9131799

5-amino-N-(2-methylphenyl)-1-(3-methylphenyl)triazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3228
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17015818
SMILES CC1=CC(=CC=C1)N2C(=C(N=N2)C(=O)NC3=CC=CC=C3C)N
Standardized SMILES Cc1cccc(c1)n2nnc(C(=O)Nc3ccccc3C)c2N
Molecular weight 307.3498
ALogP 3.15
H-bond donor count 2
H-bond acceptor count 4
Response signature amide catabolism

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.59
% growth inhibition (Hom. pool) 11.75


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17015818
Download HIP data (tab-delimited text)  (excel)
Gene:CAB3(YKL088W)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.09||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:DML1(YMR211W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.28||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:KRR1(YCL059C)|FD-Score:3.81|P-value:7.08E-5|Clearance:0.21||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:PDC2(YDR081C)|FD-Score:4.22|P-value:1.22E-5|Clearance:0.33||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:RLP24(YLR009W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.3||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RSC3(YDR303C)|FD-Score:-3.5|P-value:2.35E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:CAB3(YKL088W)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.09||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:DML1(YMR211W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.28||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:KRR1(YCL059C)|FD-Score:3.81|P-value:7.08E-5|Clearance:0.21||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:PDC2(YDR081C)|FD-Score:4.22|P-value:1.22E-5|Clearance:0.33||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:RLP24(YLR009W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.3||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RSC3(YDR303C)|FD-Score:-3.5|P-value:2.35E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17015818
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:BNI4(YNL233W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BTS1(YPL069C)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:DAL81(YIR023W)|FD-Score:12.5|P-value:5.84E-36||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:GCN5(YGR252W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCV2(YMR189W)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:HO(YDL227C)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p Gene:HSP31(YDR533C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:ICL2(YPR006C)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:LIP2(YLR239C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:NGG1(YDR176W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PIC2(YER053C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature Gene:PIN3(YPR154W)|FD-Score:-3.78|P-value:8.00E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PPH22(YDL188C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PRD1(YCL057W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RPL42B(YHR141C)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SLY41(YOR307C)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SPC2(YML055W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SSD1(YDR293C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TDA5(YLR426W_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TDP1(YBR223C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TPN1(YGL186C)|FD-Score:-3.73|P-value:9.48E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:URM1(YIL008W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS25(YJR102C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR184W(YBR184W_p)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBR238C(YBR238C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCR022C(YCR022C_d)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YDL172C(YDL172C_d)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGR054W(YGR054W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YLR345W(YLR345W_p)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR406C-A(YLR406C-A_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Putative protein of unknown function Gene:ADO1(YJR105W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:BNI4(YNL233W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BTS1(YPL069C)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:DAL81(YIR023W)|FD-Score:12.5|P-value:5.84E-36||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:GCN5(YGR252W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCV2(YMR189W)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:HO(YDL227C)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p Gene:HSP31(YDR533C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:ICL2(YPR006C)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:LIP2(YLR239C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:NGG1(YDR176W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PIC2(YER053C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature Gene:PIN3(YPR154W)|FD-Score:-3.78|P-value:8.00E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PPH22(YDL188C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PRD1(YCL057W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RPL42B(YHR141C)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SLY41(YOR307C)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SPC2(YML055W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SSD1(YDR293C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TDA5(YLR426W_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TDP1(YBR223C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TPN1(YGL186C)|FD-Score:-3.73|P-value:9.48E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:URM1(YIL008W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS25(YJR102C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR184W(YBR184W_p)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBR238C(YBR238C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCR022C(YCR022C_d)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YDL172C(YDL172C_d)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGR054W(YGR054W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YLR345W(YLR345W_p)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR406C-A(YLR406C-A_p)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR081C4.221.22E-50.33PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YKL088W3.894.98E-50.09CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YCL059C3.817.08E-50.21KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
YLR009W3.601.61E-40.30RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YMR211W3.304.83E-40.28DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YGR115C_d3.020.001270.14YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YNL149C2.880.001990.04PGA2Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
YOR294W2.840.002270.02RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YDR460W2.820.002438.33E-4TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YHR062C2.810.002440.01RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDL152W_d2.810.002500.11YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YKL172W2.690.003520.01EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YOR157C2.690.003610.02PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YOL022C2.670.003810.06TSR4Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
YGR119C2.610.004570.03NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W12.505.84E-36DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YPL069C4.444.51E-6BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YDL172C_d4.191.37E-5YDL172C_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
YCL057W4.161.58E-5PRD1Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress
YBR184W_p3.865.63E-5YBR184W_pPutative protein of unknown function; YBR184W is not an essential gene
YDL227C3.797.63E-5HOSite-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p
YER076C_p3.778.01E-5YER076C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
YML055W3.671.22E-4SPC2Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25
YGR252W3.661.26E-4GCN5Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
YGR054W3.631.40E-4YGR054WEukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A
YDR176W3.611.52E-4NGG1Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
YJR105W3.591.63E-4ADO1Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
YNL233W3.571.75E-4BNI4Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p
YDL188C3.542.02E-4PPH22Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
YLR426W_p3.512.22E-4TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele

