9129942

N-(2-ethylphenyl)-4-methyl-2-pyridin-3-yl-1,3-thiazole-5-carboxamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3215
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17682944
SMILES CCC1=CC=CC=C1NC(=O)C2=C(N=C(S2)C3=CN=CC=C3)C
Standardized SMILES CCc1ccccc1NC(=O)c2sc(nc2C)c3cccnc3
Molecular weight 323.4121
ALogP 3.36
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.88
% growth inhibition (Hom. pool) 4.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17682944
Download HIP data (tab-delimited text)  (excel)
Gene:CDC16(YKL022C)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.16||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:DAD1(YDR016C)|FD-Score:-3.49|P-value:2.37E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DUO1(YGL061C)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.05||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERO1(YML130C)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.01||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:FCF1(YDR339C)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.02||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:GPI11(YDR302W)|FD-Score:4.84|P-value:6.38E-7|Clearance:0.57||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:MOT1(YPL082C)|FD-Score:3.24|P-value:5.94E-4|Clearance:0.07||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NAB3(YPL190C)|FD-Score:-3.34|P-value:4.12E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NUS1(YDL193W)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.01||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:POP4(YBR257W)|FD-Score:-4.95|P-value:3.71E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRE5(YMR314W)|FD-Score:3.15|P-value:8.26E-4|Clearance:0.02||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RET1(YOR207C)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.02||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC25(YKL144C)|FD-Score:-3.88|P-value:5.22E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPC40(YPR110C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPL5(YPL131W)|FD-Score:3.79|P-value:7.52E-5|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPN8(YOR261C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPS31(YLR167W)|FD-Score:4.28|P-value:9.46E-6|Clearance:0.02||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP14(YKL082C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.21||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:SNU71(YGR013W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.03||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:TAO3(YIL129C)|FD-Score:5.69|P-value:6.30E-9|Clearance:0.85||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:TFB3(YDR460W)|FD-Score:-3.33|P-value:4.30E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:UTP23(YOR004W)|FD-Score:4.26|P-value:1.02E-5|Clearance:0.47||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:YPL251W(YPL251W_d)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:CDC16(YKL022C)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.16||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:DAD1(YDR016C)|FD-Score:-3.49|P-value:2.37E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DUO1(YGL061C)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.05||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERO1(YML130C)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.01||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:FCF1(YDR339C)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.02||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:GPI11(YDR302W)|FD-Score:4.84|P-value:6.38E-7|Clearance:0.57||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:MOT1(YPL082C)|FD-Score:3.24|P-value:5.94E-4|Clearance:0.07||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NAB3(YPL190C)|FD-Score:-3.34|P-value:4.12E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NUS1(YDL193W)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.01||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:POP4(YBR257W)|FD-Score:-4.95|P-value:3.71E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRE5(YMR314W)|FD-Score:3.15|P-value:8.26E-4|Clearance:0.02||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RET1(YOR207C)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.02||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC25(YKL144C)|FD-Score:-3.88|P-value:5.22E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPC40(YPR110C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPL5(YPL131W)|FD-Score:3.79|P-value:7.52E-5|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPN8(YOR261C)|FD-Score:3.39|P-value:3.53E-4|Clearance:0.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPS31(YLR167W)|FD-Score:4.28|P-value:9.46E-6|Clearance:0.02||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP14(YKL082C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.21||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:SNU71(YGR013W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.03||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:TAO3(YIL129C)|FD-Score:5.69|P-value:6.30E-9|Clearance:0.85||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:TFB3(YDR460W)|FD-Score:-3.33|P-value:4.30E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:UTP23(YOR004W)|FD-Score:4.26|P-value:1.02E-5|Clearance:0.47||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:YPL251W(YPL251W_d)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17682944
Download HOP data (tab-delimited text)  (excel)
