9128301

5-methyl-7-(4-methylpiperazin-1-yl)-3-phenyl-2-(trifluoromethyl)pyrazolo[1,5-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3222
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 4911358
SMILES CC1=NC2=C(C(=NN2C(=C1)N3CCN(CC3)C)C(F)(F)F)C4=CC=CC=C4
Standardized SMILES CN1CCN(CC1)c2cc(C)nc3c(c4ccccc4)c(nn23)C(F)(F)F
Molecular weight 375.3908
ALogP 3.93
H-bond donor count 0
H-bond acceptor count 7
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.89
% growth inhibition (Hom. pool) 9.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4911358
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:4.9|P-value:4.68E-7|Clearance:0.64||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:AVO1(YOL078W)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.03||SGD DESC:Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:CCT3(YJL014W)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:-5.59|P-value:1.12E-8|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-4.08|P-value:2.27E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.07||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC27(YBL084C)|FD-Score:3.12|P-value:8.95E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ERG11(YHR007C)|FD-Score:-5.15|P-value:1.29E-7|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:HTB1(YDR224C)|FD-Score:3.98|P-value:3.50E-5|Clearance:0.23||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IRA1(YBR140C)|FD-Score:-3.34|P-value:4.26E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:12.1|P-value:5.29E-34|Clearance:4.75||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM2(YBL026W)|FD-Score:-5.3|P-value:5.93E-8|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MNP1(YGL068W)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.09||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NMD3(YHR170W)|FD-Score:7.14|P-value:4.82E-13|Clearance:0.05||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP2(YNL061W)|FD-Score:-4.71|P-value:1.24E-6|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NUP159(YIL115C)|FD-Score:3.39|P-value:3.48E-4|Clearance:0.13||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP85(YJR042W)|FD-Score:7.35|P-value:9.64E-14|Clearance:0.22||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PRP3(YDR473C)|FD-Score:3.27|P-value:5.46E-4|Clearance:0.1||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RLP24(YLR009W)|FD-Score:-4.08|P-value:2.24E-5|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPL32(YBL092W)|FD-Score:-5.08|P-value:1.88E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN8(YOR261C)|FD-Score:-3.25|P-value:5.75E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPS20(YHL015W)|FD-Score:-3.11|P-value:9.31E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-5.06|P-value:2.04E-7|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP46(YGR095C)|FD-Score:-5|P-value:2.87E-7|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC26(YDR238C)|FD-Score:7.08|P-value:7.05E-13|Clearance:2.18||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEN1(YLR430W)|FD-Score:3.17|P-value:7.74E-4|Clearance:0.03||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SPN1(YPR133C)|FD-Score:-3.32|P-value:4.57E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAF12(YDR145W)|FD-Score:-4.93|P-value:4.20E-7|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF35(YDR429C)|FD-Score:-4.07|P-value:2.34E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UFD1(YGR048W)|FD-Score:3.74|P-value:9.03E-5|Clearance:0.2||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:UTP18(YJL069C)|FD-Score:-4.72|P-value:1.18E-6|Clearance:0||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:UTP4(YDR324C)|FD-Score:-4.55|P-value:2.66E-6|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:UTP6(YDR449C)|FD-Score:-5.84|P-value:2.54E-9|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGL102C(YGL102C_d)|FD-Score:3.09|P-value:9.96E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:YJL009W(YJL009W_d)|FD-Score:-3.47|P-value:2.64E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YKL111C(YKL111C_d)|FD-Score:4.27|P-value:9.86E-6|Clearance:0.29||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:YLR076C(YLR076C_d)|FD-Score:-4.57|P-value:2.38E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YOR146W(YOR146W_d)|FD-Score:-5.17|P-value:1.16E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPR136C(YPR136C_d)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ARC15(YIL062C)|FD-Score:4.9|P-value:4.68E-7|Clearance:0.64||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:AVO1(YOL078W)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.03||SGD DESC:Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:CCT3(YJL014W)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:-5.59|P-value:1.12E-8|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-4.08|P-value:2.27E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.07||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC27(YBL084C)|FD-Score:3.12|P-value:8.95E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ERG11(YHR007C)|FD-Score:-5.15|P-value:1.29E-7|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:HTB1(YDR224C)|FD-Score:3.98|P-value:3.50E-5|Clearance:0.23||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IRA1(YBR140C)|FD-Score:-3.34|P-value:4.26E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:12.1|P-value:5.29E-34|Clearance:4.75||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM2(YBL026W)|FD-Score:-5.3|P-value:5.93E-8|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MNP1(YGL068W)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.09||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NMD3(YHR170W)|FD-Score:7.14|P-value:4.82E-13|Clearance:0.05||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP2(YNL061W)|FD-Score:-4.71|P-value:1.24E-6|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NUP159(YIL115C)|FD-Score:3.39|P-value:3.48E-4|Clearance:0.13||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP85(YJR042W)|FD-Score:7.35|P-value:9.64E-14|Clearance:0.22||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PRP3(YDR473C)|FD-Score:3.27|P-value:5.46E-4|Clearance:0.1||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RLP24(YLR009W)|FD-Score:-4.08|P-value:2.24E-5|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPL32(YBL092W)|FD-Score:-5.08|P-value:1.88E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN8(YOR261C)|FD-Score