9134147

1-(4,8-dimethylquinolin-2-yl)sulfanylpropan-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3243
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 24282180
SMILES CC1=CC=CC2=C1N=C(C=C2C)SCC(=O)C
Standardized SMILES CC(=O)CSc1cc(C)c2cccc(C)c2n1
Molecular weight 245.34
ALogP 3.65
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 49.1
% growth inhibition (Hom. pool) 11.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24282180
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:-3.23|P-value:6.14E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC24(YAL041W)|FD-Score:-5.3|P-value:5.76E-8|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:GCD10(YNL062C)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.06||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:4.91|P-value:4.64E-7|Clearance:0.57||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HYP2(YEL034W)|FD-Score:-3.54|P-value:2.00E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:INO80(YGL150C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.12||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:IRR1(YIL026C)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MNP1(YGL068W)|FD-Score:4.33|P-value:7.37E-6|Clearance:0.18||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NOP56(YLR197W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.05||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUS1(YDL193W)|FD-Score:-5.54|P-value:1.49E-8|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRI1(YIR008C)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.14||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RER2(YBR002C)|FD-Score:-4.75|P-value:1.02E-6|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB7(YDR404C)|FD-Score:-4.51|P-value:3.26E-6|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC10(YHR143W-A)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPO26(YPR187W)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RSC4(YKR008W)|FD-Score:-3.28|P-value:5.13E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC6(YCR052W)|FD-Score:-4.25|P-value:1.07E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:-3.13|P-value:8.72E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC11(YIR022W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.04||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SEC6(YIL068C)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.53||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SRP72(YPL210C)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.15||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:SUI2(YJR007W)|FD-Score:-3.52|P-value:2.14E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUP35(YDR172W)|FD-Score:-3.11|P-value:9.27E-4|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YDL196W(YDL196W_d)|FD-Score:-3.75|P-value:8.87E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL195C(YJL195C_d)|FD-Score:-5.18|P-value:1.12E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTH1(YPR107C)|FD-Score:-4.61|P-value:2.01E-6|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARP7(YPR034W)|FD-Score:-3.23|P-value:6.14E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC24(YAL041W)|FD-Score:-5.3|P-value:5.76E-8|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:GCD10(YNL062C)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.06||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:4.91|P-value:4.64E-7|Clearance:0.57||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HYP2(YEL034W)|FD-Score:-3.54|P-value:2.00E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:INO80(YGL150C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.12||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:IRR1(YIL026C)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MNP1(YGL068W)|FD-Score:4.33|P-value:7.37E-6|Clearance:0.18||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NOP56(YLR197W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.05||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUS1(YDL193W)|FD-Score:-5.54|P-value:1.49E-8|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRI1(YIR008C)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.14||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RER2(YBR002C)|FD-Score:-4.75|P-value:1.02E-6|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB7(YDR404C)|FD-Score:-4.51|P-value:3.26E-6|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC10(YHR143W-A)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPO26(YPR187W)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RSC4(YKR008W)|FD-Score:-3.28|P-value:5.13E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC6(YCR052W)|FD-Score:-4.25|P-value:1.07E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:-3.13|P-value:8.72E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC11(YIR022W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.04||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SEC6(YIL068C)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.53||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SRP72(YPL210C)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.15||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:SUI2(YJR007W)|FD-Score:-3.52|P-value:2.14E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUP35(YDR172W)|FD-Score:-3.11|P-value:9.27E-4|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YDL196W(YDL196W_d)|FD-Score:-3.75|P-value:8.87E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL195C(YJL195C_d)|FD-Score:-5.18|P-value:1.12E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTH1(YPR107C)|FD-Score:-4.61|P-value:2.01E-6|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24282180
Download HOP data (tab-delimited text)  (excel)
