9135460

N-(4-chloro-2-methylphenyl)-5-fluoro-2-methoxybenzenesulfonamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3246
Screen concentration 33.0 μM
Source Chembridge (Drug-like library)
PubChem CID 41446951
SMILES CC1=C(C=CC(=C1)Cl)NS(=O)(=O)C2=C(C=CC(=C2)F)OC
Standardized SMILES COc1ccc(F)cc1S(=O)(=O)Nc2ccc(Cl)cc2C
Molecular weight 329.7743
ALogP 3.66
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.45
% growth inhibition (Hom. pool) 2.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 41446951
Download HIP data (tab-delimited text)  (excel)
Gene:COP1(YDL145C)|FD-Score:-4.21|P-value:1.30E-5|Clearance:0||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:GRS1(YBR121C)|FD-Score:3.23|P-value:6.30E-4|Clearance:0.13||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:MIM1(YOL026C)|FD-Score:-4.36|P-value:6.51E-6|Clearance:0||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:MTR4(YJL050W)|FD-Score:-3.32|P-value:4.55E-4|Clearance:0||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NSE1(YLR007W)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.06||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PRE10(YOR362C)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP3(YDR473C)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.13||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:ROK1(YGL171W)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:UBA1(YKL210W)|FD-Score:3.36|P-value:3.93E-4|Clearance:0.13||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:COP1(YDL145C)|FD-Score:-4.21|P-value:1.30E-5|Clearance:0||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:GRS1(YBR121C)|FD-Score:3.23|P-value:6.30E-4|Clearance:0.13||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:MIM1(YOL026C)|FD-Score:-4.36|P-value:6.51E-6|Clearance:0||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:MTR4(YJL050W)|FD-Score:-3.32|P-value:4.55E-4|Clearance:0||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NSE1(YLR007W)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.06||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PRE10(YOR362C)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP3(YDR473C)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.13||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:ROK1(YGL171W)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:UBA1(YKL210W)|FD-Score:3.36|P-value:3.93E-4|Clearance:0.13||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 41446951
Download HOP data (tab-delimited text)  (excel)
Gene:ANT1(YPR128C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARG2(YJL071W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:BTS1(YPL069C)|FD-Score:-5.5|P-value:1.89E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BZZ1(YHR114W)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CAR1(YPL111W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CIN1(YOR349W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COG6(YNL041C)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYB5(YNL111C)|FD-Score:5.05|P-value:2.17E-7||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:ELP2(YGR200C)|FD-Score:-5.15|P-value:1.31E-7||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ERV2(YPR037C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:GCV3(YAL044C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GDB1(YPR184W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:HOT1(YMR172W)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p Gene:LAG2(YOL025W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MNT4(YNR059W)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:NSR1(YGR159C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:PHO90(YJL198W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VMA3(YEL027W)|FD-Score:-4.1|P-value:2.04E-5||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:WSS1(YHR134W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBR056W(YBR056W_p)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR277C(YBR277C_d)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR099C(YCR099C_p)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Putative protein of unknown function Gene:YHP1(YDR451C)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIL134C-A(YIL134C-A_p)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL150W(YJL150W_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR460C(YLR460C_p)|FD-Score:4.99|P-value:3.01E-7||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML096W(YML096W_p)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YNL022C(YNL022C_p)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNL226W(YNL226W_d)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR131C(YOR131C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR200W(YOR200W_d)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:ANT1(YPR128C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARG2(YJL071W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:BTS1(YPL069C)|FD-Score:-5.5|P-value:1.89E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BZZ1(YHR114W)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CAR1(YPL111W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CIN1(YOR349W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COG6(YNL041C)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYB5(YNL111C)|FD-Score:5.05|P-value:2.17E-7||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:ELP2(YGR200C)|FD-Score:-5.15|P-value:1.31E-7||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ERV2(YPR037C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:GCV3(YAL044C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GDB1(YPR184W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:HOT1(YMR172W)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p