GO enrichment analysis for SGTC_3228
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3214.13E-141SGTC_486niguldipine 82.0 μMMiscellaneous12360.122449amide catabolism
0.3162.34E-137SGTC_24605552655 174.2 μMMiscellaneous54019890.15942amide catabolism
0.2621.36E-93SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.169014amide catabolism
0.2428.39E-80SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.105882amide catabolism
0.2332.80E-73SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.138889amide catabolism
0.2312.52E-72SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.755102amide catabolism
0.2142.31E-62SGTC_5694130-2812 160.0 μMChemDiv (Drug-like library)7435520.121212amide catabolism
0.2042.24E-56SGTC_10563448-1962 115.0 μMChemDiv (Drug-like library)7155070.130435amide catabolism
0.2015.56E-55SGTC_3252810-4341 181.0 μMChemDiv (Drug-like library)7749700.138889amide catabolism
0.1971.14E-52SGTC_31679103034 49.5 μMChembridge (Drug-like library)51278590.205882RPP1 & pyrimidine depletion
0.1963.73E-52SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.371429
0.1842.75E-46SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.144928amide catabolism
0.1689.36E-39SGTC_253dyclonine 31.2 μMMiscellaneous31800.0921053amide catabolism
0.1624.21E-36SGTC_1759st043059 70.0 μMTimTec (Natural product derivative library)6694260.265625amide catabolism
0.1541.65E-32SGTC_563tetrahydrosecaminediol 614.0 μMChemDiv (Drug-like library)3342740.157895amide catabolism

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3274913829849.47 μM0.80434817015756Chembridge (Drug-like library)293.32322.66624
SGTC_3225913081949.47 μM0.7551024547121Chembridge (Drug-like library)293.32322.66624amide catabolism
SGTC_3271913779849.47 μM0.6071436498326Chembridge (Drug-like library)299.370842.94824
SGTC_3245913482149.47 μM0.59259317014901Chembridge (Drug-like library)307.349783.12224
SGTC_3270913756349.47 μM0.53703717015724Chembridge (Drug-like library)293.32322.66624RPP1 & pyrimidine depletion
SGTC_3220913126949.47 μM0.517722206Chembridge (Drug-like library)377.439623.68324
SGTC_3355915105844.05 μM0.517015728Chembridge (Drug-like library)311.3136632.87125RNA processing & uracil transport
SGTC_3263913701849.47 μM0.48387143844957Chembridge (Drug-like library)336.38773.20624
SGTC_3192911036249.47 μM0.4761916649480Chembridge (Drug-like library)358.393223.83914mitochondrial processes
SGTC_3212912830749.47 μM0.45901627243542Chembridge (Drug-like library)350.213742.75424