9134489

3-(3-chlorophenyl)-2,8,8-trimethyl-7,9-dihydropyrazolo[5,1-c][1,2,4]benzotriazin-6-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3244
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 16584271
SMILES CC1=NN2C3=C(C(=O)CC(C3)(C)C)N=NC2=C1C4=CC(=CC=C4)Cl
Standardized SMILES Cc1nn2c3CC(C)(C)CC(=O)c3nnc2c1c4cccc(Cl)c4
Molecular weight 340.8068
ALogP 3.52
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.09
% growth inhibition (Hom. pool) 3.38


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16584271
Download HIP data (tab-delimited text)  (excel)
Gene:APC1(YNL172W)|FD-Score:3.51|P-value:2.28E-4|Clearance:0.08||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:ARC35(YNR035C)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.13||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CCT6(YDR188W)|FD-Score:-4.87|P-value:5.53E-7|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:DAD2(YKR083C)|FD-Score:3.91|P-value:4.70E-5|Clearance:0.15||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:HEM4(YOR278W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.05||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:ILV5(YLR355C)|FD-Score:3.42|P-value:3.13E-4|Clearance:0.08||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IPL1(YPL209C)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.05||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:KRI1(YNL308C)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.05||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MED6(YHR058C)|FD-Score:3.72|P-value:9.99E-5|Clearance:0.05||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:PRP43(YGL120C)|FD-Score:-3.79|P-value:7.66E-5|Clearance:0||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RRN7(YJL025W)|FD-Score:3.87|P-value:5.47E-5|Clearance:0.15||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC13(YLR208W)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.01||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SNP1(YIL061C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.02||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:STU2(YLR045C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.09||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:WRS1(YOL097C)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.04||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGR115C(YGR115C_d)|FD-Score:-4.54|P-value:2.76E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:APC1(YNL172W)|FD-Score:3.51|P-value:2.28E-4|Clearance:0.08||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:ARC35(YNR035C)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.13||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CCT6(YDR188W)|FD-Score:-4.87|P-value:5.53E-7|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:DAD2(YKR083C)|FD-Score:3.91|P-value:4.70E-5|Clearance:0.15||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:HEM4(YOR278W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.05||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:ILV5(YLR355C)|FD-Score:3.42|P-value:3.13E-4|Clearance:0.08||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IPL1(YPL209C)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.05||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:KRI1(YNL308C)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.05||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MED6(YHR058C)|FD-Score:3.72|P-value:9.99E-5|Clearance:0.05||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:PRP43(YGL120C)|FD-Score:-3.79|P-value:7.66E-5|Clearance:0||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RRN7(YJL025W)|FD-Score:3.87|P-value:5.47E-5|Clearance:0.15||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC13(YLR208W)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.01||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SNP1(YIL061C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.02||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:STU2(YLR045C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.09||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:WRS1(YOL097C)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.04||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGR115C(YGR115C_d)|FD-Score:-4.54|P-value:2.76E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16584271
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ADE3(YGR204W)|FD-Score:15|P-value:3.62E-51||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADE4(YMR300C)|FD-Score:8.17|P-value:1.50E-16||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADE5,7(YGL234W)|FD-Score:11.1|P-value:9.87E-29||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:ADE6(YGR061C)|FD-Score:14.2|P-value:6.36E-46||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADE8(YDR408C)|FD-Score:16.1|P-value:6.51E-59||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AKR1(YDR264C)|FD-Score:5.51|P-value:1.75E-8||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:API2(YDR525W_d)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ATG15(YCR068W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:AVT6(YER119C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BPH1(YCR032W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD19(YJL188C_d)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD23(YCR047C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:COG5(YNL051W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR9(YOL145C)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DAL81(YIR023W)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ERI1(YPL096C-A)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FAA1(YOR317W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FAA2(YER015W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:GLK1(YCL040W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GLN3(YER040W)|FD-Score:-4|P-value:3.13E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:HTA2(YBL003C)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:MAC1(YMR021C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAD2(YJL030W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MFG1(YDL233W_p)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MTC4(YBR255W)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NUR1(YDL089W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PAN6(YIL145C)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC Gene:PEP7(YDR323C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PRY1(YJL079C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPS16B(YDL083C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SHM2(YLR058C)|FD-Score:9.23|P-value:1.36E-20||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SMI1(YGR229C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF5(YBR289W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SOD1(YJR104C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:THO2(YNL139C)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TPS2(YDR074W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:VID24(YBR105C)|FD-Score:-4.35|P-value:6.81E-6||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA16(YHR026W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:WSS1(YHR134W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAL018C(YAL018C_p)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Putative protein of unknown function Gene:YBL008W-A(YBL008W-A_p)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR012C(YBR012C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR277C(YBR277C_d)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCL002C(YCL002C_p)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR444W(YDR444W_p)|FD-Score:-3.99|P-value:3.26E-5||SGD DESC:Putative hydrolase acting on ester bonds Gene:YER186C(YER186C_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function Gene:YGR273C(YGR273C_p)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHP1(YDR451C)|FD-Score:-5.72|P-value:5.44E-9||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIR035C(YIR035C_p)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL070C(YJL070C_p)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YKL023W(YKL023W_p)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLL020C(YLL020C_d)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YOL035C(YOL035C_d)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL131W(YOL131W_p)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Putative protein of unknown function Gene:YPR063C(YPR063C_p)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:ER-localized protein of unknown function Gene:YPR098C(YPR098C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:AAT2(YLR027C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ADE3(YGR204W)|FD-Score:15|P-value:3.62E-51||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADE4(YMR300C)|FD-Score:8.17|P-value:1.50E-16||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADE5,7(YGL234W)|FD-Score:11.1|P-value:9.87E-29||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:ADE6(YGR061C)|FD-Score:14.2|P-value:6.36E-46||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADE8(YDR408C)|FD-Score:16.1|P-value:6.51E-59||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AKR1(YDR264C)|FD-Score:5.51|P-value:1.75E-8||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:API2(YDR525W_d)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ATG15(YCR068W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:AVT6(YER119C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BPH1(YCR032W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD19(YJL188C_d)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD23(YCR047C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:COG5(YNL051W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR9(YOL145C)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DAL81(YIR023W)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ERI1(YPL096C-A)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FAA1(YOR317W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FAA2(YER015W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:GLK1(YCL040W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GLN3(YER040W)|FD-Score:-4|P-value:3.13E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:HTA2(YBL003C)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:MAC1(YMR021C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAD2(YJL030W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MFG1(YDL233W_p)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MTC4(YBR255W)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NUR1(YDL089W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PAN6(YIL145C)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC Gene:PEP7(YDR323C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PRY1(YJL079C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPS16B(YDL083C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SHM2(YLR058C)|FD-Score:9.23|P-value:1.36E-20||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SMI1(YGR229C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF5(YBR289W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SOD1(YJR104C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:THO2(YNL139C)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TPS2(YDR074W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:VID24(YBR105C)|FD-Score:-4.35|P-value:6.81E-6||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA16(YHR026W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:WSS1(YHR134W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAL018C(YAL018C_p)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Putative protein of unknown function