:-3.25|P-value:5.75E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPS20(YHL015W)|FD-Score:-3.11|P-value:9.31E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-5.06|P-value:2.04E-7|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP46(YGR095C)|FD-Score:-5|P-value:2.87E-7|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC26(YDR238C)|FD-Score:7.08|P-value:7.05E-13|Clearance:2.18||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEN1(YLR430W)|FD-Score:3.17|P-value:7.74E-4|Clearance:0.03||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SPN1(YPR133C)|FD-Score:-3.32|P-value:4.57E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAF12(YDR145W)|FD-Score:-4.93|P-value:4.20E-7|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF35(YDR429C)|FD-Score:-4.07|P-value:2.34E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UFD1(YGR048W)|FD-Score:3.74|P-value:9.03E-5|Clearance:0.2||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:UTP18(YJL069C)|FD-Score:-4.72|P-value:1.18E-6|Clearance:0||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:UTP4(YDR324C)|FD-Score:-4.55|P-value:2.66E-6|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:UTP6(YDR449C)|FD-Score:-5.84|P-value:2.54E-9|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGL102C(YGL102C_d)|FD-Score:3.09|P-value:9.96E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:YJL009W(YJL009W_d)|FD-Score:-3.47|P-value:2.64E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YKL111C(YKL111C_d)|FD-Score:4.27|P-value:9.86E-6|Clearance:0.29||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:YLR076C(YLR076C_d)|FD-Score:-4.57|P-value:2.38E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YOR146W(YOR146W_d)|FD-Score:-5.17|P-value:1.16E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPR136C(YPR136C_d)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4911358
Download HOP data (tab-delimited text)  (excel)
Gene:ARP8(YOR141C)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:BEM4(YPL161C)|FD-Score:-4.81|P-value:7.44E-7||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BLI1(YKL061W_p)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNR1(YIL159W)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:CBR1(YIL043C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:COG8(YML071C)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX6(YHR051W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CWH43(YCR017C)|FD-Score:4.47|P-value:3.84E-6||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYT2(YKL087C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DHH1(YDL160C)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DIA1(YMR316W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DIG1(YPL049C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DRS2(YAL026C)|FD-Score:-3.68|P-value:1.14E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:EDE1(YBL047C)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:EGD1(YPL037C)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ELC1(YPL046C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:FKH2(YNL068C)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FSH3(YOR280C)|FD-Score:6.33|P-value:1.26E-10||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GET1(YGL020C)|FD-Score:-6.99|P-value:1.40E-12||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:HIR1(YBL008W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HIR2(YOR038C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p Gene:HUL5(YGL141W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:ICS3(YJL077C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:INO2(YDR123C)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:LEM3(YNL323W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM12(YHR121W)|FD-Score:4.29|P-value:8.91E-6||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MDM30(YLR368W)|FD-Score:-5.9|P-value:1.85E-9||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MOG1(YJR074W)|FD-Score:-5.18|P-value:1.10E-7||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL13(YKR006C)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPS9(YBR146W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB4(YOL112W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:NSG1(YHR133C)|FD-Score:6.09|P-value:5.49E-10||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OPT1(YJL212C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family Gene:OSW1(YOR255W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAN3(YKL025C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEX34(YCL056C)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:POL32(YJR043C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:RED1(YLR263W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RFM1(YOR279C)|FD-Score:7.77|P-value:3.93E-15||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGP1(YDR137W)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL26B(YGR034W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:6.61|P-value:1.94E-11||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:RTR2(YDR066C_p)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM4(YPL273W)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SEC66(YBR171W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFC1(YJR095W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SKI8(YGL213C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SNA2(YDR525W-A)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNQ2(YDR011W)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:STE20(YHL007C)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUM1(YDR310C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUV3(YPL029W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWA2(YDR320C)|FD-Score:-4.53|P-value:2.90E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWD3(YBR175W)|FD-Score:-3.96|P-value:3.76E-5||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:SWI4(YER111C)|FD-Score:4.33|P-value:7.44E-6||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TFB6(YOR352W)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TMA108(YIL137C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TVP18(YMR071C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBX4(YMR067C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VMA16(YHR026W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS3(YDR495C)|FD-Score:-4.34|P-value:6.98E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS65(YLR322W_d)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:VTS1(YOR359W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YAR030C(YAR030C_d)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YBL010C(YBL010C_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR064C(YCR064C_d)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YEL043W(YEL043W)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YGL007C-A(YGL007C-A_p)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGL015C(YGL015C_p)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGR069W(YGR069W_d)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR111W(YGR111W_p)|FD-Score:3.27|P-value:5.