9133421

3-[4-(3-chlorophenyl)piperazine-1-carbonyl]-6,7,8,9-tetrahydropyrimido[2,1-b][1,3]benzothiazol-4-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3241
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17018221
SMILES C1CCC2=C(C1)N3C(=O)C(=CN=C3S2)C(=O)N4CCN(CC4)C5=CC(=CC=C5)Cl
Standardized SMILES Clc1cccc(c1)N2CCN(CC2)C(=O)C3=CN=C4SC5=C(CCCC5)N4C3=O
Molecular weight 428.935
ALogP 3.25
H-bond donor count 0
H-bond acceptor count 5
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.21
% growth inhibition (Hom. pool) 8.89


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17018221
Download HIP data (tab-delimited text)  (excel)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17018221
Download HOP data (tab-delimited text)  (excel)
Gene:GAL11(YOL051W)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:MSA1(YOR066W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:OCA6(YDR067C_p)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PDR1(YGL013C)|FD-Score:6.51|P-value:3.70E-11||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:POL4(YCR014C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:SNT1(YCR033W)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SPT3(YDR392W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRO77(YBL106C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:TCB1(YOR086C)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:VPS68(YOL129W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YCR022C(YCR022C_d)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YGR054W(YGR054W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YGR111W(YGR111W_p)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:GAL11(YOL051W)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:MSA1(YOR066W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:OCA6(YDR067C_p)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PDR1(YGL013C)|FD-Score:6.51|P-value:3.70E-11||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:POL4(YCR014C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:SNT1(YCR033W)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SPT3(YDR392W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRO77(YBL106C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:TCB1(YOR086C)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:VPS68(YOL129W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YCR022C(YCR022C_d)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YGR054W(YGR054W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YGR111W(YGR111W_p)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR253C4.171.55E-51.16PUP2Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
YKL035W3.010.001300.01UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YLR409C3.000.001360.24UTP21Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma
YOR340C2.760.002910.01RPA43RNA polymerase I subunit A43
YOR257W2.740.003030.31CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YDR341C2.430.007500.04YDR341CArginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOR160W2.390.008340.02MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YNL062C2.370.008780.08GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YCL041C_d2.300.010800.02YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YPL210C2.280.011300.06SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YBR038W2.220.013300.04CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YOR353C2.180.014800.02SOG2Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
YPR082C2.160.015600.02DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YOR176W2.130.016500.03HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YDL139C2.110.017600.06SCM3Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C6.513.70E-11PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YOL129W3.571.82E-4VPS68Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria
YGR111W_p3.393.45E-4YGR111W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YGL025C3.295.04E-4PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YBL106C3.206.81E-4SRO77Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
YCR014C3.197.05E-4POL4DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
YCR022C_d3.167.87E-4YCR022C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene
YDR392W3.138.70E-4SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YCR033W3.129.05E-4SNT1Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance
YOL051W3.129.18E-4GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YDR067C_p3.119.38E-4OCA6_pCytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
YCR100C_p3.070.00108YCR100C_pPutative protein of unknown function
YNL237W3.060.00111YTP1Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
YOR134W3.020.00126BAG7Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p
YOR200W_d2.990.00139YOR200W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c

GO enrichment analysis for SGTC_3241
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3781.61E-199SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0666667PDR1
0.3518.74E-171SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0930233PDR1
0.2892.17E-114SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.0693069ERG2
0.2892.31E-114SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.0561798sphingolipid biosynthesis & PDR1
0.2611.44E-92SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.121951PDR1
0.2605.91E-92SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.111111PDR1
0.2576.45E-90SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.0666667PDR1
0.2576.20E-90SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.119048PDR1
0.2501.31E-84SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.15PDR1
0.2441.66E-80SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.0947368PDR1
0.2355.47E-75SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0686275PDR1
0.2343.50E-74SGTC_32569138902 49.5 μMChembridge (Drug-like library)172287730.0823529PDR1
0.2131.28E-61SGTC_12870971-0001 34.6 μMChemDiv (Drug-like library)46658690.0722892sphingolipid biosynthesis & PDR1
0.2104.14E-60SGTC_196k015-0023 18.1 μMChemDiv (Drug-like library)52119980.0930233PDR1
0.2057.31E-57SGTC_489paxilline 115.0 μMICCB bioactive library1050080.0642202PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3242913394049.47 μM0.46376817248444Chembridge (Drug-like library)305.395261.94414
SGTC_12830922-012516.4 μM0.3333333360884ChemDiv (Drug-like library)425.951024.84904
SGTC_3223912997049.47 μM0.31081117248386Chembridge (Drug-like library)327.400782.62614
SGTC_3324913875960.75 μM0.28571445595710Chembridge (Drug-like library)375.333123.75624
SGTC_3045909295049.47 μM0.2844120432Chembridge (Drug-like library)365.296784.31712
SGTC_3049909222349.47 μM0.288623917Chembridge (Drug-like library)328.835843.93502
SGTC_11564092-03674.88 μM0.2763161357585ChemDiv (Drug-like library)382.926285.00702
SGTC_3137909735249.47 μM0.27272744120435Chembridge (Drug-like library)371.32453.9313
SGTC_2147554593289.72 μM0.257576797404Chembridge (Fragment library)232.32142.33802ergosterol biosynthesis
SGTC_16794130-12766.7 μM0.256411005053Miscellaneous363.7707033.63705Golgi