9129716

methyl 2-(morpholine-4-carbonylamino)-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carboxylate

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3216
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 8056934
SMILES COC(=O)C1=C(SC2=C1CCC2)NC(=O)N3CCOCC3
Standardized SMILES COC(=O)c1c2CCCc2sc1NC(=O)N3CCOCC3
Molecular weight 310.3687
ALogP 1.41
H-bond donor count 1
H-bond acceptor count 5
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.88
% growth inhibition (Hom. pool) 4.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 8056934
Download HIP data (tab-delimited text)  (excel)
Gene:AME1(YBR211C)|FD-Score:-3.13|P-value:8.64E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:DIP2(YLR129W)|FD-Score:-3.96|P-value:3.74E-5|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:KEG1(YFR042W)|FD-Score:-7.75|P-value:4.61E-15|Clearance:0||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MEX67(YPL169C)|FD-Score:-3.52|P-value:2.17E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NUP85(YJR042W)|FD-Score:-4.28|P-value:9.24E-6|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:NUS1(YDL193W)|FD-Score:4.45|P-value:4.24E-6|Clearance:0.27||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PKC1(YBL105C)|FD-Score:7.22|P-value:2.54E-13|Clearance:1.28||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE7(YBL041W)|FD-Score:-3.54|P-value:1.96E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRP43(YGL120C)|FD-Score:-3.17|P-value:7.64E-4|Clearance:0||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RPL5(YPL131W)|FD-Score:5.61|P-value:9.86E-9|Clearance:0.68||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:5.94|P-value:1.42E-9|Clearance:0.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT3(YDR394W)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RSC9(YML127W)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.89||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SKI6(YGR195W)|FD-Score:-3.52|P-value:2.12E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SPP381(YBR152W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.15||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TFB4(YPR056W)|FD-Score:-3.33|P-value:4.34E-4|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TFC4(YGR047C)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.05||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:YGR114C(YGR114C_d)|FD-Score:4.93|P-value:4.03E-7|Clearance:0.39||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YKL111C(YKL111C_d)|FD-Score:3.29|P-value:4.97E-4|Clearance:0.05||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:YOR282W(YOR282W_d)|FD-Score:4.54|P-value:2.76E-6|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:YPP1(YGR198W)|FD-Score:3.2|P-value:6.97E-4|Clearance:0.09||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:AME1(YBR211C)|FD-Score:-3.13|P-value:8.64E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:DIP2(YLR129W)|FD-Score:-3.96|P-value:3.74E-5|Clearance:0||SGD DESC:Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex Gene:KEG1(YFR042W)|FD-Score:-7.75|P-value:4.61E-15|Clearance:0||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MEX67(YPL169C)|FD-Score:-3.52|P-value:2.17E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NUP85(YJR042W)|FD-Score:-4.28|P-value:9.24E-6|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:NUS1(YDL193W)|FD-Score:4.45|P-value:4.24E-6|Clearance:0.27||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PKC1(YBL105C)|FD-Score:7.22|P-value:2.54E-13|Clearance:1.28||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE7(YBL041W)|FD-Score:-3.54|P-value:1.96E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRP43(YGL120C)|FD-Score:-3.17|P-value:7.64E-4|Clearance:0||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RPL5(YPL131W)|FD-Score:5.61|P-value:9.86E-9|Clearance:0.68||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:5.94|P-value:1.42E-9|Clearance:0.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT3(YDR394W)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RSC9(YML127W)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.89||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SKI6(YGR195W)|FD-Score:-3.52|P-value:2.12E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SPP381(YBR152W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.15||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TFB4(YPR056W)|FD-Score:-3.33|P-value:4.34E-4|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TFC4(YGR047C)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.05||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:YGR114C(YGR114C_d)|FD-Score:4.93|P-value:4.03E-7|Clearance:0.39||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YKL111C(YKL111C_d)|FD-Score:3.29|P-value:4.97E-4|Clearance:0.05||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:YOR282W(YOR282W_d)|FD-Score:4.54|P-value:2.76E-6|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:YPP1(YGR198W)|FD-Score:3.2|P-value:6.97E-4|Clearance:0.09||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 8056934