Gene:YBL008W-A(YBL008W-A_p)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR012C(YBR012C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR277C(YBR277C_d)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCL002C(YCL002C_p)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR444W(YDR444W_p)|FD-Score:-3.99|P-value:3.26E-5||SGD DESC:Putative hydrolase acting on ester bonds Gene:YER186C(YER186C_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function Gene:YGR273C(YGR273C_p)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHP1(YDR451C)|FD-Score:-5.72|P-value:5.44E-9||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIR035C(YIR035C_p)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL070C(YJL070C_p)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication Gene:YKL023W(YKL023W_p)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLL020C(YLL020C_d)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YOL035C(YOL035C_d)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL131W(YOL131W_p)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Putative protein of unknown function Gene:YPR063C(YPR063C_p)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:ER-localized protein of unknown function Gene:YPR098C(YPR098C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKR083C3.914.70E-50.15DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YJL025W3.875.47E-50.15RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YHR058C3.729.99E-50.05MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
YOR278W3.671.21E-40.05HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YLR045C3.621.49E-40.09STU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
YIL061C3.532.11E-40.02SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YNL172W3.512.28E-40.08APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YLR355C3.423.13E-40.08ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YNR035C3.344.13E-40.13ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YOL097C3.226.51E-40.04WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YLR208W3.177.55E-40.01SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YPL209C3.177.69E-40.05IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
YNL308C3.129.04E-40.05KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YPR165W3.070.001080.05RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YJL054W3.010.001290.00TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR408C16.106.51E-59ADE8Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
YGR204W15.003.62E-51ADE3Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
YGR061C14.206.36E-46ADE6Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
YGL234W11.109.87E-29ADE5,7Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
YLR058C9.231.36E-20SHM2Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
YMR300C8.171.50E-16ADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
YDR264C5.511.75E-8AKR1Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication
YBL008W-A_p5.071.99E-7YBL008W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YCL002C_p4.602.14E-6YCL002C_pPutative protein of unknown function; YCL002C is not an essential gene
YBR277C_d4.464.18E-6YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
YOL035C_d4.434.62E-6YOL035C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJL070C_p4.171.51E-5YJL070C_pPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
YOR317W4.131.81E-5FAA1Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication
YLR027C4.111.94E-5AAT2Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
YPL096C-A4.111.95E-5ERI1Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein

GO enrichment analysis for SGTC_3244
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4403.57E-278SGTC_1230083-0120 66.7 μMChemDiv (Drug-like library)160017010.0531915
0.0992.35E-14SGTC_21425622915 118.5 μMChembridge (Fragment library)53221960.0985916
0.0961.36E-13SGTC_7820083-0095 30.5 μMChemDiv (Drug-like library)35917290.101124
0.0952.50E-13SGTC_23889080168 200.0 μMChembridge (Fragment library)159430730.084507
0.0765.91E-9SGTC_2235salicylic acid 500.0 μMMiscellaneous3380.0806452
0.0723.54E-8SGTC_11554088-0394 48.2 μMChemDiv (Drug-like library)23262480.0657895
0.0714.09E-8SGTC_698k064-0052 127.0 μMChemDiv (Drug-like library)50887510.0941176DNA damage response
0.0705.89E-8SGTC_5491661-1316 519.0 μMChemDiv (Drug-like library)28572820.117021
0.0691.23E-7SGTC_1957st077035 62.4 μMTimTec (Natural product derivative library)7055930.134146
0.0663.65E-7SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.0666667translation
0.0655.05E-7SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.112903cell wall signaling
0.0655.64E-7SGTC_28287998861 71.4 μMChembridge (Drug-like library)29881880.1125
0.0656.08E-7SGTC_2405hr heat shock (37°C) + Streptovitacin 300.0 μMMiscellaneous914670.0886076heat shock/prefoldin
0.0612.59E-6SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.101449heat shock/prefoldin
0.0604.74E-6SGTC_20995377090 177.4 μMChembridge (Fragment library)22438290.0810811

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3333914182012.09 μM0.2739731939433Chembridge (Drug-like library)349.472583.97404
SGTC_3343914837918.98 μM0.2666671955036Chembridge (Drug-like library)349.472583.96804
SGTC_334591484103.56 μM0.2631584903596Chembridge (Drug-like library)413.514783.9715
SGTC_1846st05624414.8 μM0.260875393182TimTec (Natural product derivative library)286.709744.24713
SGTC_3235913260249.47 μM0.2567574912240Chembridge (Drug-like library)352.430163.31605
SGTC_3280914096149.47 μM0.2567574901226Chembridge (Drug-like library)355.864483.5090460S ribosome export
SGTC_3393091-483371.4 μM0.2537313240077ChemDiv (Drug-like library)272.686463.1713iron homeostasis
SGTC_3328914062429.04 μM0.2535214904392Chembridge (Drug-like library)335.4463.33104
SGTC_3339914588314.94 μM0.2531652030670Chembridge (Drug-like library)379.498563.43515
SGTC_3326914006416.38 μM0.251868434Chembridge (Drug-like library)379.498563.42815