9132874

methyl 3-(phenylcarbamoyl)benzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3232
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 3784940
SMILES COC(=O)C1=CC=CC(=C1)C(=O)NC2=CC=CC=C2
Standardized SMILES COC(=O)c1cccc(c1)C(=O)Nc2ccccc2
Molecular weight 255.2686
ALogP 2.47
H-bond donor count 1
H-bond acceptor count 3
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.52
% growth inhibition (Hom. pool) 3.66


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3784940
Download HIP data (tab-delimited text)  (excel)
Gene:CDC39(YCR093W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.16||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CEF1(YMR213W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.01||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:GCD11(YER025W)|FD-Score:5.15|P-value:1.27E-7|Clearance:0.3||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:11.2|P-value:3.38E-29|Clearance:5.58||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KRR1(YCL059C)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.12||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:LSM2(YBL026W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MED8(YBR193C)|FD-Score:4.71|P-value:1.23E-6|Clearance:0.5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:PBN1(YCL052C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.01||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PKC1(YBL105C)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RHO1(YPR165W)|FD-Score:4.07|P-value:2.39E-5|Clearance:0.45||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPA190(YOR341W)|FD-Score:3.11|P-value:9.42E-4|Clearance:0.02||SGD DESC:RNA polymerase I largest subunit A190 Gene:RRP15(YPR143W)|FD-Score:3.41|P-value:3.19E-4|Clearance:0.03||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:SEC31(YDL195W)|FD-Score:5.57|P-value:1.24E-8|Clearance:0.42||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SUI2(YJR007W)|FD-Score:4.21|P-value:1.29E-5|Clearance:0.09||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:3.39|P-value:3.50E-4|Clearance:0.09||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF35(YDR429C)|FD-Score:4.86|P-value:5.97E-7|Clearance:0.15||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TUB1(YML085C)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.02||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP6(YDR449C)|FD-Score:3.29|P-value:4.92E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPL238C(YPL238C_d)|FD-Score:4.12|P-value:1.92E-5|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:CDC39(YCR093W)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.16||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CEF1(YMR213W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.01||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:GCD11(YER025W)|FD-Score:5.15|P-value:1.27E-7|Clearance:0.3||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:11.2|P-value:3.38E-29|Clearance:5.58||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KRR1(YCL059C)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.12||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:LSM2(YBL026W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MED8(YBR193C)|FD-Score:4.71|P-value:1.23E-6|Clearance:0.5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:PBN1(YCL052C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.01||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PKC1(YBL105C)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RHO1(YPR165W)|FD-Score:4.07|P-value:2.39E-5|Clearance:0.45||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPA190(YOR341W)|FD-Score:3.11|P-value:9.42E-4|Clearance:0.02||SGD DESC:RNA polymerase I largest subunit A190 Gene:RRP15(YPR143W)|FD-Score:3.41|P-value:3.19E-4|Clearance:0.03||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:SEC31(YDL195W)|FD-Score:5.57|P-value:1.24E-8|Clearance:0.42||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SUI2(YJR007W)|FD-Score:4.21|P-value:1.29E-5|Clearance:0.09||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:3.39|P-value:3.50E-4|Clearance:0.09||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF35(YDR429C)|FD-Score:4.86|P-value:5.97E-7|Clearance:0.15||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TUB1(YML085C)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.02||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP6(YDR449C)|FD-Score:3.29|P-value:4.92E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPL238C(YPL238C_d)|FD-Score:4.12|P-value:1.92E-5|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3784940
Download HOP data (tab-delimited text)  (excel)
Gene:AIM14(YGL160W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ALG5(YPL227C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APM3(YBR288C)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ARF2(YDL137W)|FD-Score:-4.43|P-value:4.62E-6||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ASN2(YGR124W)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG15(YCR068W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:CAN1(YEL063C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAP2(YIL034C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCR4(YAL021C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCW12(YLR110C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CGI121(YML036W)|FD-Score:-4.01|P-value:3.05E-5||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COA4(YLR218C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COG5(YNL051W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DID4(YKL002W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:EMC3(YKL207W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FKH2(YNL068C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:GLK1(YCL040W)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:HCR1(YLR192C)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HCS1(YKL017C)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HOP1(YIL072W)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:ISC1(YER019W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:MFM1(YPL060W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MRP2(YPR166C)