library)29117790.0808081calcium & mitochondrial duress
0.0571.04E-5SGTC_466geldanamycin 89.2 μMICCB bioactive library52883820.0740741RPP1 & pyrimidine depletion
0.0571.25E-5SGTC_2236dapsone 200.0 μMMiscellaneous29550.109091
0.0571.38E-5SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.148649

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3271913779849.47 μM0.756498326Chembridge (Drug-like library)299.370842.94824
SGTC_3264913744249.47 μM0.58928617015726Chembridge (Drug-like library)299.370842.94824
SGTC_3225913081949.47 μM0.4745764547121Chembridge (Drug-like library)293.32322.66624amide catabolism
SGTC_3228913179949.47 μM0.45901617015818Chembridge (Drug-like library)307.349783.15224amide catabolism
SGTC_3274913829849.47 μM0.42372917015756Chembridge (Drug-like library)293.32322.66624
SGTC_3245913482149.47 μM0.40322617014901Chembridge (Drug-like library)307.349783.12224
SGTC_3270913756349.47 μM0.35483917015724Chembridge (Drug-like library)293.32322.66624RPP1 & pyrimidine depletion
SGTC_3355915105844.05 μM0.33333317015728Chembridge (Drug-like library)311.3136632.87125RNA processing & uracil transport
SGTC_3065911388849.47 μM0.32835816648368Chembridge (Drug-like library)338.360522.91515
SGTC_3168910341949.47 μM0.32835816649256Chembridge (Drug-like library)308.334542.9311460S ribosome export