9129884

3-(4-fluorophenyl)-2-(methoxymethyl)-8,9-dihydro-7H-pyrazolo[1,5-a]quinazolin-6-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3213
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17190742
SMILES COCC1=NN2C3=C(C=NC2=C1C4=CC=C(C=C4)F)C(=O)CCC3
Standardized SMILES COCc1nn2c3CCCC(=O)c3cnc2c1c4ccc(F)cc4
Molecular weight 325.3369
ALogP 2.37
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.97
% growth inhibition (Hom. pool) 3.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17190742
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:-3.22|P-value:6.50E-4|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC42(YLR229C)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:CDC43(YGL155W)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:DRS1(YLL008W)|FD-Score:4|P-value:3.12E-5|Clearance:0.28||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG11(YHR007C)|FD-Score:-4.83|P-value:6.89E-7|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:KEG1(YFR042W)|FD-Score:-4.43|P-value:4.67E-6|Clearance:0||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:LSG1(YGL099W)|FD-Score:3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MED8(YBR193C)|FD-Score:4.01|P-value:3.05E-5|Clearance:0.28||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MOT1(YPL082C)|FD-Score:-4.18|P-value:1.46E-5|Clearance:0||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NMD3(YHR170W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.08||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUP85(YJR042W)|FD-Score:-3.77|P-value:8.29E-5|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PRE3(YJL001W)|FD-Score:-6.8|P-value:5.16E-12|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PRP3(YDR473C)|FD-Score:3.87|P-value:5.41E-5|Clearance:0.28||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RFC1(YOR217W)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.28||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPO31(YOR116C)|FD-Score:-3.8|P-value:7.20E-5|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RRP42(YDL111C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SEC63(YOR254C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.07||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:TFA2(YKR062W)|FD-Score:-3.43|P-value:2.98E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TFC3(YAL001C)|FD-Score:-6.18|P-value:3.27E-10|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRS31(YDR472W)|FD-Score:3.77|P-value:8.23E-5|Clearance:0.28||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TSC10(YBR265W)|FD-Score:3.11|P-value:9.32E-4|Clearance:0.1||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:UFE1(YOR075W)|FD-Score:-5.4|P-value:3.33E-8|Clearance:0||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:YDR187C(YDR187C_d)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR073C(YGR073C_d)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:CDC19(YAL038W)|FD-Score:-3.22|P-value:6.50E-4|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC42(YLR229C)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:CDC43(YGL155W)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:DRS1(YLL008W)|FD-Score:4|P-value:3.12E-5|Clearance:0.28||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG11(YHR007C)|FD-Score:-4.83|P-value:6.89E-7|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:KEG1(YFR042W)|FD-Score:-4.43|P-value:4.67E-6|Clearance:0||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:LSG1(YGL099W)|FD-Score:3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MED8(YBR193C)|FD-Score:4.01|P-value:3.05E-5|Clearance:0.28||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MOT1(YPL082C)|FD-Score:-4.18|P-value:1.46E-5|Clearance:0||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NMD3(YHR170W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.08||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUP85(YJR042W)|FD-Score:-3.77|P-value:8.29E-5|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PRE3(YJL001W)|FD-Score:-6.8|P-value:5.16E-12|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PRP3(YDR473C)|FD-Score:3.87|P-value:5.41E-5|Clearance:0.28||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RFC1(YOR217W)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.28||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPO31(YOR116C)|FD-Score:-3.8|P-value:7.20E-5|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RRP42(YDL111C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SEC63(YOR254C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.07||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:TFA2(YKR062W)|FD-Score:-3.43|P-value:2.98E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TFC3(YAL001C)|FD-Score:-6.18|P-value:3.27E-10|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TRS31(YDR472W)|FD-Score:3.77|P-value:8.23E-5|Clearance:0.28||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TSC10(YBR265W)|FD-Score:3.11|P-value:9.32E-4|Clearance:0.1||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:UFE1(YOR075W)|FD-Score:-5.4|P-value:3.33E-8|Clearance:0||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:YDR187C(YDR187C_d)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR073C(YGR073C_d)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17190742
Download HOP data (tab-delimited text)  (excel)
Gene:AIM18(YHR198C)|FD-Score:6.11|P-value:4.83E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG5(YPL227C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARL3(YPL051W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG29(YPL166W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATO2(YNR002C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:AUA1(YFL010W-A)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BNA2(YJR078W)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:CAJ1(YER048C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CYS3(YAL012W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL5(YJR152W)|FD-Score:-3.8|P-value:7.19E-5||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DAL7(YIR031C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:EFT2(YDR385W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:EGD1(YPL037C)|FD-Score:8.04|P-value:4.60E-16||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:FBP1(YLR377C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FET5(YFL041W)|FD-Score:-6.32|P-value:1.27E-10||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:FUB1(YCR076C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FYV10(YIL097W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GIM5(YML094W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GOT1(YMR292W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GSY2(YLR258W)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GTR2(YGR163W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HCH1(YNL281W)|FD-Score:5.05|P-value:2.24E-7||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