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS12(YNR036C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MXR2(YCL033C)|FD-Score:3.8|P-value:7.19E-5||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NMA111(YNL123W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NTH2(YBR001C)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PHM8(YER037W)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:RGI1(YER067W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RHO2(YNL090W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:ROX3(YBL093C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL36B(YPL249C-A)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0A(YGR214W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:SAM37(YMR060C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SCO2(YBR024W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SIN4(YNL236W)|FD-Score:8.17|P-value:1.50E-16||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SLH1(YGR271W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLX9(YGR081C)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNA2(YDR525W-A)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOD2(YHR008C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:STB2(YMR053C)|FD-Score:3.94|P-value:3.99E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SYO1(YDL063C)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRM1(YDR120C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRP2(YER090W)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-4.31|P-value:8.10E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VAM10(YOR068C)|FD-Score:6.72|P-value:9.33E-12||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VAN1(YML115C)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VMA13(YPR036W)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS13(YLL040C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS63(YLR261C_d)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR277C(YBR277C_d)|FD-Score:5.21|P-value:9.52E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCH1(YGR203W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YCL046W(YCL046W_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDL118W(YDL118W_p)|FD-Score:-3.94|P-value:4.05E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YER034W(YER034W_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER119C-A(YER119C-A_d)|FD-Score:5.03|P-value:2.41E-7||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFL015C(YFL015C_d)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YFL019C(YFL019C_d)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGL159W(YGL159W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR051C(YGR051C_d)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YGR269W(YGR269W_d)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YKL147C(YKL147C_d)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL190W(YNL190W_p)|FD-Score:5.35|P-value:4.34E-8||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:AIM14(YGL160W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ALG5(YPL227C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APM3(YBR288C)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ARF2(YDL137W)|FD-Score:-4.43|P-value:4.62E-6||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ASN2(YGR124W)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG15(YCR068W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:CAN1(YEL063C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAP2(YIL034C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCR4(YAL021C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCW12(YLR110C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CGI121(YML036W)|FD-Score:-4.01|P-value:3.05E-5||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COA4(YLR218C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COG5(YNL051W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DID4(YKL002W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:EMC3(YKL207W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FKH2(YNL068C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:GLK1(YCL040W)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:HCR1(YLR192C)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HCS1(YKL017C)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HOP1(YIL072W)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:ISC1(YER019W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:MFM1(YPL060W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MRP2(YPR166C)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS12(YNR036C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MXR2(YCL033C)|FD-Score:3.8|P-value:7.19E-5||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NMA111(YNL123W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NTH2(YBR001C)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PHM8(YER037W)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:RGI1(YER067W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RHO2(YNL090W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:ROX3(YBL093C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL36B(YPL249C-A)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0A(YGR214W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:SAM37(YMR060C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SCO2(YBR024W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SIN4(YNL236W)|FD-Score:8.17|P-value:1.50E-16||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SLH1(YGR271W)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLX9(YGR081C)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNA2(YDR525W-A)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOD2(YHR008C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:STB2(YMR053C)|FD-Score:3.94|P-value:3.99E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SYO1(YDL063C)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRM1(YDR120C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRP2(YER090W)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-4.31|P-value:8.10E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VAM10(YOR068C)|FD-Score:6.72|P-value:9.33E-12||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VAN1(YML115C)