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR210C(YGR210C_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR080C(YHR080C)|FD-Score:5.38|P-value:3.64E-8||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YHR139C-A(YHR139C-A_d)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL182C(YJL182C_d)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YJR011C(YJR011C_p)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YJR018W(YJR018W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL162C(YKL162C_p)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YLR269C(YLR269C_d)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR013W-A(YMR013W-A_d)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR172C-A(YMR172C-A_d)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Putative protein of unknown function Gene:YNL193W(YNL193W_p)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOR072W(YOR072W_d)|FD-Score:6.06|P-value:6.62E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR385W(YOR385W_p)|FD-Score:7.14|P-value:4.83E-13||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPR126C(YPR126C_d)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ARP8(YOR141C)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:BEM4(YPL161C)|FD-Score:-4.81|P-value:7.44E-7||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BLI1(YKL061W_p)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNR1(YIL159W)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:CBR1(YIL043C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:COG8(YML071C)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX6(YHR051W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CWH43(YCR017C)|FD-Score:4.47|P-value:3.84E-6||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYT2(YKL087C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DHH1(YDL160C)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DIA1(YMR316W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DIG1(YPL049C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DRS2(YAL026C)|FD-Score:-3.68|P-value:1.14E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:EDE1(YBL047C)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:EGD1(YPL037C)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ELC1(YPL046C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:FKH2(YNL068C)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FSH3(YOR280C)|FD-Score:6.33|P-value:1.26E-10||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GET1(YGL020C)|FD-Score:-6.99|P-value:1.40E-12||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:HIR1(YBL008W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HIR2(YOR038C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p Gene:HUL5(YGL141W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:ICS3(YJL077C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:INO2(YDR123C)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:LEM3(YNL323W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM12(YHR121W)|FD-Score:4.29|P-value:8.91E-6||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MDM30(YLR368W)|FD-Score:-5.9|P-value:1.85E-9||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MOG1(YJR074W)|FD-Score:-5.18|P-value:1.10E-7||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL13(YKR006C)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPS9(YBR146W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB4(YOL112W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:NSG1(YHR133C)|FD-Score:6.09|P-value:5.49E-10||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OPT1(YJL212C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family Gene:OSW1(YOR255W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAN3(YKL025C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEX34(YCL056C)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:POL32(YJR043C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:RED1(YLR263W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RFM1(YOR279C)|FD-Score:7.77|P-value:3.93E-15||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGP1(YDR137W)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL26B(YGR034W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:6.61|P-value:1.94E-11||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:RTR2(YDR066C_p)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM4(YPL273W)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SEC66(YBR171W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFC1(YJR095W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SKI8(YGL213C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SNA2(YDR525W-A)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNQ2(YDR011W)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:STE20(YHL007C)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUM1(YDR310C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUV3(YPL029W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWA2(YDR320C)|FD-Score:-4.53|P-value:2.90E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWD3(YBR175W)|FD-Score:-3.96|P-value:3.76E-5||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:SWI4(YER111C)|FD-Score:4.33|P-value:7.44E-6||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TFB6(YOR352W)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TMA108(YIL137C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TVP18(YMR071C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBX4(YMR067C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VMA16(YHR026W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS3(YDR495C)|FD-Score:-4.34|P-value:6.98E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS65(YLR322W_d)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:VTS1(YOR359W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YAR030C(YAR030C_d)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YBL010C(YBL010C_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR064C(YCR064C_d)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YEL043W(YEL043W)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YGL007C-A(YGL007C-A_p)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGL015C(YGL015C_p)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGR069W(YGR069W_d)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR111W(YGR111W_p)|FD-Score:3.27|P-value:5.