Gene:ADK2(YER170W)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AIM6(YDL237W)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:APS3(YJL024C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARR1(YPR199C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATP19(YOL077W-A)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:COA3(YJL062W-A)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:CSF1(YLR087C)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CYS4(YGR155W)|FD-Score:-3.76|P-value:8.43E-5||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAP2(YHR028C)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DOT6(YER088C)|FD-Score:-4.36|P-value:6.48E-6||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DPP1(YDR284C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:ERG3(YLR056W)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FUR4(YBR021W)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN20(YFR009W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN5(YGR252W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GOT1(YMR292W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:IME4(YGL192W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:KES1(YPL145C)|FD-Score:4.92|P-value:4.29E-7||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LSC2(YGR244C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MCM21(YDR318W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MDM34(YGL219C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MGT1(YDL200C)|FD-Score:-6.91|P-value:2.39E-12||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MIC14(YDR031W)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRPL8(YJL063C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS2(YDL107W)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:PCL5(YHR071W)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PHO4(YFR034C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:RIB4(YOL143C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RPN10(YHR200W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPS17A(YML024W)|FD-Score:-3.76|P-value:8.61E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RTT101(YJL047C)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAM3(YPL274W)|FD-Score:-3.95|P-value:3.88E-5||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SGM1(YJR134C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLM2(YNL047C)|FD-Score:-6.29|P-value:1.63E-10||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication Gene:SOL4(YGR248W)|FD-Score:5.06|P-value:2.13E-7||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:TDA11(YHR159W_p)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:THO1(YER063W)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation Gene:TOS4(YLR183C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication Gene:TUF1(YOR187W)|FD-Score:-3.8|P-value:7.27E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TUL1(YKL034W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBA3(YPR066W)|FD-Score:-3.74|P-value:9.27E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP9(YER098W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:UFD2(YDL190C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VMA11(YPL234C)|FD-Score:-5.07|P-value:2.02E-7||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:YBR219C(YBR219C_p)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YCR050C(YCR050C_p)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDR095C(YDR095C_d)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL109W(YGL109W_d)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YGL262W(YGL262W_p)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YLR152C(YLR152C_p)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR283W(YLR283W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YLR407W(YLR407W_p)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YMR196W(YMR196W_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL057W(YNL057W_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR105W(YOR105W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR318C(YOR318C_d)|FD-Score:4.38|P-value:5.94E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR385W(YOR385W_p)|FD-Score:4.34|P-value:7.21E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:ZIP1(YDR285W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ADK2(YER170W)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AIM6(YDL237W)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:APS3(YJL024C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARR1(YPR199C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATP19(YOL077W-A)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:COA3(YJL062W-A)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:CSF1(YLR087C)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CYS4(YGR155W)|FD-Score:-3.76|P-value:8.43E-5||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAP2(YHR028C)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DOT6(YER088C)|FD-Score:-4.36|P-value:6.48E-6||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DPP1(YDR284C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:ERG3(YLR056W)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FUR4(YBR021W)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN20(YFR009W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN5(YGR252W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GOT1(YMR292W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:IME4(YGL192W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:KES1(YPL145C)|FD-Score:4.92|P-value:4.29E-7||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LSC2(YGR244C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MCM21(YDR318W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MDM34(YGL219C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MGT1(YDL200C)|FD-Score:-6.91|P-value:2.39E-12||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MIC14(YDR031W)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRPL8(YJL063C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS2(YDL107W)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:PCL5(YHR071W)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PHO4(YFR034C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:RIB4(YOL143C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin Gene:RPN10(YHR200W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPS17A(YML024W)|FD-Score:-3.76|P-value:8.61E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RRT5(YFR032C_p)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RTT101(YJL047C)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAM3(YPL274W)|FD-Score:-3.95|P-value:3.88E-5||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SGM1(YJR134C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLM2(YNL047C)|FD-Score:-6.29|P-value:1.63E-10||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication Gene:SOL4(YGR248W)|FD-Score:5.06|P-value:2.13E-7||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:TDA11(YHR159W_p)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:THO1(YER063W)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation Gene:TOS4(YLR183C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication Gene:TUF1(YOR187W)|FD-Score:-3.8|P-value:7.27E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TUL1(YKL034W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBA3(YPR066W)|FD-Score:-3.74|P-value:9.27E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP9(YER098W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:UFD2(YDL190C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VMA11(YPL234C)|FD-Score:-5.07|P-value:2.02E-7||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:YBR219C(YBR219C_p)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YCR050C(YCR050C_p)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDR095C(YDR095C_d)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL109W(YGL109W_d)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YGL262W(YGL262W_p)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YLR152C(YLR152C_p)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR283W(YLR283W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YLR407W(YLR407W_p)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YMR196W(YMR196W_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL057W(YNL057W_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR105W(YOR105W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR318C(YOR318C_d)|FD-Score:4.38|P-value:5.94E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR385W(YOR385W_p)|FD-Score:4.34|P-value:7.21E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:ZIP1(YDR285W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL129C5.696.30E-90.85TAO3Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
YDR302W4.846.38E-70.57GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YLR167W4.289.46E-60.02RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YOR004W4.261.02E-50.47UTP23Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
YPL131W3.797.52E-50.14RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YKL082C3.651.32E-40.21RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YGL061C3.442.91E-40.05DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YOR261C3.393.53E-40.06RPN8Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
YDL193W3.334.42E-40.01NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YOR207C3.324.56E-40.02RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDR339C3.304.87E-40.02FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YML130C3.285.20E-40.01ERO1Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
YGR013W3.275.37E-40.03SNU71Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
YPL082C3.245.94E-40.07MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YPL251W_d3.177.53E-40.03YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR248W5.062.13E-7SOL46-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
YBR021W5.012.76E-7FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YHR071W4.963.58E-7PCL5Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity
YPL145C4.924.29E-7KES1One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
YLR152C_p4.552.64E-6YLR152C_pPutative protein of unknown function; YLR152C is not an essential gene
YOR318C_d4.385.94E-6YOR318C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
YOR385W_p4.347.21E-6YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YGL109W_d4.279.81E-6YGL109W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C
YGL219C4.151.68E-5MDM34Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth
YDR318W4.121.86E-5MCM21Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
YER063W3.816.84E-5THO1Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation
YMR292W3.681.15E-4GOT1Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition
YOL077W-A3.631.42E-4ATP19Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase
YKL034W3.631.43E-4TUL1Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1
YLR407W_p3.581.75E-4YLR407W_pPutative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus

GO enrichment analysis for SGTC_3215
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0707.49E-8SGTC_32149129694 49.5 μMChembridge (Drug-like library)171586550.097561
0.0699.80E-8SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.0632911RNA processing & uracil transport
0.0681.55E-7SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.112676RNA processing & uracil transport
0.0656.44E-7SGTC_32649137442 49.5 μMChembridge (Drug-like library)170157260.166667
0.0612.76E-6SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.0972222RNA processing & uracil transport
0.0595.20E-6SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0952381RPP1 & pyrimidine depletion
0.0552.22E-5SGTC_452diindolylmethane 8.0 μMICCB bioactive library30710.114754RNA processing & uracil transport
0.0552.54E-5SGTC_23097664088 163.9 μMChembridge (Fragment library)7049220.0869565
0.0543.31E-5SGTC_33559151058 44.0 μMChembridge (Drug-like library)170157280.184211RNA processing & uracil transport
0.0526.50E-5SGTC_21455634607 200.0 μMChembridge (Fragment library)3086620.112676
0.0501.06E-4SGTC_1871488-2104 32.7 μMChemDiv (Drug-like library)28528590.130435calcium & mitochondrial duress
0.0491.56E-4SGTC_23057443522 69.2 μMChembridge (Fragment library)54158860.0769231
0.0482.04E-4SGTC_5321548-0774 215.0 μMChemDiv (Drug-like library)59329930.137931
0.0482.55E-4SGTC_22927671323 168.1 μMChembridge (Fragment library)9375040.135135RNA processing & uracil transport
0.0454.81E-4SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.078125RNA pol III & RNase P/MRP

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3233913234049.47 μM0.73584917682950Chembridge (Drug-like library)329.803983.08314
SGTC_3251913504749.47 μM0.70909117682963Chembridge (Drug-like library)325.38492.4021560S ribosome export
SGTC_3337914434233.55 μM0.62517682949Chembridge (Drug-like library)313.3493832.62415
SGTC_3269913782349.47 μM0.61403517682936Chembridge (Drug-like library)309.38552.9041460S ribosome export
SGTC_3229913220449.47 μM0.51515217682924Chembridge (Drug-like library)327.443843.16914
SGTC_21545570129200 μM0.509091673366Chembridge (Fragment library)227.261821.68413
SGTC_3090911769649.47 μM0.3833337923821Chembridge (Drug-like library)297.2475534.37915
SGTC_3245913482149.47 μM0.36923117014901Chembridge (Drug-like library)307.349783.12224
SGTC_2885905773251.95 μM0.3593756471414Chembridge (Drug-like library)297.414564.71512
SGTC_14964469-071357.4 μM0.338235746691ChemDiv (Drug-like library)297.37483.50615