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VMA13(YPR036W)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS13(YLL040C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS63(YLR261C_d)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR277C(YBR277C_d)|FD-Score:5.21|P-value:9.52E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCH1(YGR203W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YCL046W(YCL046W_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDL118W(YDL118W_p)|FD-Score:-3.94|P-value:4.05E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YER034W(YER034W_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER119C-A(YER119C-A_d)|FD-Score:5.03|P-value:2.41E-7||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFL015C(YFL015C_d)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YFL019C(YFL019C_d)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGL159W(YGL159W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR051C(YGR051C_d)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YGR269W(YGR269W_d)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YKL147C(YKL147C_d)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YMR172C-A(YMR172C-A_d)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL190W(YNL190W_p)|FD-Score:5.35|P-value:4.34E-8||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER133W11.203.38E-295.58GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDL195W5.571.24E-80.42SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YER025W5.151.27E-70.30GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YDR429C4.865.97E-70.14TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YBR193C4.711.23E-60.50MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YJR007W4.211.29E-50.09SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YPL238C_d4.121.92E-50.05YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YPR165W4.072.39E-50.45RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YCL052C3.621.49E-40.01PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YMR213W3.601.58E-40.01CEF1Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
YBL026W3.591.64E-40.04LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YML085C3.551.93E-40.02TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YCL059C3.532.08E-40.12KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
YPR143W3.413.19E-40.03RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YPL237W3.393.50E-40.09SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL236W8.171.50E-16SIN4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
YOR068C6.729.33E-12VAM10Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
YNL190W_p5.354.34E-8YNL190W_pHydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site
YBR277C_d5.219.52E-8YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
YKL017C5.151.30E-7HCS1Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
YER119C-A_d5.032.41E-7YER119C-A_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
YFL019C_d4.701.31E-6YFL019C_dDubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene
YIL072W4.641.76E-6HOP1Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments
YGR124W4.424.90E-6ASN2Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
YLR192C4.356.95E-6HCR1Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA
YFL015C_d4.121.87E-5YFL015C_dDubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene
YPL249C-A3.973.58E-5RPL36BRibosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
YMR053C3.943.99E-5STB2Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p
YKL002W3.914.64E-5DID4Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
YCL040W3.895.08E-5GLK1Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3232
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2251.67E-68SGTC_1766st045414 44.8 μMTimTec (Natural product derivative library)2539600.0923077RPP1 & pyrimidine depletion
0.1771.07E-42SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.230769
0.1661.36E-37SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.222222RPP1 & pyrimidine depletion
0.1653.01E-37SGTC_20575236571 38.3 μMChembridge (Fragment library)54684039NARPP1 & pyrimidine depletion
0.1611.04E-35SGTC_21565649887 115.0 μMChembridge (Fragment library)54680440NARPP1 & pyrimidine depletion
0.1604.85E-35SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.166667RPP1 & pyrimidine depletion
0.1526.66E-32SGTC_1959st077091 55.8 μMTimTec (Natural product derivative library)8213560.172414RPP1 & pyrimidine depletion
0.1411.04E-27SGTC_6001495-0418 41.6 μMChemDiv (Drug-like library)53777680.230769RPP1 & pyrimidine depletion
0.1343.90E-25SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.0985916
0.1292.93E-23SGTC_2489cantharidin 73.4 μMICCB bioactive library25450.0612245cell wall signaling
0.1193.28E-20SGTC_13853091-4834 14.1 μMChemDiv (Drug-like library)40728140.155172RPP1 & pyrimidine depletion
0.1195.09E-20SGTC_28107959871 45.5 μMChembridge (Drug-like library)29745350.234375copper-dependent oxidative stress
0.1196.46E-20SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.0612245cell wall signaling
0.1181.08E-19SGTC_6293448-6466 76.7 μMChemDiv (Drug-like library)5737440.259259
0.1164.39E-19SGTC_1625st003711 30.1 μMTimTec (Natural product derivative library)242070340.135802calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5544483-2211187 μM0.692308747107ChemDiv (Drug-like library)285.29462.45314
SGTC_23157733312200 μM0.575726316Chembridge (Fragment library)210.25291.93523
SGTC_3203911354349.47 μM0.56097617018012Chembridge (Drug-like library)241.28513.08412
SGTC_3163909991449.47 μM0.54347816268368Chembridge (Drug-like library)260.245381.49815
SGTC_3182910884949.47 μM0.53333317133045Chembridge (Drug-like library)267.322383.66112RPP1 & pyrimidine depletion
SGTC_3184910875849.47 μM0.511628902885Chembridge (Drug-like library)241.28512.94712
SGTC_1759st04305970 μM0.511111669426TimTec (Natural product derivative library)285.29462.45314amide catabolism
SGTC_2926901072665.15 μM0.5111112994293Chembridge (Drug-like library)253.29583.21512
SGTC_7161683-5115658 μM0.5723597ChemDiv (Drug-like library)303.740263.48313
SGTC_12550566-0045221 μM0.52788469ChemDiv (Drug-like library)304.728323.04223excess fatty acid