HUB1(YNR032C-A)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:ICS2(YBR157C)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IMA1(YGR287C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRC14(YOR135C_d)|FD-Score:-4.48|P-value:3.76E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC2(YDR112W_d)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KAP123(YER110C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MGT1(YDL200C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MRE11(YMR224C)|FD-Score:6.39|P-value:8.10E-11||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:NMA111(YNL123W)|FD-Score:-3.83|P-value:6.40E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OCA1(YNL099C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OTU2(YHL013C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PAH1(YMR165C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PSY3(YLR376C)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RAD9(YDR217C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RDL1(YOR285W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:REG1(YDR028C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPL36A(YMR194W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT107(YHR154W)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SLX5(YDL013W)|FD-Score:4.54|P-value:2.79E-6||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SOL4(YGR248W)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SPO14(YKR031C)|FD-Score:-6.86|P-value:3.48E-12||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPS18(YNL204C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPS4(YOR313C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SSA3(YBL075C)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSA4(YER103W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:STP2(YHR006W)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TFB6(YOR352W)|FD-Score:-4.89|P-value:4.93E-7||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TIR4(YOR009W)|FD-Score:5.13|P-value:1.42E-7||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:TOS2(YGR221C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TWF1(YGR080W)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly Gene:UBP7(YIL156W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:UGA2(YBR006W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URN1(YPR152C)|FD-Score:4.82|P-value:7.33E-7||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:YAF9(YNL107W)|FD-Score:-5.03|P-value:2.39E-7||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YAR1(YPL239W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBL095W(YBL095W_p)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR061W(YCR061W_p)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YER034W(YER034W_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER053C-A(YER053C-A_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YHR177W(YHR177W_p)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YJR018W(YJR018W_d)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YLR050C(YLR050C_p)|FD-Score:-5.41|P-value:3.08E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YLR211C(YLR211C_p)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR236C(YLR236C_d)|FD-Score:7.87|P-value:1.78E-15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR196W(YMR196W_p)|FD-Score:-5.14|P-value:1.38E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL022C(YNL022C_p)|FD-Score:-7.05|P-value:8.76E-13||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YOR053W(YOR053W_d)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR072W(YOR072W_d)|FD-Score:4.42|P-value:4.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPR126C(YPR126C_d)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZIP1(YDR285W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:AIM18(YHR198C)|FD-Score:6.11|P-value:4.83E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG5(YPL227C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARL3(YPL051W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG29(YPL166W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATO2(YNR002C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:AUA1(YFL010W-A)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BNA2(YJR078W)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:CAJ1(YER048C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CYS3(YAL012W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL5(YJR152W)|FD-Score:-3.8|P-value:7.19E-5||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DAL7(YIR031C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:EFT2(YDR385W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:EGD1(YPL037C)|FD-Score:8.04|P-value:4.60E-16||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:FBP1(YLR377C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FET5(YFL041W)|FD-Score:-6.32|P-value:1.27E-10||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:FUB1(YCR076C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FYV10(YIL097W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GIM5(YML094W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GOT1(YMR292W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GSY2(YLR258W)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GTR2(YGR163W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HCH1(YNL281W)|FD-Score:5.05|P-value:2.24E-7||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HUB1(YNR032C-A)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:ICS2(YBR157C)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IMA1(YGR287C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRC14(YOR135C_d)|FD-Score:-4.48|P-value:3.76E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC2(YDR112W_d)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KAP123(YER110C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MGT1(YDL200C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MRE11(YMR224C)|FD-Score:6.39|P-value:8.10E-11||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:NMA111(YNL123W)|FD-Score:-3.83|P-value:6.40E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OCA1(YNL099C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OTU2(YHL013C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PAH1(YMR165C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PSY3(YLR376C)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RAD9(YDR217C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RDL1(YOR285W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:REG1(YDR028C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPL36A(YMR194W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT107(YHR154W)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SLX5(YDL013W)|FD-Score:4.54|P-value:2.79E-6||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SOL4(YGR248W)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SPO14(YKR031C)|FD-Score:-6.86|P-value:3.48E-12||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPS18(YNL204C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPS4(YOR313C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SSA3(YBL075C)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSA4(YER103W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:STP2(YHR006W)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TFB6(YOR352W)|FD-Score:-4.89|P-value:4.93E-7||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TIR4(YOR009W)|FD-Score:5.13|P-value:1.42E-7||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:TOS2(YGR221C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TWF1(YGR080W)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly Gene:UBP7(YIL156W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:UGA2(YBR006W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URN1(YPR152C)|FD-Score:4.82|P-value:7.33E-7||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:YAF9(YNL107W)|FD-Score:-5.03|P-value:2.39E-7||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YAR1(YPL239W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBL095W(YBL095W_p)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR061W(YCR061W_p)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YER034W(YER034W_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER053C-A(YER053C-A_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YHR177W(YHR177W_p)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YJR018W(YJR018W_d)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YLR050C(YLR050C_p)|FD-Score:-5.41|P-value:3.08E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YLR211C(YLR211C_p)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR236C(YLR236C_d)|FD-Score:7.87|P-value:1.78E-15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR196W(YMR196W_p)|FD-Score:-5.14|P-value:1.38E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL022C(YNL022C_p)|FD-Score:-7.05|P-value:8.76E-13||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YOR053W(YOR053W_d)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR072W(YOR072W_d)|FD-Score:4.42|P-value:4.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPR126C(YPR126C_d)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZIP1(YDR285W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR217W4.241.13E-50.28RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YBR193C4.013.05E-50.28MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YLL008W4.003.12E-50.28DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
YDR473C3.875.41E-50.28PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YDR472W3.778.23E-50.28TRS31One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YGL099W3.492.42E-45.32E-4LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDL111C3.492.43E-40.04RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YGR073C_d3.452.82E-40.05YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YOR254C3.393.45E-40.07SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YHR170W3.324.45E-40.08NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDR187C_d3.245.92E-40.13YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YBR265W3.119.32E-40.10TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YJR064W3.010.001300.00CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGR114C_d3.010.001320.08YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YMR134W_p2.930.001700.02ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL037C8.044.60E-16EGD1Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b
YLR236C_d7.871.78E-15YLR236C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR224C6.398.10E-11MRE11Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
YHR198C6.114.83E-10AIM18Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YOR009W5.131.42E-7TIR4Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock
YNL281W5.052.24E-7HCH1Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
YGR163W5.002.81E-7GTR2Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
YPR152C4.827.33E-7URN1Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase
YGR248W4.731.14E-6SOL46-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
YBR069C4.671.48E-6TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YER053C-A_p4.641.77E-6YER053C-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YNL099C4.582.33E-6OCA1Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
YDL013W4.542.79E-6SLX5Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers
YFL010W-A4.513.31E-6AUA1Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease
YOR072W_d4.424.83E-6YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive

GO enrichment analysis for SGTC_3213
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0912.34E-12SGTC_24245537928 59.2 μMMiscellaneous53828020.0609756
0.0899.38E-12SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.071428660S ribosome export
0.0765.41E-9SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.068965560S ribosome export
0.0756.99E-9SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.10975660S ribosome export
0.0722.47E-8SGTC_31019120531 49.5 μMChembridge (Drug-like library)248249040.148148
0.0722.79E-8SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.075949460S ribosome export
0.0715.37E-8SGTC_1983454-2873 16.2 μMChemDiv (Drug-like library)7109570.090909160S ribosome export
0.0706.36E-8SGTC_4253825-7017 13.1 μMChemDiv (Drug-like library)33974400.139241
0.0708.71E-8SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.09195460S ribosome export
0.0691.15E-7SGTC_1360097-0015 404.5 μMChemDiv (Drug-like library)48613370.069444460S ribosome export
0.0663.39E-7SGTC_1613st002381 52.1 μMTimTec (Natural product derivative library)5511670.101124
0.0664.17E-7SGTC_32149129694 49.5 μMChembridge (Drug-like library)171586550.0952381
0.0655.62E-7SGTC_1969st069479 40.1 μMTimTec (Natural product derivative library)38954850.134615
0.0649.97E-7SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.080808160S ribosome export
0.0631.22E-6SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.064935160S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1086cerivastatin2.18 μM0.24705923663992NIH Clinical Collection481.5320922.7327azole & statin
SGTC_3097911724649.47 μM0.24390216423892Chembridge (Drug-like library)381.4266432.37917
SGTC_3231913231349.47 μM0.2352944905967Chembridge (Drug-like library)251.326283.13602
SGTC_2996906619271.43 μM0.2162166472854Chembridge (Drug-like library)326.3481433.41906
SGTC_3244913448949.47 μM0.212516584271Chembridge (Drug-like library)340.806783.51604
SGTC_3321913779326.83 μM0.2073174904192Chembridge (Drug-like library)343.424943.63414cell wall
SGTC_13722752-0194270 μM0.205128823334ChemDiv (Drug-like library)296.36362.29712
SGTC_3235913260249.47 μM0.2051284912240Chembridge (Drug-like library)352.430163.31605
SGTC_13863228-009533.8 μM0.202532870181ChemDiv (Drug-like library)341.405764.73903
SGTC_3249913518349.47 μM0.24902779Chembridge (Drug-like library)365.471983.0271560S ribosome export