9131256

2,4-difluoro-N-[3-(oxolan-2-ylmethoxy)phenyl]benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3226
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17178073
SMILES C1CC(OC1)COC2=CC=CC(=C2)NC(=O)C3=C(C=C(C=C3)F)F
Standardized SMILES Fc1ccc(C(=O)Nc2cccc(OCC3CCCO3)c2)c(F)c1
Molecular weight 333.3293
ALogP 3.37
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.78
% growth inhibition (Hom. pool) 5.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17178073
Download HIP data (tab-delimited text)  (excel)
Gene:ALG7(YBR243C)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.28||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:ARP7(YPR034W)|FD-Score:3.16|P-value:7.93E-4|Clearance:0.28||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DRE2(YKR071C)|FD-Score:-4.23|P-value:1.15E-5|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FCF1(YDR339C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.28||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:ALG7(YBR243C)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.28||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:ARP7(YPR034W)|FD-Score:3.16|P-value:7.93E-4|Clearance:0.28||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DRE2(YKR071C)|FD-Score:-4.23|P-value:1.15E-5|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FCF1(YDR339C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.28||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17178073
Download HOP data (tab-delimited text)  (excel)
Gene:FAA1(YOR317W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FTR1(YER145C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:IXR1(YKL032C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:MIC23(YBL107C_p)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MPC54(YOR177C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL20(YKR085C)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH5(YDL154W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NMA1(YLR328W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:NMA111(YNL123W)|FD-Score:-3.76|P-value:8.41E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:PUT3(YKL015W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:SAN1(YDR143C)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SOL2(YCR073W-A)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SUN4(YNL066W)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:TRS33(YOR115C)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:VPS52(YDR484W)|FD-Score:-4.86|P-value:5.82E-7||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:FAA1(YOR317W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FTR1(YER145C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:IXR1(YKL032C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:MIC23(YBL107C_p)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MPC54(YOR177C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL20(YKR085C)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH5(YDL154W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NMA1(YLR328W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:NMA111(YNL123W)|FD-Score:-3.76|P-value:8.41E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:PUT3(YKL015W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:SAN1(YDR143C)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SOL2(YCR073W-A)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SUN4(YNL066W)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:TRS33(YOR115C)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:VPS52(YDR484W)|FD-Score:-4.86|P-value:5.82E-7||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR339C3.275.33E-40.28FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YBR243C3.177.69E-40.28ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YPR034W3.167.93E-40.28ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YLR026C2.960.001520.28SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YLR259C2.820.002380.28HSP60Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
YMR268C2.740.003040.28PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YEL058W2.690.003580.28PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YPL063W2.650.004020.28TIM50Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
YOL120C2.370.008960.09RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YIR012W2.270.011500.01SQT1Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
YGL061C2.270.011700.00DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YLR339C_d2.260.011800.04YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
YCL043C2.220.013100.00PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YPR113W2.220.013100.01PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YGL155W2.210.013600.01CDC43Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR177C4.201.32E-5MPC54Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate
YKL015W3.492.44E-4PUT3Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
YOR317W3.363.92E-4FAA1Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication
YKL032C3.354.11E-4IXR1Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b
YDL154W3.344.17E-4MSH5Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
YER145C3.109.55E-4FTR1High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
YEL071W3.070.00108DLD3D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
YPR158W3.050.00116CUR1Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication
YGL026C2.810.00246TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YGL226C-A2.810.00247OST5Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YGR213C2.760.00289RTA1Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
YGR138C2.670.00374TPO2Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
YFL004W2.670.00382VTC2Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion
YEL063C2.660.00387CAN1Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
YPR037C2.650.00397ERV2Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER

GO enrichment analysis for SGTC_3226
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2214.41E-66SGTC_32309131665 49.5 μMChembridge (Drug-like library)159437200.105263
0.1194.97E-20SGTC_30869117350 49.5 μMChembridge (Drug-like library)26792650.217949
0.0991.86E-14SGTC_30969117468 49.5 μMChembridge (Drug-like library)413163110.113924
0.0931.00E-12SGTC_31079121808 49.5 μMChembridge (Drug-like library)6626810.103448
0.0855.84E-11SGTC_28869058944 52.0 μMChembridge (Drug-like library)49499290.16
0.0791.53E-9SGTC_411enrofloxacin 100.0 μMMiscellaneous711880.149425DNA damage response
0.0764.48E-9SGTC_800159-0065 512.2 μMChemDiv (Drug-like library)15480790.108696
0.0731.64E-8SGTC_255tunicamycin 200.5 nMICCB bioactive library238471770.0854701unfolded protein response
0.0722.64E-8SGTC_1789tunicamycin 25.0 nMICCB bioactive library238471770.0854701unfolded protein response
0.0714.81E-8SGTC_11383761-0006 1.0 μMChemDiv (Drug-like library)120610870.0487805
0.0714.68E-8SGTC_32629137218 49.5 μMChembridge (Drug-like library)176506640.131579
0.0715.69E-8SGTC_840443-0269 128.8 μMChemDiv (Drug-like library)61500580.0853659
0.0706.64E-8SGTC_440929-0055 61.2 μMChemDiv (Drug-like library)13795900.0657895
0.0706.16E-8SGTC_31959113082 49.5 μMChembridge (Drug-like library)170180380.162162
0.0672.59E-7SGTC_23296138490 200.0 μMChembridge (Fragment library)7200190.0769231

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3103912082249.47 μM0.63934417141819Chembridge (Drug-like library)327.374342.9414
SGTC_3092911724849.47 μM0.61666717141857Chembridge (Drug-like library)346.808063.52923cell wall signaling
SGTC_3072911641949.47 μM0.40579717170162Chembridge (Drug-like library)331.793423.62113ERG2
SGTC_2942905277762.25 μM0.3934436469503Chembridge (Drug-like library)284.1131234.14912
SGTC_1918oxolan-2-ylmethyl 3-bromobenzoate70.1 μM0.384615551469TimTec (Natural product derivative library)285.133822.79203
SGTC_2889905965651.95 μM0.3833333949838Chembridge (Drug-like library)287.1848563.64216
SGTC_3173910569349.47 μM0.37878817096658Chembridge (Drug-like library)289.756744.13512
SGTC_3098911903949.47 μM0.37313417161528Chembridge (Drug-like library)287.3287234.05313Golgi
SGTC_3184910875849.47 μM0.370968902885Chembridge (Drug-like library)241.28512.94712
SGTC_2883905914545.46 μM0.35820916476510Chembridge (Drug-like library)269.2703833.99613