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADE4(YMR300C)|FD-Score:-4|P-value:3.16E-5||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AHC2(YCR082W)|FD-Score:-3.72|P-value:9.86E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM25(YJR100C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALD4(YOR374W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:ALT1(YLR089C)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ANT1(YPR128C)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APL5(YPL195W)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARN2(YHL047C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO1(YDR127W)|FD-Score:-5.75|P-value:4.50E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARR1(YPR199C)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ASF2(YDL197C)|FD-Score:-4.98|P-value:3.22E-7||SGD DESC:Anti-silencing protein that causes derepression of silent loci when overexpressed Gene:ASG1(YIL130W)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:BCH2(YKR027W)|FD-Score:-4.88|P-value:5.40E-7||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BFR1(YOR198C)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:COS1(YNL336W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CTR1(YPR124W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL5(YJR152W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DUF1(YOL087C)|FD-Score:8.41|P-value:2.01E-17||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM29(YHL030W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ELC1(YPL046C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:EOS1(YNL080C)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FET5(YFL041W)|FD-Score:-5.92|P-value:1.58E-9||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:FKH2(YNL068C)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FLD1(YLR404W)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FMP10(YER182W_p)|FD-Score:5.3|P-value:5.83E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP16(YDR070C_p)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FRA1(YLL029W)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FYV8(YGR196C)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCV1(YDR019C)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GDS1(YOR355W)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GET1(YGL020C)|FD-Score:6.23|P-value:2.31E-10||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GLO1(YML004C)|FD-Score:6.64|P-value:1.62E-11||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GPX1(YKL026C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:HAT2(YEL056W)|FD-Score:-5.69|P-value:6.19E-9||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HBN1(YCL026C-B_p)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HEF3(YNL014W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HIS6(YIL020C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HTA2(YBL003C)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:INP52(YNL106C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain Gene:IRC6(YFR043C)|FD-Score:4.84|P-value:6.49E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IST3(YIR005W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:KTI11(YBL071W-A)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MCM22(YJR135C)|FD-Score:-5.14|P-value:1.38E-7||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MCT1(YOR221C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM34(YGL219C)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MGT1(YDL200C)|FD-Score:-6.89|P-value:2.75E-12||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MNE1(YOR350C)|FD-Score:-4.99|P-value:2.95E-7||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MNN1(YER001W)|FD-Score:-4|P-value:3.15E-5||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MON2(YNL297C)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRK1(YDL079C)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRP10(YDL045W-A)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPS17(YMR188C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS2(YDL107W)|FD-Score:3.24|P-value:5.87E-4||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:NCS2(YNL119W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NMA111(YNL123W)|FD-Score:5.35|P-value:4.38E-8||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OPT2(YPR194C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:ORM1(YGR038W)|FD-Score:-4.52|P-value:3.15E-6||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OXA1(YER154W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEX2(YJL210W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PEX34(YCL056C)|FD-Score:-6.35|P-value:1.06E-10||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PIF1(YML061C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PRY1(YJL079C)|FD-Score:4.27|P-value:9.57E-6||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RAD7(YJR052W)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RDI1(YDL135C)|FD-Score:-3.74|P-value:9.34E-5||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:RDL2(YOR286W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RIM9(YMR063W)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL22A(YLR061W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL39(YJL189W)|FD-Score:-3.76|P-value:8.44E-5||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RPP2B(YDR382W)|FD-Score:-10.4|P-value:1.92E-25||SGD DESC:Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RSM25(YIL093C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC2(YBR147W)|FD-Score:-4.5|P-value:3.46E-6||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:SAG1(YJR004C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SAM37(YMR060C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SDC1(YDR469W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SER2(YGR208W)|FD-Score:-3.87|P-value:5.51E-5||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SFC1(YJR095W)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SKM1(YOL113W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLP1(YOR154W_p)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SOL2(YCR073W-A)|FD-Score:7.13|P-value:4.95E-13||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPR3(YGR059W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SRB2(YHR041C)|FD-Score:-6.2|P-value:2.89E-10||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRO9(YCL037C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRP40(YKR092C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:STB2(YMR053C)|FD-Score:6.5|P-value:4.11E-11||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SWR1(YDR334W)|FD-Score:4.39|P-value:5.76E-6||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT2(YOL020W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:THO1(YER063W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation Gene:TRM3(YDL112W)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TSR2(YLR435W)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TVP18(YMR071C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMA11(YPL234C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS29(YHR012W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS5(YOR069W)|FD-Score:5.62|P-value:9.79E-9||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YAL065C(YAL065C_p)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-3.88|P-value:5.19E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR051W(YBR051W_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR056W(YBR056W_p)|FD-Score:-3.83|P-value:6.45E-5||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR174C(YBR174C_d)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR300C(YBR300C_d)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDR286C(YDR286C_p)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YER053C-A(YER053C-A_p)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER084W(YER084W_p)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YER130C(YER130C_p)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFR039C(YFR039C_p)|FD-Score:-4.23|P-value:1.18E-5||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL041C-B(YGL041C-B_p)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR069W(YGR069W_d)|FD-Score:-11.9|P-value:4.58E-33||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR291C(YGR291C_d)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL213W(YJL213W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YLL044W(YLL044W_d)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR177W(YLR177W_p)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR211C(YLR211C_p)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR236C(YLR236C_d)|FD-Score:6.37|P-value:9.42E-11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR285C-A(YLR285C-A_p)|FD-Score:-4.5|P-value:3.35E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR407W(YLR407W_p)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YMR196W(YMR196W_p)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNL022C(YNL022C_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNL193W(YNL193W_p)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YPL272C(YPL272C_p)|FD-Score:4.78|P-value:8.97E-7||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:ACE2(YLR131C)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADE4(YMR300C)|FD-Score:-4|P-value:3.16E-5||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AHC2(YCR082W)|FD-Score:-3.72|P-value:9.86E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM25(YJR100C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALD4(YOR374W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:ALT1(YLR089C)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:ANT1(YPR128C)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APL5(YPL195W)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARN2(YHL047C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO1(YDR127W)|FD-Score:-5.75|P-value:4.50E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARR1(YPR199C)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ASF2(YDL197C)|FD-Score:-4.98|P-value:3.22E-7||SGD DESC:Anti-silencing protein that causes derepression of silent loci when overexpressed Gene:ASG1(YIL130W)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:BCH2(YKR027W)|FD-Score:-4.88|P-value:5.40E-7||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BFR1(YOR198C)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:COS1(YNL336W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CTR1(YPR124W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL5(YJR152W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DUF1(YOL087C)|FD-Score:8.41|P-value:2.01E-17||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM29(YHL030W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ELC1(YPL046C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:EOS1(YNL080C)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FET5(YFL041W)|FD-Score:-5.92|P-value:1.58E-9||SGD DESC:Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport Gene:FKH2(YNL068C)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FLD1(YLR404W)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FMP10(YER182W_p)|FD-Score:5.3|P-value:5.83E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP16(YDR070C_p)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FRA1(YLL029W)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FYV8(YGR196C)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCV1(YDR019C)|FD-Score:-4.75|P-value:1.03E-6||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GDS1(YOR355W)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GET1(YGL020C)|FD-Score:6.23|P-value:2.31E-10||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GLO1(YML004C)|FD-Score:6.64|P-value:1.62E-11||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GPX1(YKL026C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:HAT2(YEL056W)|FD-Score:-5.69|P-value:6.19E-9||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HBN1(YCL026C-B_p)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HEF3(YNL014W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HIS6(YIL020C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HTA2(YBL003C)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:INP52(YNL106C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain Gene:IRC6(YFR043C)|FD-Score:4.84|P-value:6.49E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IST3(YIR005W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:KTI11(YBL071W-A)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MCM22(YJR135C)|FD-Score:-5.14|P-value:1.38E-7||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MCT1(YOR221C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM34(YGL219C)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MGT1(YDL200C)|FD-Score:-6.89|P-value:2.75E-12||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MNE1(YOR350C)|FD-Score:-4.99|P-value:2.95E-7||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MNN1(YER001W)|FD-Score:-4|P-value:3.15E-5||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MON2(YNL297C)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRK1(YDL079C)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRP10(YDL045W-A)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPS17(YMR188C)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS2(YDL107W)|FD-Score:3.24|P-value:5.87E-4||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:NCS2(YNL119W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NMA111(YNL123W)|FD-Score:5.35|P-value:4.38E-8||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OPT2(YPR194C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:ORM1(YGR038W)|FD-Score:-4.52|P-value:3.15E-6||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OXA1(YER154W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEX2(YJL210W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PEX34(YCL056C)|FD-Score:-6.35|P-value:1.06E-10||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PIF1(YML061C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PRY1(YJL079C)|FD-Score:4.27|P-value:9.57E-6||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RAD7(YJR052W)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RDI1(YDL135C)|FD-Score:-3.74|P-value:9.34E-5||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:RDL2(YOR286W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RIM9(YMR063W)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL22A(YLR061W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL39(YJL189W)|FD-Score:-3.76|P-value:8.44E-5||SGD DESC:Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog Gene:RPP2B(YDR382W)|FD-Score:-10.4|P-value:1.92E-25||SGD DESC:Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RSM25(YIL093C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC2(YBR147W)|FD-Score:-4.5|P-value:3.46E-6||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:SAG1(YJR004C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SAM37(YMR060C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SDC1(YDR469W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SER2(YGR208W)|FD-Score:-3.87|P-value:5.51E-5||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SFC1(YJR095W)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SKM1(YOL113W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLP1(YOR154W_p)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SOL2(YCR073W-A)|FD-Score:7.13|P-value:4.95E-13||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPR3(YGR059W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SRB2(YHR041C)|FD-Score:-6.2|P-value:2.89E-10||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRO9(YCL037C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRP40(YKR092C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:STB2(YMR053C)|FD-Score:6.5|P-value:4.11E-11||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SWR1(YDR334W)|FD-Score:4.39|P-value:5.76E-6||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT2(YOL020W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:THO1(YER063W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation Gene:TRM3(YDL112W)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TSR2(YLR435W)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TVP18(YMR071C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMA11(YPL234C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS29(YHR012W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS5(YOR069W)|FD-Score:5.62|P-value:9.79E-9||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YAL065C(YAL065C_p)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-3.88|P-value:5.19E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR051W(YBR051W_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR056W(YBR056W_p)|FD-Score:-3.83|P-value:6.45E-5||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR174C(YBR174C_d)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR300C(YBR300C_d)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDR286C(YDR286C_p)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YER053C-A(YER053C-A_p)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER084W(YER084W_p)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YER130C(YER130C_p)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFR039C(YFR039C_p)|FD-Score:-4.23|P-value:1.18E-5||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL041C-B(YGL041C-B_p)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR069W(YGR069W_d)|FD-Score:-11.9|P-value:4.58E-33||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR291C(YGR291C_d)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL213W(YJL213W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YLL044W(YLL044W_d)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR177W(YLR177W_p)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR211C(YLR211C_p)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR236C(YLR236C_d)|FD-Score:6.37|P-value:9.42E-11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR285C-A(YLR285C-A_p)|FD-Score:-4.5|P-value:3.35E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR407W(YLR407W_p)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YMR196W(YMR196W_p)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNL022C(YNL022C_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNL193W(YNL193W_p)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YPL272C(YPL272C_p)|FD-Score:4.78|P-value:8.97E-7||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL105C7.222.54E-131.28PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YHR062C5.941.42E-90.33RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPL131W5.619.86E-90.68RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YGR114C_d4.934.03E-70.39YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YOR282W_d4.542.76E-60.09YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YDL193W4.454.24E-60.27NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YML127W4.181.45E-50.89RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YKL111C_d3.294.97E-40.05YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YGR047C3.245.92E-40.05TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YGR198W3.206.97E-40.09YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YBR152W3.119.49E-40.14SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YBR109C2.960.001540.03CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YGR147C2.930.001710.03NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YBR140C2.890.001900.01IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YGL137W2.880.001960.01SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL087C8.412.01E-17DUF1Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
YCR073W-A7.134.95E-13SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
YML004C6.641.62E-11GLO1Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
YMR053C6.504.11E-11STB2Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p
YLR236C_d6.379.42E-11YLR236C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL020C6.232.31E-10GET1Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
YNL068C5.961.23E-9FKH2Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication
YOR069W5.629.79E-9VPS5Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
YNL123W5.354.38E-8NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YER182W_p5.305.83E-8FMP10_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YLR131C4.963.59E-7ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YFR043C4.846.49E-7IRC6Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci
YPL272C_p4.788.97E-7YPL272C_pPutative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene
YBR300C_d4.711.23E-6YBR300C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene
YOR198C4.602.14E-6BFR1Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity

GO enrichment analysis for SGTC_3216
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0781.67E-9SGTC_11710368-0077 21.4 μMChemDiv (Drug-like library)32397790.084507
0.0715.80E-8SGTC_32249130643 49.5 μMChembridge (Drug-like library)176443950.0810811RPP1 & pyrimidine depletion
0.0691.24E-7SGTC_1115galangin 695.7 nMTimTec (Natural product library)52816160.0410959RPP1 & pyrimidine depletion
0.0672.54E-7SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.147059RPP1 & pyrimidine depletion
0.0663.48E-7SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0147059Golgi
0.0664.57E-7SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.016129
0.0647.95E-7SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0561798TSC3-RPN4
0.0631.32E-6SGTC_32139129884 49.5 μMChembridge (Drug-like library)171907420.108434
0.0603.30E-6SGTC_1105epirubicin 18.1 μMNIH Clinical Collection653480.0952381anthracycline transcription coupled DNA repair
0.0594.87E-6SGTC_22907904846 200.0 μMChembridge (Fragment library)29621540.114286
0.0588.71E-6SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.123457RPP1 & pyrimidine depletion
0.0571.10E-5SGTC_13311472-0049 76.2 μMChemDiv (Drug-like library)240514090.0512821
0.0571.34E-5SGTC_13611608-0041 5.6 μMChemDiv (Drug-like library)X13610.0547945RPP1 & pyrimidine depletion
0.0561.62E-5SGTC_32189131285 49.5 μMChembridge (Drug-like library)171564170.0526316endomembrane recycling
0.0552.26E-5SGTC_21846075246 200.0 μMChembridge (Fragment library)22673200.0724638

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1704st02880570.8 μM0.4385962876101TimTec (Natural product derivative library)282.358622.5724
SGTC_2937904475311.33 μM0.4285716465530Chembridge (Drug-like library)393.543443.0921660S ribosome export
SGTC_10134112-3890241 μM0.363636705171ChemDiv (Drug-like library)331.386183.39725TSC3-RPN4
SGTC_2833900131545.46 μM0.3432842989378Chembridge (Drug-like library)358.411522.55425amide catabolism
SGTC_8034488-1585147 μM0.338462747204ChemDiv (Drug-like library)315.386783.924
SGTC_2808794646845.46 μM0.3285712970879Chembridge (Drug-like library)404.546163.75115
SGTC_9133487-1245167 μM0.3230774016153ChemDiv (Drug-like library)349.831844.53424
SGTC_1756st04155835.5 μM0.3214293955792TimTec (Natural product derivative library)564.649284.77928
SGTC_9751335-004628.5 μM0.31343316486740ChemDiv (Drug-like library)374.250142.67915RPP1 & pyrimidine depletion
SGTC_309k408-00511.48 μM0.3076923640463ChemDiv (Drug-like library)505.650043.32218