Gene:LAG2(YOL025W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MNT4(YNR059W)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:NSR1(YGR159C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:PHO90(YJL198W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VMA3(YEL027W)|FD-Score:-4.1|P-value:2.04E-5||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:WSS1(YHR134W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBR056W(YBR056W_p)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR277C(YBR277C_d)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR099C(YCR099C_p)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Putative protein of unknown function Gene:YHP1(YDR451C)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIL134C-A(YIL134C-A_p)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL150W(YJL150W_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR460C(YLR460C_p)|FD-Score:4.99|P-value:3.01E-7||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML096W(YML096W_p)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YNL022C(YNL022C_p)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNL226W(YNL226W_d)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR131C(YOR131C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR200W(YOR200W_d)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL210W3.363.93E-40.13UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YDR473C3.275.37E-40.13PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YBR121C3.236.30E-40.13GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YLR007W3.099.95E-40.06NSE1Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YLR045C3.030.001220.04STU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
YGR265W_d2.990.001410.12YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YML049C2.860.002090.06RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
YJR076C2.810.002510.00CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YKL059C2.800.002550.07MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YOR204W2.730.003150.02DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YBL035C2.720.003300.02POL12B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
YOR254C2.700.003510.04SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YPR180W2.660.003900.07AOS1Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YFL024C2.590.004770.11EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YMR213W2.480.006540.03CEF1Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL111C5.052.17E-7CYB5Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
YLR460C_p4.993.01E-7YLR460C_pMember of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine
YHR134W4.121.92E-5WSS1Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response
YBR277C_d4.013.02E-5YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
YPR128C3.993.34E-5ANT1Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
YNR059W3.778.13E-5MNT4Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation
YMR172W3.749.09E-5HOT1Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
YBR056W_p3.571.80E-4YBR056W_pPutative glycoside hydrolase of the mitochondrial intermembrane space
YAL044C3.532.06E-4GCV3H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
YCR099C_p3.324.42E-4YCR099C_pPutative protein of unknown function
YOR131C_p3.324.49E-4YOR131C_pPutative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress
YPR037C3.324.50E-4ERV2Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER
YOL025W3.265.59E-4LAG2Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1
YOR349W3.236.23E-4CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YJL150W_d3.206.76E-4YJL150W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_3246
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0773.51E-9SGTC_14284049-0204 61.7 μMChemDiv (Drug-like library)X14280.278689
0.0741.43E-8SGTC_32989116858 71.4 μMChembridge (Drug-like library)25069420.253968
0.0715.41E-8SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.147059
0.0691.17E-7SGTC_2545solidagenone 89.6 μMMicrosource (Natural product library)67085720.0740741
0.0681.41E-7SGTC_2551apiole 73.9 μMMicrosource (Natural product library)106590.140625
0.0648.65E-7SGTC_487nimodipine 119.0 μMMiscellaneous44970.107143
0.0612.81E-6SGTC_216k038-0035 28.4 μMChemDiv (Drug-like library)44442310.0422535ubiquinone biosynthesis & proteasome
0.0612.95E-6SGTC_1753st043925 64.0 μMTimTec (Natural product derivative library)6701810.152778
0.0571.02E-5SGTC_1736st036774 51.0 μMTimTec (Natural product derivative library)12722470.0666667
0.0561.47E-5SGTC_13521496-0991 99.0 μMChemDiv (Drug-like library)30000390.0769231tubulin folding & SWR complex
0.0561.57E-5SGTC_2414st077232 92.9 μMTimTec (Natural product derivative library)7298010.0735294
0.0561.68E-5SGTC_14790549-0158 134.0 μMChemDiv (Drug-like library)54402020.0694444
0.0552.40E-5SGTC_13893405-0187 19.0 μMChemDiv (Drug-like library)7998900.056338
0.0542.85E-5SGTC_2744captopril 92.0 μMMiscellaneous440930.028571460S ribosome export
0.0535.09E-5SGTC_14013807-4679 7.6 μMChemDiv (Drug-like library)32914560.0533333tubulin folding & SWR complex

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3076911618749.47 μM0.56862727241210Chembridge (Drug-like library)327.783263.20925
SGTC_1743553-038974.3 μM0.470588738071ChemDiv (Drug-like library)287.78563.25414
SGTC_12580671-004357.1 μM0.4423084298767ChemDiv (Drug-like library)296.772522.72124
SGTC_14324049-029868.4 μM0.423077741646ChemDiv (Drug-like library)299.2931462.71216
SGTC_2870903852812.99 μM0.3684216463316Chembridge (Drug-like library)325.810443.17804
SGTC_3434130-282729.8 μM0.3518521118244ChemDiv (Drug-like library)354.6118834.51614
SGTC_2853901504258.44 μM0.356457456Chembridge (Drug-like library)307.364783.00125
SGTC_3176910658249.47 μM0.32203417018027Chembridge (Drug-like library)275.730163.74812
SGTC_3134912745449.47 μM0.32142924262820Chembridge (Drug-like library)273.30551.17826
SGTC_10034049-0218100 μM0.320755295161ChemDiv (Drug-like library)279.3298633.49514