Gene:BAG7(YOR134W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:DBP3(YGL078C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DSE2(YHR143W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ENV10(YLR065C)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:LCL1(YPL056C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:MCR1(YKL150W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MRF1(YGL143C)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:NBP2(YDR162C)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:RTG3(YBL103C)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:SAM37(YMR060C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SYO1(YDL063C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRP2(YER090W)|FD-Score:5.3|P-value:5.75E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:YGL218W(YGL218W_d)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR054W(YGR054W)|FD-Score:-3.86|P-value:5.59E-5||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YLR111W(YLR111W_d)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL079W(YOL079W_d)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:BAG7(YOR134W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:DBP3(YGL078C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DSE2(YHR143W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ENV10(YLR065C)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:LCL1(YPL056C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:MCR1(YKL150W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MRF1(YGL143C)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:NBP2(YDR162C)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:RTG3(YBL103C)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:SAM37(YMR060C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SYO1(YDL063C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TRP2(YER090W)|FD-Score:5.3|P-value:5.75E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:YGL218W(YGL218W_d)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR054W(YGR054W)|FD-Score:-3.86|P-value:5.59E-5||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YLR111W(YLR111W_d)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL079W(YOL079W_d)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR013W4.914.64E-70.57GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YGL068W4.337.37E-60.18MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YIL068C4.151.67E-50.53SEC6Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
YGL150C3.621.47E-40.12INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YNL062C3.502.31E-40.06GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YIR022W3.452.84E-40.04SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YLR197W3.403.33E-40.05NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YIR008C3.354.06E-40.14PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YPL210C3.216.63E-40.15SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YIL019W3.060.001120.04FAF1Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
YGR128C3.020.001270.16UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR460W2.860.002120.04TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YGL065C2.820.002410.10ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YIL046W2.720.003260.07MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YJL019W2.650.004000.04MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER090W5.305.75E-8TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YLR111W_d4.111.97E-5YLR111W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL143C4.062.42E-5MRF1Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability
YDL063C3.661.27E-4SYO1Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YGL078C3.651.33E-4DBP3RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype
YKL211C3.591.67E-4TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YPL056C_p3.542.02E-4LCL1_pPutative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan
YKL150W3.413.25E-4MCR1Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis
YMR060C3.354.05E-4SAM37Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
YHR143W3.354.08E-4DSE2Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
YOR134W3.255.86E-4BAG7Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p
YGL218W_d3.158.17E-4YGL218W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance
YOL079W_d3.119.32E-4YOL079W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBL103C3.119.49E-4RTG3Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways
YJR061W_p3.040.00117YJR061W_pPutative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3243
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1484.44E-30SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0961538
0.1344.38E-25SGTC_22619010538 184.7 μMChembridge (Fragment library)64572500.135593
0.1291.85E-23SGTC_11594092-0538 61.2 μMChemDiv (Drug-like library)7173010.109375
0.1271.01E-22SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.109375
0.1271.17E-22SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.115385
0.1202.45E-20SGTC_24685987765 72.9 μMMiscellaneous267720940.138462
0.1195.45E-20SGTC_6800417-1641 114.0 μMChemDiv (Drug-like library)28359900.115942RPP1 & pyrimidine depletion
0.1187.06E-20SGTC_21986589944 200.0 μMChembridge (Fragment library)52902680.0895522
0.1171.34E-19SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.119403
0.1155.77E-19SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.123077
0.1142.16E-18SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.142857
0.1133.64E-18SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.101449
0.1125.13E-18SGTC_2684capsaicin 100.0 μMMiscellaneous15489430.123288
0.1102.80E-17SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.114754TSC3-RPN4
0.1103.32E-17SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.183333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1619st00332688.7 μM0.3333332849032TimTec (Natural product derivative library)215.254421.6422360S ribosome export
SGTC_3111912208849.47 μM0.31147516876704Chembridge (Drug-like library)344.471063.99304
SGTC_9761348-106747.6 μM0.3090915310671ChemDiv (Drug-like library)244.315483.6021460S ribosome export
SGTC_10044084-0029199 μM0.275862716894ChemDiv (Drug-like library)323.412083.22414
SGTC_22597964009200 μM0.27451313134Chembridge (Fragment library)216.232582.7513superoxide
SGTC_7371950-011757.9 μM0.2741945393513ChemDiv (Drug-like library)321.39623.37314
SGTC_22937624732200 μM0.267857894453Chembridge (Fragment library)222.26361.77914
SGTC_10462001-0062216 μM0.261538739778ChemDiv (Drug-like library)341.427363.60714
SGTC_7800109-0199192 μM0.26610414ChemDiv (Drug-like library)208.258483.75611
SGTC_2789528111271.43 μM0.259259765948Chembridge (Drug-like library)308.19363.76403