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR210C(YGR210C_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR080C(YHR080C)|FD-Score:5.38|P-value:3.64E-8||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YHR139C-A(YHR139C-A_d)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL182C(YJL182C_d)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YJR011C(YJR011C_p)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YJR018W(YJR018W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL162C(YKL162C_p)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YLR269C(YLR269C_d)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR013W-A(YMR013W-A_d)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR172C-A(YMR172C-A_d)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Putative protein of unknown function Gene:YNL193W(YNL193W_p)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOR072W(YOR072W_d)|FD-Score:6.06|P-value:6.62E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR385W(YOR385W_p)|FD-Score:7.14|P-value:4.83E-13||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPR126C(YPR126C_d)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W12.105.29E-344.75LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YJR042W7.359.64E-140.22NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YHR170W7.144.82E-130.05NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDR238C7.087.05E-132.18SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YIL062C4.904.68E-70.64ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YKL111C_d4.279.86E-60.29YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YDR224C3.983.50E-50.23HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YGR048W3.749.03E-50.20UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YHR107C3.551.95E-40.07CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YGL068W3.482.50E-40.09MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YIL115C3.393.48E-40.13NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YDR473C3.275.46E-40.10PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YLR430W3.177.74E-40.03SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YER112W3.138.62E-40.01LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YBL084C3.128.95E-40.00CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR279C7.773.93E-15RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YOR385W_p7.144.83E-13YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YMR030W6.611.94E-11RSF1Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
YOR280C6.331.26E-10FSH3Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2
YHR133C6.095.49E-10NSG1Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress
YOR072W_d6.066.62E-10YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YPL037C5.591.13E-8EGD1Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b
YHR080C5.383.64E-8YHR080CProtein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication
YKL061W_p5.191.06E-7BLI1_pPutative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
YOR352W5.032.46E-7TFB6Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YEL043W4.671.49E-6YEL043WPredicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold
YBL047C4.562.61E-6EDE1Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death
YCR017C4.473.84E-6CWH43Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
YJL182C_d4.473.94E-6YJL182C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W
YKR006C4.444.52E-6MRPL13Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation

GO enrichment analysis for SGTC_3222
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2831.79E-109SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.13580260S ribosome export
0.2391.24E-77SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.069767460S ribosome export
0.2353.76E-75SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.10526360S ribosome export
0.2344.25E-74SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.083333360S ribosome export
0.2162.34E-63SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.18918960S ribosome export
0.2132.01E-61SGTC_14043909-7820 48.3 μMChemDiv (Drug-like library)237413730.14102660S ribosome export
0.2107.37E-60SGTC_1664st014217 9.0 μMTimTec (Natural product derivative library)39827820.077777860S ribosome export
0.2041.91E-56SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.093333360S ribosome export
0.2011.13E-54SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.14473760S ribosome export
0.1997.73E-54SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.11267660S ribosome export
0.1991.03E-53SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.071428660S ribosome export
0.1991.10E-53SGTC_32499135183 49.5 μMChembridge (Drug-like library)49027790.35616460S ribosome export
0.1962.23E-52SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.069767460S ribosome export
0.1942.21E-51SGTC_1786berberine 21.6 μMMiscellaneous124560.069767460S ribosome export
0.1864.53E-47SGTC_32809140961 49.5 μMChembridge (Drug-like library)49012260.56896560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3329914123327.56 μM0.754909101Chembridge (Drug-like library)405.416793.39418
SGTC_3328914062429.04 μM0.6111114904392Chembridge (Drug-like library)335.4463.33104
SGTC_3280914096149.47 μM0.5689654901226Chembridge (Drug-like library)355.864483.5090460S ribosome export
SGTC_3333914182012.09 μM0.5689651939433Chembridge (Drug-like library)349.472583.97404
SGTC_3343914837918.98 μM0.551955036Chembridge (Drug-like library)349.472583.96804
SGTC_3257913920632.98 μM0.4745764910041Chembridge (Drug-like library)321.419423.04804
SGTC_334591484103.56 μM0.4461544903596Chembridge (Drug-like library)413.514783.9715
SGTC_3339914588314.94 μM0.4264712030670Chembridge (Drug-like library)379.498563.43515
SGTC_3235913260249.47 μM0.4218754912240Chembridge (Drug-like library)352.430163.31605
SGTC_3326914006416.38 μM0.420291868434Chembridge (Drug-like library)379.498563.42815